Rh4BG271100

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008613
Physical Location & Seq
Forward (+)
44456768 .. 44457304
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG271100.1

Sequence Viewer

Length: 537 bp
ATGAGAGAAAATGGGTGTTTGCCCAACATTACGACATATAATGAGTTATTGGATGGTCTGTTCAAGGTGAACAGATTCGAAGAAGCATTTGGACTTGTCAGGGAGATAGATGAAAAGGGACTGAAACTGAATTTGGTAACATACAACACCATTTTGAATGGTTTTTGTCGTGCCGGGATGACTAACGAGGCTATGCAGGTTTTTAGCAACATGCTAACAAGGGGAACAAAGCCTGATGCCATTACATATAACATACTGATATATTTCTATTGCAAGCAAGGCAGGATAAAGACTGCTATTCAGTTTTTCAACAGTACTGGTGCAGCAAAAGAGTGGTGCCCTGATGTAATAGCTTACACTAGTCTTCTAAGTGGGATTTGTAACTCGGTAGGTCTAGACCAGGCCATGGTTTATCTTGATAAGATGATAAGGGAAGCCAACATTGGCACATGGACTGTGTTGGTGCGGTGCTTCTTTAGCAGCTTAGGTCATTTGGGGCCAATCTACATACTGGATGATATTCTTCGTGAAGGATAA

Protein Analysis

178

Amino Acids

20.08

Weight (kDa)

5.86

Isoelectric Point (pI)

17.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 1 - 102 3.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 4 - 35 6e-07 PPR repeat
PPR_2 PF13041 8 - 57 4e-15 PPR repeat family
PPR_3 PF13812 32 - 91 2.4e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 39 - 71 9.2e-14 PPR repeat
PPR_2 PF13041 44 - 92 6.1e-18 PPR repeat family
PPR PF01535 46 - 75 1.1e-08 PPR repeat
PPR_1 PF12854 75 - 104 2.5e-10 PPR repeat
PPR_2 PF13041 78 - 104 1.4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 187
AccB1I GGYRCC 1 cut(s) 336
AccB7I CCANNNNNTGG 1 cut(s) 406
AciI CCGC 1 cut(s) 466
AcsI RAATTY 1 cut(s) 130
AfaI GTAC 1 cut(s) 316
AfiI CCNNNNNNNGG 1 cut(s) 406
AgsI TTSAA 3 cut(s) 64, 157, 310
AhlI ACTAGT 1 cut(s) 359
AjnI CCWGG 1 cut(s) 399
AjuI GAANNNNNNNTTGG 6 cut(s) 72, 104, 116, 148, 426, 458
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
AluBI AGCT 2 cut(s) 353, 483
AluI AGCT 2 cut(s) 353, 483
AoxI GGCC 2 cut(s) 402, 497
ApeKI GCWGC 2 cut(s) 323, 480
ApoI RAATTY 1 cut(s) 130
Asp700I GAANNNNTTC 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 497
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 79
AsuII TTCGAA 1 cut(s) 78
BaeGI GKGCMC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 356
BbvI GCAGC 2 cut(s) 335, 492
BccI CCATC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 401
BcnI CCSGG 1 cut(s) 175
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 2 cut(s) 360, 395
BfuAI ACCTGC 1 cut(s) 187
BisI GCNGC 2 cut(s) 324, 481
BlsI GCNGC 2 cut(s) 325, 482
BmcAI AGTACT 1 cut(s) 316
Bme1390I CCNGG 2 cut(s) 175, 401
BmgT120I GGNCC 1 cut(s) 497
BmiI GGNNCC 2 cut(s) 338, 498
BmrFI CCNGG 2 cut(s) 175, 401
BmsI GCATC 1 cut(s) 226
BpiI GAAGAC 1 cut(s) 356
Bpu10I CCTNAGC 1 cut(s) 484
Bpu14I TTCGAA 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 405
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse1I ACTGG 2 cut(s) 322, 516
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 3 cut(s) 58, 183, 520
BseLI CCNNNNNNNGG 1 cut(s) 406
BseNI ACTGG 2 cut(s) 322, 516
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 2 cut(s) 335, 492
BsgI GTGCAG 1 cut(s) 342
BshFI GGCC 2 cut(s) 404, 499
BshNI GGYRCC 1 cut(s) 336
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 406
BsmFI GGGAC 1 cut(s) 132
BsnI GGCC 2 cut(s) 404, 499
Bsp119I TTCGAA 1 cut(s) 78
Bsp1286I GDGCHC 1 cut(s) 341
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 1 cut(s) 466
BspANI GGCC 2 cut(s) 404, 499
BspLI GGNNCC 2 cut(s) 338, 498
BspMI ACCTGC 1 cut(s) 187
BspT104I TTCGAA 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 336
BsrI ACTGG 2 cut(s) 322, 516
BssECI CCNNGG 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 2 cut(s) 314, 457
BstBI TTCGAA 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 275
BstDEI CTNAG 2 cut(s) 368, 484
BstDSI CCRYGG 1 cut(s) 405
BstF5I GGATG 3 cut(s) 58, 183, 520
BstMWI GCNNNNNNNGC 2 cut(s) 279, 477
BstNI CCWGG 1 cut(s) 401
BstNSI RCATGY 1 cut(s) 214
BstSCI CCNGG 2 cut(s) 173, 399
BstSLI GKGCMC 1 cut(s) 341
BstV1I GCAGC 2 cut(s) 335, 492
BstV2I GAAGAC 1 cut(s) 356
BsuRI GGCC 2 cut(s) 404, 499
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 3 cut(s) 58, 183, 520
BveI ACCTGC 1 cut(s) 187
Cac8I GCNNGC 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 497
Csp6I GTAC 1 cut(s) 315
CviAII CATG 3 cut(s) 211, 406, 450
CviJI RGCY 7 cut(s) 191, 232, 353, 404, 437, 483, 499
CviKI_1 RGCY 7 cut(s) 191, 232, 353, 404, 437, 483, 499
CviQI GTAC 1 cut(s) 315
DdeI CTNAG 2 cut(s) 368, 484
Eco130I CCWWGG 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 399
EcoT14I CCWWGG 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 3 cut(s) 214, 409, 453
FaqI GGGAC 1 cut(s) 132
FatI CATG 3 cut(s) 210, 405, 449
Fnu4HI GCNGC 2 cut(s) 324, 481
FokI GGATG 3 cut(s) 65, 190, 527
Fsp4HI GCNGC 2 cut(s) 324, 481
FspBI CTAG 2 cut(s) 360, 395
GluI GCNGC 2 cut(s) 324, 481
HaeIII GGCC 2 cut(s) 404, 499
HapII CCGG 1 cut(s) 174
Hin1II CATG 3 cut(s) 214, 409, 453
HinfI GANTC 1 cut(s) 75
HpaII CCGG 1 cut(s) 174
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 1 cut(s) 70
Hpy188III TCNNGA 3 cut(s) 395, 416, 527
Hpy8I GTNNAC 1 cut(s) 70
HpyAV CCTTC 1 cut(s) 524
HpyCH4III ACNGT 2 cut(s) 314, 457
HpyCH4V TGCA 3 cut(s) 196, 273, 323
HpyF10VI GCNNNNNNNGC 2 cut(s) 279, 477
HpyF3I CTNAG 2 cut(s) 368, 484
Hsp92II CATG 3 cut(s) 214, 409, 453
Lsp1109I GCAGC 2 cut(s) 335, 492
LweI GCATC 1 cut(s) 226
MaeI CTAG 2 cut(s) 360, 395
MaeIII GTNAC 2 cut(s) 136, 380
MboII GAAGA 3 cut(s) 92, 356, 515
MhlI GDGCHC 1 cut(s) 341
MluCI AATT 1 cut(s) 130
MnlI CCTC 1 cut(s) 181
MroXI GAANNNNTTC 1 cut(s) 74
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 175, 401
MvaI CCWGG 1 cut(s) 401
MwoI GCNNNNNNNGC 2 cut(s) 279, 477
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 405
NlaIII CATG 3 cut(s) 214, 409, 453
NlaIV GGNNCC 2 cut(s) 338, 498
NspI RCATGY 1 cut(s) 214
NspV TTCGAA 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 1 cut(s) 75
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 2 cut(s) 325, 482
Psp6I CCWGG 1 cut(s) 399
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 2 cut(s) 338, 498
PspPI GGNCC 1 cut(s) 497
RsaI GTAC 1 cut(s) 316
RsaNI GTAC 1 cut(s) 315
SatI GCNGC 2 cut(s) 324, 481
Sau96I GGNCC 1 cut(s) 497
ScaI AGTACT 1 cut(s) 316
ScrFI CCNGG 2 cut(s) 175, 401
SduI GDGCHC 1 cut(s) 341
SetI ASST 6 cut(s) 69, 201, 355, 394, 485, 490
SfaNI GCATC 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 78
SpeI ACTAGT 1 cut(s) 359
Sse9I AATT 1 cut(s) 130
SsiI CCGC 1 cut(s) 466
SspMI CTAG 2 cut(s) 360, 395
StyD4I CCNGG 2 cut(s) 173, 399
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 2 cut(s) 314, 457
TaqI TCGA 1 cut(s) 78
TasI AATT 1 cut(s) 130
TatI WGTACW 1 cut(s) 314
TfiI GAWTC 1 cut(s) 75
TseI GCWGC 2 cut(s) 323, 480
TspDTI ATGAA 1 cut(s) 126
Van91I CCANNNNNTGG 1 cut(s) 406
XapI RAATTY 1 cut(s) 130
XbaI TCTAGA 1 cut(s) 394
XceI RCATGY 1 cut(s) 214
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 2 cut(s) 360, 395
ZrmI AGTACT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.