Rh3DG229900

CDK5RAP3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0016943
Physical Location & Seq
Reverse (-)
21871818 .. 21872999
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG229900.1

Sequence Viewer

Length: 534 bp
ATGAAGAAACTTTGTCATTTGCAACATCAAGTCATCCAGGCAGTTGATCCCTTTGTGCTGCAGCAATATGTTTATGATGCCATTGAGACAGTGCTATCTGATGTTTGCTTGGCCATTTCTATGCTGAAAAACGAGGAAACGCGGGACTCGATCATGATTCTCGACTCCAAAAGATTTCTAGACAGACTAATGAGTTCATTGGAAGAAAAGAAGCATCACGAAGTGAAGTTGAAAGAGGGTTTGAAGGGCTTAGCTACCAAACGCATGGAGCTGCACAACTCGTTATCTTCTTCCTGGCCAAAGCAGGAAGCGGCTCTTGCAAAAACCCGCGAGCTTAAGAAGCTGTGTGAGAGCACCCTTTCCTCCATGCTTGATGGAAGACCTGTTCATATAATTGAAGAGATTAATGCCCGTTTGAATAGTGGTGTTGCTGCATATGAAAATGTTTGTCTCTGGGGTGAAGCGTACAATATGAGCGTGTACTTTGAACCTCTTCGTTTTTACTTACTAACTTTGGGGTGTCTTCGGGGGTAA

Protein Analysis

177

Amino Acids

20.27

Weight (kDa)

7.0

Isoelectric Point (pI)

57.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDK5RAP3 PF05600 7 - 132 5.9e-34 CDK5 regulatory subunit-associated protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 142, 330
AciI CCGC 3 cut(s) 142, 311, 328
AclWI GGATC 1 cut(s) 41
AcoI YGGCCR 2 cut(s) 111, 296
AdeI CACNNNGTG 1 cut(s) 223
AfaI GTAC 2 cut(s) 467, 482
AflII CTTAAG 1 cut(s) 335
AgsI TTSAA 5 cut(s) 232, 244, 398, 418, 488
AjnI CCWGG 2 cut(s) 36, 293
AloI GAACNNNNNNTCC 2 cut(s) 369, 401
AluBI AGCT 4 cut(s) 254, 271, 334, 343
AluI AGCT 4 cut(s) 254, 271, 334, 343
Alw21I GWGCWC 1 cut(s) 356
Alw26I GTCTC 2 cut(s) 80, 455
AlwI GGATC 1 cut(s) 41
AoxI GGCC 2 cut(s) 111, 296
ApeKI GCWGC 4 cut(s) 58, 61, 271, 431
AseI ATTAAT 1 cut(s) 405
Asp700I GAANNNNTTC 1 cut(s) 492
AsuHPI GGTGA 1 cut(s) 470
BalI TGGCCA 2 cut(s) 113, 298
BbsI GAAGAC 2 cut(s) 385, 515
Bbv12I GWGCWC 1 cut(s) 356
BbvI GCAGC 4 cut(s) 45, 73, 258, 418
BccI CCATC 1 cut(s) 368
BciT130I CCWGG 2 cut(s) 38, 295
BcoDI GTCTC 2 cut(s) 80, 455
BfaI CTAG 1 cut(s) 179
BfmI CTRYAG 1 cut(s) 59
BfrI CTTAAG 1 cut(s) 335
BisI GCNGC 5 cut(s) 59, 62, 272, 312, 432
BlpI GCTNAGC 1 cut(s) 250
BlsI GCNGC 5 cut(s) 60, 63, 273, 313, 433
Bme1390I CCNGG 2 cut(s) 38, 295
BmrFI CCNGG 2 cut(s) 38, 295
BmsI GCATC 2 cut(s) 67, 223
BpiI GAAGAC 2 cut(s) 385, 515
Bpu1102I GCTNAGC 1 cut(s) 250
BseBI CCWGG 2 cut(s) 38, 295
BseGI GGATG 1 cut(s) 33
BseXI GCAGC 4 cut(s) 45, 73, 258, 418
BsgI GTGCAG 1 cut(s) 257
Bsh1236I CGCG 2 cut(s) 142, 330
BshFI GGCC 2 cut(s) 113, 298
BsiHKAI GWGCWC 1 cut(s) 356
BslFI GGGAC 1 cut(s) 158
BsmAI GTCTC 2 cut(s) 80, 455
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 2 cut(s) 113, 298
Bsp1286I GDGCHC 1 cut(s) 356
Bsp143I GATC 2 cut(s) 46, 150
Bsp1720I GCTNAGC 1 cut(s) 250
BspACI CCGC 3 cut(s) 142, 311, 328
BspANI GGCC 2 cut(s) 113, 298
BspFNI CGCG 2 cut(s) 142, 330
BspHI TCATGA 1 cut(s) 153
BspMAI CTGCAG 1 cut(s) 63
BspPI GGATC 1 cut(s) 41
BspTI CTTAAG 1 cut(s) 335
BssMI GATC 2 cut(s) 46, 150
Bst2UI CCWGG 2 cut(s) 38, 295
Bst4CI ACNGT 1 cut(s) 91
Bst6I CTCTTC 2 cut(s) 393, 498
BstAFI CTTAAG 1 cut(s) 335
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 1 cut(s) 250
BstF5I GGATG 1 cut(s) 33
BstFNI CGCG 2 cut(s) 142, 330
BstKTI GATC 2 cut(s) 49, 153
BstMAI GTCTC 2 cut(s) 80, 455
BstMBI GATC 2 cut(s) 46, 150
BstMWI GCNNNNNNNGC 2 cut(s) 317, 340
BstNI CCWGG 2 cut(s) 38, 295
BstSCI CCNGG 2 cut(s) 36, 293
BstSFI CTRYAG 1 cut(s) 59
BstUI CGCG 2 cut(s) 142, 330
BstV1I GCAGC 4 cut(s) 45, 73, 258, 418
BstV2I GAAGAC 2 cut(s) 385, 515
BstXI CCANNNNNNTGG 1 cut(s) 265
BsuRI GGCC 2 cut(s) 113, 298
BtsCI GGATG 1 cut(s) 33
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 332
CciI TCATGA 1 cut(s) 153
Csp6I GTAC 2 cut(s) 466, 481
CviAII CATG 3 cut(s) 154, 265, 367
CviJI RGCY 8 cut(s) 113, 249, 254, 271, 298, 314, 334, 343
CviKI_1 RGCY 8 cut(s) 113, 249, 254, 271, 298, 314, 334, 343
CviQI GTAC 2 cut(s) 466, 481
DdeI CTNAG 1 cut(s) 250
DpnI GATC 2 cut(s) 48, 152
DpnII GATC 2 cut(s) 46, 150
DraIII CACNNNGTG 1 cut(s) 223
EaeI YGGCCR 2 cut(s) 111, 296
Eam1104I CTCTTC 2 cut(s) 393, 498
EarI CTCTTC 2 cut(s) 393, 498
EcoRII CCWGG 2 cut(s) 36, 293
FaeI CATG 3 cut(s) 157, 268, 370
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 1 cut(s) 158
FatI CATG 3 cut(s) 153, 264, 366
FauI CCCGC 2 cut(s) 135, 335
FauNDI CATATG 1 cut(s) 436
Fnu4HI GCNGC 5 cut(s) 59, 62, 272, 312, 432
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 5 cut(s) 59, 62, 272, 312, 432
FspBI CTAG 1 cut(s) 179
GluI GCNGC 5 cut(s) 59, 62, 272, 312, 432
HaeIII GGCC 2 cut(s) 113, 298
Hin1II CATG 3 cut(s) 157, 268, 370
HinfI GANTC 3 cut(s) 146, 157, 164
HphI GGTGA 1 cut(s) 470
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 1 cut(s) 100
Hpy188III TCNNGA 4 cut(s) 154, 161, 179, 218
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 1 cut(s) 238
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4V TGCA 5 cut(s) 22, 61, 274, 320, 434
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 340
HpyF3I CTNAG 1 cut(s) 250
Hsp92II CATG 3 cut(s) 157, 268, 370
Kzo9I GATC 2 cut(s) 46, 150
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 7 cut(s) 23, 50, 280, 290, 307, 396, 439
Lsp1109I GCAGC 4 cut(s) 45, 73, 258, 418
LweI GCATC 2 cut(s) 67, 223
MaeI CTAG 1 cut(s) 179
MalI GATC 2 cut(s) 48, 152
MboI GATC 2 cut(s) 46, 150
MboII GAAGA 8 cut(s) 16, 215, 279, 282, 390, 410, 485, 515
MhlI GDGCHC 1 cut(s) 356
MlsI TGGCCA 2 cut(s) 113, 298
MluCI AATT 1 cut(s) 393
MluNI TGGCCA 2 cut(s) 113, 298
MlyI GAGTC 2 cut(s) 140, 158
MnlI CCTC 4 cut(s) 127, 229, 373, 501
Mox20I TGGCCA 2 cut(s) 113, 298
MroXI GAANNNNTTC 1 cut(s) 492
MscI TGGCCA 2 cut(s) 113, 298
MseI TTAA 2 cut(s) 336, 405
MslI CAYNNNNRTG 1 cut(s) 119
Msp20I TGGCCA 2 cut(s) 113, 298
MspCI CTTAAG 1 cut(s) 335
MspR9I CCNGG 2 cut(s) 38, 295
MvaI CCWGG 2 cut(s) 38, 295
MvnI CGCG 2 cut(s) 142, 330
MwoI GCNNNNNNNGC 2 cut(s) 317, 340
NdeI CATATG 1 cut(s) 436
NdeII GATC 2 cut(s) 46, 150
NlaIII CATG 3 cut(s) 157, 268, 370
PagI TCATGA 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PdmI GAANNNNTTC 1 cut(s) 492
PfeI GAWTC 1 cut(s) 157
PkrI GCNGC 5 cut(s) 60, 63, 273, 313, 433
PleI GAGTC 2 cut(s) 140, 158
PpsI GAGTC 2 cut(s) 140, 158
PshBI ATTAAT 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 36, 293
PspGI CCWGG 2 cut(s) 36, 293
PstI CTGCAG 1 cut(s) 63
RsaI GTAC 2 cut(s) 467, 482
RsaNI GTAC 2 cut(s) 466, 481
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 2 cut(s) 336, 405
SatI GCNGC 5 cut(s) 59, 62, 272, 312, 432
Sau3AI GATC 2 cut(s) 46, 150
SchI GAGTC 2 cut(s) 140, 158
ScrFI CCNGG 2 cut(s) 38, 295
SduI GDGCHC 1 cut(s) 356
SetI ASST 6 cut(s) 256, 273, 336, 345, 385, 493
SfaNI GCATC 2 cut(s) 67, 223
SfcI CTRYAG 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 1 cut(s) 393
SsiI CCGC 3 cut(s) 142, 311, 328
SspMI CTAG 1 cut(s) 179
StyD4I CCNGG 2 cut(s) 36, 293
TaaI ACNGT 1 cut(s) 91
TaqI TCGA 2 cut(s) 149, 162
TasI AATT 1 cut(s) 393
TatI WGTACW 1 cut(s) 480
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 2 cut(s) 336, 405
Tru9I TTAA 2 cut(s) 336, 405
TscAI CASTG 1 cut(s) 96
TseI GCWGC 4 cut(s) 58, 61, 271, 431
TspDTI ATGAA 4 cut(s) 17, 186, 377, 453
TspRI CASTG 1 cut(s) 96
Vha464I CTTAAG 1 cut(s) 335
VspI ATTAAT 1 cut(s) 405
XbaI TCTAGA 1 cut(s) 178
XmnI GAANNNNTTC 1 cut(s) 492
XspI CTAG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.