RchiOBHm_Chr3g0449501

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0003432
Physical Location & Seq
Forward (+)
1260341 .. 1264203
3863 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41686

Sequence Viewer

Length: 1716 bp
ATGGAACCATTAAATCAGAAGTCTATAGAGAGAGTGGTTTCACAGAAGGCTTTGCAAATGGGGAGTTCATTTCCATGTCAAATCTGTGTGGTGGGTTTGCTCTGTGGAATCTGTCTTACTTCTTTGTTTATGGCTGCTCTCACTTCATTTGGTACTTTTGAGTTTGGTGGGTTTTCGTTTTCGACCATAGCTGAGGGTGCTTCAGCTGGGCACTCAAGCTCAGAGTTCATCAACTGCAGTTCTGAACCAATGATAACACAGAGAGTGGTAGTCGATTCGCTTGGAAGTGAAGAAACCATCAATAGTGAGAGAGCCTCATTATTGTATTCAGCTTGGAGTGCTTTACTAACTGAATCAGATTCCGGAAAAAGTGATTACTTGCAGAGAAATGGACTAAACAGATCCAGTGTGCCAAACACCCCTCATTTGGAAAACTGCAAGTTGAAAGGACAAGTACACAAGCGCCTTGATAAACATCCCGAGAATGAGAGCCATCCAACAGCTGCAACAAATGAGCAGTCTAGCTATTTTAGGAATCAAGCTATGTCTGAAACTGCTTATGCTCCTTGGATTACAGGGTCTGACGAAGAAAATTACCCGTCAACACGAAAAGTGCAACGAGACATATGGCTGCATCAGCATCCTTTGAACTGCTGGGATCCTAGTGTAAAGTTTCTCGTAGCTGACTGGGAAAGATTACCTGGATTGGGTATTGGAGCCCAGATTGCTGGAATGTGTGGAGTTCTTGCTATTGCAATGAAGGAAAAAAGGGTCCTTGTTACCAACTACTTTAATCGAGCTGACCATGATGGTTGCAAAGGTTCGTCCCGCTCCAGATGGTCCTGTTATTTTTTCCCAGAAACATCTCTAGAATGTCGAAATCGTGCATTGGAGCTTATAGAAAGTGAAGATGCCTGGAAAAGTGGCACCATCAAGGCAAAAGATAATTATACTTCAAAGGAAATATGGGCTGGACGAACACCTAGAATATGGGGTGATCCTTGGAGTCATTTGCAACCAACAACAGAAATAAATGGGACTTTGATTGCTTATCATCGCAAAATGGATCGAAGGTGGTGGCGAGCACAGGCAGTACGATACTTGATGCGATTTCAAACCGAGTACACATGTGACTTGCTGAATGTTGCTCGCCATGCGGCATTTGGAAAGCAAGCTGCCGATATGGTAGTCAGAAATTTCGAGGGGGAATGGTCCAAGGTGGATGTTACAGTGAATGAAAGGTCAGATATTGAAGAGTTTGTTTGGTCTAATCACAAGCCATGGATGCCTAGGCCACTACTGAGCATGCATGTAAGGATGGGAGACAAAGCATGTGAAATGAAGGTAGTTGAATTTCAAGAATACATGAGTCTTGCTGGCCGCATTAGAAAGCGATTTCCACAGCTCAACAGTGTTTGGCTTTCCACTGAAATGCAGGAAGTGATTGATAAATCTAAAGGATACCCTCATTGGGACTTCTACTACACAAATGTGACACGTCAAGTGGGAAACATGACAATGGCTGCTTATGAAACAAGTCTTGGTAGAAAAACCAGCACGAACTACCCGCTTGTCAATTTCTTGATGGCAACCGAAGCTGACTTTTTCATTGGAGCGTTGGGTTCTACTTGGTGCTTCCTTATAGATGGAATGAGAAACACCGGAGGGAAAGTTATGGCTGGATATTTGAGCGTCAACAAGGACCGTTTCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

571

Amino Acids

64.94

Weight (kDa)

7.54

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 926
AccBSI CCGCTC 1 cut(s) 831
AccIII TCCGGA 1 cut(s) 362
AciI CCGC 4 cut(s) 829, 1157, 1381, 1568
AclWI GGATC 5 cut(s) 396, 653, 666, 992, 1074
AcoI YGGCCR 1 cut(s) 1378
AcsI RAATTY 2 cut(s) 1195, 1352
AcuI CTGAAG 1 cut(s) 186
AfaI GTAC 4 cut(s) 154, 456, 1095, 1124
AfiI CCNNNNNNNGG 3 cut(s) 427, 707, 1471
AflIII ACRYGT 2 cut(s) 1127, 1496
AgsI TTSAA 7 cut(s) 445, 649, 957, 1115, 1253, 1352, 1358
AjiI CACGTC 1 cut(s) 1499
AjnI CCWGG 2 cut(s) 700, 914
AjuI GAANNNNNNNTTGG 2 cut(s) 1524, 1556
AleI CACNNNNGTG 1 cut(s) 1490
Alw21I GWGCWC 1 cut(s) 1087
Alw26I GTCTC 2 cut(s) 615, 1317
AlwI GGATC 5 cut(s) 396, 653, 666, 992, 1074
AlwNI CAGNNNCTG 1 cut(s) 581
Ama87I CYCGRG 1 cut(s) 479
Aor13HI TCCGGA 1 cut(s) 362
AoxI GGCC 2 cut(s) 1292, 1378
ApeKI GCWGC 5 cut(s) 134, 503, 631, 1175, 1523
ApoI RAATTY 2 cut(s) 1195, 1352
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AspA2I CCTAGG 1 cut(s) 1289
AspLEI GCGC 1 cut(s) 465
AspS9I GGNCC 4 cut(s) 772, 840, 1212, 1702
AsuHPI GGTGA 1 cut(s) 1007
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 4 cut(s) 772, 840, 1212, 1702
AvrII CCTAGG 1 cut(s) 1289
BaeGI GKGCMC 1 cut(s) 213
BamHI GGATCC 1 cut(s) 658
BanI GGYRCC 1 cut(s) 926
BanII GRGCYC 1 cut(s) 721
Bbv12I GWGCWC 1 cut(s) 1087
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 5 cut(s) 121, 490, 618, 1162, 1510
BccI CCATC 8 cut(s) 305, 501, 803, 831, 938, 1312, 1579, 1640
BciT130I CCWGG 2 cut(s) 702, 916
BciVI GTATCC 1 cut(s) 1454
BcoDI GTCTC 2 cut(s) 615, 1317
BfaI CTAG 5 cut(s) 522, 663, 869, 984, 1290
BfmI CTRYAG 2 cut(s) 24, 235
BfoI RGCGCY 1 cut(s) 466
BfuI GTATCC 1 cut(s) 1454
BisI GCNGC 7 cut(s) 135, 504, 632, 1158, 1176, 1381, 1524
BlnI CCTAGG 1 cut(s) 1289
BlsI GCNGC 7 cut(s) 136, 505, 633, 1159, 1177, 1382, 1525
Bme1390I CCNGG 2 cut(s) 702, 916
Bme18I GGWCC 4 cut(s) 772, 840, 1212, 1702
BmeT110I CYCGRG 1 cut(s) 479
BmgBI CACGTC 1 cut(s) 1499
BmgT120I GGNCC 4 cut(s) 772, 840, 1212, 1702
BmiI GGNNCC 5 cut(s) 6, 660, 718, 773, 928
BmrFI CCNGG 2 cut(s) 702, 916
BmrI ACTGGG 1 cut(s) 697
BmsI GCATC 5 cut(s) 643, 649, 901, 1095, 1275
BmuI ACTGGG 1 cut(s) 697
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BpmI CTGGAG 1 cut(s) 817
Bpu10I CCTNAGC 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 199
BsaBI GATNNNNATC 1 cut(s) 474
BsaJI CCNNGG 5 cut(s) 566, 1001, 1215, 1280, 1289
BsaWI WCCGGW 2 cut(s) 362, 1661
BsaXI ACNNNNNCTCC 2 cut(s) 1656, 1686
Bsc4I CCNNNNNNNGG 3 cut(s) 427, 707, 1471
Bse1I ACTGG 2 cut(s) 405, 692
Bse3DI GCAATG 1 cut(s) 762
Bse8I GATNNNNATC 1 cut(s) 474
BseAI TCCGGA 1 cut(s) 362
BseBI CCWGG 2 cut(s) 702, 916
BseDI CCNNGG 5 cut(s) 566, 1001, 1215, 1280, 1289
BseGI GGATG 6 cut(s) 475, 493, 640, 1228, 1290, 1323
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 3 cut(s) 427, 707, 1471
BseMI GCAATG 1 cut(s) 762
BseMII CTCAG 3 cut(s) 183, 234, 1292
BseNI ACTGG 2 cut(s) 405, 692
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 5 cut(s) 121, 490, 618, 1162, 1510
BseYI CCCAGC 2 cut(s) 206, 654
BshFI GGCC 2 cut(s) 1294, 1380
BshNI GGYRCC 1 cut(s) 926
BsiHKAI GWGCWC 1 cut(s) 1087
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 2 cut(s) 363, 1662
BslFI GGGAC 3 cut(s) 811, 1051, 1487
BslI CCNNNNNNNGG 3 cut(s) 427, 707, 1471
BsmAI GTCTC 2 cut(s) 615, 1317
BsmFI GGGAC 3 cut(s) 811, 1051, 1487
BsnI GGCC 2 cut(s) 1294, 1380
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 3 cut(s) 213, 721, 1087
Bsp13I TCCGGA 1 cut(s) 362
Bsp143I GATC 4 cut(s) 401, 658, 997, 1066
Bsp19I CCATGG 1 cut(s) 1280
BspACI CCGC 4 cut(s) 829, 1157, 1381, 1568
BspANI GGCC 2 cut(s) 1294, 1380
BspCNI CTCAG 3 cut(s) 184, 233, 1293
BspEI TCCGGA 1 cut(s) 362
BspLI GGNNCC 5 cut(s) 6, 660, 718, 773, 928
BspMAI CTGCAG 1 cut(s) 239
BspPI GGATC 5 cut(s) 396, 653, 666, 992, 1074
BspT107I GGYRCC 1 cut(s) 926
BsrBI CCGCTC 1 cut(s) 831
BsrDI GCAATG 1 cut(s) 762
BsrI ACTGG 2 cut(s) 405, 692
BssECI CCNNGG 5 cut(s) 566, 1001, 1215, 1280, 1289
BssMI GATC 4 cut(s) 401, 658, 997, 1066
BssT1I CCWWGG 5 cut(s) 566, 1001, 1215, 1280, 1289
Bst2UI CCWGG 2 cut(s) 702, 916
Bst4CI ACNGT 3 cut(s) 1231, 1412, 1706
Bst6I CTCTTC 1 cut(s) 1248
BstC8I GCNNGC 5 cut(s) 1083, 1150, 1173, 1307, 1378
BstDEI CTNAG 3 cut(s) 192, 220, 1301
BstDSI CCRYGG 1 cut(s) 1280
BstF5I GGATG 6 cut(s) 475, 493, 640, 1228, 1290, 1323
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 1 cut(s) 465
BstKTI GATC 4 cut(s) 404, 661, 1000, 1069
BstMAI GTCTC 2 cut(s) 615, 1317
BstMBI GATC 4 cut(s) 401, 658, 997, 1066
BstMWI GCNNNNNNNGC 7 cut(s) 197, 338, 637, 725, 1154, 1285, 1595
BstNI CCWGG 2 cut(s) 702, 916
BstNSI RCATGY 4 cut(s) 1131, 1309, 1313, 1335
BstSCI CCNGG 2 cut(s) 700, 914
BstSFI CTRYAG 2 cut(s) 24, 235
BstSLI GKGCMC 1 cut(s) 213
BstV1I GCAGC 5 cut(s) 121, 490, 618, 1162, 1510
BstX2I RGATCY 2 cut(s) 401, 658
BstXI CCANNNNNNTGG 1 cut(s) 728
BstYI RGATCY 2 cut(s) 401, 658
BsuI GTATCC 1 cut(s) 1454
BsuRI GGCC 2 cut(s) 1294, 1380
BtgI CCRYGG 1 cut(s) 1280
BtgZI GCGATG 1 cut(s) 1040
BtrI CACGTC 1 cut(s) 1499
BtsCI GGATG 6 cut(s) 475, 493, 640, 1228, 1290, 1323
BtsIMutI CAGTG 4 cut(s) 412, 1236, 1417, 1425
Cac8I GCNNGC 5 cut(s) 1083, 1150, 1173, 1307, 1378
CaiI CAGNNNCTG 1 cut(s) 581
CfoI GCGC 1 cut(s) 465
Cfr13I GGNCC 4 cut(s) 772, 840, 1212, 1702
CseI GACGC 1 cut(s) 1681
Csp6I GTAC 4 cut(s) 153, 455, 1094, 1123
CviQI GTAC 4 cut(s) 153, 455, 1094, 1123
DdeI CTNAG 3 cut(s) 192, 220, 1301
DpnI GATC 4 cut(s) 403, 660, 999, 1068
DpnII GATC 4 cut(s) 401, 658, 997, 1066
EaeI YGGCCR 1 cut(s) 1378
Eam1104I CTCTTC 1 cut(s) 1248
EarI CTCTTC 1 cut(s) 1248
Eco130I CCWWGG 5 cut(s) 566, 1001, 1215, 1280, 1289
Eco24I GRGCYC 1 cut(s) 721
Eco47I GGWCC 4 cut(s) 772, 840, 1212, 1702
Eco57I CTGAAG 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 772
EcoRII CCWGG 2 cut(s) 700, 914
EcoT14I CCWWGG 5 cut(s) 566, 1001, 1215, 1280, 1289
EcoT22I ATGCAT 1 cut(s) 1311
EcoT38I GRGCYC 1 cut(s) 721
ErhI CCWWGG 5 cut(s) 566, 1001, 1215, 1280, 1289
FaqI GGGAC 3 cut(s) 811, 1051, 1487
FauI CCCGC 2 cut(s) 836, 1575
FauNDI CATATG 1 cut(s) 626
Fnu4HI GCNGC 7 cut(s) 135, 504, 632, 1158, 1176, 1381, 1524
FokI GGATG 6 cut(s) 462, 480, 627, 1235, 1297, 1330
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 7 cut(s) 135, 504, 632, 1158, 1176, 1381, 1524
FspBI CTAG 5 cut(s) 522, 663, 869, 984, 1290
GlaI GCGC 1 cut(s) 464
GluI GCNGC 7 cut(s) 135, 504, 632, 1158, 1176, 1381, 1524
GsaI CCCAGC 2 cut(s) 210, 658
GsuI CTGGAG 1 cut(s) 817
HaeII RGCGCY 1 cut(s) 466
HaeIII GGCC 2 cut(s) 1294, 1380
HapII CCGG 2 cut(s) 363, 1662
HgaI GACGC 1 cut(s) 1681
HhaI GCGC 1 cut(s) 465
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HincII GTYRAC 2 cut(s) 603, 1696
HindII GTYRAC 2 cut(s) 603, 1696
HinfI GANTC 7 cut(s) 108, 275, 353, 359, 535, 1006, 1369
HpaII CCGG 2 cut(s) 363, 1662
HphI GGTGA 1 cut(s) 1007
Hpy166II GTNNAC 4 cut(s) 457, 603, 1125, 1696
Hpy188I TCNGA 8 cut(s) 18, 223, 244, 358, 550, 583, 1193, 1246
Hpy188III TCNNGA 6 cut(s) 363, 479, 834, 869, 1358, 1582
Hpy8I GTNNAC 4 cut(s) 457, 603, 1125, 1696
HpyAV CCTTC 4 cut(s) 40, 754, 1065, 1336
HpyCH4III ACNGT 3 cut(s) 1231, 1412, 1706
HpyCH4IV ACGT 1 cut(s) 1498
HpyF10VI GCNNNNNNNGC 7 cut(s) 197, 338, 637, 725, 1154, 1285, 1595
HpyF3I CTNAG 3 cut(s) 192, 220, 1301
HpySE526I ACGT 1 cut(s) 1498
HspAI GCGC 1 cut(s) 463
Kpn2I TCCGGA 1 cut(s) 362
Kzo9I GATC 4 cut(s) 401, 658, 997, 1066
LmnI GCTCC 5 cut(s) 568, 716, 836, 892, 1613
Lsp1109I GCAGC 5 cut(s) 121, 490, 618, 1162, 1510
LweI GCATC 5 cut(s) 643, 649, 901, 1095, 1275
MaeI CTAG 5 cut(s) 522, 663, 869, 984, 1290
MaeII ACGT 1 cut(s) 1498
MaeIII GTNAC 4 cut(s) 778, 1130, 1225, 1492
MalI GATC 4 cut(s) 403, 660, 999, 1068
MbiI CCGCTC 1 cut(s) 831
MboI GATC 4 cut(s) 401, 658, 997, 1066
MboII GAAGA 4 cut(s) 302, 599, 920, 1265
MflI RGATCY 2 cut(s) 401, 658
MhlI GDGCHC 3 cut(s) 213, 721, 1087
MluCI AATT 5 cut(s) 592, 946, 1195, 1352, 1576
MlyI GAGTC 2 cut(s) 1015, 1378
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 6 cut(s) 187, 325, 432, 1195, 1476, 1658
Mph1103I ATGCAT 1 cut(s) 1311
MroI TCCGGA 1 cut(s) 362
MseI TTAA 2 cut(s) 11, 792
MslI CAYNNNNRTG 4 cut(s) 73, 1430, 1490, 1517
MspA1I CMGCKG 2 cut(s) 206, 503
MspI CCGG 2 cut(s) 363, 1662
MspR9I CCNGG 2 cut(s) 702, 916
MvaI CCWGG 2 cut(s) 702, 916
MwoI GCNNNNNNNGC 7 cut(s) 197, 338, 637, 725, 1154, 1285, 1595
NcoI CCATGG 1 cut(s) 1280
NdeI CATATG 1 cut(s) 626
NdeII GATC 4 cut(s) 401, 658, 997, 1066
NlaIV GGNNCC 5 cut(s) 6, 660, 718, 773, 928
NmuCI GTSAC 2 cut(s) 1130, 1492
NsiI ATGCAT 1 cut(s) 1311
NspI RCATGY 4 cut(s) 1131, 1309, 1313, 1335
OliI CACNNNNGTG 1 cut(s) 1490
PaeI GCATGC 1 cut(s) 1309
PciI ACATGT 1 cut(s) 1127
PfeI GAWTC 5 cut(s) 108, 275, 353, 359, 535
PkrI GCNGC 7 cut(s) 136, 505, 633, 1159, 1177, 1382, 1525
PleI GAGTC 2 cut(s) 1014, 1377
PpsI GAGTC 2 cut(s) 1014, 1377
PpuMI RGGWCCY 1 cut(s) 772
PscI ACATGT 1 cut(s) 1127
Psp5II RGGWCCY 1 cut(s) 772
Psp6I CCWGG 2 cut(s) 700, 914
PspFI CCCAGC 2 cut(s) 206, 654
PspGI CCWGG 2 cut(s) 700, 914
PspN4I GGNNCC 5 cut(s) 6, 660, 718, 773, 928
PspPI GGNCC 4 cut(s) 772, 840, 1212, 1702
PspPPI RGGWCCY 1 cut(s) 772
PstI CTGCAG 1 cut(s) 239
PstNI CAGNNNCTG 1 cut(s) 581
PsuI RGATCY 2 cut(s) 401, 658
PvuII CAGCTG 2 cut(s) 206, 503
RsaI GTAC 4 cut(s) 154, 456, 1095, 1124
RsaNI GTAC 4 cut(s) 153, 455, 1094, 1123
RseI CAYNNNNRTG 4 cut(s) 73, 1430, 1490, 1517
SaqAI TTAA 2 cut(s) 11, 792
SatI GCNGC 7 cut(s) 135, 504, 632, 1158, 1176, 1381, 1524
Sau3AI GATC 4 cut(s) 401, 658, 997, 1066
Sau96I GGNCC 4 cut(s) 772, 840, 1212, 1702
SchI GAGTC 2 cut(s) 1015, 1378
ScrFI CCNGG 2 cut(s) 702, 916
SduI GDGCHC 3 cut(s) 213, 721, 1087
SfaNI GCATC 5 cut(s) 643, 649, 901, 1095, 1275
SfcI CTRYAG 2 cut(s) 24, 235
SinI GGWCC 4 cut(s) 772, 840, 1212, 1702
SmiMI CAYNNNNRTG 4 cut(s) 73, 1430, 1490, 1517
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
SphI GCATGC 1 cut(s) 1309
Sse9I AATT 5 cut(s) 592, 946, 1195, 1352, 1576
SsiI CCGC 4 cut(s) 829, 1157, 1381, 1568
SspMI CTAG 5 cut(s) 522, 663, 869, 984, 1290
StyD4I CCNGG 2 cut(s) 700, 914
StyI CCWWGG 5 cut(s) 566, 1001, 1215, 1280, 1289
TaaI ACNGT 3 cut(s) 1231, 1412, 1706
TaiI ACGT 1 cut(s) 1501
TaqI TCGA 6 cut(s) 182, 273, 796, 877, 1069, 1200
TasI AATT 5 cut(s) 592, 946, 1195, 1352, 1576
TatI WGTACW 2 cut(s) 454, 1122
TauI GCSGC 2 cut(s) 1160, 1383
TfiI GAWTC 5 cut(s) 108, 275, 353, 359, 535
Tru1I TTAA 2 cut(s) 11, 792
Tru9I TTAA 2 cut(s) 11, 792
TscAI CASTG 4 cut(s) 412, 1236, 1417, 1432
TseFI GTSAC 2 cut(s) 1130, 1492
TseI GCWGC 5 cut(s) 134, 503, 631, 1175, 1523
Tsp45I GTSAC 2 cut(s) 1130, 1492
TspDTI ATGAA 8 cut(s) 57, 135, 217, 773, 1251, 1355, 1545, 1597
TspRI CASTG 4 cut(s) 412, 1236, 1417, 1432
VpaK11BI GGWCC 4 cut(s) 772, 840, 1212, 1702
XapI RAATTY 2 cut(s) 1195, 1352
XbaI TCTAGA 1 cut(s) 868
XceI RCATGY 4 cut(s) 1131, 1309, 1313, 1335
XcmI CCANNNNNNNNNTGG 1 cut(s) 1160
XmaJI CCTAGG 1 cut(s) 1289
XspI CTAG 5 cut(s) 522, 663, 869, 984, 1290
Zsp2I ATGCAT 1 cut(s) 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.