RchiOBHm_Chr3g0476251

CDK5RAP3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0016943
Physical Location & Seq
Reverse (-)
22216022 .. 22217438
1417 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44168

Sequence Viewer

Length: 537 bp
ATGAGGTATATGACAGTTTGTTGTATGATCACAGGATTCAAAGCTTTTCTAAATCAGCGGTTGGTGGTGTTGAAGAATGAAGAAACTTTGTCATTGCAACATCAAGTCATCCAGGCAGTTGATCCCTTTGTGCTGCAGCAATATGTTTATGATGCCATTGAGACAGTGCTATCTGATGTTTGCTTGGCCATTTCTATGCTGAAAAAACGAGGAAACGCGGGACTTGATCATGATTCTCAACTCCAAAAGCATCACGAAGTGAAGTTGAAAGAGGGTTTGAAGGGCTTAGCTACCAAACGCATGGAGCTGCACAACTCGTTATCTTCTTCCTGGCCAAAGCAGGAAGCGGCTCTTGCAAAAACCTGCGAGCTTAAGAAGCTGTGTGAGAGCACCCTTTCCTCCATGTTTGATGGAAGGCCTGTTCATGAAGAGATTAATGCCCGTTTGAATAGTGGTGTTGCTGCATATGAAAATGTTTGTCTCTGGCGTGAAGCGTACAATATGAGCGTGTACTTTGAATCTCTTCGTTTTAACTAA

Protein Analysis

178

Amino Acids

20.3

Weight (kDa)

7.63

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDK5RAP3 PF05600 13 - 142 1.5e-20 CDK5 regulatory subunit-associated protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 371
AccII CGCG 1 cut(s) 218
AciI CCGC 3 cut(s) 58, 218, 347
AclWI GGATC 1 cut(s) 116
AcoI YGGCCR 2 cut(s) 186, 332
AdeI CACNNNGTG 1 cut(s) 259
AfaI GTAC 2 cut(s) 497, 512
AflII CTTAAG 1 cut(s) 371
AgsI TTSAA 6 cut(s) 40, 73, 268, 280, 448, 518
AjnI CCWGG 2 cut(s) 111, 329
AloI GAACNNNNNNTCC 2 cut(s) 405, 437
AluBI AGCT 5 cut(s) 44, 290, 307, 370, 379
AluI AGCT 5 cut(s) 44, 290, 307, 370, 379
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 2 cut(s) 155, 485
AlwI GGATC 1 cut(s) 116
AoxI GGCC 3 cut(s) 186, 332, 416
ApeKI GCWGC 4 cut(s) 133, 136, 307, 461
AseI ATTAAT 1 cut(s) 435
Asp700I GAANNNNTTC 1 cut(s) 522
BalI TGGCCA 2 cut(s) 188, 334
Bbv12I GWGCWC 1 cut(s) 392
BbvI GCAGC 4 cut(s) 120, 148, 294, 448
BccI CCATC 1 cut(s) 404
BciT130I CCWGG 2 cut(s) 113, 331
BclI TGATCA 2 cut(s) 27, 226
BcoDI GTCTC 2 cut(s) 155, 485
BfmI CTRYAG 1 cut(s) 134
BfrI CTTAAG 1 cut(s) 371
BfuAI ACCTGC 1 cut(s) 371
BisI GCNGC 5 cut(s) 134, 137, 308, 348, 462
BlpI GCTNAGC 1 cut(s) 286
BlsI GCNGC 5 cut(s) 135, 138, 309, 349, 463
Bme1390I CCNGG 2 cut(s) 113, 331
BmrFI CCNGG 2 cut(s) 113, 331
BmsI GCATC 2 cut(s) 142, 259
Bpu1102I GCTNAGC 1 cut(s) 286
Bse3DI GCAATG 1 cut(s) 92
BseBI CCWGG 2 cut(s) 113, 331
BseGI GGATG 1 cut(s) 108
BseMI GCAATG 1 cut(s) 92
BseXI GCAGC 4 cut(s) 120, 148, 294, 448
BsgI GTGCAG 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 3 cut(s) 188, 334, 418
BsiHKAI GWGCWC 1 cut(s) 392
BslFI GGGAC 1 cut(s) 234
BsmAI GTCTC 2 cut(s) 155, 485
BsmFI GGGAC 1 cut(s) 234
BsnI GGCC 3 cut(s) 188, 334, 418
Bsp1286I GDGCHC 1 cut(s) 392
Bsp143I GATC 3 cut(s) 27, 121, 226
Bsp1720I GCTNAGC 1 cut(s) 286
BspACI CCGC 3 cut(s) 58, 218, 347
BspANI GGCC 3 cut(s) 188, 334, 418
BspFNI CGCG 1 cut(s) 218
BspHI TCATGA 2 cut(s) 229, 424
BspMAI CTGCAG 1 cut(s) 138
BspMI ACCTGC 1 cut(s) 371
BspPI GGATC 1 cut(s) 116
BspTI CTTAAG 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 92
BssMI GATC 3 cut(s) 27, 121, 226
Bst2UI CCWGG 2 cut(s) 113, 331
Bst4CI ACNGT 2 cut(s) 16, 166
Bst6I CTCTTC 2 cut(s) 423, 528
BstAFI CTTAAG 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 1 cut(s) 286
BstF5I GGATG 1 cut(s) 108
BstFNI CGCG 1 cut(s) 218
BstKTI GATC 3 cut(s) 30, 124, 229
BstMAI GTCTC 2 cut(s) 155, 485
BstMBI GATC 3 cut(s) 27, 121, 226
BstMWI GCNNNNNNNGC 2 cut(s) 353, 376
BstNI CCWGG 2 cut(s) 113, 331
BstSCI CCNGG 2 cut(s) 111, 329
BstSFI CTRYAG 1 cut(s) 134
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 4 cut(s) 120, 148, 294, 448
BstXI CCANNNNNNTGG 1 cut(s) 301
BsuRI GGCC 3 cut(s) 188, 334, 418
BtsCI GGATG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 171
BveI ACCTGC 1 cut(s) 371
Cac8I GCNNGC 1 cut(s) 368
CciI TCATGA 2 cut(s) 229, 424
Csp6I GTAC 2 cut(s) 496, 511
CviAII CATG 4 cut(s) 230, 301, 403, 425
CviQI GTAC 2 cut(s) 496, 511
DdeI CTNAG 1 cut(s) 286
DpnI GATC 3 cut(s) 29, 123, 228
DpnII GATC 3 cut(s) 27, 121, 226
DraIII CACNNNGTG 1 cut(s) 259
EaeI YGGCCR 2 cut(s) 186, 332
Eam1104I CTCTTC 2 cut(s) 423, 528
EarI CTCTTC 2 cut(s) 423, 528
Eco147I AGGCCT 1 cut(s) 418
EcoRII CCWGG 2 cut(s) 111, 329
FaeI CATG 4 cut(s) 233, 304, 406, 428
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 1 cut(s) 234
FatI CATG 4 cut(s) 229, 300, 402, 424
FauI CCCGC 1 cut(s) 211
FauNDI CATATG 1 cut(s) 466
FbaI TGATCA 2 cut(s) 27, 226
Fnu4HI GCNGC 5 cut(s) 134, 137, 308, 348, 462
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 5 cut(s) 134, 137, 308, 348, 462
GluI GCNGC 5 cut(s) 134, 137, 308, 348, 462
HaeIII GGCC 3 cut(s) 188, 334, 418
Hin1II CATG 4 cut(s) 233, 304, 406, 428
HindIII AAGCTT 1 cut(s) 42
HinfI GANTC 3 cut(s) 36, 233, 518
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 1 cut(s) 175
Hpy188III TCNNGA 3 cut(s) 230, 254, 425
Hpy8I GTNNAC 1 cut(s) 511
HpyAV CCTTC 2 cut(s) 274, 408
HpyCH4III ACNGT 2 cut(s) 16, 166
HpyCH4V TGCA 5 cut(s) 97, 136, 310, 356, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 376
HpyF3I CTNAG 1 cut(s) 286
Hsp92II CATG 4 cut(s) 233, 304, 406, 428
Ksp22I TGATCA 2 cut(s) 27, 226
Kzo9I GATC 3 cut(s) 27, 121, 226
LmnI GCTCC 1 cut(s) 304
LpnPI CCDG 9 cut(s) 18, 98, 125, 316, 326, 343, 376, 432, 469
Lsp1109I GCAGC 4 cut(s) 120, 148, 294, 448
LweI GCATC 2 cut(s) 142, 259
MalI GATC 3 cut(s) 29, 123, 228
MboI GATC 3 cut(s) 27, 121, 226
MboII GAAGA 6 cut(s) 85, 92, 315, 318, 440, 515
MhlI GDGCHC 1 cut(s) 392
MlsI TGGCCA 2 cut(s) 188, 334
MluNI TGGCCA 2 cut(s) 188, 334
MnlI CCTC 3 cut(s) 203, 265, 409
Mox20I TGGCCA 2 cut(s) 188, 334
MroXI GAANNNNTTC 1 cut(s) 522
MscI TGGCCA 2 cut(s) 188, 334
MseI TTAA 3 cut(s) 372, 435, 531
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 2 cut(s) 188, 334
MspA1I CMGCKG 1 cut(s) 58
MspCI CTTAAG 1 cut(s) 371
MspR9I CCNGG 2 cut(s) 113, 331
MvaI CCWGG 2 cut(s) 113, 331
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 2 cut(s) 353, 376
NdeI CATATG 1 cut(s) 466
NdeII GATC 3 cut(s) 27, 121, 226
NlaIII CATG 4 cut(s) 233, 304, 406, 428
PagI TCATGA 2 cut(s) 229, 424
PceI AGGCCT 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 3 cut(s) 36, 233, 518
PkrI GCNGC 5 cut(s) 135, 138, 309, 349, 463
PshBI ATTAAT 1 cut(s) 435
Psp6I CCWGG 2 cut(s) 111, 329
PspGI CCWGG 2 cut(s) 111, 329
PstI CTGCAG 1 cut(s) 138
RsaI GTAC 2 cut(s) 497, 512
RsaNI GTAC 2 cut(s) 496, 511
RseI CAYNNNNRTG 1 cut(s) 194
SaqAI TTAA 3 cut(s) 372, 435, 531
SatI GCNGC 5 cut(s) 134, 137, 308, 348, 462
Sau3AI GATC 3 cut(s) 27, 121, 226
ScrFI CCNGG 2 cut(s) 113, 331
SduI GDGCHC 1 cut(s) 392
SetI ASST 7 cut(s) 8, 46, 292, 309, 365, 372, 381
SfaNI GCATC 2 cut(s) 142, 259
SfcI CTRYAG 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SseBI AGGCCT 1 cut(s) 418
SsiI CCGC 3 cut(s) 58, 218, 347
StuI AGGCCT 1 cut(s) 418
StyD4I CCNGG 2 cut(s) 111, 329
TaaI ACNGT 2 cut(s) 16, 166
TatI WGTACW 1 cut(s) 510
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 3 cut(s) 36, 233, 518
Tru1I TTAA 3 cut(s) 372, 435, 531
Tru9I TTAA 3 cut(s) 372, 435, 531
TscAI CASTG 1 cut(s) 171
TseI GCWGC 4 cut(s) 133, 136, 307, 461
TspDTI ATGAA 4 cut(s) 93, 413, 441, 483
TspRI CASTG 1 cut(s) 171
Vha464I CTTAAG 1 cut(s) 371
VspI ATTAAT 1 cut(s) 435
XmnI GAANNNNTTC 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.