RchiOBHm_Chr3g0485041

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008108
Physical Location & Seq
Forward (+)
31624282 .. 31626797
2516 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44971

Sequence Viewer

Length: 1092 bp
ATGCAAATGGTTGCAGATAGTTTCTGGAGCTCGAAAACCAAGGCAAAGCAGGAGTCTTGTCTCAGATTGAAAGGAGCCGACTGTCTTTTTCATCCTTTTTTTCATCAAATTTCTTATCTTTACCTCCATCTTATTCAAATCCATTACAGCCCCATGACTGATTTCACCCTTATTGATTCTGAGACCGAGATCGAAGCCTCTCGAGGTACCAGTAGAACAAGAGTGAATATGGCTGAGGGATCTTCAATTGAAGGCCCCCAGGGGAAGAGGTTGCTGCAGCACCTTAAAGACCTACATGAGGAAAACAAGACACTCATGAAGCTCGTTGCGGACAAAGAAAAGCGTATTGAATCGTACATGATAGATTTTGAAGAGCTGAATGGCTTGAAAATCATTGTCAATAAACTAGAGAAACAAAATTTCGTACTCACCCAAGCAAACAGCCAGATGTTGCAAGAGCTACGGAGTTTGAGAAACCATGTGCAGAAAGTAGAACAACCAACTTTACCACCCGCCCAAGAAATAAACCAGGTGCAGGAAGCACAACAACCAAATTTGCAACCTGCCCAAGCAAGAGTCCAGGTGCAGGAATTAGAACCACCAAGTTTGCAAGCTGCCCAAGCAAGTAGCCAGATGCAGGAAGAAGAACTTGACCTAGAAAATAGCCAGATATTGCTGGAACTCAAATCACATGATCATATGTTAAGTTCTACTGACGAAGCAGTCCGGGAGTGTATAGATATGATCGAAAAACTTCAAGCTTTGGGCCCTCCTACTAATGAAGCAATCCAACAAGTTAAGGCAAAGCTTCAAGCTTTGGGCCCTCTTACTATTGATGCAGTCCGGCAGATTAGAGTGGGGTTTCGTAAGCTCCAGAAATTGCAGTGTCCAAGGTGTGAATCTAATAACACTAAATTCAGTGTTTTGAAAGCCAAAAGAATATCTGCCCCAAGGTATCACTGCAAAAATTGTAAAATGGAATGGACATTAGGGGGCGAAATAAGGAAACCTATTGTGCTTGGAGGCGTGAATAGAAGAGATCTTCTTCAGCAGCGCATCCAACGTGTTCAGCGTAGAAGGATGAAGACTTGA

Protein Analysis

363

Amino Acids

41.74

Weight (kDa)

8.96

Isoelectric Point (pI)

61.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 293 - 346 9.2e-17 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 722
Acc36I ACCTGC 1 cut(s) 571
Acc65I GGTACC 1 cut(s) 206
AccB1I GGYRCC 1 cut(s) 206
AciI CCGC 2 cut(s) 329, 513
AclWI GGATC 1 cut(s) 247
AcsI RAATTY 4 cut(s) 108, 418, 553, 914
AcuI CTGAAG 1 cut(s) 1031
AfaI GTAC 3 cut(s) 208, 356, 426
AfiI CCNNNNNNNGG 4 cut(s) 298, 535, 586, 637
AflIII ACRYGT 1 cut(s) 1063
AjnI CCWGG 3 cut(s) 258, 528, 579
AluBI AGCT 9 cut(s) 30, 322, 376, 460, 614, 761, 808, 815, 871
AluI AGCT 9 cut(s) 30, 322, 376, 460, 614, 761, 808, 815, 871
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 65, 176
AlwI GGATC 1 cut(s) 247
Ama87I CYCGRG 1 cut(s) 201
AoxI GGCC 3 cut(s) 253, 766, 820
ApaI GGGCCC 2 cut(s) 770, 824
ApeKI GCWGC 4 cut(s) 274, 277, 614, 1051
ApoI RAATTY 4 cut(s) 108, 418, 553, 914
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
Asp700I GAANNNNTTC 1 cut(s) 753
Asp718I GGTACC 1 cut(s) 206
AspLEI GCGC 1 cut(s) 1056
AspS9I GGNCC 5 cut(s) 254, 766, 767, 820, 821
AsuC2I CCSGG 1 cut(s) 728
AsuHPI GGTGA 2 cut(s) 157, 421
AvaI CYCGRG 1 cut(s) 201
BaeGI GKGCMC 2 cut(s) 770, 824
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 3 cut(s) 32, 770, 824
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 4 cut(s) 261, 289, 601, 1063
BccI CCATC 1 cut(s) 135
BciT130I CCWGG 3 cut(s) 260, 530, 581
BclI TGATCA 1 cut(s) 694
BcnI CCSGG 1 cut(s) 728
BcoDI GTCTC 2 cut(s) 65, 176
BfaI CTAG 2 cut(s) 407, 656
BfmI CTRYAG 1 cut(s) 275
BfuAI ACCTGC 1 cut(s) 571
BglII AGATCT 1 cut(s) 1039
BisI GCNGC 4 cut(s) 275, 278, 615, 1052
BlsI GCNGC 4 cut(s) 276, 279, 616, 1053
Bme1390I CCNGG 4 cut(s) 260, 530, 581, 728
BmeT110I CYCGRG 1 cut(s) 201
BmgT120I GGNCC 5 cut(s) 254, 766, 767, 820, 821
BmiI GGNNCC 5 cut(s) 76, 208, 256, 768, 822
BmrFI CCNGG 4 cut(s) 260, 530, 581, 728
BmsI GCATC 3 cut(s) 624, 826, 1065
BpmI CTGGAG 2 cut(s) 46, 857
Bpu10I CCTNAGC 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 728
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 5 cut(s) 39, 258, 259, 890, 950
Bsc4I CCNNNNNNNGG 4 cut(s) 298, 535, 586, 637
Bse1I ACTGG 1 cut(s) 210
BseBI CCWGG 3 cut(s) 260, 530, 581
BseDI CCNNGG 5 cut(s) 39, 258, 259, 890, 950
BseGI GGATG 3 cut(s) 91, 1056, 1086
BseLI CCNNNNNNNGG 4 cut(s) 298, 535, 586, 637
BseMII CTCAG 3 cut(s) 76, 171, 225
BseNI ACTGG 1 cut(s) 210
BseSI GKGCMC 2 cut(s) 770, 824
BseXI GCAGC 4 cut(s) 261, 289, 601, 1063
BsgI GTGCAG 3 cut(s) 503, 554, 605
BshFI GGCC 3 cut(s) 255, 768, 822
BshNI GGYRCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 201
BsiSI CCGG 2 cut(s) 727, 844
BslI CCNNNNNNNGG 4 cut(s) 298, 535, 586, 637
BsmAI GTCTC 2 cut(s) 65, 176
BsnI GGCC 3 cut(s) 255, 768, 822
Bso31I GGTCTC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 201
Bsp120I GGGCCC 2 cut(s) 766, 820
Bsp1286I GDGCHC 3 cut(s) 32, 770, 824
Bsp143I GATC 5 cut(s) 189, 239, 694, 744, 1039
BspACI CCGC 2 cut(s) 329, 513
BspANI GGCC 3 cut(s) 255, 768, 822
BspCNI CTCAG 3 cut(s) 75, 172, 226
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 5 cut(s) 76, 208, 256, 768, 822
BspMAI CTGCAG 1 cut(s) 279
BspMI ACCTGC 1 cut(s) 571
BspPI GGATC 1 cut(s) 247
BspQI GCTCTTC 1 cut(s) 366
BspT107I GGYRCC 1 cut(s) 206
BspTNI GGTCTC 1 cut(s) 176
BsrI ACTGG 1 cut(s) 210
BssECI CCNNGG 5 cut(s) 39, 258, 259, 890, 950
BssMI GATC 5 cut(s) 189, 239, 694, 744, 1039
BssT1I CCWWGG 3 cut(s) 39, 890, 950
Bst2UI CCWGG 3 cut(s) 260, 530, 581
Bst4CI ACNGT 1 cut(s) 83
Bst6I CTCTTC 3 cut(s) 260, 366, 1030
BstC8I GCNNGC 1 cut(s) 612
BstDEI CTNAG 3 cut(s) 62, 180, 234
BstENI CCTNNNNNAGG 1 cut(s) 296
BstF5I GGATG 3 cut(s) 91, 1056, 1086
BstHHI GCGC 1 cut(s) 1056
BstKTI GATC 5 cut(s) 192, 242, 697, 747, 1042
BstMAI GTCTC 2 cut(s) 65, 176
BstMBI GATC 5 cut(s) 189, 239, 694, 744, 1039
BstMWI GCNNNNNNNGC 1 cut(s) 620
BstNI CCWGG 3 cut(s) 260, 530, 581
BstSCI CCNGG 4 cut(s) 258, 528, 579, 726
BstSFI CTRYAG 1 cut(s) 275
BstSLI GKGCMC 2 cut(s) 770, 824
BstV1I GCAGC 4 cut(s) 261, 289, 601, 1063
BstX2I RGATCY 2 cut(s) 239, 1039
BstYI RGATCY 2 cut(s) 239, 1039
BsuRI GGCC 3 cut(s) 255, 768, 822
BtsCI GGATG 3 cut(s) 91, 1056, 1086
BtsI GCAGTG 2 cut(s) 890, 958
BtsIMutI CAGTG 3 cut(s) 890, 925, 958
BveI ACCTGC 1 cut(s) 571
Cac8I GCNNGC 1 cut(s) 612
CciI TCATGA 1 cut(s) 315
CfoI GCGC 1 cut(s) 1056
Cfr13I GGNCC 5 cut(s) 254, 766, 767, 820, 821
CsiI ACCWGGT 1 cut(s) 528
Csp6I GTAC 3 cut(s) 207, 355, 425
CspCI CAANNNNNGTGG 2 cut(s) 588, 623
CviAII CATG 6 cut(s) 154, 296, 316, 358, 479, 692
CviQI GTAC 3 cut(s) 207, 355, 425
DdeI CTNAG 3 cut(s) 62, 180, 234
DpnI GATC 5 cut(s) 191, 241, 696, 746, 1041
DpnII GATC 5 cut(s) 189, 239, 694, 744, 1039
DrdI GACNNNNNNGTC 1 cut(s) 722
DseDI GACNNNNNNGTC 1 cut(s) 722
Eam1104I CTCTTC 3 cut(s) 260, 366, 1030
EarI CTCTTC 3 cut(s) 260, 366, 1030
Ecl136II GAGCTC 1 cut(s) 30
Eco130I CCWWGG 3 cut(s) 39, 890, 950
Eco24I GRGCYC 3 cut(s) 32, 770, 824
Eco31I GGTCTC 1 cut(s) 176
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 1031
Eco88I CYCGRG 1 cut(s) 201
EcoICRI GAGCTC 1 cut(s) 30
EcoNI CCTNNNNNAGG 1 cut(s) 296
EcoO109I RGGNCCY 3 cut(s) 254, 767, 821
EcoRII CCWGG 3 cut(s) 258, 528, 579
EcoT14I CCWWGG 3 cut(s) 39, 890, 950
EcoT38I GRGCYC 3 cut(s) 32, 770, 824
ErhI CCWWGG 3 cut(s) 39, 890, 950
FaeI CATG 6 cut(s) 157, 299, 319, 361, 482, 695
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FatI CATG 6 cut(s) 153, 295, 315, 357, 478, 691
FauI CCCGC 1 cut(s) 520
FauNDI CATATG 1 cut(s) 699
FbaI TGATCA 1 cut(s) 694
Fnu4HI GCNGC 4 cut(s) 275, 278, 615, 1052
FokI GGATG 2 cut(s) 78, 1043
FriOI GRGCYC 3 cut(s) 32, 770, 824
Fsp4HI GCNGC 4 cut(s) 275, 278, 615, 1052
FspBI CTAG 2 cut(s) 407, 656
GlaI GCGC 1 cut(s) 1055
GluI GCNGC 4 cut(s) 275, 278, 615, 1052
GsuI CTGGAG 2 cut(s) 46, 857
HaeIII GGCC 3 cut(s) 255, 768, 822
HapII CCGG 2 cut(s) 727, 844
HhaI GCGC 1 cut(s) 1056
Hin1II CATG 6 cut(s) 157, 299, 319, 361, 482, 695
Hin6I GCGC 1 cut(s) 1054
HinP1I GCGC 1 cut(s) 1054
HindIII AAGCTT 3 cut(s) 759, 806, 813
HinfI GANTC 5 cut(s) 53, 176, 350, 576, 899
HpaII CCGG 2 cut(s) 727, 844
HphI GGTGA 2 cut(s) 157, 421
Hpy188I TCNGA 2 cut(s) 65, 181
Hpy188III TCNNGA 4 cut(s) 25, 201, 316, 874
HpyAV CCTTC 2 cut(s) 245, 1071
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 1 cut(s) 1063
HpyF10VI GCNNNNNNNGC 1 cut(s) 620
HpyF3I CTNAG 3 cut(s) 62, 180, 234
HpySE526I ACGT 1 cut(s) 1063
Hsp92II CATG 6 cut(s) 157, 299, 319, 361, 482, 695
HspAI GCGC 1 cut(s) 1054
KpnI GGTACC 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 694
Kzo9I GATC 5 cut(s) 189, 239, 694, 744, 1039
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 3 cut(s) 27, 74, 876
Lsp1109I GCAGC 4 cut(s) 261, 289, 601, 1063
LweI GCATC 3 cut(s) 624, 826, 1065
MabI ACCWGGT 1 cut(s) 528
MaeI CTAG 2 cut(s) 407, 656
MaeII ACGT 1 cut(s) 1063
MalI GATC 5 cut(s) 191, 241, 696, 746, 1041
MboI GATC 5 cut(s) 189, 239, 694, 744, 1039
MboII GAAGA 8 cut(s) 234, 277, 383, 653, 656, 1034, 1037, 1047
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 2 cut(s) 239, 1039
MhlI GDGCHC 3 cut(s) 32, 770, 824
MluCI AATT 8 cut(s) 108, 246, 418, 553, 590, 878, 914, 967
MlyI GAGTC 2 cut(s) 62, 585
MmeI TCCRAC 2 cut(s) 814, 1084
MnlI CCTC 9 cut(s) 134, 197, 208, 229, 261, 292, 780, 834, 1016
MroXI GAANNNNTTC 1 cut(s) 753
MseI TTAA 3 cut(s) 285, 704, 798
MspI CCGG 2 cut(s) 727, 844
MspR9I CCNGG 4 cut(s) 260, 530, 581, 728
MunI CAATTG 1 cut(s) 246
MvaI CCWGG 3 cut(s) 260, 530, 581
MwoI GCNNNNNNNGC 1 cut(s) 620
NciI CCSGG 1 cut(s) 728
NdeI CATATG 1 cut(s) 699
NdeII GATC 5 cut(s) 189, 239, 694, 744, 1039
NlaIII CATG 6 cut(s) 157, 299, 319, 361, 482, 695
NlaIV GGNNCC 5 cut(s) 76, 208, 256, 768, 822
PaeR7I CTCGAG 1 cut(s) 201
PagI TCATGA 1 cut(s) 315
PasI CCCWGGG 1 cut(s) 259
PciSI GCTCTTC 1 cut(s) 366
PcsI WCGNNNNNNNCGW 1 cut(s) 1069
PdmI GAANNNNTTC 1 cut(s) 753
PfeI GAWTC 3 cut(s) 176, 350, 899
PfoI TCCNGGA 1 cut(s) 726
PkrI GCNGC 4 cut(s) 276, 279, 616, 1053
PleI GAGTC 2 cut(s) 61, 584
PpsI GAGTC 2 cut(s) 61, 584
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 3 cut(s) 258, 528, 579
PspGI CCWGG 3 cut(s) 258, 528, 579
PspN4I GGNNCC 5 cut(s) 76, 208, 256, 768, 822
PspOMI GGGCCC 2 cut(s) 766, 820
PspPI GGNCC 5 cut(s) 254, 766, 767, 820, 821
PstI CTGCAG 1 cut(s) 279
PsuI RGATCY 2 cut(s) 239, 1039
RsaI GTAC 3 cut(s) 208, 356, 426
RsaNI GTAC 3 cut(s) 207, 355, 425
SacI GAGCTC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 3 cut(s) 285, 704, 798
SatI GCNGC 4 cut(s) 275, 278, 615, 1052
Sau3AI GATC 5 cut(s) 189, 239, 694, 744, 1039
Sau96I GGNCC 5 cut(s) 254, 766, 767, 820, 821
SchI GAGTC 2 cut(s) 62, 585
ScrFI CCNGG 4 cut(s) 260, 530, 581, 728
SduI GDGCHC 3 cut(s) 32, 770, 824
SexAI ACCWGGT 1 cut(s) 528
SfaNI GCATC 3 cut(s) 624, 826, 1065
SfcI CTRYAG 1 cut(s) 275
Sfr274I CTCGAG 1 cut(s) 201
SlaI CTCGAG 1 cut(s) 201
SmlI CTYRAG 1 cut(s) 201
SmoI CTYRAG 1 cut(s) 201
Sse9I AATT 8 cut(s) 108, 246, 418, 553, 590, 878, 914, 967
SsiI CCGC 2 cut(s) 329, 513
SspMI CTAG 2 cut(s) 407, 656
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 4 cut(s) 258, 528, 579, 726
StyI CCWWGG 3 cut(s) 39, 890, 950
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 1 cut(s) 1066
TaqI TCGA 4 cut(s) 32, 192, 202, 747
TaqII GACCGA 1 cut(s) 200
TasI AATT 8 cut(s) 108, 246, 418, 553, 590, 878, 914, 967
TfiI GAWTC 3 cut(s) 176, 350, 899
Tru1I TTAA 3 cut(s) 285, 704, 798
Tru9I TTAA 3 cut(s) 285, 704, 798
TscAI CASTG 3 cut(s) 890, 925, 965
TseI GCWGC 4 cut(s) 274, 277, 614, 1051
TspDTI ATGAA 4 cut(s) 80, 92, 332, 795
TspGWI ACGGA 1 cut(s) 478
TspRI CASTG 3 cut(s) 890, 925, 965
XagI CCTNNNNNAGG 1 cut(s) 296
XapI RAATTY 4 cut(s) 108, 418, 553, 914
XhoI CTCGAG 1 cut(s) 201
XmnI GAANNNNTTC 1 cut(s) 753
XspI CTAG 2 cut(s) 407, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.