RchiOBHm_Chr4g0387711

Catalyzes the formation of formate and 2-keto-4- methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5- methylthiopentene (DHK-MTPene)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002757
Physical Location & Seq
Forward (+)
2923071 .. 2925547
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36100

Sequence Viewer

Length: 726 bp
ATGTTGGATTTGATTGTTATTATGATTGCTGAAGCAGACCCGAGAGAGGAAGTCATTCAAGCATGGTACATGGATGATAGTGATGAGGACCAGAGACTACCCCATCACCGCGAGCCCAAGGAATTTCTTGGGGTTCTCAGCTGGCGTTTAGATGCTGATAACTATGAAACCGATGAGGAGTTGAAGAAGATTCGTGATGACCGTGGTTACTCCTACATGGACTTCTGTGAGGTTTGCCCTGAAAAGCTTCCAAATTATGAGGAGAAGATAAAGAACTTCTTTGAGGAACATCTTCACACTGATGAGGAGATCCGCTATGCTGTGGCAGGAAGTGGTGACTTTTTACTTTCTTGTGAAGGTTATTTTGATGTTAGGGACTGTAACGAACGCTGGATTCGTGTATGGCTGAAAAAGGGTGGATTGATTGTTTTGCCTGCTGGAATTTATCACCGCTTTACTTTGGACACAGACAACTACATAAAGGCAATGCGGCTTTTCAGTGGTGATCCAATTTGGACTCCATTCAATCGCCCTCATGACGATCTTCCAGCAAGGAAGGAGTACCTCAAAGCTTTTGTGGATAAAGAAGCTGGTGAACATGCATTGATGAGGTTGTTTGTGGGAGAGCCTGTATGGACTGCTTACAATCGACCACAGGAAGAACATCCAGCTAGGAAGGAGTACATTGAGAGAAGGGCTTCTTCTACTGTTCTTACAAAGTCTTAG

Protein Analysis

241

Amino Acids

28.56

Weight (kDa)

4.97

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARD PF03079 20 - 174 8.5e-53 ARD/ARD' family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 111
AciI CCGC 4 cut(s) 109, 313, 451, 490
AclWI GGATC 2 cut(s) 304, 500
AcsI RAATTY 2 cut(s) 122, 441
AcuI CTGAAG 1 cut(s) 51
AfaI GTAC 3 cut(s) 68, 563, 683
AfiI CCNNNNNNNGG 1 cut(s) 46
AgsI TTSAA 3 cut(s) 59, 184, 526
AluBI AGCT 5 cut(s) 141, 247, 572, 590, 671
AluI AGCT 5 cut(s) 141, 247, 572, 590, 671
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 2 cut(s) 304, 500
Ama87I CYCGRG 1 cut(s) 40
ApoI RAATTY 2 cut(s) 122, 441
Asp700I GAANNNNTTC 4 cut(s) 54, 246, 291, 697
AspS9I GGNCC 1 cut(s) 88
AsuHPI GGTGA 5 cut(s) 98, 347, 440, 515, 605
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 1 cut(s) 88
BanII GRGCYC 1 cut(s) 117
BccI CCATC 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 1 cut(s) 672
BisI GCNGC 1 cut(s) 491
BlsI GCNGC 1 cut(s) 492
Bme18I GGWCC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 40
BmgT120I GGNCC 1 cut(s) 88
BmsI GCATC 1 cut(s) 142
BsaJI CCNNGG 2 cut(s) 117, 202
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse3DI GCAATG 1 cut(s) 492
BseDI CCNNGG 2 cut(s) 117, 202
BseGI GGATG 2 cut(s) 79, 664
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 1 cut(s) 151
BseRI GAGGAG 3 cut(s) 191, 275, 320
Bsh1236I CGCG 1 cut(s) 111
BsiHKCI CYCGRG 1 cut(s) 40
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 389
BsoBI CYCGRG 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 3 cut(s) 309, 505, 541
BspACI CCGC 4 cut(s) 109, 313, 451, 490
BspCNI CTCAG 1 cut(s) 150
BspFNI CGCG 1 cut(s) 111
BspHI TCATGA 1 cut(s) 535
BspPI GGATC 2 cut(s) 304, 500
BsrDI GCAATG 1 cut(s) 492
BssECI CCNNGG 2 cut(s) 117, 202
BssMI GATC 3 cut(s) 309, 505, 541
BssT1I CCWWGG 1 cut(s) 117
Bst4CI ACNGT 3 cut(s) 203, 380, 709
BstC8I GCNNGC 3 cut(s) 113, 143, 435
BstDEI CTNAG 2 cut(s) 137, 723
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 2 cut(s) 79, 664
BstFNI CGCG 1 cut(s) 111
BstKTI GATC 3 cut(s) 312, 508, 544
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 3 cut(s) 309, 505, 541
BstNSI RCATGY 1 cut(s) 602
BstUI CGCG 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 309
BstYI RGATCY 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 2 cut(s) 79, 664
BtsIMutI CAGTG 2 cut(s) 297, 505
Cac8I GCNNGC 3 cut(s) 113, 143, 435
CciI TCATGA 1 cut(s) 535
Cfr13I GGNCC 1 cut(s) 88
Csp6I GTAC 3 cut(s) 67, 562, 682
CviAII CATG 5 cut(s) 63, 70, 217, 536, 599
CviQI GTAC 3 cut(s) 67, 562, 682
DdeI CTNAG 2 cut(s) 137, 723
DpnI GATC 3 cut(s) 311, 507, 543
DpnII GATC 3 cut(s) 309, 505, 541
Eco130I CCWWGG 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 117
Eco47I GGWCC 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 604
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 5 cut(s) 66, 73, 220, 539, 602
FalI AAGNNNNNCTT 4 cut(s) 263, 295, 685, 717
FaqI GGGAC 1 cut(s) 389
FatI CATG 5 cut(s) 62, 69, 216, 535, 598
Fnu4HI GCNGC 1 cut(s) 491
FokI GGATG 2 cut(s) 86, 651
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 1 cut(s) 491
FspBI CTAG 1 cut(s) 672
GluI GCNGC 1 cut(s) 491
Hin1II CATG 5 cut(s) 66, 73, 220, 539, 602
HindIII AAGCTT 2 cut(s) 245, 570
HinfI GANTC 3 cut(s) 190, 394, 517
HphI GGTGA 5 cut(s) 98, 347, 440, 515, 605
Hpy166II GTNNAC 1 cut(s) 596
Hpy188III TCNNGA 2 cut(s) 194, 536
Hpy8I GTNNAC 1 cut(s) 596
HpyAV CCTTC 4 cut(s) 350, 550, 670, 687
HpyCH4III ACNGT 3 cut(s) 203, 380, 709
HpyCH4V TGCA 1 cut(s) 602
HpyF3I CTNAG 2 cut(s) 137, 723
Hsp92II CATG 5 cut(s) 66, 73, 220, 539, 602
Kzo9I GATC 3 cut(s) 309, 505, 541
LweI GCATC 1 cut(s) 142
MaeI CTAG 1 cut(s) 672
MaeIII GTNAC 3 cut(s) 206, 335, 380
MalI GATC 3 cut(s) 311, 507, 543
MboI GATC 3 cut(s) 309, 505, 541
MboII GAAGA 7 cut(s) 196, 199, 277, 284, 536, 671, 693
MflI RGATCY 1 cut(s) 309
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 4 cut(s) 122, 253, 441, 510
MlyI GAGTC 1 cut(s) 511
Mph1103I ATGCAT 1 cut(s) 604
MroXI GAANNNNTTC 4 cut(s) 54, 246, 291, 697
MslI CAYNNNNRTG 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 141
MvnI CGCG 1 cut(s) 111
NdeII GATC 3 cut(s) 309, 505, 541
NlaIII CATG 5 cut(s) 66, 73, 220, 539, 602
NmuCI GTSAC 1 cut(s) 335
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 1 cut(s) 602
PagI TCATGA 1 cut(s) 535
PcsI WCGNNNNNNNCGW 2 cut(s) 199, 394
PdmI GAANNNNTTC 4 cut(s) 54, 246, 291, 697
PfeI GAWTC 2 cut(s) 190, 394
PkrI GCNGC 1 cut(s) 492
PleI GAGTC 1 cut(s) 511
PpsI GAGTC 1 cut(s) 511
PspPI GGNCC 1 cut(s) 88
PsuI RGATCY 1 cut(s) 309
PvuII CAGCTG 1 cut(s) 141
RsaI GTAC 3 cut(s) 68, 563, 683
RsaNI GTAC 3 cut(s) 67, 562, 682
RseI CAYNNNNRTG 1 cut(s) 300
SatI GCNGC 1 cut(s) 491
Sau3AI GATC 3 cut(s) 309, 505, 541
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 1 cut(s) 511
SduI GDGCHC 1 cut(s) 117
SetI ASST 9 cut(s) 143, 234, 249, 361, 567, 574, 592, 614, 673
SfaNI GCATC 1 cut(s) 142
SinI GGWCC 1 cut(s) 88
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 4 cut(s) 122, 253, 441, 510
SsiI CCGC 4 cut(s) 109, 313, 451, 490
SspMI CTAG 1 cut(s) 672
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 3 cut(s) 203, 380, 709
TaqI TCGA 1 cut(s) 649
TasI AATT 4 cut(s) 122, 253, 441, 510
TatI WGTACW 1 cut(s) 681
TauI GCSGC 1 cut(s) 493
TfiI GAWTC 2 cut(s) 190, 394
TscAI CASTG 2 cut(s) 304, 505
TseFI GTSAC 1 cut(s) 335
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 180
TspRI CASTG 2 cut(s) 304, 505
VpaK11BI GGWCC 1 cut(s) 88
XapI RAATTY 2 cut(s) 122, 441
XceI RCATGY 1 cut(s) 602
XmnI GAANNNNTTC 4 cut(s) 54, 246, 291, 697
XspI CTAG 1 cut(s) 672
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.