RchiOBHm_Chr4g0426031

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008613
Physical Location & Seq
Forward (+)
51545625 .. 51546098
474 bp
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UTR
Exon/CDS
Intron
PRQ39511

Sequence Viewer

Length: 474 bp
ATGAGTTATTGGCCGGATGGTCTGTTCAAGGTGAACAGATTCGAAGAAGCATTTGGACTTGTCAGGGAGATAGATGAAAAGGGACTGAAACTGAATTTGGTAACATACAACACCATTTTGAATGGTTTTTGTCGCACTGGGATGACCAACGAGGCTATGCAGGTTTTTAGCAACATGCTAACAAGGGGAACAAAGCCTGATGCCATTACATATAACATACTGATATATTTCTATTGCAAGCAAGGCAGGATAAAGACTGCTATTCAGCTTTTCAACAGTACTGGTGCAGCAAAGGAGTGGTGCCCGGATGTAATAGCTTACACTAGTCTTCTAAGTGTGATTTGTAACTCGGTAGGTCTAGACCAGGCCATGGTTTATCTTGATAAGATGATAAGGGAAGGTATCTACCCCAACATTGGCACATGGAAGCCAATCTACATACTGGATGATATTCTTCGTGAAGGTTCTTCGTGA

Protein Analysis

157

Amino Acids

17.86

Weight (kDa)

6.27

Isoelectric Point (pI)

18.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 6 - 45 1.2e-09 PPR repeat family
PPR_3 PF13812 20 - 79 2e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 27 - 59 1.5e-12 PPR repeat
PPR_2 PF13041 32 - 75 4e-17 PPR repeat family
PPR PF01535 34 - 63 2.1e-08 PPR repeat
PPR_1 PF12854 62 - 92 9.3e-11 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 151
AccB1I GGYRCC 1 cut(s) 300
AccB7I CCANNNNNTGG 1 cut(s) 370
AcoI YGGCCR 1 cut(s) 11
AcsI RAATTY 1 cut(s) 94
AfaI GTAC 1 cut(s) 280
AfiI CCNNNNNNNGG 2 cut(s) 370, 416
AgsI TTSAA 3 cut(s) 28, 121, 274
AhlI ACTAGT 1 cut(s) 323
AjnI CCWGG 1 cut(s) 363
AjuI GAANNNNNNNTTGG 4 cut(s) 36, 68, 80, 112
AloI GAACNNNNNNTCC 2 cut(s) 8, 40
AluBI AGCT 2 cut(s) 268, 317
AluI AGCT 2 cut(s) 268, 317
AoxI GGCC 2 cut(s) 11, 366
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 94
Asp700I GAANNNNTTC 1 cut(s) 38
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 1 cut(s) 43
AsuII TTCGAA 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 305
BanI GGYRCC 1 cut(s) 300
BarI GAAGNNNNNNTAC 2 cut(s) 419, 451
BbsI GAAGAC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 299
BccI CCATC 1 cut(s) 11
BciT130I CCWGG 1 cut(s) 365
BcnI CCSGG 1 cut(s) 305
BcuI ACTAGT 1 cut(s) 323
BfaI CTAG 2 cut(s) 324, 359
BfuAI ACCTGC 1 cut(s) 151
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 2 cut(s) 305, 365
BmiI GGNNCC 1 cut(s) 302
BmrFI CCNGG 2 cut(s) 305, 365
BmrI ACTGGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 190
BmuI ACTGGG 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 42
BpuMI CCSGG 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 2 cut(s) 370, 416
Bse1I ACTGG 3 cut(s) 142, 286, 447
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 4 cut(s) 22, 147, 313, 451
BseLI CCNNNNNNNGG 2 cut(s) 370, 416
BseNI ACTGG 3 cut(s) 142, 286, 447
BseSI GKGCMC 1 cut(s) 305
BseXI GCAGC 1 cut(s) 299
BsgI GTGCAG 1 cut(s) 306
BshFI GGCC 2 cut(s) 13, 368
BshNI GGYRCC 1 cut(s) 300
BsiSI CCGG 2 cut(s) 14, 305
BslFI GGGAC 1 cut(s) 96
BslI CCNNNNNNNGG 2 cut(s) 370, 416
BsmFI GGGAC 1 cut(s) 96
BsnI GGCC 2 cut(s) 13, 368
Bsp119I TTCGAA 1 cut(s) 42
Bsp1286I GDGCHC 1 cut(s) 305
Bsp19I CCATGG 1 cut(s) 369
BspANI GGCC 2 cut(s) 13, 368
BspLI GGNNCC 1 cut(s) 302
BspMI ACCTGC 1 cut(s) 151
BspT104I TTCGAA 1 cut(s) 42
BspT107I GGYRCC 1 cut(s) 300
BsrI ACTGG 3 cut(s) 142, 286, 447
BssECI CCNNGG 1 cut(s) 369
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 1 cut(s) 278
BstBI TTCGAA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 239
BstDEI CTNAG 1 cut(s) 332
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 4 cut(s) 22, 147, 313, 451
BstMWI GCNNNNNNNGC 1 cut(s) 243
BstNI CCWGG 1 cut(s) 365
BstNSI RCATGY 1 cut(s) 178
BstSCI CCNGG 2 cut(s) 303, 363
BstSLI GKGCMC 1 cut(s) 305
BstV1I GCAGC 1 cut(s) 299
BstV2I GAAGAC 1 cut(s) 320
BsuRI GGCC 2 cut(s) 13, 368
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 4 cut(s) 22, 147, 313, 451
BtsIMutI CAGTG 1 cut(s) 135
BveI ACCTGC 1 cut(s) 151
Cac8I GCNNGC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 279
CviAII CATG 3 cut(s) 175, 370, 423
CviJI RGCY 7 cut(s) 13, 155, 196, 268, 317, 368, 430
CviKI_1 RGCY 7 cut(s) 13, 155, 196, 268, 317, 368, 430
CviQI GTAC 1 cut(s) 279
DdeI CTNAG 1 cut(s) 332
EaeI YGGCCR 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 369
EcoRII CCWGG 1 cut(s) 363
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 3 cut(s) 178, 373, 426
FaqI GGGAC 1 cut(s) 96
FatI CATG 3 cut(s) 174, 369, 422
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 4 cut(s) 29, 154, 320, 458
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 2 cut(s) 324, 359
GluI GCNGC 1 cut(s) 288
HaeIII GGCC 2 cut(s) 13, 368
HapII CCGG 2 cut(s) 14, 305
Hin1II CATG 3 cut(s) 178, 373, 426
HinfI GANTC 1 cut(s) 39
HpaII CCGG 2 cut(s) 14, 305
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 1 cut(s) 34
Hpy188III TCNNGA 4 cut(s) 359, 380, 458, 471
Hpy8I GTNNAC 1 cut(s) 34
HpyAV CCTTC 2 cut(s) 392, 455
HpyCH4III ACNGT 1 cut(s) 278
HpyCH4V TGCA 3 cut(s) 160, 237, 287
HpyF10VI GCNNNNNNNGC 1 cut(s) 243
HpyF3I CTNAG 1 cut(s) 332
Hsp92II CATG 3 cut(s) 178, 373, 426
Lsp1109I GCAGC 1 cut(s) 299
LweI GCATC 1 cut(s) 190
MaeI CTAG 2 cut(s) 324, 359
MaeIII GTNAC 2 cut(s) 100, 344
MboII GAAGA 4 cut(s) 56, 320, 446, 459
MhlI GDGCHC 1 cut(s) 305
MluCI AATT 1 cut(s) 94
MnlI CCTC 1 cut(s) 145
MroXI GAANNNNTTC 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 140
MspI CCGG 2 cut(s) 14, 305
MspR9I CCNGG 2 cut(s) 305, 365
MvaI CCWGG 1 cut(s) 365
MwoI GCNNNNNNNGC 1 cut(s) 243
NciI CCSGG 1 cut(s) 305
NcoI CCATGG 1 cut(s) 369
NlaIII CATG 3 cut(s) 178, 373, 426
NlaIV GGNNCC 1 cut(s) 302
NspI RCATGY 1 cut(s) 178
NspV TTCGAA 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 39
PflMI CCANNNNNTGG 1 cut(s) 370
PkrI GCNGC 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 1 cut(s) 302
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
RseI CAYNNNNRTG 1 cut(s) 140
SatI GCNGC 1 cut(s) 288
ScaI AGTACT 1 cut(s) 280
ScrFI CCNGG 2 cut(s) 305, 365
SduI GDGCHC 1 cut(s) 305
SetI ASST 7 cut(s) 33, 165, 270, 319, 358, 403, 466
SfaNI GCATC 1 cut(s) 190
SfuI TTCGAA 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 140
SpeI ACTAGT 1 cut(s) 323
Sse9I AATT 1 cut(s) 94
SspMI CTAG 2 cut(s) 324, 359
StyD4I CCNGG 2 cut(s) 303, 363
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 278
TaqI TCGA 1 cut(s) 42
TasI AATT 1 cut(s) 94
TatI WGTACW 1 cut(s) 278
TfiI GAWTC 1 cut(s) 39
TscAI CASTG 1 cut(s) 142
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 1 cut(s) 90
TspRI CASTG 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 370
XapI RAATTY 1 cut(s) 94
XbaI TCTAGA 1 cut(s) 358
XceI RCATGY 1 cut(s) 178
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 2 cut(s) 324, 359
ZrmI AGTACT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.