Rorug02G0413500

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0009066
Physical Location & Seq
Reverse (-)
52913676 .. 52915818
2143 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0413500.1

Sequence Viewer

Length: 618 bp
ATGAAAGCATTGCCAGTACTACAACTCTTGTTTCTAGCCACACTTGTGGCCACAACCCAAGGTTCATTCGAACTTGACATAGCCGACCAAATAATCCATCTCCTCCGGCCAAAATCCGGCGTCGGCGGCAGCCAAGTCGGCAACATTTCATGCCTGAGTTGGCGACTGGGAGTCGAGACCCACAACATCATTGACTGGACGACTATTCCAGCTGCTTGTGAAGGCTACGTCGGGCACTACATGCTCGGCGACCAGTACAAAAAAGACTCGGAAGTGGTTACTCATGAGGCCTATGTCTATGCTGAGGGGCTGAACCTCACCGACGATGGTAACAACTTGTGGGTATTTGACATCGATGAGACTACTCTCTCTAATCTGCCGTACTATGCCGAGCATGGATTCGGGGTGGAGGTATATAATTCAACTGCATTCACTGCCTGGGTCTTGGAAGGCATAGCCCCAGCTTTGCCCGAGAGTCTAAAGCTGTACAAGAAGTTATTGGCTCTTGGAATCAAGGTTGCATTCATAACTGGAAGGGAAGAATCTCTGAGAAATGTCACAGAAACCAATCTGAAGAATGTTGGATACGATACCTGGGAAACGCTCATACTAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

205

Amino Acids

22.71

Weight (kDa)

4.79

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 46 - 205 1.3e-58 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 126
AcoI YGGCCR 2 cut(s) 48, 107
AcuI CTGAAG 1 cut(s) 593
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 4 cut(s) 18, 257, 383, 488
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 1 cut(s) 423
AhdI GACNNNNNGTC 1 cut(s) 170
AjnI CCWGG 2 cut(s) 437, 593
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 3 cut(s) 212, 464, 484
AluI AGCT 3 cut(s) 212, 464, 484
Alw26I GTCTC 2 cut(s) 170, 353
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 3 cut(s) 48, 107, 288
ApeKI GCWGC 2 cut(s) 129, 212
AsuHPI GGTGA 1 cut(s) 310
AsuII TTCGAA 1 cut(s) 69
AvaI CYCGRG 1 cut(s) 470
BaeGI GKGCMC 1 cut(s) 237
BalI TGGCCA 1 cut(s) 50
BbvCI CCTCAGC 1 cut(s) 303
BbvI GCAGC 2 cut(s) 141, 199
BccI CCATC 2 cut(s) 105, 320
BceAI ACGGC 1 cut(s) 364
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 2 cut(s) 439, 595
BciVI GTATCC 1 cut(s) 578
BcoDI GTCTC 2 cut(s) 170, 353
BfaI CTAG 1 cut(s) 35
BfuI GTATCC 1 cut(s) 578
BglI GCCNNNNNGGC 1 cut(s) 138
BisI GCNGC 3 cut(s) 127, 130, 213
BlsI GCNGC 3 cut(s) 128, 131, 214
BmcAI AGTACT 1 cut(s) 18
Bme1390I CCNGG 2 cut(s) 439, 595
BmeRI GACNNNNNGTC 1 cut(s) 170
BmeT110I CYCGRG 1 cut(s) 470
BmrFI CCNGG 2 cut(s) 439, 595
BmrI ACTGGG 1 cut(s) 176
BmuI ACTGGG 1 cut(s) 176
Bpu10I CCTNAGC 1 cut(s) 303
Bpu14I TTCGAA 1 cut(s) 69
Bsa29I ATCGAT 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 3 cut(s) 58, 438, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 5 cut(s) 14, 171, 200, 253, 535
Bse3DI GCAATG 1 cut(s) 8
BseBI CCWGG 2 cut(s) 439, 595
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 3 cut(s) 58, 438, 594
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMI GCAATG 1 cut(s) 8
BseMII CTCAG 3 cut(s) 146, 294, 539
BseNI ACTGG 5 cut(s) 14, 171, 200, 253, 535
BseRI GAGGAG 1 cut(s) 92
BseSI GKGCMC 1 cut(s) 237
BseXI GCAGC 2 cut(s) 141, 199
BseYI CCCAGC 1 cut(s) 460
BshFI GGCC 3 cut(s) 50, 109, 290
BshVI ATCGAT 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 2 cut(s) 106, 117
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 2 cut(s) 170, 353
BsmI GAATGC 2 cut(s) 428, 521
BsnI GGCC 3 cut(s) 50, 109, 290
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 470
Bsp119I TTCGAA 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 237
Bsp1407I TGTACA 1 cut(s) 486
BspACI CCGC 1 cut(s) 126
BspANI GGCC 3 cut(s) 50, 109, 290
BspCNI CTCAG 3 cut(s) 147, 295, 540
BspDI ATCGAT 1 cut(s) 354
BspHI TCATGA 1 cut(s) 283
BspT104I TTCGAA 1 cut(s) 69
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 8
BsrGI TGTACA 1 cut(s) 486
BsrI ACTGG 5 cut(s) 14, 171, 200, 253, 535
BssECI CCNNGG 3 cut(s) 58, 438, 594
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 1 cut(s) 58
Bst2UI CCWGG 2 cut(s) 439, 595
BstACI GRCGYC 1 cut(s) 120
BstAPI GCANNNNNTGC 2 cut(s) 241, 434
BstAUI TGTACA 1 cut(s) 486
BstBI TTCGAA 1 cut(s) 69
BstDEI CTNAG 3 cut(s) 155, 303, 548
BstMAI GTCTC 2 cut(s) 170, 353
BstMWI GCNNNNNNNGC 4 cut(s) 126, 138, 241, 434
BstNI CCWGG 2 cut(s) 439, 595
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 2 cut(s) 437, 593
BstSLI GKGCMC 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 141, 199
BstXI CCANNNNNNTGG 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 354
BsuI GTATCC 1 cut(s) 578
BsuRI GGCC 3 cut(s) 50, 109, 290
BsuTUI ATCGAT 1 cut(s) 354
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 1 cut(s) 432
CciI TCATGA 1 cut(s) 283
ClaI ATCGAT 1 cut(s) 354
CseI GACGC 1 cut(s) 109
Csp6I GTAC 4 cut(s) 17, 256, 382, 487
CviAII CATG 4 cut(s) 150, 241, 284, 395
CviQI GTAC 4 cut(s) 17, 256, 382, 487
DdeI CTNAG 3 cut(s) 155, 303, 548
DriI GACNNNNNGTC 1 cut(s) 170
EaeI YGGCCR 2 cut(s) 48, 107
Eam1105I GACNNNNNGTC 1 cut(s) 170
Eco130I CCWWGG 1 cut(s) 58
Eco147I AGGCCT 1 cut(s) 290
Eco31I GGTCTC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 593
Eco88I CYCGRG 1 cut(s) 470
EcoRII CCWGG 2 cut(s) 437, 593
EcoT14I CCWWGG 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 4 cut(s) 153, 244, 287, 398
FatI CATG 4 cut(s) 149, 240, 283, 394
Fnu4HI GCNGC 3 cut(s) 127, 130, 213
Fsp4HI GCNGC 3 cut(s) 127, 130, 213
FspBI CTAG 1 cut(s) 35
GluI GCNGC 3 cut(s) 127, 130, 213
GsaI CCCAGC 1 cut(s) 464
HaeIII GGCC 3 cut(s) 50, 109, 290
HapII CCGG 2 cut(s) 106, 117
HgaI GACGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 4 cut(s) 153, 244, 287, 398
HinfI GANTC 6 cut(s) 171, 266, 399, 475, 510, 542
HpaII CCGG 2 cut(s) 106, 117
HphI GGTGA 1 cut(s) 310
Hpy188I TCNGA 3 cut(s) 271, 549, 573
Hpy188III TCNNGA 2 cut(s) 175, 284
Hpy99I CGWCG 3 cut(s) 125, 233, 326
HpyAV CCTTC 3 cut(s) 215, 443, 528
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 2 cut(s) 428, 521
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 138, 241, 434
HpyF3I CTNAG 3 cut(s) 155, 303, 548
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 4 cut(s) 153, 244, 287, 398
Lsp1109I GCAGC 2 cut(s) 141, 199
MaeI CTAG 1 cut(s) 35
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 3 cut(s) 277, 329, 556
MboII GAAGA 2 cut(s) 551, 586
MhlI GDGCHC 1 cut(s) 237
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 1 cut(s) 418
MluNI TGGCCA 1 cut(s) 50
MlyI GAGTC 3 cut(s) 180, 260, 484
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 5 cut(s) 113, 280, 298, 326, 403
Mox20I TGGCCA 1 cut(s) 50
MscI TGGCCA 1 cut(s) 50
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 212
MspI CCGG 2 cut(s) 106, 117
MspR9I CCNGG 2 cut(s) 439, 595
Mva1269I GAATGC 2 cut(s) 428, 521
MvaI CCWGG 2 cut(s) 439, 595
MwoI GCNNNNNNNGC 4 cut(s) 126, 138, 241, 434
NlaIII CATG 4 cut(s) 153, 244, 287, 398
NmeAIII GCCGAG 2 cut(s) 225, 415
NmuCI GTSAC 1 cut(s) 556
NspI RCATGY 1 cut(s) 244
NspV TTCGAA 1 cut(s) 69
OliI CACNNNNGTG 1 cut(s) 44
PagI TCATGA 1 cut(s) 283
PceI AGGCCT 1 cut(s) 290
PctI GAATGC 2 cut(s) 428, 521
PfeI GAWTC 3 cut(s) 399, 510, 542
PkrI GCNGC 3 cut(s) 128, 131, 214
PleI GAGTC 3 cut(s) 179, 260, 483
PpsI GAGTC 3 cut(s) 179, 260, 483
Psp6I CCWGG 2 cut(s) 437, 593
PspFI CCCAGC 1 cut(s) 460
PspGI CCWGG 2 cut(s) 437, 593
PvuII CAGCTG 1 cut(s) 212
RsaI GTAC 4 cut(s) 18, 257, 383, 488
RsaNI GTAC 4 cut(s) 17, 256, 382, 487
RseI CAYNNNNRTG 1 cut(s) 44
SatI GCNGC 3 cut(s) 127, 130, 213
ScaI AGTACT 1 cut(s) 18
SchI GAGTC 3 cut(s) 180, 260, 484
ScrFI CCNGG 2 cut(s) 439, 595
SduI GDGCHC 1 cut(s) 237
SetI ASST 9 cut(s) 64, 214, 231, 318, 414, 466, 486, 519, 596
SfuI TTCGAA 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 44
Sse9I AATT 1 cut(s) 418
SseBI AGGCCT 1 cut(s) 290
SsiI CCGC 1 cut(s) 126
SspMI CTAG 1 cut(s) 35
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 2 cut(s) 437, 593
StyI CCWWGG 1 cut(s) 58
TaiI ACGT 1 cut(s) 231
TaqI TCGA 3 cut(s) 69, 174, 354
TasI AATT 1 cut(s) 418
TatI WGTACW 3 cut(s) 16, 255, 486
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 3 cut(s) 399, 510, 542
TscAI CASTG 1 cut(s) 439
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 2 cut(s) 129, 212
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 4 cut(s) 17, 54, 138, 514
TspRI CASTG 1 cut(s) 439
XceI RCATGY 1 cut(s) 244
XspI CTAG 1 cut(s) 35
ZrmI AGTACT 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.