RLG00000023173

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008108
Physical Location & Seq
Reverse (-)
19458630 .. 19460925
2296 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023173

Sequence Viewer

Length: 1050 bp
ATGTATACGACTGCGTTTGATCGGAAATGCTGCATCCCTTGGCGCCTAAACACTGCCAGCCCCATGACTGATTTCACCCTTATTGATTCTGAGACCGAGATCGAAGCCTCTCGAGGTACCAGTAGAACAAGAGTGAATATGGCTGAGGGATCTTCAATTGAAGGTCCCCAGGGGAAGAGGTTGCTGCAGCGCCTTAAAGACCTACACGAGGAAAACAAGACACTCATGAAGCTCGTTGCAGACAAAGATAAGCGTATTGAATCGTACATGATAGATTTTGAAGAGCTGAATAGCTTGAAAATCATTGTCAATAAACTAGAGAAACAAAATTTCAAACTCACCCAAGCAAACAGCCAGATGTTGCAAGAGCTACGGAGTTTGAGAAACCATGTGCAGAAAGTACAACAACCAAATTTACCACCCGCCCAAGAAATAAACCAGGTGCAGGAAGTACAACAACCAAATTTGCAACCCCAAGAAATAATCCAGGTGCAGGAAGCACAACAACCAAATTTGCAACCTGCCCAAGCAAGAGTCCAGGTGCAGGAATTAGAACCACCAAATTTGCAAGCTGCCCAAGCAAGTAGCCAGATGCAGGAAGAAGAACCTGACCTAGAAAATAGCCGGATATTGCTGGAACTCAAATCACATGATCATATGTTGAGTTCTACTGACGAAGCAGTCCGGGAGTGTATAGATATGATCGAAAAACTTCAAGCTTTGGGCCCTCCTACTAATGAAGCAATCCAACAAGTTAAGGCAAAGCTTCAAGCTTTGGGCCCTCTTACGATAGATGCAGTCCGGCAGATTAGAGTGGGGTTTCGTAAGCTTCAGAAATTGCAGTGTCCAAGGTGTGAATCTAATAACACTAAATTCAGTGTTTTGAAAGCCAAAAGAATATCTGCCCCAAGGTATCACTGCAAAAATTGCAAAATGGAATGGACATTAGGGGGCGAAATCAGGAAACCTATTGTGCTTGGAGGCGTGAATAGAAGAGATCTTCTTCAGCAGCGCGTCCAACGTGTTCAGCGTAGAAGGATGAAGGCTTGA

Protein Analysis

350

Amino Acids

40.11

Weight (kDa)

9.19

Isoelectric Point (pI)

61.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 279 - 332 8.7e-17 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
Acc36I ACCTGC 1 cut(s) 529
Acc65I GGTACC 1 cut(s) 116
AccB1I GGYRCC 2 cut(s) 42, 116
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 1014
AciI CCGC 1 cut(s) 423
AclWI GGATC 1 cut(s) 157
AcsI RAATTY 6 cut(s) 328, 412, 463, 511, 562, 872
AcuI CTGAAG 2 cut(s) 815, 989
AcyI GRCGYC 1 cut(s) 43
AfaI GTAC 4 cut(s) 118, 266, 402, 453
AfiI CCNNNNNNNGG 5 cut(s) 208, 445, 493, 544, 595
AflIII ACRYGT 1 cut(s) 1021
AgsI TTSAA 9 cut(s) 156, 161, 260, 281, 298, 334, 716, 770, 886
AjnI CCWGG 4 cut(s) 168, 438, 486, 537
AluBI AGCT 9 cut(s) 232, 286, 294, 370, 572, 719, 766, 773, 829
AluI AGCT 9 cut(s) 232, 286, 294, 370, 572, 719, 766, 773, 829
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 157
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 2 cut(s) 724, 778
ApaI GGGCCC 2 cut(s) 728, 782
ApeKI GCWGC 5 cut(s) 30, 184, 187, 572, 1009
ApoI RAATTY 6 cut(s) 328, 412, 463, 511, 562, 872
Asp700I GAANNNNTTC 1 cut(s) 711
Asp718I GGTACC 1 cut(s) 116
AspLEI GCGC 3 cut(s) 45, 192, 1014
AspS9I GGNCC 5 cut(s) 164, 724, 725, 778, 779
AsuC2I CCSGG 1 cut(s) 686
AsuHPI GGTGA 2 cut(s) 67, 331
AvaI CYCGRG 1 cut(s) 111
AvaII GGWCC 1 cut(s) 164
BaeGI GKGCMC 2 cut(s) 728, 782
BanI GGYRCC 2 cut(s) 42, 116
BanII GRGCYC 2 cut(s) 728, 782
BauI CACGAG 1 cut(s) 206
BbvCI CCTCAGC 1 cut(s) 144
BbvI GCAGC 5 cut(s) 17, 171, 199, 559, 1021
BciT130I CCWGG 4 cut(s) 170, 440, 488, 539
BclI TGATCA 1 cut(s) 652
BcnI CCSGG 1 cut(s) 686
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 2 cut(s) 317, 614
BfmI CTRYAG 1 cut(s) 185
BfoI RGCGCY 2 cut(s) 46, 193
BfuAI ACCTGC 1 cut(s) 529
BglII AGATCT 1 cut(s) 997
BisI GCNGC 5 cut(s) 31, 185, 188, 573, 1010
BlsI GCNGC 5 cut(s) 32, 186, 189, 574, 1011
Bme1390I CCNGG 5 cut(s) 170, 440, 488, 539, 686
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 111
BmgT120I GGNCC 5 cut(s) 164, 724, 725, 778, 779
BmiI GGNNCC 5 cut(s) 44, 118, 166, 726, 780
BmrFI CCNGG 5 cut(s) 170, 440, 488, 539, 686
BmsI GCATC 3 cut(s) 42, 582, 784
Bpu10I CCTNAGC 1 cut(s) 144
BpuMI CCSGG 1 cut(s) 686
BsaHI GRCGYC 1 cut(s) 43
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 5 cut(s) 38, 168, 169, 848, 908
Bsc4I CCNNNNNNNGG 5 cut(s) 208, 445, 493, 544, 595
Bse1I ACTGG 1 cut(s) 120
BseBI CCWGG 4 cut(s) 170, 440, 488, 539
BseDI CCNNGG 5 cut(s) 38, 168, 169, 848, 908
BseGI GGATG 2 cut(s) 33, 1044
BseLI CCNNNNNNNGG 5 cut(s) 208, 445, 493, 544, 595
BseMII CTCAG 2 cut(s) 81, 135
BseNI ACTGG 1 cut(s) 120
BseSI GKGCMC 2 cut(s) 728, 782
BseXI GCAGC 5 cut(s) 17, 171, 199, 559, 1021
BsgI GTGCAG 4 cut(s) 413, 464, 512, 563
Bsh1236I CGCG 1 cut(s) 1014
BshFI GGCC 2 cut(s) 726, 780
BshNI GGYRCC 2 cut(s) 42, 116
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 3 cut(s) 625, 685, 802
BslFI GGGAC 1 cut(s) 150
BslI CCNNNNNNNGG 5 cut(s) 208, 445, 493, 544, 595
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 150
BsnI GGCC 2 cut(s) 726, 780
Bso31I GGTCTC 1 cut(s) 86
BsoBI CYCGRG 1 cut(s) 111
Bsp120I GGGCCC 2 cut(s) 724, 778
Bsp1286I GDGCHC 2 cut(s) 728, 782
Bsp143I GATC 6 cut(s) 19, 99, 149, 652, 702, 997
BspACI CCGC 1 cut(s) 423
BspANI GGCC 2 cut(s) 726, 780
BspCNI CTCAG 2 cut(s) 82, 136
BspFNI CGCG 1 cut(s) 1014
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 5 cut(s) 44, 118, 166, 726, 780
BspMAI CTGCAG 1 cut(s) 189
BspMI ACCTGC 1 cut(s) 529
BspPI GGATC 1 cut(s) 157
BspQI GCTCTTC 1 cut(s) 276
BspT107I GGYRCC 2 cut(s) 42, 116
BspTNI GGTCTC 1 cut(s) 86
BsrI ACTGG 1 cut(s) 120
BssECI CCNNGG 5 cut(s) 38, 168, 169, 848, 908
BssMI GATC 6 cut(s) 19, 99, 149, 652, 702, 997
BssNAI GTATAC 1 cut(s) 6
BssNI GRCGYC 1 cut(s) 43
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 3 cut(s) 38, 848, 908
Bst1107I GTATAC 1 cut(s) 6
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 4 cut(s) 170, 440, 488, 539
Bst6I CTCTTC 3 cut(s) 170, 276, 988
BstACI GRCGYC 1 cut(s) 43
BstAPI GCANNNNNTGC 1 cut(s) 927
BstC8I GCNNGC 2 cut(s) 58, 570
BstDEI CTNAG 2 cut(s) 90, 144
BstENI CCTNNNNNAGG 1 cut(s) 206
BstF5I GGATG 2 cut(s) 33, 1044
BstFNI CGCG 1 cut(s) 1014
BstH2I RGCGCY 2 cut(s) 46, 193
BstHHI GCGC 3 cut(s) 45, 192, 1014
BstKTI GATC 6 cut(s) 22, 102, 152, 655, 705, 1000
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 6 cut(s) 19, 99, 149, 652, 702, 997
BstMWI GCNNNNNNNGC 2 cut(s) 578, 927
BstNI CCWGG 4 cut(s) 170, 440, 488, 539
BstSCI CCNGG 5 cut(s) 168, 438, 486, 537, 684
BstSFI CTRYAG 1 cut(s) 185
BstSLI GKGCMC 2 cut(s) 728, 782
BstUI CGCG 1 cut(s) 1014
BstV1I GCAGC 5 cut(s) 17, 171, 199, 559, 1021
BstX2I RGATCY 2 cut(s) 149, 997
BstYI RGATCY 2 cut(s) 149, 997
BstZ17I GTATAC 1 cut(s) 6
BsuRI GGCC 2 cut(s) 726, 780
BtsCI GGATG 2 cut(s) 33, 1044
BtsI GCAGTG 3 cut(s) 51, 848, 916
BtsIMutI CAGTG 4 cut(s) 51, 848, 883, 916
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 2 cut(s) 58, 570
CciI TCATGA 1 cut(s) 225
CfoI GCGC 3 cut(s) 45, 192, 1014
Cfr13I GGNCC 5 cut(s) 164, 724, 725, 778, 779
CseI GACGC 1 cut(s) 1003
CsiI ACCWGGT 1 cut(s) 438
Csp6I GTAC 4 cut(s) 117, 265, 401, 452
CspCI CAANNNNNGTGG 2 cut(s) 546, 581
CviAII CATG 5 cut(s) 64, 226, 268, 389, 650
CviQI GTAC 4 cut(s) 117, 265, 401, 452
DdeI CTNAG 2 cut(s) 90, 144
DinI GGCGCC 1 cut(s) 44
DpnI GATC 6 cut(s) 21, 101, 151, 654, 704, 999
DpnII GATC 6 cut(s) 19, 99, 149, 652, 702, 997
DrdI GACNNNNNNGTC 1 cut(s) 680
DseDI GACNNNNNNGTC 1 cut(s) 680
Eam1104I CTCTTC 3 cut(s) 170, 276, 988
EarI CTCTTC 3 cut(s) 170, 276, 988
Eco130I CCWWGG 3 cut(s) 38, 848, 908
Eco24I GRGCYC 2 cut(s) 728, 782
Eco31I GGTCTC 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 164
Eco57I CTGAAG 2 cut(s) 815, 989
Eco88I CYCGRG 1 cut(s) 111
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoO109I RGGNCCY 3 cut(s) 164, 725, 779
EcoRII CCWGG 4 cut(s) 168, 438, 486, 537
EcoT14I CCWWGG 3 cut(s) 38, 848, 908
EcoT38I GRGCYC 2 cut(s) 728, 782
EgeI GGCGCC 1 cut(s) 44
EheI GGCGCC 1 cut(s) 44
ErhI CCWWGG 3 cut(s) 38, 848, 908
FaeI CATG 5 cut(s) 67, 229, 271, 392, 653
FaqI GGGAC 1 cut(s) 150
FatI CATG 5 cut(s) 63, 225, 267, 388, 649
FauI CCCGC 1 cut(s) 430
FauNDI CATATG 1 cut(s) 657
FbaI TGATCA 1 cut(s) 652
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 5 cut(s) 31, 185, 188, 573, 1010
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 2 cut(s) 728, 782
Fsp4HI GCNGC 5 cut(s) 31, 185, 188, 573, 1010
FspBI CTAG 2 cut(s) 317, 614
GlaI GCGC 3 cut(s) 44, 191, 1013
GluI GCNGC 5 cut(s) 31, 185, 188, 573, 1010
HaeII RGCGCY 2 cut(s) 46, 193
HaeIII GGCC 2 cut(s) 726, 780
HapII CCGG 3 cut(s) 625, 685, 802
HgaI GACGC 1 cut(s) 1003
HhaI GCGC 3 cut(s) 45, 192, 1014
Hin1I GRCGYC 1 cut(s) 43
Hin1II CATG 5 cut(s) 67, 229, 271, 392, 653
Hin6I GCGC 3 cut(s) 43, 190, 1012
HinP1I GCGC 3 cut(s) 43, 190, 1012
HindIII AAGCTT 4 cut(s) 717, 764, 771, 827
HinfI GANTC 4 cut(s) 86, 260, 534, 857
HpaII CCGG 3 cut(s) 625, 685, 802
HphI GGTGA 2 cut(s) 67, 331
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 24, 91, 834
Hpy188III TCNNGA 3 cut(s) 111, 226, 961
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 3 cut(s) 155, 1029, 1036
HpyCH4IV ACGT 1 cut(s) 1021
HpyF10VI GCNNNNNNNGC 2 cut(s) 578, 927
HpyF3I CTNAG 2 cut(s) 90, 144
HpySE526I ACGT 1 cut(s) 1021
Hsp92I GRCGYC 1 cut(s) 43
Hsp92II CATG 5 cut(s) 67, 229, 271, 392, 653
HspAI GCGC 3 cut(s) 43, 190, 1012
KasI GGCGCC 1 cut(s) 42
KpnI GGTACC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 6 cut(s) 19, 99, 149, 652, 702, 997
LguI GCTCTTC 1 cut(s) 276
Lsp1109I GCAGC 5 cut(s) 17, 171, 199, 559, 1021
LweI GCATC 3 cut(s) 42, 582, 784
MabI ACCWGGT 1 cut(s) 438
MaeI CTAG 2 cut(s) 317, 614
MaeII ACGT 1 cut(s) 1021
MalI GATC 6 cut(s) 21, 101, 151, 654, 704, 999
MboI GATC 6 cut(s) 19, 99, 149, 652, 702, 997
MboII GAAGA 8 cut(s) 144, 187, 293, 611, 614, 992, 995, 1005
MfeI CAATTG 1 cut(s) 156
MflI RGATCY 2 cut(s) 149, 997
MhlI GDGCHC 2 cut(s) 728, 782
Mly113I GGCGCC 1 cut(s) 43
MlyI GAGTC 1 cut(s) 543
MmeI TCCRAC 2 cut(s) 772, 1042
MnlI CCTC 8 cut(s) 107, 118, 139, 171, 202, 738, 792, 974
MroXI GAANNNNTTC 1 cut(s) 711
MseI TTAA 2 cut(s) 195, 756
MspI CCGG 3 cut(s) 625, 685, 802
MspR9I CCNGG 5 cut(s) 170, 440, 488, 539, 686
MunI CAATTG 1 cut(s) 156
MvaI CCWGG 4 cut(s) 170, 440, 488, 539
MvnI CGCG 1 cut(s) 1014
MwoI GCNNNNNNNGC 2 cut(s) 578, 927
NarI GGCGCC 1 cut(s) 43
NciI CCSGG 1 cut(s) 686
NdeI CATATG 1 cut(s) 657
NdeII GATC 6 cut(s) 19, 99, 149, 652, 702, 997
NlaIII CATG 5 cut(s) 67, 229, 271, 392, 653
NlaIV GGNNCC 5 cut(s) 44, 118, 166, 726, 780
PaeR7I CTCGAG 1 cut(s) 111
PagI TCATGA 1 cut(s) 225
PasI CCCWGGG 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 1027
PdmI GAANNNNTTC 1 cut(s) 711
PfeI GAWTC 3 cut(s) 86, 260, 857
PfoI TCCNGGA 1 cut(s) 684
PkrI GCNGC 5 cut(s) 32, 186, 189, 574, 1011
PleI GAGTC 1 cut(s) 542
PluTI GGCGCC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 542
PpuMI RGGWCCY 1 cut(s) 164
Psp5II RGGWCCY 1 cut(s) 164
Psp6I CCWGG 4 cut(s) 168, 438, 486, 537
PspGI CCWGG 4 cut(s) 168, 438, 486, 537
PspN4I GGNNCC 5 cut(s) 44, 118, 166, 726, 780
PspOMI GGGCCC 2 cut(s) 724, 778
PspPI GGNCC 5 cut(s) 164, 724, 725, 778, 779
PspPPI RGGWCCY 1 cut(s) 164
PstI CTGCAG 1 cut(s) 189
PsuI RGATCY 2 cut(s) 149, 997
RsaI GTAC 4 cut(s) 118, 266, 402, 453
RsaNI GTAC 4 cut(s) 117, 265, 401, 452
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 2 cut(s) 195, 756
SatI GCNGC 5 cut(s) 31, 185, 188, 573, 1010
Sau3AI GATC 6 cut(s) 19, 99, 149, 652, 702, 997
Sau96I GGNCC 5 cut(s) 164, 724, 725, 778, 779
SchI GAGTC 1 cut(s) 543
ScrFI CCNGG 5 cut(s) 170, 440, 488, 539, 686
SduI GDGCHC 2 cut(s) 728, 782
SexAI ACCWGGT 1 cut(s) 438
SfaNI GCATC 3 cut(s) 42, 582, 784
SfcI CTRYAG 1 cut(s) 185
SfoI GGCGCC 1 cut(s) 44
Sfr274I CTCGAG 1 cut(s) 111
SinI GGWCC 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 111
SmoI CTYRAG 1 cut(s) 111
SsiI CCGC 1 cut(s) 423
SspDI GGCGCC 1 cut(s) 42
SspMI CTAG 2 cut(s) 317, 614
StyD4I CCNGG 5 cut(s) 168, 438, 486, 537, 684
StyI CCWWGG 3 cut(s) 38, 848, 908
TaiI ACGT 1 cut(s) 1024
TaqI TCGA 3 cut(s) 102, 112, 705
TaqII GACCGA 1 cut(s) 110
TatI WGTACW 2 cut(s) 400, 451
TfiI GAWTC 3 cut(s) 86, 260, 857
Tru1I TTAA 2 cut(s) 195, 756
Tru9I TTAA 2 cut(s) 195, 756
TscAI CASTG 4 cut(s) 58, 848, 883, 923
TseI GCWGC 5 cut(s) 30, 184, 187, 572, 1009
TspDTI ATGAA 2 cut(s) 242, 753
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 4 cut(s) 58, 848, 883, 923
VpaK11BI GGWCC 1 cut(s) 164
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 6 cut(s) 328, 412, 463, 511, 562, 872
XhoI CTCGAG 1 cut(s) 111
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 711
XspI CTAG 2 cut(s) 317, 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.