RchiOBHm_Chr2g0176161

Glycerophosphodiester phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011688
Physical Location & Seq
Forward (+)
88086808 .. 88089439
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54318

Sequence Viewer

Length: 1296 bp
ATGTCCTTGAGAAATATAAATAGCCCTCCTCAACACTCTTGGATTTCAACAAAAACAACAACACCAAGGTCCCCTCGTTTGTGTTCCCCTCGTCCTTTTTATTTTCCCGTCTTCAAAAAAAAAAAAAAAAAAAAGGATACTATGGCTCTTAAGGCTGTCCATGTCTCCGACGTCCCTAACCTCGATCAGGTCCCGGAAAATGCCTCTCTTCAGCTGTACTCCACCCGCTTCTCCAAAGGTGTGGAATTTGGTCGGGTGGCATCGAGAATACCTAAGTTTCTAGTGATCGGACACAGAGGGAATGGAATGAACGCTTTACAGTCGTCTGATCGGAGAATGAGAGCCATTAAGGAAAACTCCATCATGTCGTTCAACACTGCTGCCAAATACCCTCTTGATTTTGTGGAATTTGACGTTCAGGTAACCAAAGATGGCTGCCCCGTCATTTTCCACGACGACTTCATCCTCACCGAAGATCAGGGTACTGTGTTTGAGAAGAGAATACCTGAACTGTGTTTGACGGAGTTCCTAAATTATGGTCCACAAAGAGAGGCAGCAAAGGATGGAAAGCCTTTGTTAAGGAAAAACAAAGACGGTCAAATTGTAAGATGGGAGGTTGAAAAAGATGATTCCCTCTGTACCCTCCAAGAAGTTTTTGAGAAGGTCGAACCTTCAGTTGGCTTCAACATTGAGATCAAATTCGACGACTACATTGTTTACCAGGAGGACTATCTCGTCCGTGTCCTTCAATCCATCTTGAAAGTTGTGTTTGAGTACGCCAAAGATAGGCCTATTGTATTCTCGTCATTCCAACCAGATGCAGCGGTCCTTGTTAAGAAGTTGCAGAACACCTACCCTGTCTTTTTCTTGACAAATGGTGGGAATGAGATTTACTGTGATGTGAGAAGGAATTCTTTGGATGAAGCAATAAAGGTGTGCTTAGAGGGTGGTTTGGAAGGCATTGTATCGGAGGTGAAAGGTGTTTTCCGAAATCCTGGGGCGGTAACCAAGATTAAGGAGTCCAAGCTTTCACTTCTAACCTATGGCAAACTAAATAATGTTGCCGAAGCGGTGTACATGCAACACCTAATGGGAATTGAGGGAGTGATTGTGGATTTTGTGCAAGAGATCACAGAAGCAGTGTGTGATATGATAAAGCCATCGGCGGCCACGGAAGAAGACGGGGATGATTTGTTGGAAGGGGACGGGAAGATGCAATTGAAATCAAAGCCGGAGTTCTCGCAGAGGGAGCTTTCCTTTCTGTTGAAGCTCATTCCAGAATTGATACAACTCTGA

Protein Analysis

431

Amino Acids

48.87

Weight (kDa)

6.31

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDPD PF03009 98 - 375 5e-46 Glycerophosphoryl diester phosphodiesterase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011688)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02040
fragaria_vesca FvH4_6g53990 FvH4_6g53990
malus_domestica MD09G1006500.v1.1 MD17G1010400.v1.1
prunus_persica Prupe.3G310500_v2.0.a1 Prupe.3G310500_v2.0.a1
pyrus_communis pycom111g00480 pycom17g00500
rosa_chinensis RchiOBHm_Chr2g0176161
rosa_laevigata RLG00000022386
rosa_multiflora Rmu_sc0000405.1_g000006
rosa_roxburghii Rroxscaffold_2G00076720
rosa_rugosa Rorug02G0591200
rosa_samantha Rh2AG669800 Rh2BG681400 Rh2CG644300 Rh2DG695100
rosa_wichuraiana Rw2G054950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 734
AatII GACGTC 1 cut(s) 174
AciI CCGC 5 cut(s) 226, 824, 1001, 1070, 1166
AcoI YGGCCR 1 cut(s) 1167
AcsI RAATTY 4 cut(s) 245, 407, 698, 910
AcuI CTGAAG 2 cut(s) 194, 657
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 5 cut(s) 218, 484, 640, 776, 1076
AfiI CCNNNNNNNGG 3 cut(s) 187, 677, 786
AflII CTTAAG 1 cut(s) 149
AgsI TTSAA 9 cut(s) 48, 115, 373, 620, 685, 749, 760, 1222, 1267
AjnI CCWGG 2 cut(s) 720, 994
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AluBI AGCT 4 cut(s) 214, 1027, 1252, 1270
AluI AGCT 4 cut(s) 214, 1027, 1252, 1270
Alw26I GTCTC 1 cut(s) 169
AoxI GGCC 2 cut(s) 788, 1167
ApeKI GCWGC 4 cut(s) 380, 435, 554, 821
ApoI RAATTY 4 cut(s) 245, 407, 698, 910
Asp700I GAANNNNTTC 1 cut(s) 910
AspS9I GGNCC 4 cut(s) 69, 190, 539, 826
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 460, 985
AvaII GGWCC 4 cut(s) 69, 190, 539, 826
BbsI GAAGAC 2 cut(s) 103, 1185
BbvI GCAGC 4 cut(s) 367, 422, 566, 833
BccI CCATC 6 cut(s) 368, 425, 557, 603, 761, 1168
BciT130I CCWGG 2 cut(s) 722, 996
BciVI GTATCC 1 cut(s) 130
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 1 cut(s) 281
BfrI CTTAAG 1 cut(s) 149
BfuI GTATCC 1 cut(s) 130
BisI GCNGC 5 cut(s) 381, 436, 555, 822, 1167
BlsI GCNGC 5 cut(s) 382, 437, 556, 823, 1168
Bme1390I CCNGG 3 cut(s) 194, 722, 996
Bme18I GGWCC 4 cut(s) 69, 190, 539, 826
BmgT120I GGNCC 4 cut(s) 69, 190, 539, 826
BmiI GGNNCC 2 cut(s) 71, 192
BmrFI CCNGG 3 cut(s) 194, 722, 996
BmsI GCATC 3 cut(s) 269, 808, 1203
BpiI GAAGAC 2 cut(s) 103, 1185
BpuEI CTTGAG 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 3 cut(s) 65, 995, 1170
BsaXI ACNNNNNCTCC 2 cut(s) 1095, 1125
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 677, 786
BseBI CCWGG 2 cut(s) 722, 996
BseDI CCNNGG 3 cut(s) 65, 995, 1170
BseGI GGATG 4 cut(s) 462, 568, 925, 1192
BseLI CCNNNNNNNGG 3 cut(s) 187, 677, 786
BseRI GAGGAG 1 cut(s) 18
BseXI GCAGC 4 cut(s) 367, 422, 566, 833
BshFI GGCC 2 cut(s) 790, 1169
BsiSI CCGG 2 cut(s) 194, 1232
BslFI GGGAC 4 cut(s) 55, 158, 176, 1217
BslI CCNNNNNNNGG 3 cut(s) 187, 677, 786
BsmAI GTCTC 1 cut(s) 169
BsmFI GGGAC 4 cut(s) 55, 158, 176, 1217
BsnI GGCC 2 cut(s) 790, 1169
Bsp1407I TGTACA 1 cut(s) 1074
Bsp143I GATC 6 cut(s) 184, 285, 328, 475, 693, 1128
BspACI CCGC 5 cut(s) 226, 824, 1001, 1070, 1166
BspANI GGCC 2 cut(s) 790, 1169
BspLI GGNNCC 2 cut(s) 71, 192
BspTI CTTAAG 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 1074
BssECI CCNNGG 3 cut(s) 65, 995, 1170
BssMI GATC 6 cut(s) 184, 285, 328, 475, 693, 1128
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 65
Bst2UI CCWGG 2 cut(s) 722, 996
Bst4CI ACNGT 5 cut(s) 321, 487, 513, 596, 896
Bst6I CTCTTC 2 cut(s) 213, 491
BstACI GRCGYC 1 cut(s) 171
BstAFI CTTAAG 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 1074
BstDEI CTNAG 2 cut(s) 273, 940
BstDSI CCRYGG 1 cut(s) 1170
BstEII GGTNACC 2 cut(s) 421, 1003
BstENI CCTNNNNNAGG 1 cut(s) 185
BstF5I GGATG 4 cut(s) 462, 568, 925, 1192
BstKTI GATC 6 cut(s) 187, 288, 331, 478, 696, 1131
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 6 cut(s) 184, 285, 328, 475, 693, 1128
BstMWI GCNNNNNNNGC 2 cut(s) 152, 1249
BstNI CCWGG 2 cut(s) 722, 996
BstNSI RCATGY 1 cut(s) 1081
BstPI GGTNACC 2 cut(s) 421, 1003
BstSCI CCNGG 3 cut(s) 192, 720, 994
BstV1I GCAGC 4 cut(s) 367, 422, 566, 833
BstV2I GAAGAC 2 cut(s) 103, 1185
BstXI CCANNNNNNTGG 1 cut(s) 241
BsuI GTATCC 1 cut(s) 130
BsuRI GGCC 2 cut(s) 790, 1169
BtgI CCRYGG 1 cut(s) 1170
BtsCI GGATG 4 cut(s) 462, 568, 925, 1192
BtsI GCAGTG 2 cut(s) 375, 1146
BtsIMutI CAGTG 2 cut(s) 375, 1146
Cfr13I GGNCC 4 cut(s) 69, 190, 539, 826
Csp6I GTAC 5 cut(s) 217, 483, 639, 775, 1075
CviAII CATG 3 cut(s) 161, 364, 1078
CviQI GTAC 5 cut(s) 217, 483, 639, 775, 1075
DdeI CTNAG 2 cut(s) 273, 940
DpnI GATC 6 cut(s) 186, 287, 330, 477, 695, 1130
DpnII GATC 6 cut(s) 184, 285, 328, 475, 693, 1128
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 1 cut(s) 1167
Eam1104I CTCTTC 2 cut(s) 213, 491
EarI CTCTTC 2 cut(s) 213, 491
Eco130I CCWWGG 1 cut(s) 65
Eco147I AGGCCT 1 cut(s) 790
Eco47I GGWCC 4 cut(s) 69, 190, 539, 826
Eco57I CTGAAG 2 cut(s) 194, 657
Eco91I GGTNACC 2 cut(s) 421, 1003
EcoNI CCTNNNNNAGG 1 cut(s) 185
EcoO109I RGGNCCY 2 cut(s) 69, 190
EcoO65I GGTNACC 2 cut(s) 421, 1003
EcoRI GAATTC 1 cut(s) 910
EcoRII CCWGG 2 cut(s) 720, 994
EcoT14I CCWWGG 1 cut(s) 65
ErhI CCWWGG 1 cut(s) 65
FaeI CATG 3 cut(s) 164, 367, 1081
FaiI YATR 8 cut(s) 17, 143, 162, 365, 537, 1044, 1079, 1151
FalI AAGNNNNNCTT 4 cut(s) 898, 930, 923, 955
FaqI GGGAC 4 cut(s) 55, 158, 176, 1217
FatI CATG 3 cut(s) 160, 363, 1077
FauI CCCGC 1 cut(s) 233
Fnu4HI GCNGC 5 cut(s) 381, 436, 555, 822, 1167
FokI GGATG 4 cut(s) 449, 575, 932, 1199
Fsp4HI GCNGC 5 cut(s) 381, 436, 555, 822, 1167
FspBI CTAG 1 cut(s) 281
GluI GCNGC 5 cut(s) 381, 436, 555, 822, 1167
HaeIII GGCC 2 cut(s) 790, 1169
HapII CCGG 2 cut(s) 194, 1232
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 3 cut(s) 164, 367, 1081
HindIII AAGCTT 1 cut(s) 1025
HinfI GANTC 2 cut(s) 629, 1019
HpaII CCGG 2 cut(s) 194, 1232
HphI GGTGA 2 cut(s) 460, 985
Hpy166II GTNNAC 3 cut(s) 542, 718, 1075
Hpy188I TCNGA 7 cut(s) 169, 290, 328, 333, 970, 989, 1295
Hpy188III TCNNGA 5 cut(s) 264, 395, 757, 868, 1277
Hpy8I GTNNAC 3 cut(s) 542, 718, 1075
Hpy99I CGWCG 3 cut(s) 173, 458, 707
HpyAV CCTTC 6 cut(s) 655, 681, 755, 900, 950, 1193
HpyCH4III ACNGT 5 cut(s) 321, 487, 513, 596, 896
HpyCH4IV ACGT 2 cut(s) 171, 414
HpyCH4V TGCA 5 cut(s) 821, 844, 1081, 1123, 1216
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 1249
HpyF3I CTNAG 2 cut(s) 273, 940
HpySE526I ACGT 2 cut(s) 171, 414
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 3 cut(s) 164, 367, 1081
Kzo9I GATC 6 cut(s) 184, 285, 328, 475, 693, 1128
LmnI GCTCC 1 cut(s) 1249
Lsp1109I GCAGC 4 cut(s) 367, 422, 566, 833
LweI GCATC 3 cut(s) 269, 808, 1203
MaeI CTAG 1 cut(s) 281
MaeII ACGT 2 cut(s) 171, 414
MaeIII GTNAC 2 cut(s) 421, 1003
MalI GATC 6 cut(s) 186, 287, 330, 477, 695, 1130
MboI GATC 6 cut(s) 184, 285, 328, 475, 693, 1128
MboII GAAGA 7 cut(s) 103, 200, 485, 508, 1187, 1190, 1222
MfeI CAATTG 1 cut(s) 1217
MluCI AATT 9 cut(s) 245, 407, 532, 600, 698, 910, 1095, 1217, 1280
MlyI GAGTC 1 cut(s) 1028
MmeI TCCRAC 3 cut(s) 192, 835, 1176
MroXI GAANNNNTTC 1 cut(s) 910
MseI TTAA 5 cut(s) 150, 348, 578, 834, 1014
MspA1I CMGCKG 2 cut(s) 214, 824
MspCI CTTAAG 1 cut(s) 149
MspI CCGG 2 cut(s) 194, 1232
MspR9I CCNGG 3 cut(s) 194, 722, 996
MunI CAATTG 1 cut(s) 1217
MvaI CCWGG 2 cut(s) 722, 996
MwoI GCNNNNNNNGC 2 cut(s) 152, 1249
NciI CCSGG 1 cut(s) 194
NdeII GATC 6 cut(s) 184, 285, 328, 475, 693, 1128
NlaIII CATG 3 cut(s) 164, 367, 1081
NlaIV GGNNCC 2 cut(s) 71, 192
NspI RCATGY 1 cut(s) 1081
PceI AGGCCT 1 cut(s) 790
PdmI GAANNNNTTC 1 cut(s) 910
PfeI GAWTC 1 cut(s) 629
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 5 cut(s) 382, 437, 556, 823, 1168
PleI GAGTC 1 cut(s) 1027
PpsI GAGTC 1 cut(s) 1027
PpuMI RGGWCCY 2 cut(s) 69, 190
Psp5II RGGWCCY 2 cut(s) 69, 190
Psp6I CCWGG 2 cut(s) 720, 994
PspEI GGTNACC 2 cut(s) 421, 1003
PspGI CCWGG 2 cut(s) 720, 994
PspN4I GGNNCC 2 cut(s) 71, 192
PspPI GGNCC 4 cut(s) 69, 190, 539, 826
PspPPI RGGWCCY 2 cut(s) 69, 190
PvuII CAGCTG 1 cut(s) 214
RsaI GTAC 5 cut(s) 218, 484, 640, 776, 1076
RsaNI GTAC 5 cut(s) 217, 483, 639, 775, 1075
SaqAI TTAA 5 cut(s) 150, 348, 578, 834, 1014
SatI GCNGC 5 cut(s) 381, 436, 555, 822, 1167
Sau3AI GATC 6 cut(s) 184, 285, 328, 475, 693, 1128
Sau96I GGNCC 4 cut(s) 69, 190, 539, 826
SchI GAGTC 1 cut(s) 1028
ScrFI CCNGG 3 cut(s) 194, 722, 996
SfaNI GCATC 3 cut(s) 269, 808, 1203
SinI GGWCC 4 cut(s) 69, 190, 539, 826
SmlI CTYRAG 2 cut(s) 7, 149
SmoI CTYRAG 2 cut(s) 7, 149
Sse9I AATT 9 cut(s) 245, 407, 532, 600, 698, 910, 1095, 1217, 1280
SseBI AGGCCT 1 cut(s) 790
SsiI CCGC 5 cut(s) 226, 824, 1001, 1070, 1166
SspMI CTAG 1 cut(s) 281
StuI AGGCCT 1 cut(s) 790
StyD4I CCNGG 3 cut(s) 192, 720, 994
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 5 cut(s) 321, 487, 513, 596, 896
TaiI ACGT 2 cut(s) 174, 417
TaqI TCGA 4 cut(s) 183, 263, 666, 702
TasI AATT 9 cut(s) 245, 407, 532, 600, 698, 910, 1095, 1217, 1280
TatI WGTACW 2 cut(s) 216, 1074
TauI GCSGC 1 cut(s) 1169
TfiI GAWTC 1 cut(s) 629
Tru1I TTAA 5 cut(s) 150, 348, 578, 834, 1014
Tru9I TTAA 5 cut(s) 150, 348, 578, 834, 1014
TscAI CASTG 2 cut(s) 382, 1146
TseI GCWGC 4 cut(s) 380, 435, 554, 821
TspDTI ATGAA 3 cut(s) 323, 451, 936
TspGWI ACGGA 3 cut(s) 536, 728, 1187
TspRI CASTG 2 cut(s) 382, 1146
Vha464I CTTAAG 1 cut(s) 149
VpaK11BI GGWCC 4 cut(s) 69, 190, 539, 826
XagI CCTNNNNNAGG 1 cut(s) 185
XapI RAATTY 4 cut(s) 245, 407, 698, 910
XceI RCATGY 1 cut(s) 1081
XmnI GAANNNNTTC 1 cut(s) 910
XspI CTAG 1 cut(s) 281
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.