Rh3DG295500

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0008108
Physical Location & Seq
Forward (+)
30723218 .. 30725748
2531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG295500.1

Sequence Viewer

Length: 912 bp
ATGGCTGAGGGATCTTCAATTGAAGGCCCCCAGGGGAAGAGGTTGCTGCAGCACCTTAAAGACCTACACGAGGAAAACAAGACGCTCATGAAGCTCGTTGCAGACAAAGAAAAGCGTATTGAATCGTACATGATAGATTTTGAAGAGCTGAATGGCTTGAAAATCATTGTCAATAAACTAGAGAAACAAAATTTCATACTCACCCAAGCAAACAGCCAGATGTTGCAAGAGCTACGGAGTTTGAGAAACCATGTGCAGAAAGTAGAACAACCAAATTTAACACCCGCCCAAGAAATAAACCAGGTGCACGAAGTACAACAACCAAATTTGCAACCCCAAGAAATAATCCAGGTGCAGGAAGCACAACAACCAAATTTGCAACCTGCCCAAGCAAGAGTCCAGGTGCAGCAATTAGAACCACCAAGTTTGCAAGCTGCCCAAGCAAGTAGCCAGATGCAGGAAGAAGAACTTGACCTAGAAAATAGCCAGATATTGCTGGAACTCAAATCACATGATCATATGTTAAGTTCTACTGACGAAGCAGTCCAGGAGTGTATAGATATGATCGAAAAACTTCAAGCTTTGGGCCCTCCTACTAATGAAGCAATCCAACAAGTTAAGGCAAAGCTTCAAGCTTTGGGCCCTCTTACTATTGATGCAGTCCGGCAGATTAGAGTGGGGTTTCGTAAGCTTCAGAAATTGCAGTGTCCAAGGTGTGAATCTAATAACACTAAATTCAGTGTTTTGAAAGCCAAAAGAATATCTGCCCCAAGGTATCACTGCAAAAATTGTAAAATGGAATGGACATTAGGGGGCGAAATAAGGAAACCTATTGTGCTTGGAGGCGTGAATAGAAGAGATCTTCTTCAGCAGCGCATCCAACGTGTTCAGCGTAGAAGGATGAAGACTTGA

Protein Analysis

303

Amino Acids

34.78

Weight (kDa)

8.89

Isoelectric Point (pI)

63.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 233 - 286 7e-17 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 542
Acc36I ACCTGC 1 cut(s) 391
AciI CCGC 1 cut(s) 285
AclWI GGATC 1 cut(s) 19
AcsI RAATTY 5 cut(s) 190, 274, 325, 373, 734
AcuI CTGAAG 2 cut(s) 677, 851
AfaI GTAC 2 cut(s) 128, 315
AfiI CCNNNNNNNGG 3 cut(s) 70, 355, 457
AflIII ACRYGT 1 cut(s) 883
AgsI TTSAA 8 cut(s) 18, 23, 122, 143, 160, 578, 632, 748
AjnI CCWGG 5 cut(s) 30, 300, 348, 399, 546
AluBI AGCT 8 cut(s) 94, 148, 232, 434, 581, 628, 635, 691
AluI AGCT 8 cut(s) 94, 148, 232, 434, 581, 628, 635, 691
Alw21I GWGCWC 1 cut(s) 309
Alw44I GTGCAC 1 cut(s) 305
AlwI GGATC 1 cut(s) 19
AoxI GGCC 3 cut(s) 25, 586, 640
ApaI GGGCCC 2 cut(s) 590, 644
ApaLI GTGCAC 1 cut(s) 305
ApeKI GCWGC 5 cut(s) 46, 49, 406, 434, 871
ApoI RAATTY 5 cut(s) 190, 274, 325, 373, 734
Asp700I GAANNNNTTC 1 cut(s) 573
AspLEI GCGC 1 cut(s) 876
AspS9I GGNCC 5 cut(s) 26, 586, 587, 640, 641
AsuHPI GGTGA 1 cut(s) 193
BaeGI GKGCMC 3 cut(s) 309, 590, 644
BanII GRGCYC 2 cut(s) 590, 644
BauI CACGAG 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 5 cut(s) 33, 61, 418, 421, 883
BciT130I CCWGG 5 cut(s) 32, 302, 350, 401, 548
BclI TGATCA 1 cut(s) 514
BfaI CTAG 2 cut(s) 179, 476
BfmI CTRYAG 1 cut(s) 47
BfuAI ACCTGC 1 cut(s) 391
BglII AGATCT 1 cut(s) 859
BisI GCNGC 5 cut(s) 47, 50, 407, 435, 872
BlsI GCNGC 5 cut(s) 48, 51, 408, 436, 873
Bme1390I CCNGG 5 cut(s) 32, 302, 350, 401, 548
BmgT120I GGNCC 5 cut(s) 26, 586, 587, 640, 641
BmiI GGNNCC 3 cut(s) 28, 588, 642
BmrFI CCNGG 5 cut(s) 32, 302, 350, 401, 548
BmsI GCATC 3 cut(s) 444, 646, 885
Bpu10I CCTNAGC 1 cut(s) 6
BsaJI CCNNGG 4 cut(s) 30, 31, 710, 770
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 355, 457
BseBI CCWGG 5 cut(s) 32, 302, 350, 401, 548
BseDI CCNNGG 4 cut(s) 30, 31, 710, 770
BseGI GGATG 2 cut(s) 876, 906
BseLI CCNNNNNNNGG 3 cut(s) 70, 355, 457
BseSI GKGCMC 3 cut(s) 309, 590, 644
BseXI GCAGC 5 cut(s) 33, 61, 418, 421, 883
BsgI GTGCAG 3 cut(s) 275, 374, 425
BshFI GGCC 3 cut(s) 27, 588, 642
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 1 cut(s) 664
BslI CCNNNNNNNGG 3 cut(s) 70, 355, 457
BsnI GGCC 3 cut(s) 27, 588, 642
Bsp120I GGGCCC 2 cut(s) 586, 640
Bsp1286I GDGCHC 3 cut(s) 309, 590, 644
Bsp143I GATC 4 cut(s) 11, 514, 564, 859
BspACI CCGC 1 cut(s) 285
BspANI GGCC 3 cut(s) 27, 588, 642
BspHI TCATGA 1 cut(s) 87
BspLI GGNNCC 3 cut(s) 28, 588, 642
BspMAI CTGCAG 1 cut(s) 51
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 1 cut(s) 19
BspQI GCTCTTC 1 cut(s) 138
BssECI CCNNGG 4 cut(s) 30, 31, 710, 770
BssMI GATC 4 cut(s) 11, 514, 564, 859
BssSI CACGAG 1 cut(s) 68
BssT1I CCWWGG 2 cut(s) 710, 770
Bst2BI CACGAG 1 cut(s) 68
Bst2UI CCWGG 5 cut(s) 32, 302, 350, 401, 548
Bst6I CTCTTC 3 cut(s) 32, 138, 850
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 1 cut(s) 6
BstENI CCTNNNNNAGG 1 cut(s) 68
BstF5I GGATG 2 cut(s) 876, 906
BstHHI GCGC 1 cut(s) 876
BstKTI GATC 4 cut(s) 14, 517, 567, 862
BstMBI GATC 4 cut(s) 11, 514, 564, 859
BstMWI GCNNNNNNNGC 2 cut(s) 91, 440
BstNI CCWGG 5 cut(s) 32, 302, 350, 401, 548
BstSCI CCNGG 5 cut(s) 30, 300, 348, 399, 546
BstSFI CTRYAG 1 cut(s) 47
BstSLI GKGCMC 3 cut(s) 309, 590, 644
BstV1I GCAGC 5 cut(s) 33, 61, 418, 421, 883
BstX2I RGATCY 2 cut(s) 11, 859
BstYI RGATCY 2 cut(s) 11, 859
BsuRI GGCC 3 cut(s) 27, 588, 642
BtsCI GGATG 2 cut(s) 876, 906
BtsI GCAGTG 2 cut(s) 710, 778
BtsIMutI CAGTG 3 cut(s) 710, 745, 778
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 432
CciI TCATGA 1 cut(s) 87
CfoI GCGC 1 cut(s) 876
Cfr13I GGNCC 5 cut(s) 26, 586, 587, 640, 641
CseI GACGC 1 cut(s) 91
CsiI ACCWGGT 1 cut(s) 300
Csp6I GTAC 2 cut(s) 127, 314
CspCI CAANNNNNGTGG 2 cut(s) 408, 443
CviAII CATG 4 cut(s) 88, 130, 251, 512
CviQI GTAC 2 cut(s) 127, 314
DdeI CTNAG 1 cut(s) 6
DpnI GATC 4 cut(s) 13, 516, 566, 861
DpnII GATC 4 cut(s) 11, 514, 564, 859
DrdI GACNNNNNNGTC 1 cut(s) 542
DseDI GACNNNNNNGTC 1 cut(s) 542
Eam1104I CTCTTC 3 cut(s) 32, 138, 850
EarI CTCTTC 3 cut(s) 32, 138, 850
Eco130I CCWWGG 2 cut(s) 710, 770
Eco24I GRGCYC 2 cut(s) 590, 644
Eco57I CTGAAG 2 cut(s) 677, 851
EcoNI CCTNNNNNAGG 1 cut(s) 68
EcoO109I RGGNCCY 3 cut(s) 26, 587, 641
EcoRII CCWGG 5 cut(s) 30, 300, 348, 399, 546
EcoT14I CCWWGG 2 cut(s) 710, 770
EcoT38I GRGCYC 2 cut(s) 590, 644
ErhI CCWWGG 2 cut(s) 710, 770
FaeI CATG 4 cut(s) 91, 133, 254, 515
FaiI YATR 9 cut(s) 89, 131, 197, 252, 513, 519, 521, 557, 563
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 4 cut(s) 87, 129, 250, 511
FauI CCCGC 1 cut(s) 292
FauNDI CATATG 1 cut(s) 519
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 5 cut(s) 47, 50, 407, 435, 872
FokI GGATG 1 cut(s) 863
FriOI GRGCYC 2 cut(s) 590, 644
Fsp4HI GCNGC 5 cut(s) 47, 50, 407, 435, 872
FspBI CTAG 2 cut(s) 179, 476
GlaI GCGC 1 cut(s) 875
GluI GCNGC 5 cut(s) 47, 50, 407, 435, 872
HaeIII GGCC 3 cut(s) 27, 588, 642
HapII CCGG 1 cut(s) 664
HgaI GACGC 1 cut(s) 91
HhaI GCGC 1 cut(s) 876
Hin1II CATG 4 cut(s) 91, 133, 254, 515
Hin6I GCGC 1 cut(s) 874
HinP1I GCGC 1 cut(s) 874
HindIII AAGCTT 4 cut(s) 579, 626, 633, 689
HinfI GANTC 3 cut(s) 122, 396, 719
HpaII CCGG 1 cut(s) 664
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 1 cut(s) 696
Hpy188III TCNNGA 1 cut(s) 88
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 2 cut(s) 17, 891
HpyCH4IV ACGT 1 cut(s) 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 440
HpyF3I CTNAG 1 cut(s) 6
HpySE526I ACGT 1 cut(s) 883
Hsp92II CATG 4 cut(s) 91, 133, 254, 515
HspAI GCGC 1 cut(s) 874
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 4 cut(s) 11, 514, 564, 859
LguI GCTCTTC 1 cut(s) 138
Lsp1109I GCAGC 5 cut(s) 33, 61, 418, 421, 883
LweI GCATC 3 cut(s) 444, 646, 885
MabI ACCWGGT 1 cut(s) 300
MaeI CTAG 2 cut(s) 179, 476
MaeII ACGT 1 cut(s) 883
MalI GATC 4 cut(s) 13, 516, 566, 861
MboI GATC 4 cut(s) 11, 514, 564, 859
MboII GAAGA 8 cut(s) 6, 49, 155, 473, 476, 854, 857, 867
MfeI CAATTG 1 cut(s) 18
MflI RGATCY 2 cut(s) 11, 859
MhlI GDGCHC 3 cut(s) 309, 590, 644
MluCI AATT 9 cut(s) 18, 190, 274, 325, 373, 410, 698, 734, 787
MlyI GAGTC 1 cut(s) 405
MmeI TCCRAC 2 cut(s) 634, 904
MnlI CCTC 5 cut(s) 33, 64, 600, 654, 836
MroXI GAANNNNTTC 1 cut(s) 573
MseI TTAA 4 cut(s) 57, 278, 524, 618
MspI CCGG 1 cut(s) 664
MspR9I CCNGG 5 cut(s) 32, 302, 350, 401, 548
MunI CAATTG 1 cut(s) 18
MvaI CCWGG 5 cut(s) 32, 302, 350, 401, 548
MwoI GCNNNNNNNGC 2 cut(s) 91, 440
NdeI CATATG 1 cut(s) 519
NdeII GATC 4 cut(s) 11, 514, 564, 859
NlaIII CATG 4 cut(s) 91, 133, 254, 515
NlaIV GGNNCC 3 cut(s) 28, 588, 642
PagI TCATGA 1 cut(s) 87
PasI CCCWGGG 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 889
PdmI GAANNNNTTC 1 cut(s) 573
PfeI GAWTC 2 cut(s) 122, 719
PfoI TCCNGGA 1 cut(s) 546
PkrI GCNGC 5 cut(s) 48, 51, 408, 436, 873
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
Psp6I CCWGG 5 cut(s) 30, 300, 348, 399, 546
PspGI CCWGG 5 cut(s) 30, 300, 348, 399, 546
PspN4I GGNNCC 3 cut(s) 28, 588, 642
PspOMI GGGCCC 2 cut(s) 586, 640
PspPI GGNCC 5 cut(s) 26, 586, 587, 640, 641
PstI CTGCAG 1 cut(s) 51
PsuI RGATCY 2 cut(s) 11, 859
RsaI GTAC 2 cut(s) 128, 315
RsaNI GTAC 2 cut(s) 127, 314
SapI GCTCTTC 1 cut(s) 138
SaqAI TTAA 4 cut(s) 57, 278, 524, 618
SatI GCNGC 5 cut(s) 47, 50, 407, 435, 872
Sau3AI GATC 4 cut(s) 11, 514, 564, 859
Sau96I GGNCC 5 cut(s) 26, 586, 587, 640, 641
SchI GAGTC 1 cut(s) 405
ScrFI CCNGG 5 cut(s) 32, 302, 350, 401, 548
SduI GDGCHC 3 cut(s) 309, 590, 644
SexAI ACCWGGT 1 cut(s) 300
SfaNI GCATC 3 cut(s) 444, 646, 885
SfcI CTRYAG 1 cut(s) 47
Sse9I AATT 9 cut(s) 18, 190, 274, 325, 373, 410, 698, 734, 787
SsiI CCGC 1 cut(s) 285
SspMI CTAG 2 cut(s) 179, 476
StyD4I CCNGG 5 cut(s) 30, 300, 348, 399, 546
StyI CCWWGG 2 cut(s) 710, 770
TaiI ACGT 1 cut(s) 886
TaqI TCGA 1 cut(s) 567
TasI AATT 9 cut(s) 18, 190, 274, 325, 373, 410, 698, 734, 787
TatI WGTACW 1 cut(s) 313
TfiI GAWTC 2 cut(s) 122, 719
Tru1I TTAA 4 cut(s) 57, 278, 524, 618
Tru9I TTAA 4 cut(s) 57, 278, 524, 618
TscAI CASTG 3 cut(s) 710, 745, 785
TseI GCWGC 5 cut(s) 46, 49, 406, 434, 871
TspDTI ATGAA 3 cut(s) 104, 184, 615
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 3 cut(s) 710, 745, 785
VneI GTGCAC 1 cut(s) 305
XagI CCTNNNNNAGG 1 cut(s) 68
XapI RAATTY 5 cut(s) 190, 274, 325, 373, 734
XmnI GAANNNNTTC 1 cut(s) 573
XspI CTAG 2 cut(s) 179, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.