Rh3BG235500

CDK5RAP3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0016943
Physical Location & Seq
Reverse (-)
22054119 .. 22055210
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG235500.1

Sequence Viewer

Length: 348 bp
ATGATTCTCAACTCCAAAAGATTTCTAGACAGACTAATGAGTTCATTGGAAGAAAAGAAGCATCACGAAGTGAAGTTGAAAGAGGGTTTGAAGGGCTTAGCTACCAAACGCATGGAGCTGCACAACTCGTTATCTTCTTCCTGGCCAAAGCAGGAAGCGGCTCTTGCAAAAACCTGCGAGCTTAAGAAGCTGTGTGAGAGCACCCTTTCCTCCATGTTTGATGGAAGGCCTGTTCATGAAGAGATTAATGCCCGTTTGAATAGTGGTGTTGCTGCATATGAAAATGTTTGTCTCTGGCGTGAAGCGTACAATATGAGCGTGTACTTTGAATCTCTTCGTTTTAACTAA

Protein Analysis

115

Amino Acids

13.28

Weight (kDa)

8.81

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDK5RAP3 PF05600 1 - 79 2.1e-23 CDK5 regulatory subunit-associated protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 182
AciI CCGC 1 cut(s) 158
AcoI YGGCCR 1 cut(s) 143
AdeI CACNNNGTG 1 cut(s) 70
AfaI GTAC 2 cut(s) 308, 323
AflII CTTAAG 1 cut(s) 182
AgsI TTSAA 4 cut(s) 79, 91, 259, 329
AjnI CCWGG 1 cut(s) 140
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
AluBI AGCT 4 cut(s) 101, 118, 181, 190
AluI AGCT 4 cut(s) 101, 118, 181, 190
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 1 cut(s) 296
AoxI GGCC 2 cut(s) 143, 227
ApeKI GCWGC 2 cut(s) 118, 272
AseI ATTAAT 1 cut(s) 246
Asp700I GAANNNNTTC 1 cut(s) 333
BalI TGGCCA 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 2 cut(s) 105, 259
BccI CCATC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 1 cut(s) 26
BfrI CTTAAG 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 182
BisI GCNGC 3 cut(s) 119, 159, 273
BlpI GCTNAGC 1 cut(s) 97
BlsI GCNGC 3 cut(s) 120, 160, 274
Bme1390I CCNGG 1 cut(s) 142
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 70
Bpu1102I GCTNAGC 1 cut(s) 97
BseBI CCWGG 1 cut(s) 142
BseXI GCAGC 2 cut(s) 105, 259
BsgI GTGCAG 1 cut(s) 104
BshFI GGCC 2 cut(s) 145, 229
BsiHKAI GWGCWC 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 296
BsnI GGCC 2 cut(s) 145, 229
Bsp1286I GDGCHC 1 cut(s) 203
Bsp1720I GCTNAGC 1 cut(s) 97
BspACI CCGC 1 cut(s) 158
BspANI GGCC 2 cut(s) 145, 229
BspHI TCATGA 1 cut(s) 235
BspMI ACCTGC 1 cut(s) 182
BspTI CTTAAG 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 142
Bst6I CTCTTC 2 cut(s) 234, 339
BstAFI CTTAAG 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 179
BstDEI CTNAG 1 cut(s) 97
BstMAI GTCTC 1 cut(s) 296
BstMWI GCNNNNNNNGC 2 cut(s) 164, 187
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BstV1I GCAGC 2 cut(s) 105, 259
BstXI CCANNNNNNTGG 1 cut(s) 112
BsuRI GGCC 2 cut(s) 145, 229
BveI ACCTGC 1 cut(s) 182
Cac8I GCNNGC 1 cut(s) 179
CciI TCATGA 1 cut(s) 235
Csp6I GTAC 2 cut(s) 307, 322
CviAII CATG 3 cut(s) 112, 214, 236
CviJI RGCY 8 cut(s) 96, 101, 118, 145, 161, 181, 190, 229
CviKI_1 RGCY 8 cut(s) 96, 101, 118, 145, 161, 181, 190, 229
CviQI GTAC 2 cut(s) 307, 322
DdeI CTNAG 1 cut(s) 97
DraIII CACNNNGTG 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 143
Eam1104I CTCTTC 2 cut(s) 234, 339
EarI CTCTTC 2 cut(s) 234, 339
Eco147I AGGCCT 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 140
FaeI CATG 3 cut(s) 115, 217, 239
FaiI YATR 6 cut(s) 113, 215, 237, 277, 279, 314
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FatI CATG 3 cut(s) 111, 213, 235
FauNDI CATATG 1 cut(s) 277
Fnu4HI GCNGC 3 cut(s) 119, 159, 273
Fsp4HI GCNGC 3 cut(s) 119, 159, 273
FspBI CTAG 1 cut(s) 26
GluI GCNGC 3 cut(s) 119, 159, 273
HaeIII GGCC 2 cut(s) 145, 229
Hin1II CATG 3 cut(s) 115, 217, 239
HinfI GANTC 2 cut(s) 4, 329
Hpy166II GTNNAC 1 cut(s) 322
Hpy188III TCNNGA 3 cut(s) 26, 65, 236
Hpy8I GTNNAC 1 cut(s) 322
HpyAV CCTTC 2 cut(s) 85, 219
HpyCH4V TGCA 3 cut(s) 121, 167, 275
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 187
HpyF3I CTNAG 1 cut(s) 97
Hsp92II CATG 3 cut(s) 115, 217, 239
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 6 cut(s) 127, 137, 154, 187, 243, 280
Lsp1109I GCAGC 2 cut(s) 105, 259
LweI GCATC 1 cut(s) 70
MaeI CTAG 1 cut(s) 26
MboII GAAGA 5 cut(s) 62, 126, 129, 251, 326
MhlI GDGCHC 1 cut(s) 203
MlsI TGGCCA 1 cut(s) 145
MluNI TGGCCA 1 cut(s) 145
MnlI CCTC 2 cut(s) 76, 220
Mox20I TGGCCA 1 cut(s) 145
MroXI GAANNNNTTC 1 cut(s) 333
MscI TGGCCA 1 cut(s) 145
MseI TTAA 3 cut(s) 183, 246, 342
Msp20I TGGCCA 1 cut(s) 145
MspCI CTTAAG 1 cut(s) 182
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
MwoI GCNNNNNNNGC 2 cut(s) 164, 187
NdeI CATATG 1 cut(s) 277
NlaIII CATG 3 cut(s) 115, 217, 239
PagI TCATGA 1 cut(s) 235
PceI AGGCCT 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 4, 329
PkrI GCNGC 3 cut(s) 120, 160, 274
PshBI ATTAAT 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
RsaI GTAC 2 cut(s) 308, 323
RsaNI GTAC 2 cut(s) 307, 322
SaqAI TTAA 3 cut(s) 183, 246, 342
SatI GCNGC 3 cut(s) 119, 159, 273
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 1 cut(s) 203
SetI ASST 5 cut(s) 103, 120, 176, 183, 192
SfaNI GCATC 1 cut(s) 70
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SseBI AGGCCT 1 cut(s) 229
SsiI CCGC 1 cut(s) 158
SspMI CTAG 1 cut(s) 26
StuI AGGCCT 1 cut(s) 229
StyD4I CCNGG 1 cut(s) 140
TatI WGTACW 1 cut(s) 321
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 2 cut(s) 4, 329
Tru1I TTAA 3 cut(s) 183, 246, 342
Tru9I TTAA 3 cut(s) 183, 246, 342
TseI GCWGC 2 cut(s) 118, 272
TspDTI ATGAA 4 cut(s) 33, 224, 252, 294
Vha464I CTTAAG 1 cut(s) 182
VspI ATTAAT 1 cut(s) 246
XbaI TCTAGA 1 cut(s) 25
XmnI GAANNNNTTC 1 cut(s) 333
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.