RLG00000023741

CDK5RAP3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016943
Physical Location & Seq
Forward (+)
27703755 .. 27704781
1027 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023741

Sequence Viewer

Length: 384 bp
ATGATGATACGTGCACTTTGTGCTGCTGGAAAAGCCGATGTGGCAGTGGAATTTTACAAGGAAATGATGACATGCATGATGAGAGCCTCACAGATTCCTGAACACGTGGTTTATGGTTGTGTCCTGAAGTCTTTCTGCATCCCTGGCAAAATCAGAGAAGCTTTGGAATATATTCGTGACCTCAAGAATGTTATGCTTCTCCTCGAATTTCTAGACAGGCTAGTGAGTTCATTGGAAGAAAATAAGCCTCACGAAGTGAAGTTGAAAGAGGGTTGGAAGGGCTTAGCTACCAAACGTATGGAGCTGCACAACTCGTTATTCTCTTCCTGGCCAAAGCAGGAAGCTGCGCTTGCAAAAACGCGCGAGCTTAAGAAGCTGTGTTAG

Protein Analysis

128

Amino Acids

14.61

Weight (kDa)

8.99

Isoelectric Point (pI)

21.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDK5RAP3 PF05600 64 - 127 4.3e-12 CDK5 regulatory subunit-associated protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 361, 363
AcoI YGGCCR 1 cut(s) 329
AcsI RAATTY 2 cut(s) 50, 206
AcuI CTGAAG 1 cut(s) 146
AcvI CACGTG 1 cut(s) 106
AdeI CACNNNGTG 2 cut(s) 20, 256
AflII CTTAAG 1 cut(s) 368
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 1 cut(s) 265
AjnI CCWGG 2 cut(s) 142, 326
AluBI AGCT 6 cut(s) 161, 287, 304, 344, 367, 376
AluI AGCT 6 cut(s) 161, 287, 304, 344, 367, 376
Alw21I GWGCWC 1 cut(s) 16
Alw44I GTGCAC 1 cut(s) 12
AoxI GGCC 1 cut(s) 329
ApaLI GTGCAC 1 cut(s) 12
ApeKI GCWGC 3 cut(s) 23, 304, 344
ApoI RAATTY 2 cut(s) 50, 206
Asp700I GAANNNNTTC 2 cut(s) 131, 171
AspLEI GCGC 2 cut(s) 349, 363
BaeGI GKGCMC 1 cut(s) 16
BalI TGGCCA 1 cut(s) 331
BbrPI CACGTG 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 3 cut(s) 10, 291, 331
BciT130I CCWGG 2 cut(s) 144, 328
BfaI CTAG 2 cut(s) 212, 221
BfrI CTTAAG 1 cut(s) 368
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 3 cut(s) 24, 305, 345
BlpI GCTNAGC 1 cut(s) 283
BlsI GCNGC 3 cut(s) 25, 306, 346
Bme1390I CCNGG 2 cut(s) 144, 328
BmrFI CCNGG 2 cut(s) 144, 328
BmsI GCATC 1 cut(s) 147
Bpu1102I GCTNAGC 1 cut(s) 283
BpuEI CTTGAG 1 cut(s) 167
BsaAI YACGTR 2 cut(s) 11, 106
BsaJI CCNNGG 1 cut(s) 142
BseBI CCWGG 2 cut(s) 144, 328
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 1 cut(s) 138
BseRI GAGGAG 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 16
BseXI GCAGC 3 cut(s) 10, 291, 331
BsgI GTGCAG 1 cut(s) 290
Bsh1236I CGCG 2 cut(s) 361, 363
BshFI GGCC 1 cut(s) 331
BsiHKAI GWGCWC 1 cut(s) 16
BsnI GGCC 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 16
Bsp1720I GCTNAGC 1 cut(s) 283
BspANI GGCC 1 cut(s) 331
BspFNI CGCG 2 cut(s) 361, 363
BspTI CTTAAG 1 cut(s) 368
BssECI CCNNGG 1 cut(s) 142
Bst2UI CCWGG 2 cut(s) 144, 328
Bst6I CTCTTC 1 cut(s) 328
BstAFI CTTAAG 1 cut(s) 368
BstAPI GCANNNNNTGC 1 cut(s) 20
BstBAI YACGTR 2 cut(s) 11, 106
BstC8I GCNNGC 2 cut(s) 351, 365
BstDEI CTNAG 1 cut(s) 283
BstF5I GGATG 1 cut(s) 138
BstFNI CGCG 2 cut(s) 361, 363
BstHHI GCGC 2 cut(s) 349, 363
BstMWI GCNNNNNNNGC 6 cut(s) 20, 32, 41, 144, 350, 373
BstNI CCWGG 2 cut(s) 144, 328
BstNSI RCATGY 1 cut(s) 75
BstSCI CCNGG 2 cut(s) 142, 326
BstSLI GKGCMC 1 cut(s) 16
BstUI CGCG 2 cut(s) 361, 363
BstV1I GCAGC 3 cut(s) 10, 291, 331
BstXI CCANNNNNNTGG 1 cut(s) 298
BsuRI GGCC 1 cut(s) 331
BtsCI GGATG 1 cut(s) 138
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 51
Cac8I GCNNGC 2 cut(s) 351, 365
CfoI GCGC 2 cut(s) 349, 363
CviAII CATG 2 cut(s) 72, 76
DdeI CTNAG 1 cut(s) 283
DraIII CACNNNGTG 2 cut(s) 20, 256
EaeI YGGCCR 1 cut(s) 329
Eam1104I CTCTTC 1 cut(s) 328
EarI CTCTTC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 146
Eco72I CACGTG 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 142, 326
EcoT22I ATGCAT 1 cut(s) 77
FaeI CATG 2 cut(s) 75, 79
FaiI YATR 6 cut(s) 73, 77, 114, 171, 194, 299
FalI AAGNNNNNCTT 2 cut(s) 333, 365
FatI CATG 2 cut(s) 71, 75
Fnu4HI GCNGC 3 cut(s) 24, 305, 345
FokI GGATG 1 cut(s) 125
Fsp4HI GCNGC 3 cut(s) 24, 305, 345
FspBI CTAG 2 cut(s) 212, 221
GlaI GCGC 2 cut(s) 348, 362
GluI GCNGC 3 cut(s) 24, 305, 345
HaeIII GGCC 1 cut(s) 331
HhaI GCGC 2 cut(s) 349, 363
Hin1II CATG 2 cut(s) 75, 79
Hin6I GCGC 2 cut(s) 347, 361
HinP1I GCGC 2 cut(s) 347, 361
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 14
Hpy188I TCNGA 1 cut(s) 155
Hpy188III TCNNGA 6 cut(s) 98, 124, 176, 184, 212, 251
Hpy8I GTNNAC 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 271
HpyCH4IV ACGT 3 cut(s) 10, 105, 295
HpyCH4V TGCA 5 cut(s) 14, 75, 138, 307, 353
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 32, 41, 144, 350, 373
HpyF3I CTNAG 1 cut(s) 283
HpySE526I ACGT 3 cut(s) 10, 105, 295
Hsp92II CATG 2 cut(s) 75, 79
HspAI GCGC 2 cut(s) 347, 361
LmnI GCTCC 1 cut(s) 301
LpnPI CCDG 9 cut(s) 12, 111, 129, 137, 156, 202, 313, 323, 340
Lsp1109I GCAGC 3 cut(s) 10, 291, 331
LweI GCATC 1 cut(s) 147
MaeI CTAG 2 cut(s) 212, 221
MaeII ACGT 3 cut(s) 10, 105, 295
MaeIII GTNAC 1 cut(s) 176
MboII GAAGA 2 cut(s) 248, 315
MhlI GDGCHC 1 cut(s) 16
MlsI TGGCCA 1 cut(s) 331
MluCI AATT 2 cut(s) 50, 206
MluNI TGGCCA 1 cut(s) 331
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 5 cut(s) 97, 191, 212, 258, 262
Mox20I TGGCCA 1 cut(s) 331
Mph1103I ATGCAT 1 cut(s) 77
MroXI GAANNNNTTC 2 cut(s) 131, 171
MscI TGGCCA 1 cut(s) 331
MseI TTAA 1 cut(s) 369
Msp20I TGGCCA 1 cut(s) 331
MspCI CTTAAG 1 cut(s) 368
MspR9I CCNGG 2 cut(s) 144, 328
MvaI CCWGG 2 cut(s) 144, 328
MvnI CGCG 2 cut(s) 361, 363
MwoI GCNNNNNNNGC 6 cut(s) 20, 32, 41, 144, 350, 373
NlaIII CATG 2 cut(s) 75, 79
NmuCI GTSAC 1 cut(s) 176
NsiI ATGCAT 1 cut(s) 77
NspI RCATGY 1 cut(s) 75
PdmI GAANNNNTTC 2 cut(s) 131, 171
PfeI GAWTC 1 cut(s) 94
PkrI GCNGC 3 cut(s) 25, 306, 346
PmaCI CACGTG 1 cut(s) 106
PmlI CACGTG 1 cut(s) 106
Ppu21I YACGTR 2 cut(s) 11, 106
Psp6I CCWGG 2 cut(s) 142, 326
PspCI CACGTG 1 cut(s) 106
PspGI CCWGG 2 cut(s) 142, 326
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 3 cut(s) 24, 305, 345
ScrFI CCNGG 2 cut(s) 144, 328
SduI GDGCHC 1 cut(s) 16
SfaNI GCATC 1 cut(s) 147
SmlI CTYRAG 2 cut(s) 182, 368
SmoI CTYRAG 2 cut(s) 182, 368
Sse9I AATT 2 cut(s) 50, 206
SspMI CTAG 2 cut(s) 212, 221
StyD4I CCNGG 2 cut(s) 142, 326
TaiI ACGT 3 cut(s) 13, 108, 298
TaqI TCGA 1 cut(s) 204
TasI AATT 2 cut(s) 50, 206
TfiI GAWTC 1 cut(s) 94
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 1 cut(s) 176
TseI GCWGC 3 cut(s) 23, 304, 344
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 1 cut(s) 219
TspRI CASTG 1 cut(s) 51
Vha464I CTTAAG 1 cut(s) 368
VneI GTGCAC 1 cut(s) 12
XapI RAATTY 2 cut(s) 50, 206
XbaI TCTAGA 1 cut(s) 211
XceI RCATGY 1 cut(s) 75
XmnI GAANNNNTTC 2 cut(s) 131, 171
XspI CTAG 2 cut(s) 212, 221
Zsp2I ATGCAT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.