Rroxscaffold_5G00352740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017420
Physical Location & Seq
Reverse (-)
30008811 .. 30013240
4430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00352740.1

Sequence Viewer

Length: 855 bp
ATGTTCACGCTTCCCGCATCATTTGATATCAAAGCGGGTATTGCCCGCTCCTTAGCGTACGACGATCCAAGGGAGAAGTTCACTACCACCAACCTCAACGACGCTGGTTGTAGAGTATCTATCAAAGAAAATTTAGAGTTAGGTGCTGAAATTTTCGCTGGAGGTGCAGTATCACTGCTTGTTAACGATAGATTCTGGAGTCCTCATGCATTCCAGGTTAAGTTGAATTGCAGCACCTTCTGGGTCAAGTCAAGTTGTAAGGTTGGATTATGTGTCATTGCACGCGATGCTGATCGTTCTCTAGTCTTTTCGAATGTGTGCTTGGGGTGGCCACATCGGCACCTGATGCAGAAGCAAAAGCGTGCCTCATGGCTTTACGACTTGCCACGGAACAACTTGCAGGCCGACCATTTGTGCTTGAGTCTCGTTTTTCCGAACGCCCTTCACGACGTTCGAAGATTTTGTTCTTTCTTAGTATATCGAGACCGGGATGTTGATGTTGATTCTAGTACCGTTGTACTTTATGCCGCTGTTTCTTCCGAATTGAGCCTTTGGCAACCCCTTGCTAAAGGATCTATCAAGATAAATTTTCATTCTGCATTTTCTAAAAGGAGTGGTGTAGGAATTGGTGGACTCTCGTTCTCCGTGAAGGCTGCGAAGCTTGCTTGTTCTGTTGCCTTGGATTATAAGAGTTTGAAGATCGAGTTCGAAGGTGGAAGTGAATTATTGACTAACCAAGTTGTTGGAAGAACGCTTCGGATTCAAAGTGGACCGCAAGAAGGAGGGCAGTGGATGGTTGATCTTGAAGAGATTTTGAAGGAAGATATGAAGGCTGTTTCCCATCCGCTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

284

Amino Acids

31.52

Weight (kDa)

8.38

Isoelectric Point (pI)

38.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 687
AccB1I GGYRCC 1 cut(s) 339
AccBSI CCGCTC 1 cut(s) 48
AccII CGCG 1 cut(s) 285
AciI CCGC 6 cut(s) 15, 35, 46, 528, 773, 845
AclWI GGATC 2 cut(s) 59, 580
AcoI YGGCCR 1 cut(s) 329
AcsI RAATTY 3 cut(s) 130, 150, 586
AfaI GTAC 3 cut(s) 59, 511, 519
AfiI CCNNNNNNNGG 1 cut(s) 779
AgsI TTSAA 5 cut(s) 226, 697, 764, 806, 817
AjnI CCWGG 1 cut(s) 213
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 1 cut(s) 661
AluI AGCT 1 cut(s) 661
Alw26I GTCTC 2 cut(s) 428, 477
AlwI GGATC 2 cut(s) 59, 580
AoxI GGCC 2 cut(s) 329, 402
ApeKI GCWGC 2 cut(s) 231, 653
ApoI RAATTY 3 cut(s) 130, 150, 586
AspS9I GGNCC 1 cut(s) 770
AsuC2I CCSGG 1 cut(s) 488
AsuII TTCGAA 3 cut(s) 311, 454, 708
AvaII GGWCC 1 cut(s) 770
BalI TGGCCA 1 cut(s) 331
BanI GGYRCC 1 cut(s) 339
BbvI GCAGC 2 cut(s) 243, 640
BccI CCATC 2 cut(s) 787, 849
BciT130I CCWGG 1 cut(s) 215
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 2 cut(s) 428, 477
BfaI CTAG 2 cut(s) 302, 507
BglI GCCNNNNNGGC 1 cut(s) 337
BisI GCNGC 3 cut(s) 232, 528, 654
BlsI GCNGC 3 cut(s) 233, 529, 655
Bme1390I CCNGG 2 cut(s) 215, 488
Bme18I GGWCC 1 cut(s) 770
BmgT120I GGNCC 1 cut(s) 770
BmiI GGNNCC 1 cut(s) 341
BmrFI CCNGG 2 cut(s) 215, 488
BmsI GCATC 3 cut(s) 26, 277, 336
BpmI CTGGAG 2 cut(s) 180, 217
Bpu10I CCTNAGC 1 cut(s) 52
Bpu14I TTCGAA 3 cut(s) 311, 454, 708
BpuEI CTTGAG 1 cut(s) 439
BpuMI CCSGG 1 cut(s) 488
BsaBI GATNNNNATC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 477
BsaJI CCNNGG 3 cut(s) 68, 386, 678
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 1 cut(s) 779
Bse3DI GCAATG 1 cut(s) 276
Bse8I GATNNNNATC 1 cut(s) 291
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 3 cut(s) 68, 386, 678
BseGI GGATG 3 cut(s) 496, 798, 841
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 1 cut(s) 779
BseMI GCAATG 1 cut(s) 276
BseXI GCAGC 2 cut(s) 243, 640
BsgI GTGCAG 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 285
BshFI GGCC 2 cut(s) 331, 404
BshNI GGYRCC 1 cut(s) 339
BsiSI CCGG 1 cut(s) 487
BsiWI CGTACG 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 779
BsmAI GTCTC 2 cut(s) 428, 477
BsmI GAATGC 1 cut(s) 209
BsnI GGCC 2 cut(s) 331, 404
Bso31I GGTCTC 1 cut(s) 477
Bsp119I TTCGAA 3 cut(s) 311, 454, 708
Bsp143I GATC 5 cut(s) 64, 292, 572, 699, 799
BspACI CCGC 6 cut(s) 15, 35, 46, 528, 773, 845
BspANI GGCC 2 cut(s) 331, 404
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 341
BspPI GGATC 2 cut(s) 59, 580
BspT104I TTCGAA 3 cut(s) 311, 454, 708
BspT107I GGYRCC 1 cut(s) 339
BspTNI GGTCTC 1 cut(s) 477
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 1 cut(s) 276
BssECI CCNNGG 3 cut(s) 68, 386, 678
BssMI GATC 5 cut(s) 64, 292, 572, 699, 799
BssT1I CCWWGG 2 cut(s) 68, 678
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 514
Bst6I CTCTTC 1 cut(s) 801
BstAPI GCANNNNNTGC 2 cut(s) 287, 346
BstBI TTCGAA 3 cut(s) 311, 454, 708
BstC8I GCNNGC 5 cut(s) 46, 283, 363, 402, 663
BstDEI CTNAG 2 cut(s) 52, 472
BstDSI CCRYGG 1 cut(s) 386
BstF5I GGATG 3 cut(s) 496, 798, 841
BstFNI CGCG 1 cut(s) 285
BstKTI GATC 5 cut(s) 67, 295, 575, 702, 802
BstMAI GTCTC 2 cut(s) 428, 477
BstMBI GATC 5 cut(s) 64, 292, 572, 699, 799
BstMWI GCNNNNNNNGC 6 cut(s) 41, 164, 287, 337, 346, 662
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 2 cut(s) 213, 486
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 2 cut(s) 243, 640
BstX2I RGATCY 1 cut(s) 572
BstXI CCANNNNNNTGG 1 cut(s) 743
BstYI RGATCY 1 cut(s) 572
BsuRI GGCC 2 cut(s) 331, 404
BtgI CCRYGG 1 cut(s) 386
BtgZI GCGATG 1 cut(s) 300
BtsCI GGATG 3 cut(s) 496, 798, 841
BtsI GCAGTG 2 cut(s) 173, 794
BtsIMutI CAGTG 2 cut(s) 173, 794
Cac8I GCNNGC 5 cut(s) 46, 283, 363, 402, 663
Cfr13I GGNCC 1 cut(s) 770
CseI GACGC 1 cut(s) 110
Csp6I GTAC 3 cut(s) 58, 510, 518
CviAII CATG 2 cut(s) 206, 369
CviJI RGCY 7 cut(s) 331, 373, 404, 549, 653, 661, 833
CviKI_1 RGCY 7 cut(s) 331, 373, 404, 549, 653, 661, 833
CviQI GTAC 3 cut(s) 58, 510, 518
DdeI CTNAG 2 cut(s) 52, 472
DpnI GATC 5 cut(s) 66, 294, 574, 701, 801
DpnII GATC 5 cut(s) 64, 292, 572, 699, 799
EaeI YGGCCR 1 cut(s) 329
Eam1104I CTCTTC 1 cut(s) 801
EarI CTCTTC 1 cut(s) 801
Eco130I CCWWGG 2 cut(s) 68, 678
Eco31I GGTCTC 1 cut(s) 477
Eco32I GATATC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 770
EcoRII CCWGG 1 cut(s) 213
EcoRV GATATC 1 cut(s) 28
EcoT14I CCWWGG 2 cut(s) 68, 678
EcoT22I ATGCAT 1 cut(s) 211
ErhI CCWWGG 2 cut(s) 68, 678
FaeI CATG 2 cut(s) 209, 372
FaiI YATR 7 cut(s) 207, 271, 370, 478, 525, 687, 827
FatI CATG 2 cut(s) 205, 368
FauI CCCGC 3 cut(s) 22, 28, 53
Fnu4HI GCNGC 3 cut(s) 232, 528, 654
FokI GGATG 3 cut(s) 503, 805, 828
Fsp4HI GCNGC 3 cut(s) 232, 528, 654
FspBI CTAG 2 cut(s) 302, 507
GluI GCNGC 3 cut(s) 232, 528, 654
GsuI CTGGAG 2 cut(s) 180, 217
HaeIII GGCC 2 cut(s) 331, 404
HapII CCGG 1 cut(s) 487
HgaI GACGC 1 cut(s) 110
Hin1II CATG 2 cut(s) 209, 372
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HindIII AAGCTT 1 cut(s) 659
HinfI GANTC 6 cut(s) 192, 199, 421, 503, 633, 760
HpaI GTTAAC 1 cut(s) 184
HpaII CCGG 1 cut(s) 487
Hpy166II GTNNAC 5 cut(s) 6, 81, 184, 632, 770
Hpy188I TCNGA 3 cut(s) 435, 541, 759
Hpy188III TCNNGA 5 cut(s) 196, 446, 482, 580, 803
Hpy8I GTNNAC 5 cut(s) 6, 81, 184, 632, 770
Hpy99I CGWCG 3 cut(s) 65, 104, 452
HpyAV CCTTC 7 cut(s) 247, 452, 643, 704, 773, 811, 823
HpyCH4III ACNGT 1 cut(s) 514
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 7 cut(s) 167, 209, 231, 281, 349, 400, 599
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 164, 287, 337, 346, 662
HpyF3I CTNAG 2 cut(s) 52, 472
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 2 cut(s) 209, 372
KspAI GTTAAC 1 cut(s) 184
Kzo9I GATC 5 cut(s) 64, 292, 572, 699, 799
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 9 cut(s) 90, 144, 181, 200, 226, 227, 356, 386, 500
Lsp1109I GCAGC 2 cut(s) 243, 640
LweI GCATC 3 cut(s) 26, 277, 336
MaeI CTAG 2 cut(s) 302, 507
MaeII ACGT 1 cut(s) 450
MalI GATC 5 cut(s) 66, 294, 574, 701, 801
MbiI CCGCTC 1 cut(s) 48
MboI GATC 5 cut(s) 64, 292, 572, 699, 799
MboII GAAGA 6 cut(s) 468, 528, 709, 759, 818, 833
MflI RGATCY 1 cut(s) 572
MlsI TGGCCA 1 cut(s) 331
MluCI AATT 7 cut(s) 130, 150, 226, 542, 586, 624, 722
MluNI TGGCCA 1 cut(s) 331
MlyI GAGTC 3 cut(s) 208, 430, 627
MmeI TCCRAC 2 cut(s) 244, 724
MnlI CCTC 5 cut(s) 104, 155, 213, 376, 776
Mox20I TGGCCA 1 cut(s) 331
Mph1103I ATGCAT 1 cut(s) 211
MscI TGGCCA 1 cut(s) 331
MseI TTAA 2 cut(s) 183, 219
Msp20I TGGCCA 1 cut(s) 331
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 1 cut(s) 487
MspR9I CCNGG 2 cut(s) 215, 488
Mva1269I GAATGC 1 cut(s) 209
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 6 cut(s) 41, 164, 287, 337, 346, 662
NciI CCSGG 1 cut(s) 488
NdeII GATC 5 cut(s) 64, 292, 572, 699, 799
NlaIII CATG 2 cut(s) 209, 372
NlaIV GGNNCC 1 cut(s) 341
NsiI ATGCAT 1 cut(s) 211
NspV TTCGAA 3 cut(s) 311, 454, 708
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PctI GAATGC 1 cut(s) 209
PfeI GAWTC 3 cut(s) 192, 503, 760
Pfl23II CGTACG 1 cut(s) 57
PkrI GCNGC 3 cut(s) 233, 529, 655
PleI GAGTC 3 cut(s) 207, 429, 627
PpsI GAGTC 3 cut(s) 207, 429, 627
PsiI TTATAA 1 cut(s) 687
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspLI CGTACG 1 cut(s) 57
PspN4I GGNNCC 1 cut(s) 341
PspPI GGNCC 1 cut(s) 770
PsuI RGATCY 1 cut(s) 572
RsaI GTAC 3 cut(s) 59, 511, 519
RsaNI GTAC 3 cut(s) 58, 510, 518
SaqAI TTAA 2 cut(s) 183, 219
SatI GCNGC 3 cut(s) 232, 528, 654
Sau3AI GATC 5 cut(s) 64, 292, 572, 699, 799
Sau96I GGNCC 1 cut(s) 770
SchI GAGTC 3 cut(s) 208, 430, 627
ScrFI CCNGG 2 cut(s) 215, 488
SfaNI GCATC 3 cut(s) 26, 277, 336
SfuI TTCGAA 3 cut(s) 311, 454, 708
SinI GGWCC 1 cut(s) 770
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
Sse9I AATT 7 cut(s) 130, 150, 226, 542, 586, 624, 722
SsiI CCGC 6 cut(s) 15, 35, 46, 528, 773, 845
SspMI CTAG 2 cut(s) 302, 507
StyD4I CCNGG 2 cut(s) 213, 486
StyI CCWWGG 2 cut(s) 68, 678
TaaI ACNGT 1 cut(s) 514
TaiI ACGT 1 cut(s) 453
TaqI TCGA 5 cut(s) 311, 454, 481, 702, 708
TasI AATT 7 cut(s) 130, 150, 226, 542, 586, 624, 722
TatI WGTACW 1 cut(s) 517
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 3 cut(s) 192, 503, 760
Tru1I TTAA 2 cut(s) 183, 219
Tru9I TTAA 2 cut(s) 183, 219
TscAI CASTG 2 cut(s) 180, 794
TseI GCWGC 2 cut(s) 231, 653
TspDTI ATGAA 2 cut(s) 581, 842
TspGWI ACGGA 2 cut(s) 403, 634
TspRI CASTG 2 cut(s) 180, 794
VpaK11BI GGWCC 1 cut(s) 770
XapI RAATTY 3 cut(s) 130, 150, 586
XspI CTAG 2 cut(s) 302, 507
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.