Rw2G051960

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017420
Physical Location & Seq
Forward (+)
79771414 .. 79773290
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G051960.1

Sequence Viewer

Length: 639 bp
ATGAGTGCCGTAAACTTTAGATCTAGTATATTTTATGATTTTCAGAATGTCTCTTATGCATTTGCTGATCAGGATGTTGATGCTAATTCTGTTGTACTCACTGCTGCTCTTGCTTCCAAATTGAGTTTTCTTGTTCATTTTTGTAGCCGTTGGCAACCCCCTGCTGTAGGATCTCTGAAGATAAATTTTCACTCTGCATTTTCCAAAAGCAGTGGCGTAGGCATTGGCGGACTATGTTCTCTGGTGCGCAATGATTGGGGAGAGCTTTTGGACGGTTTGACTACCAGGGTGGTTGCTGGATTTCCTTTTGATGCAGAAGTGAAGGCCGCAAAGCTTGTGTGTTCTCTTGGTGTGGATTATAAGGGAGTGAAGATTGAGTTTGAGGGTGGAAGCAAGTTGCTGACTAACTTGGTTGTTGGAAAAACTCTTCTGGGTTCCAAGTGGACTGCAAGAAGCTTAATGGATGAGATAAAGCTTAGTTTGGGGGAGCTTAGGGCTGGCTATTCCTGGACCCATTGTGCGAAGACAGGCAATATGGCGGCATCCTATTTCTCGAGCAGGTTGTGTGAAAATGAATTCATTGAAGGAATGCAGTGGTTGGATGATCTCCAGGAGATTTTGGTGGGAGATATGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

212

Amino Acids

23.1

Weight (kDa)

5.62

Isoelectric Point (pI)

31.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
Acc16I TGCGCA 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 549
AciI CCGC 3 cut(s) 228, 327, 539
AclWI GGATC 1 cut(s) 178
AcsI RAATTY 2 cut(s) 184, 575
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 1 cut(s) 96
AfiI CCNNNNNNNGG 1 cut(s) 167
AgsI TTSAA 1 cut(s) 584
AjnI CCWGG 3 cut(s) 284, 506, 609
AluBI AGCT 5 cut(s) 265, 334, 456, 475, 490
AluI AGCT 5 cut(s) 265, 334, 456, 475, 490
Alw26I GTCTC 1 cut(s) 55
AlwI GGATC 1 cut(s) 178
Ama87I CYCGRG 1 cut(s) 553
AoxI GGCC 1 cut(s) 324
ApeKI GCWGC 1 cut(s) 104
ApoI RAATTY 2 cut(s) 184, 575
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 1 cut(s) 510
AvaI CYCGRG 1 cut(s) 553
AvaII GGWCC 1 cut(s) 510
BbsI GAAGAC 1 cut(s) 530
BbvI GCAGC 1 cut(s) 91
BceAI ACGGC 1 cut(s) 132
BciT130I CCWGG 3 cut(s) 286, 508, 611
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 2 cut(s) 165, 635
BfuAI ACCTGC 1 cut(s) 549
BglII AGATCT 1 cut(s) 20
BisI GCNGC 3 cut(s) 105, 327, 540
BlsI GCNGC 3 cut(s) 106, 328, 541
Bme1390I CCNGG 3 cut(s) 286, 508, 611
Bme18I GGWCC 1 cut(s) 510
BmeT110I CYCGRG 1 cut(s) 553
BmgT120I GGNCC 1 cut(s) 510
BmiI GGNNCC 2 cut(s) 436, 512
BmrFI CCNGG 3 cut(s) 286, 508, 611
BmsI GCATC 3 cut(s) 70, 301, 551
BpiI GAAGAC 1 cut(s) 530
BpmI CTGGAG 1 cut(s) 593
Bpu10I CCTNAGC 1 cut(s) 491
BsaJI CCNNGG 1 cut(s) 285
Bsc4I CCNNNNNNNGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 256
BseBI CCWGG 3 cut(s) 286, 508, 611
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 4 cut(s) 79, 469, 542, 607
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMI GCAATG 1 cut(s) 256
BseXI GCAGC 1 cut(s) 91
BshFI GGCC 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 553
BslI CCNNNNNNNGG 1 cut(s) 167
BsmAI GTCTC 1 cut(s) 55
BsmI GAATGC 1 cut(s) 594
BsnI GGCC 1 cut(s) 326
BsoBI CYCGRG 1 cut(s) 553
Bsp143I GATC 4 cut(s) 20, 67, 170, 604
BspACI CCGC 3 cut(s) 228, 327, 539
BspANI GGCC 1 cut(s) 326
BspLI GGNNCC 2 cut(s) 436, 512
BspMI ACCTGC 1 cut(s) 549
BspPI GGATC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 4 cut(s) 20, 67, 170, 604
Bst2UI CCWGG 3 cut(s) 286, 508, 611
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 1 cut(s) 432
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 2 cut(s) 476, 491
BstENI CCTNNNNNAGG 1 cut(s) 165
BstF5I GGATG 4 cut(s) 79, 469, 542, 607
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 4 cut(s) 23, 70, 173, 607
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 4 cut(s) 20, 67, 170, 604
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNI CCWGG 3 cut(s) 286, 508, 611
BstSCI CCNGG 3 cut(s) 284, 506, 609
BstSFI CTRYAG 2 cut(s) 165, 635
BstV1I GCAGC 1 cut(s) 91
BstV2I GAAGAC 1 cut(s) 530
BstX2I RGATCY 2 cut(s) 20, 170
BstYI RGATCY 2 cut(s) 20, 170
BsuRI GGCC 1 cut(s) 326
BtsCI GGATG 4 cut(s) 79, 469, 542, 607
BtsI GCAGTG 3 cut(s) 99, 217, 599
BtsIMutI CAGTG 3 cut(s) 99, 217, 599
BveI ACCTGC 1 cut(s) 549
Cac8I GCNNGC 1 cut(s) 499
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 510
Csp6I GTAC 1 cut(s) 95
CspCI CAANNNNNGTGG 2 cut(s) 193, 228
CviJI RGCY 9 cut(s) 147, 265, 326, 334, 456, 475, 490, 497, 501
CviKI_1 RGCY 9 cut(s) 147, 265, 326, 334, 456, 475, 490, 497, 501
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 2 cut(s) 476, 491
DpnI GATC 4 cut(s) 22, 69, 172, 606
DpnII GATC 4 cut(s) 20, 67, 170, 604
Eam1104I CTCTTC 1 cut(s) 432
EarI CTCTTC 1 cut(s) 432
EciI GGCGGA 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 510
Eco57I CTGAAG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 553
EcoNI CCTNNNNNAGG 1 cut(s) 165
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 3 cut(s) 284, 506, 609
EcoT22I ATGCAT 1 cut(s) 61
FaiI YATR 7 cut(s) 29, 36, 57, 235, 360, 536, 632
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 3 cut(s) 105, 327, 540
FokI GGATG 4 cut(s) 86, 476, 529, 614
Fsp4HI GCNGC 3 cut(s) 105, 327, 540
FspBI CTAG 1 cut(s) 24
FspI TGCGCA 1 cut(s) 248
GlaI GCGC 1 cut(s) 248
GluI GCNGC 3 cut(s) 105, 327, 540
GsuI CTGGAG 1 cut(s) 593
HaeIII GGCC 1 cut(s) 326
HhaI GCGC 1 cut(s) 249
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HindIII AAGCTT 3 cut(s) 332, 454, 473
Hpy166II GTNNAC 2 cut(s) 13, 444
Hpy188I TCNGA 2 cut(s) 45, 177
Hpy188III TCNNGA 2 cut(s) 71, 553
Hpy8I GTNNAC 2 cut(s) 13, 444
HpyAV CCTTC 2 cut(s) 316, 578
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4V TGCA 5 cut(s) 59, 197, 314, 449, 592
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 2 cut(s) 476, 491
HspAI GCGC 1 cut(s) 247
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 4 cut(s) 20, 67, 170, 604
LmnI GCTCC 1 cut(s) 487
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 3 cut(s) 70, 301, 551
MaeI CTAG 1 cut(s) 24
MalI GATC 4 cut(s) 22, 69, 172, 606
MboI GATC 4 cut(s) 20, 67, 170, 604
MboII GAAGA 4 cut(s) 190, 382, 419, 535
MflI RGATCY 2 cut(s) 20, 170
MluCI AATT 4 cut(s) 85, 119, 184, 575
MmeI TCCRAC 2 cut(s) 397, 579
MnlI CCTC 1 cut(s) 376
Mph1103I ATGCAT 1 cut(s) 61
MseI TTAA 1 cut(s) 458
MspR9I CCNGG 3 cut(s) 286, 508, 611
Mva1269I GAATGC 1 cut(s) 594
MvaI CCWGG 3 cut(s) 286, 508, 611
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeII GATC 4 cut(s) 20, 67, 170, 604
NlaIV GGNNCC 2 cut(s) 436, 512
NsbI TGCGCA 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 61
PaeR7I CTCGAG 1 cut(s) 553
PctI GAATGC 1 cut(s) 594
PfoI TCCNGGA 2 cut(s) 506, 609
PkrI GCNGC 3 cut(s) 106, 328, 541
PsiI TTATAA 1 cut(s) 360
Psp6I CCWGG 3 cut(s) 284, 506, 609
PspGI CCWGG 3 cut(s) 284, 506, 609
PspN4I GGNNCC 2 cut(s) 436, 512
PspPI GGNCC 1 cut(s) 510
PsuI RGATCY 2 cut(s) 20, 170
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
SaqAI TTAA 1 cut(s) 458
SatI GCNGC 3 cut(s) 105, 327, 540
Sau3AI GATC 4 cut(s) 20, 67, 170, 604
Sau96I GGNCC 1 cut(s) 510
ScrFI CCNGG 3 cut(s) 286, 508, 611
SetI ASST 6 cut(s) 267, 336, 458, 477, 492, 563
SfaNI GCATC 3 cut(s) 70, 301, 551
SfcI CTRYAG 2 cut(s) 165, 635
Sfr274I CTCGAG 1 cut(s) 553
SinI GGWCC 1 cut(s) 510
SlaI CTCGAG 1 cut(s) 553
SmlI CTYRAG 1 cut(s) 553
SmoI CTYRAG 1 cut(s) 553
Sse9I AATT 4 cut(s) 85, 119, 184, 575
SsiI CCGC 3 cut(s) 228, 327, 539
SspMI CTAG 1 cut(s) 24
StyD4I CCNGG 3 cut(s) 284, 506, 609
TaaI ACNGT 1 cut(s) 275
TaqI TCGA 1 cut(s) 554
TasI AATT 4 cut(s) 85, 119, 184, 575
TatI WGTACW 1 cut(s) 94
TauI GCSGC 2 cut(s) 329, 542
Tru1I TTAA 1 cut(s) 458
Tru9I TTAA 1 cut(s) 458
TscAI CASTG 3 cut(s) 106, 217, 599
TseI GCWGC 1 cut(s) 104
TspDTI ATGAA 3 cut(s) 125, 568, 588
TspRI CASTG 3 cut(s) 106, 217, 599
VpaK11BI GGWCC 1 cut(s) 510
XagI CCTNNNNNAGG 1 cut(s) 165
XapI RAATTY 2 cut(s) 184, 575
XhoI CTCGAG 1 cut(s) 553
XspI CTAG 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.