RchiOBHm_Chr2g0171201

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017420
Physical Location & Seq
Forward (+)
84857452 .. 84857955
504 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53863

Sequence Viewer

Length: 504 bp
ATGTTTAGAAATGTTGATTGCAGTTTTCTTGTTCATTTTTGTAGCCGTTGGCAACCCCCTGCTGTAGGATCTCTGAAGATAAATTTTCACTCTGCATTTTCCAAAAGCAGTGGCGTAGGCATTGGCGGACTATGTTCTCTGGTGCGCAATGATCGAGGAGAGCTTTTGGACGGTTTGACTACCAGGATGGTTGCTGGATTTCCTTTTGATGCAGAAGTGAAGGCCGCAAAGCTTGTGTGTTCTCTTGGTGTGGATTATAAGGGAGTGAAGATTGAGTTTGAGGGTGGAAGCAAGTTGCTGACTAACTTGGTTGTTGGAAAAACTCTTCTGGGTTCCAAGTGGACCGCAAGAAGCTTAATGGATGAGATAAAGCTTAGTTTGGGGGAGCTTAGGGCTGGCTATTCCTGGACCCATTGTGCCAAGACAGGCAATATGGCGGCATCCTATTTCTCGAGCAGGTTGTGTGAAAATGAATTCATTGAAGGAATGCAGTGGGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

167

Amino Acids

18.21

Weight (kDa)

8.77

Isoelectric Point (pI)

20.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
Acc16I TGCGCA 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 447
AciI CCGC 4 cut(s) 126, 225, 345, 437
AclWI GGATC 1 cut(s) 76
AcsI RAATTY 2 cut(s) 82, 473
AcuI CTGAAG 1 cut(s) 95
AfiI CCNNNNNNNGG 1 cut(s) 65
AgsI TTSAA 1 cut(s) 482
AjnI CCWGG 2 cut(s) 182, 404
AluBI AGCT 5 cut(s) 163, 232, 354, 373, 388
AluI AGCT 5 cut(s) 163, 232, 354, 373, 388
AlwI GGATC 1 cut(s) 76
Ama87I CYCGRG 1 cut(s) 451
AoxI GGCC 1 cut(s) 222
ApoI RAATTY 2 cut(s) 82, 473
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 2 cut(s) 342, 408
AvaI CYCGRG 1 cut(s) 451
AvaII GGWCC 2 cut(s) 342, 408
BccI CCATC 1 cut(s) 181
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 2 cut(s) 184, 406
BfmI CTRYAG 1 cut(s) 63
BfuAI ACCTGC 1 cut(s) 447
BisI GCNGC 2 cut(s) 225, 438
BlsI GCNGC 2 cut(s) 226, 439
Bme1390I CCNGG 2 cut(s) 184, 406
Bme18I GGWCC 2 cut(s) 342, 408
BmeT110I CYCGRG 1 cut(s) 451
BmgT120I GGNCC 2 cut(s) 342, 408
BmiI GGNNCC 2 cut(s) 334, 410
BmrFI CCNGG 2 cut(s) 184, 406
BmsI GCATC 2 cut(s) 199, 449
Bpu10I CCTNAGC 1 cut(s) 389
Bsc4I CCNNNNNNNGG 1 cut(s) 65
Bse3DI GCAATG 1 cut(s) 154
BseBI CCWGG 2 cut(s) 184, 406
BseGI GGATG 3 cut(s) 192, 367, 440
BseLI CCNNNNNNNGG 1 cut(s) 65
BseMI GCAATG 1 cut(s) 154
BseRI GAGGAG 1 cut(s) 171
BshFI GGCC 1 cut(s) 224
BsiHKCI CYCGRG 1 cut(s) 451
BslI CCNNNNNNNGG 1 cut(s) 65
BsmI GAATGC 1 cut(s) 492
BsnI GGCC 1 cut(s) 224
BsoBI CYCGRG 1 cut(s) 451
Bsp143I GATC 2 cut(s) 68, 151
BspACI CCGC 4 cut(s) 126, 225, 345, 437
BspANI GGCC 1 cut(s) 224
BspLI GGNNCC 2 cut(s) 334, 410
BspMI ACCTGC 1 cut(s) 447
BspPI GGATC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 154
BssMI GATC 2 cut(s) 68, 151
Bst2UI CCWGG 2 cut(s) 184, 406
Bst4CI ACNGT 1 cut(s) 173
Bst6I CTCTTC 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 2 cut(s) 374, 389
BstENI CCTNNNNNAGG 1 cut(s) 63
BstF5I GGATG 3 cut(s) 192, 367, 440
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 2 cut(s) 71, 154
BstMBI GATC 2 cut(s) 68, 151
BstNI CCWGG 2 cut(s) 184, 406
BstSCI CCNGG 2 cut(s) 182, 404
BstSFI CTRYAG 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BsuRI GGCC 1 cut(s) 224
BtsCI GGATG 3 cut(s) 192, 367, 440
BtsI GCAGTG 2 cut(s) 115, 497
BtsIMutI CAGTG 2 cut(s) 115, 497
BveI ACCTGC 1 cut(s) 447
Cac8I GCNNGC 1 cut(s) 397
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 2 cut(s) 342, 408
CspCI CAANNNNNGTGG 2 cut(s) 91, 126
CviJI RGCY 9 cut(s) 45, 163, 224, 232, 354, 373, 388, 395, 399
CviKI_1 RGCY 9 cut(s) 45, 163, 224, 232, 354, 373, 388, 395, 399
DdeI CTNAG 2 cut(s) 374, 389
DpnI GATC 2 cut(s) 70, 153
DpnII GATC 2 cut(s) 68, 151
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 1 cut(s) 141
Eco47I GGWCC 2 cut(s) 342, 408
Eco57I CTGAAG 1 cut(s) 95
Eco88I CYCGRG 1 cut(s) 451
EcoNI CCTNNNNNAGG 1 cut(s) 63
EcoRI GAATTC 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 182, 404
FaiI YATR 3 cut(s) 133, 258, 434
Fnu4HI GCNGC 2 cut(s) 225, 438
FokI GGATG 3 cut(s) 199, 374, 427
Fsp4HI GCNGC 2 cut(s) 225, 438
FspI TGCGCA 1 cut(s) 146
GlaI GCGC 1 cut(s) 146
GluI GCNGC 2 cut(s) 225, 438
HaeIII GGCC 1 cut(s) 224
HhaI GCGC 1 cut(s) 147
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HindIII AAGCTT 3 cut(s) 230, 352, 371
Hpy166II GTNNAC 1 cut(s) 342
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 1 cut(s) 451
Hpy8I GTNNAC 1 cut(s) 342
HpyAV CCTTC 2 cut(s) 214, 476
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 4 cut(s) 21, 95, 212, 490
HpyF3I CTNAG 2 cut(s) 374, 389
HspAI GCGC 1 cut(s) 145
Kzo9I GATC 2 cut(s) 68, 151
LmnI GCTCC 1 cut(s) 385
LweI GCATC 2 cut(s) 199, 449
MalI GATC 2 cut(s) 70, 153
MboI GATC 2 cut(s) 68, 151
MboII GAAGA 3 cut(s) 88, 280, 317
MflI RGATCY 1 cut(s) 68
MluCI AATT 2 cut(s) 82, 473
MmeI TCCRAC 2 cut(s) 295, 478
MnlI CCTC 2 cut(s) 149, 274
MseI TTAA 1 cut(s) 356
MspR9I CCNGG 2 cut(s) 184, 406
Mva1269I GAATGC 1 cut(s) 492
MvaI CCWGG 2 cut(s) 184, 406
NdeII GATC 2 cut(s) 68, 151
NlaIV GGNNCC 2 cut(s) 334, 410
NsbI TGCGCA 1 cut(s) 146
PaeR7I CTCGAG 1 cut(s) 451
PctI GAATGC 1 cut(s) 492
PfoI TCCNGGA 1 cut(s) 404
PkrI GCNGC 2 cut(s) 226, 439
PsiI TTATAA 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 182, 404
PspGI CCWGG 2 cut(s) 182, 404
PspN4I GGNNCC 2 cut(s) 334, 410
PspPI GGNCC 2 cut(s) 342, 408
PsuI RGATCY 1 cut(s) 68
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 2 cut(s) 225, 438
Sau3AI GATC 2 cut(s) 68, 151
Sau96I GGNCC 2 cut(s) 342, 408
ScrFI CCNGG 2 cut(s) 184, 406
SetI ASST 6 cut(s) 165, 234, 356, 375, 390, 461
SfaNI GCATC 2 cut(s) 199, 449
SfcI CTRYAG 1 cut(s) 63
Sfr274I CTCGAG 1 cut(s) 451
SinI GGWCC 2 cut(s) 342, 408
SlaI CTCGAG 1 cut(s) 451
SmlI CTYRAG 1 cut(s) 451
SmoI CTYRAG 1 cut(s) 451
Sse9I AATT 2 cut(s) 82, 473
SsiI CCGC 4 cut(s) 126, 225, 345, 437
StyD4I CCNGG 2 cut(s) 182, 404
TaaI ACNGT 1 cut(s) 173
TaqI TCGA 2 cut(s) 154, 452
TasI AATT 2 cut(s) 82, 473
TauI GCSGC 2 cut(s) 227, 440
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TscAI CASTG 2 cut(s) 115, 497
TspDTI ATGAA 3 cut(s) 23, 466, 486
TspRI CASTG 2 cut(s) 115, 497
VpaK11BI GGWCC 2 cut(s) 342, 408
XagI CCTNNNNNAGG 1 cut(s) 63
XapI RAATTY 2 cut(s) 82, 473
XhoI CTCGAG 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.