Rw7G041030

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009066
Physical Location & Seq
Reverse (-)
63986759 .. 63988389
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041030.1

Sequence Viewer

Length: 1059 bp
ATGGCTATCATCAATCACTGCGCGTACCTATTACTTATCTCTCTCTTCTTCATAATATCCTTGTTCGGGTTATCCAATGCAGCCACAAATTATCATGTGATGAAATATGACACCCAAGCATTTGTCAAGACATGGGCAGCAGCATGCAGCTCTGCTCAGAAAGCCACCATGCATGTACCTGCAGTGTTCAGAGGCCCTTGCAAGAGTAGAATTAACGTTCAGATATACGGAACCTTGATTGCCCCGACTGATTATTGGGCTCTGGGAAACTCCGGCTATTGGATCTTATTTATTAAGGTCAATAGACTTGCATTTTTCGGTGGTACACTCGACGCCAAGGGAGCTGGCATTTGGGCTTGCAGGAAATCCGGAAAGAGCAGCCCTGTTGGAGCTAGGTCGATAACGTTCAATTGGGCAAATGACGTTGTGATTAGTGGTTTAGTATCCATCAACAGTCAGCAAACTCATCTTGTGATCAACAGCTGCAACAATGTGGTGTTCCGAAACGTAAAGCTGGTTGCTCCGGATGACAGCCCCAACACTGATGGCATTCGAGTGCAGATCTCAACTGGTGTTACTATCACTGGCGCAACCTTGCAAACCGGAGACGATTGTGAATCCAACCTCTACATGAGTAACATCAACATTGGTAGTCTAGGCAACGCAGTGAACGAAGCTGGGGTCCAAAATGTGACGTTGACGAATGCAATTTTCACTGGATCGGACAATGGAGTACGAATAAAGTCATGGGCCAGGCCCAGCAATAGTTTTATTTGTCAAGTGACATACAAGAATATACAAGGGACGTCGGCAAAGGCGGAGGCAGTAACATTTGATTGCAGCCCAAGTAATCCGTGCAGAGGGATTAGATTGCAAGACATCAAGCTTACATACATGAATCCGGTAGCCACATCTTCATGCAAGAACATTCGTGGAAGCACCACCGGAGTATACTCATGCCGGTACTCCGGTGGTACCACGGTTGATATTTTCCAATTCTTTGAAACCTTAATTAGTTCCCTTTTCCTTCATTTTCCAGGTTGGTTTTTGATTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

352

Amino Acids

38.17

Weight (kDa)

9.02

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 49 - 251 3.9e-34 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 258 - 311 2.4e-08 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 809
Acc36I ACCTGC 1 cut(s) 187
Acc65I GGTACC 1 cut(s) 974
AccB1I GGYRCC 1 cut(s) 974
AccI GTMKAC 1 cut(s) 951
AccII CGCG 1 cut(s) 23
AccIII TCCGGA 2 cut(s) 368, 523
AciI CCGC 1 cut(s) 818
AclI AACGTT 2 cut(s) 216, 404
AclWI GGATC 2 cut(s) 290, 727
AcyI GRCGYC 2 cut(s) 333, 806
AfaI GTAC 6 cut(s) 26, 177, 325, 735, 965, 976
AfiI CCNNNNNNNGG 3 cut(s) 66, 279, 860
AgsI TTSAA 2 cut(s) 409, 1004
AjnI CCWGG 2 cut(s) 752, 1036
AluBI AGCT 7 cut(s) 150, 344, 392, 483, 514, 677, 886
AluI AGCT 7 cut(s) 150, 344, 392, 483, 514, 677, 886
Alw26I GTCTC 1 cut(s) 600
AlwI GGATC 2 cut(s) 290, 727
Aor13HI TCCGGA 2 cut(s) 368, 523
AoxI GGCC 3 cut(s) 193, 750, 755
ApeKI GCWGC 7 cut(s) 80, 137, 140, 147, 378, 483, 840
ArsI GACNNNNNNTTYG 2 cut(s) 666, 698
Asp718I GGTACC 1 cut(s) 974
AspLEI GCGC 2 cut(s) 23, 590
AspS9I GGNCC 4 cut(s) 194, 682, 750, 756
AvaII GGWCC 1 cut(s) 682
BaeI ACNNNNGTAYC 4 cut(s) 955, 966, 988, 999
BanI GGYRCC 1 cut(s) 974
BanII GRGCYC 1 cut(s) 262
BbvI GCAGC 7 cut(s) 92, 149, 152, 159, 390, 470, 852
BccI CCATC 2 cut(s) 455, 539
BciT130I CCWGG 2 cut(s) 754, 1038
BciVI GTATCC 1 cut(s) 454
BclI TGATCA 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 600
BfaI CTAG 2 cut(s) 393, 656
BfmI CTRYAG 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 187
BfuI GTATCC 1 cut(s) 454
BglII AGATCT 1 cut(s) 561
BisI GCNGC 7 cut(s) 81, 138, 141, 148, 379, 484, 841
BlsI GCNGC 7 cut(s) 82, 139, 142, 149, 380, 485, 842
Bme1390I CCNGG 2 cut(s) 754, 1038
Bme18I GGWCC 1 cut(s) 682
BmgT120I GGNCC 4 cut(s) 194, 682, 750, 756
BmiI GGNNCC 3 cut(s) 232, 683, 976
BmrFI CCNGG 2 cut(s) 754, 1038
BsaHI GRCGYC 2 cut(s) 333, 806
BsaJI CCNNGG 2 cut(s) 336, 978
BsaWI WCCGGW 6 cut(s) 368, 523, 602, 901, 944, 968
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 279, 860
Bse118I RCCGGY 1 cut(s) 960
Bse1I ACTGG 3 cut(s) 574, 589, 721
BseAI TCCGGA 2 cut(s) 368, 523
BseBI CCWGG 2 cut(s) 754, 1038
BseDI CCNNGG 2 cut(s) 336, 978
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 3 cut(s) 66, 279, 860
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 3 cut(s) 574, 589, 721
BseXI GCAGC 7 cut(s) 92, 149, 152, 159, 390, 470, 852
BseYI CCCAGC 2 cut(s) 677, 758
BsgI GTGCAG 2 cut(s) 578, 877
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 195, 752, 757
BshNI GGYRCC 1 cut(s) 974
BsiSI CCGG 8 cut(s) 273, 369, 524, 603, 902, 945, 961, 969
BslFI GGGAC 1 cut(s) 817
BslI CCNNNNNNNGG 3 cut(s) 66, 279, 860
BsmAI GTCTC 1 cut(s) 600
BsmBI CGTCTC 1 cut(s) 600
BsmFI GGGAC 1 cut(s) 817
BsmI GAATGC 2 cut(s) 549, 709
BsnI GGCC 3 cut(s) 195, 752, 757
Bsp1286I GDGCHC 1 cut(s) 262
Bsp13I TCCGGA 2 cut(s) 368, 523
Bsp143I GATC 4 cut(s) 282, 474, 561, 719
BspACI CCGC 1 cut(s) 818
BspANI GGCC 3 cut(s) 195, 752, 757
BspCNI CTCAG 1 cut(s) 169
BspEI TCCGGA 2 cut(s) 368, 523
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 232, 683, 976
BspMAI CTGCAG 1 cut(s) 184
BspMI ACCTGC 1 cut(s) 187
BspPI GGATC 2 cut(s) 290, 727
BspT107I GGYRCC 1 cut(s) 974
BsrFI RCCGGY 1 cut(s) 960
BsrI ACTGG 3 cut(s) 574, 589, 721
BssAI RCCGGY 1 cut(s) 960
BssECI CCNNGG 2 cut(s) 336, 978
BssMI GATC 4 cut(s) 282, 474, 561, 719
BssNAI GTATAC 1 cut(s) 952
BssNI GRCGYC 2 cut(s) 333, 806
BssT1I CCWWGG 1 cut(s) 336
Bst1107I GTATAC 1 cut(s) 952
Bst2UI CCWGG 2 cut(s) 754, 1038
Bst4CI ACNGT 2 cut(s) 455, 982
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 2 cut(s) 333, 806
BstC8I GCNNGC 3 cut(s) 145, 346, 358
BstDEI CTNAG 1 cut(s) 156
BstDSI CCRYGG 1 cut(s) 978
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 2 cut(s) 23, 590
BstKTI GATC 4 cut(s) 285, 477, 564, 722
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 4 cut(s) 282, 474, 561, 719
BstMWI GCNNNNNNNGC 2 cut(s) 161, 341
BstNI CCWGG 2 cut(s) 754, 1038
BstNSI RCATGY 2 cut(s) 147, 176
BstSCI CCNGG 2 cut(s) 752, 1036
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 7 cut(s) 92, 149, 152, 159, 390, 470, 852
BstX2I RGATCY 2 cut(s) 282, 561
BstYI RGATCY 2 cut(s) 282, 561
BstZ17I GTATAC 1 cut(s) 952
BsuI GTATCC 1 cut(s) 454
BsuRI GGCC 3 cut(s) 195, 752, 757
BtgI CCRYGG 1 cut(s) 978
BtsCI GGATG 1 cut(s) 532
BtsI GCAGTG 3 cut(s) 16, 189, 672
BtsIMutI CAGTG 6 cut(s) 16, 189, 540, 582, 672, 714
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 3 cut(s) 145, 346, 358
CfoI GCGC 2 cut(s) 23, 590
Cfr10I RCCGGY 1 cut(s) 960
Cfr13I GGNCC 4 cut(s) 194, 682, 750, 756
CseI GACGC 1 cut(s) 341
Csp6I GTAC 6 cut(s) 25, 176, 324, 734, 964, 975
CviQI GTAC 6 cut(s) 25, 176, 324, 734, 964, 975
DdeI CTNAG 1 cut(s) 156
DpnI GATC 4 cut(s) 284, 476, 563, 721
DpnII GATC 4 cut(s) 282, 474, 561, 719
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
EciI GGCGGA 1 cut(s) 833
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 262
Eco47I GGWCC 1 cut(s) 682
EcoO109I RGGNCCY 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 752, 1036
EcoT14I CCWWGG 1 cut(s) 336
EcoT22I ATGCAT 1 cut(s) 174
EcoT38I GRGCYC 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 336
Esp3I CGTCTC 1 cut(s) 600
FaqI GGGAC 1 cut(s) 817
FbaI TGATCA 1 cut(s) 474
FblI GTMKAC 1 cut(s) 951
Fnu4HI GCNGC 7 cut(s) 81, 138, 141, 148, 379, 484, 841
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 7 cut(s) 81, 138, 141, 148, 379, 484, 841
FspBI CTAG 2 cut(s) 393, 656
GlaI GCGC 2 cut(s) 22, 589
GluI GCNGC 7 cut(s) 81, 138, 141, 148, 379, 484, 841
GsaI CCCAGC 2 cut(s) 681, 762
HaeIII GGCC 3 cut(s) 195, 752, 757
HapII CCGG 8 cut(s) 273, 369, 524, 603, 902, 945, 961, 969
HgaI GACGC 1 cut(s) 341
HhaI GCGC 2 cut(s) 23, 590
Hin1I GRCGYC 2 cut(s) 333, 806
Hin6I GCGC 2 cut(s) 21, 588
HinP1I GCGC 2 cut(s) 21, 588
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HindIII AAGCTT 1 cut(s) 884
HinfI GANTC 2 cut(s) 617, 898
HpaII CCGG 8 cut(s) 273, 369, 524, 603, 902, 945, 961, 969
Hpy166II GTNNAC 4 cut(s) 326, 670, 699, 952
Hpy188I TCNGA 5 cut(s) 159, 191, 222, 503, 724
Hpy188III TCNNGA 3 cut(s) 127, 369, 524
Hpy8I GTNNAC 4 cut(s) 326, 670, 699, 952
Hpy99I CGWCG 2 cut(s) 335, 811
HpyAV CCTTC 1 cut(s) 1037
HpyCH4III ACNGT 2 cut(s) 455, 982
HpyCH4IV ACGT 6 cut(s) 216, 404, 423, 507, 695, 806
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 341
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 6 cut(s) 216, 404, 423, 507, 695, 806
Hsp92I GRCGYC 2 cut(s) 333, 806
HspAI GCGC 2 cut(s) 21, 588
Kpn2I TCCGGA 2 cut(s) 368, 523
KpnI GGTACC 1 cut(s) 978
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 4 cut(s) 282, 474, 561, 719
LmnI GCTCC 3 cut(s) 341, 389, 526
Lsp1109I GCAGC 7 cut(s) 92, 149, 152, 159, 390, 470, 852
MaeI CTAG 2 cut(s) 393, 656
MaeII ACGT 6 cut(s) 216, 404, 423, 507, 695, 806
MaeIII GTNAC 5 cut(s) 574, 635, 691, 781, 826
MalI GATC 4 cut(s) 284, 476, 563, 721
MboI GATC 4 cut(s) 282, 474, 561, 719
MboII GAAGA 3 cut(s) 37, 40, 906
MfeI CAATTG 1 cut(s) 409
MflI RGATCY 2 cut(s) 282, 561
MhlI GDGCHC 1 cut(s) 262
MluCI AATT 6 cut(s) 88, 210, 409, 708, 995, 1011
MmeI TCCRAC 2 cut(s) 367, 645
MnlI CCTC 4 cut(s) 185, 635, 814, 854
Mph1103I ATGCAT 1 cut(s) 174
MroI TCCGGA 2 cut(s) 368, 523
MseI TTAA 4 cut(s) 213, 294, 1010, 1057
MslI CAYNNNNRTG 2 cut(s) 554, 916
MspA1I CMGCKG 1 cut(s) 483
MspI CCGG 8 cut(s) 273, 369, 524, 603, 902, 945, 961, 969
MspR9I CCNGG 2 cut(s) 754, 1038
MunI CAATTG 1 cut(s) 409
Mva1269I GAATGC 2 cut(s) 549, 709
MvaI CCWGG 2 cut(s) 754, 1038
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 161, 341
NdeII GATC 4 cut(s) 282, 474, 561, 719
NlaIV GGNNCC 3 cut(s) 232, 683, 976
NmuCI GTSAC 2 cut(s) 691, 781
NsiI ATGCAT 1 cut(s) 174
NspI RCATGY 2 cut(s) 147, 176
PaeI GCATGC 1 cut(s) 147
PcsI WCGNNNNNNNCGW 1 cut(s) 669
PctI GAATGC 2 cut(s) 549, 709
PfeI GAWTC 2 cut(s) 617, 898
PkrI GCNGC 7 cut(s) 82, 139, 142, 149, 380, 485, 842
Psp1406I AACGTT 2 cut(s) 216, 404
Psp6I CCWGG 2 cut(s) 752, 1036
PspFI CCCAGC 2 cut(s) 677, 758
PspGI CCWGG 2 cut(s) 752, 1036
PspN4I GGNNCC 3 cut(s) 232, 683, 976
PspPI GGNCC 4 cut(s) 194, 682, 750, 756
PstI CTGCAG 1 cut(s) 184
PsuI RGATCY 2 cut(s) 282, 561
PvuII CAGCTG 1 cut(s) 483
RsaI GTAC 6 cut(s) 26, 177, 325, 735, 965, 976
RsaNI GTAC 6 cut(s) 25, 176, 324, 734, 964, 975
RseI CAYNNNNRTG 2 cut(s) 554, 916
SaqAI TTAA 4 cut(s) 213, 294, 1010, 1057
SatI GCNGC 7 cut(s) 81, 138, 141, 148, 379, 484, 841
Sau3AI GATC 4 cut(s) 282, 474, 561, 719
Sau96I GGNCC 4 cut(s) 194, 682, 750, 756
ScrFI CCNGG 2 cut(s) 754, 1038
SduI GDGCHC 1 cut(s) 262
SfcI CTRYAG 1 cut(s) 180
SinI GGWCC 1 cut(s) 682
SmiMI CAYNNNNRTG 2 cut(s) 554, 916
SphI GCATGC 1 cut(s) 147
Sse9I AATT 6 cut(s) 88, 210, 409, 708, 995, 1011
SsiI CCGC 1 cut(s) 818
SspMI CTAG 2 cut(s) 393, 656
StyD4I CCNGG 2 cut(s) 752, 1036
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 2 cut(s) 455, 982
TaiI ACGT 6 cut(s) 219, 407, 426, 510, 698, 809
TaqI TCGA 3 cut(s) 330, 398, 553
TasI AATT 6 cut(s) 88, 210, 409, 708, 995, 1011
TfiI GAWTC 2 cut(s) 617, 898
Tru1I TTAA 4 cut(s) 213, 294, 1010, 1057
Tru9I TTAA 4 cut(s) 213, 294, 1010, 1057
TscAI CASTG 6 cut(s) 23, 189, 547, 589, 672, 721
TseFI GTSAC 2 cut(s) 691, 781
TseI GCWGC 7 cut(s) 80, 137, 140, 147, 378, 483, 840
Tsp45I GTSAC 2 cut(s) 691, 781
TspDTI ATGAA 5 cut(s) 40, 116, 906, 911, 1019
TspGWI ACGGA 2 cut(s) 243, 843
TspRI CASTG 6 cut(s) 23, 189, 547, 589, 672, 721
VpaK11BI GGWCC 1 cut(s) 682
XceI RCATGY 2 cut(s) 147, 176
XmiI GTMKAC 1 cut(s) 951
XspI CTAG 2 cut(s) 393, 656
ZraI GACGTC 1 cut(s) 807
Zsp2I ATGCAT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.