Rorug03G0312900

Catalyzes the formation of formate and 2-keto-4- methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5- methylthiopentene (DHK-MTPene)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002757
Physical Location & Seq
Reverse (-)
33513950 .. 33517816
3867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0312900.1

Sequence Viewer

Length: 855 bp
ATGGAGAATCCACGCCTCGTCTCTCTGAGGGCGCTTTTGGCTAGTTCTACAGCGGCTATCCACTCTACCGATTTTCCTACCTACCGGATCGGAACGGTTGGCGCTACTCGAACTGGGAAGTCCTTGTTTCTGAAAAAGGTAGTGGGCGAGTCAACCATTGATGGTTACGAAGCCACTGAGGTTCGAGAATCTTATACTTTGTATGGGTGCTGCAAAGGTGACAAAATCTTAGCCAGGCTTGTCTGCTTCGACGTTCCGGGTCGTTTTGGGGTTGACTTTGGTGGCTTTGAGGGCATTGAGGGCTTTGATGCTAATTCCCCACTCGGTGATGTGAAGGCTTTTCTCGTCTTCCATGATGTTACTAATTCTTCGTCATACACGGATGCCCAACGCTGGTGCAATCTCATCACCAGTGTATCTGGCCCTGGAGTCCCTGTTGCTATTTGTGGAACCAAGGTGGATCTGAGAAACAATGAAACAATGGTCGTCGGCCTTGATAACCAAATGAAGCACTTTGAGATTTCTGTGAAAACTGATTCCATGATCAAGCTCCAGGAACCACTGCTGTACCTTCTTACAGAGCTTATGGGCGCTACTGAGGTGGAGTATTTGAAAGCGCATGCTGGTTCTGAGCCTCCTGCAAAAAGTTCACTTGAAATTGATTTTGAAGCACAGAATGGGCCAGCCGCAGCAGCAGTGATTGCTGAGCCAGTTGCACGTGATGATGATGGATGGTTTGGCACTACCCTGAAGAAAATTAAAGAATGTTGCATGTGCTGTGCTCCACTCGTTCTTATCGCTCTGAAGATATGGGACTTTTTCTTCGGAACTGGGACCGGCTTGAATGAAAACTAA

Protein Analysis

284

Amino Acids

30.73

Weight (kDa)

4.96

Isoelectric Point (pI)

25.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 106 - 182 2.1e-08 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 53, 687
AclWI GGATC 2 cut(s) 95, 468
AcuI CTGAAG 2 cut(s) 770, 824
AcvI CACGTG 1 cut(s) 719
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 1 cut(s) 569
AfiI CCNNNNNNNGG 1 cut(s) 393
AgsI TTSAA 4 cut(s) 613, 656, 668, 844
AjnI CCWGG 3 cut(s) 233, 424, 552
AluBI AGCT 2 cut(s) 550, 583
AluI AGCT 2 cut(s) 550, 583
Alw21I GWGCWC 1 cut(s) 784
Alw26I GTCTC 1 cut(s) 25
AlwI GGATC 2 cut(s) 95, 468
AoxI GGCC 3 cut(s) 421, 490, 680
ApeKI GCWGC 3 cut(s) 210, 689, 692
ArsI GACNNNNNNTTYG 2 cut(s) 806, 838
AspLEI GCGC 4 cut(s) 34, 104, 593, 619
AspS9I GGNCC 3 cut(s) 422, 680, 834
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 3 cut(s) 230, 338, 400
AvaII GGWCC 1 cut(s) 834
BaeI ACNNNNGTAYC 4 cut(s) 399, 432, 551, 584
BbrPI CACGTG 1 cut(s) 719
BbsI GAAGAC 1 cut(s) 340
Bbv12I GWGCWC 1 cut(s) 784
BbvI GCAGC 3 cut(s) 197, 701, 704
BccI CCATC 3 cut(s) 155, 722, 726
BciT130I CCWGG 3 cut(s) 235, 426, 554
BclI TGATCA 1 cut(s) 543
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 1 cut(s) 42
BfmI CTRYAG 1 cut(s) 48
BfoI RGCGCY 3 cut(s) 35, 105, 594
BisI GCNGC 5 cut(s) 54, 211, 687, 690, 693
BlpI GCTNAGC 1 cut(s) 705
BlsI GCNGC 5 cut(s) 55, 212, 688, 691, 694
Bme1390I CCNGG 4 cut(s) 235, 258, 426, 554
Bme18I GGWCC 1 cut(s) 834
BmgT120I GGNCC 3 cut(s) 422, 680, 834
BmiI GGNNCC 3 cut(s) 451, 558, 835
BmrFI CCNGG 4 cut(s) 235, 258, 426, 554
BmrI ACTGGG 2 cut(s) 123, 840
BmsI GCATC 2 cut(s) 298, 373
BmuI ACTGGG 2 cut(s) 123, 840
BpiI GAAGAC 1 cut(s) 340
BpmI CTGGAG 2 cut(s) 447, 536
Bpu1102I GCTNAGC 1 cut(s) 705
BpuMI CCSGG 1 cut(s) 258
BsaAI YACGTR 1 cut(s) 719
BsaJI CCNNGG 2 cut(s) 424, 453
BsaWI WCCGGW 1 cut(s) 84
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 393
Bse118I RCCGGY 1 cut(s) 836
Bse1I ACTGG 4 cut(s) 118, 411, 710, 835
BseBI CCWGG 3 cut(s) 235, 426, 554
BseDI CCNNGG 2 cut(s) 424, 453
BseGI GGATG 2 cut(s) 388, 737
BseLI CCNNNNNNNGG 1 cut(s) 393
BseMII CTCAG 6 cut(s) 17, 168, 455, 588, 621, 696
BseNI ACTGG 4 cut(s) 118, 411, 710, 835
BseXI GCAGC 3 cut(s) 197, 701, 704
BshFI GGCC 3 cut(s) 423, 492, 682
BsiHKAI GWGCWC 1 cut(s) 784
BsiSI CCGG 3 cut(s) 85, 257, 837
BslFI GGGAC 3 cut(s) 416, 827, 847
BslI CCNNNNNNNGG 1 cut(s) 393
BsmAI GTCTC 1 cut(s) 25
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 3 cut(s) 416, 827, 847
BsnI GGCC 3 cut(s) 423, 492, 682
Bsp1286I GDGCHC 1 cut(s) 784
Bsp143I GATC 3 cut(s) 87, 460, 543
Bsp1720I GCTNAGC 1 cut(s) 705
BspACI CCGC 2 cut(s) 53, 687
BspANI GGCC 3 cut(s) 423, 492, 682
BspCNI CTCAG 6 cut(s) 18, 169, 456, 589, 622, 697
BspLI GGNNCC 3 cut(s) 451, 558, 835
BspPI GGATC 2 cut(s) 95, 468
BsrFI RCCGGY 1 cut(s) 836
BsrI ACTGG 4 cut(s) 118, 411, 710, 835
BssAI RCCGGY 1 cut(s) 836
BssECI CCNNGG 2 cut(s) 424, 453
BssMI GATC 3 cut(s) 87, 460, 543
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 3 cut(s) 235, 426, 554
Bst4CI ACNGT 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 701
BstBAI YACGTR 1 cut(s) 719
BstC8I GCNNGC 2 cut(s) 621, 684
BstDEI CTNAG 7 cut(s) 26, 177, 229, 464, 597, 630, 705
BstF5I GGATG 2 cut(s) 388, 737
BstH2I RGCGCY 3 cut(s) 35, 105, 594
BstHHI GCGC 4 cut(s) 34, 104, 593, 619
BstKTI GATC 3 cut(s) 90, 463, 546
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 3 cut(s) 87, 460, 543
BstMWI GCNNNNNNNGC 5 cut(s) 38, 291, 300, 692, 701
BstNI CCWGG 3 cut(s) 235, 426, 554
BstNSI RCATGY 2 cut(s) 623, 775
BstSCI CCNGG 4 cut(s) 233, 256, 424, 552
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 3 cut(s) 197, 701, 704
BstV2I GAAGAC 1 cut(s) 340
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BsuRI GGCC 3 cut(s) 423, 492, 682
BtsCI GGATG 2 cut(s) 388, 737
BtsI GCAGTG 2 cut(s) 560, 702
BtsIMutI CAGTG 4 cut(s) 174, 418, 560, 702
Cac8I GCNNGC 2 cut(s) 621, 684
CfoI GCGC 4 cut(s) 34, 104, 593, 619
Cfr10I RCCGGY 1 cut(s) 836
Cfr13I GGNCC 3 cut(s) 422, 680, 834
Csp6I GTAC 1 cut(s) 568
CviAII CATG 4 cut(s) 353, 541, 620, 772
CviQI GTAC 1 cut(s) 568
DdeI CTNAG 7 cut(s) 26, 177, 229, 464, 597, 630, 705
DpnI GATC 3 cut(s) 89, 462, 545
DpnII GATC 3 cut(s) 87, 460, 543
DraIII CACNNNGTG 1 cut(s) 326
Eco130I CCWWGG 1 cut(s) 453
Eco47I GGWCC 1 cut(s) 834
Eco57I CTGAAG 2 cut(s) 770, 824
Eco72I CACGTG 1 cut(s) 719
EcoRII CCWGG 3 cut(s) 233, 424, 552
EcoT14I CCWWGG 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 4 cut(s) 356, 544, 623, 775
FaiI YATR 9 cut(s) 195, 204, 354, 376, 542, 587, 621, 773, 811
FaqI GGGAC 3 cut(s) 416, 827, 847
FatI CATG 4 cut(s) 352, 540, 619, 771
FbaI TGATCA 1 cut(s) 543
Fnu4HI GCNGC 5 cut(s) 54, 211, 687, 690, 693
FokI GGATG 2 cut(s) 395, 744
Fsp4HI GCNGC 5 cut(s) 54, 211, 687, 690, 693
FspBI CTAG 1 cut(s) 42
GlaI GCGC 4 cut(s) 33, 103, 592, 618
GluI GCNGC 5 cut(s) 54, 211, 687, 690, 693
GsuI CTGGAG 2 cut(s) 447, 536
HaeII RGCGCY 3 cut(s) 35, 105, 594
HaeIII GGCC 3 cut(s) 423, 492, 682
HapII CCGG 3 cut(s) 85, 257, 837
HhaI GCGC 4 cut(s) 34, 104, 593, 619
Hin1II CATG 4 cut(s) 356, 544, 623, 775
Hin6I GCGC 4 cut(s) 32, 102, 591, 617
HinP1I GCGC 4 cut(s) 32, 102, 591, 617
HincII GTYRAC 2 cut(s) 153, 274
HindII GTYRAC 2 cut(s) 153, 274
HinfI GANTC 5 cut(s) 7, 149, 188, 429, 536
HpaII CCGG 3 cut(s) 85, 257, 837
HphI GGTGA 3 cut(s) 230, 338, 400
Hpy166II GTNNAC 3 cut(s) 153, 274, 650
Hpy188I TCNGA 7 cut(s) 27, 92, 132, 465, 631, 804, 827
Hpy188III TCNNGA 1 cut(s) 185
Hpy8I GTNNAC 3 cut(s) 153, 274, 650
Hpy99I CGWCG 2 cut(s) 254, 491
HpyAV CCTTC 2 cut(s) 328, 581
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4IV ACGT 2 cut(s) 252, 718
HpyCH4V TGCA 5 cut(s) 213, 399, 641, 716, 771
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 291, 300, 692, 701
HpyF3I CTNAG 7 cut(s) 26, 177, 229, 464, 597, 630, 705
HpySE526I ACGT 2 cut(s) 252, 718
Hsp92II CATG 4 cut(s) 356, 544, 623, 775
HspAI GCGC 4 cut(s) 32, 102, 591, 617
Ksp22I TGATCA 1 cut(s) 543
Kzo9I GATC 3 cut(s) 87, 460, 543
LmnI GCTCC 2 cut(s) 555, 787
Lsp1109I GCAGC 3 cut(s) 197, 701, 704
LweI GCATC 2 cut(s) 298, 373
MaeI CTAG 1 cut(s) 42
MaeII ACGT 2 cut(s) 252, 718
MaeIII GTNAC 3 cut(s) 164, 218, 358
MalI GATC 3 cut(s) 89, 462, 545
MboI GATC 3 cut(s) 87, 460, 543
MboII GAAGA 5 cut(s) 340, 360, 763, 814, 817
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 784
MluCI AATT 4 cut(s) 313, 364, 657, 756
MlyI GAGTC 2 cut(s) 158, 438
MnlI CCTC 7 cut(s) 21, 26, 172, 283, 292, 592, 645
MseI TTAA 1 cut(s) 759
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 3 cut(s) 85, 257, 837
MspR9I CCNGG 4 cut(s) 235, 258, 426, 554
MvaI CCWGG 3 cut(s) 235, 426, 554
MwoI GCNNNNNNNGC 5 cut(s) 38, 291, 300, 692, 701
NciI CCSGG 1 cut(s) 258
NdeII GATC 3 cut(s) 87, 460, 543
NlaIII CATG 4 cut(s) 356, 544, 623, 775
NlaIV GGNNCC 3 cut(s) 451, 558, 835
NmuCI GTSAC 1 cut(s) 218
NspI RCATGY 2 cut(s) 623, 775
PaeI GCATGC 1 cut(s) 623
PfeI GAWTC 3 cut(s) 7, 188, 536
PfoI TCCNGGA 1 cut(s) 552
PkrI GCNGC 5 cut(s) 55, 212, 688, 691, 694
PleI GAGTC 2 cut(s) 157, 437
PmaCI CACGTG 1 cut(s) 719
PmlI CACGTG 1 cut(s) 719
PpsI GAGTC 2 cut(s) 157, 437
Ppu21I YACGTR 1 cut(s) 719
Psp6I CCWGG 3 cut(s) 233, 424, 552
PspCI CACGTG 1 cut(s) 719
PspGI CCWGG 3 cut(s) 233, 424, 552
PspN4I GGNNCC 3 cut(s) 451, 558, 835
PspPI GGNCC 3 cut(s) 422, 680, 834
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 1 cut(s) 569
RsaNI GTAC 1 cut(s) 568
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 5 cut(s) 54, 211, 687, 690, 693
Sau3AI GATC 3 cut(s) 87, 460, 543
Sau96I GGNCC 3 cut(s) 422, 680, 834
SchI GAGTC 2 cut(s) 158, 438
ScrFI CCNGG 4 cut(s) 235, 258, 426, 554
SduI GDGCHC 1 cut(s) 784
SfaNI GCATC 2 cut(s) 298, 373
SfcI CTRYAG 1 cut(s) 48
SinI GGWCC 1 cut(s) 834
SphI GCATGC 1 cut(s) 623
Sse9I AATT 4 cut(s) 313, 364, 657, 756
SsiI CCGC 2 cut(s) 53, 687
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 4 cut(s) 233, 256, 424, 552
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 1 cut(s) 97
TaiI ACGT 2 cut(s) 255, 721
TaqI TCGA 3 cut(s) 109, 184, 249
TasI AATT 4 cut(s) 313, 364, 657, 756
TauI GCSGC 2 cut(s) 56, 689
TfiI GAWTC 3 cut(s) 7, 188, 536
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TscAI CASTG 4 cut(s) 181, 418, 567, 702
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 3 cut(s) 210, 689, 692
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 2 cut(s) 489, 521
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 4 cut(s) 181, 418, 567, 702
VpaK11BI GGWCC 1 cut(s) 834
XceI RCATGY 2 cut(s) 623, 775
XspI CTAG 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.