RchiOBHm_Chr5g0047331

Thioredoxin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007967
Physical Location & Seq
Forward (+)
43711204 .. 43712134
931 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32526

Sequence Viewer

Length: 378 bp
ATGGTTACAGAAGATCACAAGTTGAAGAATCATGAGTTACCTATTGACGACGTTCTCGCCAAATCAGCCCTTGACGTCTCCAAGTTCGCAACCGTGGCACTCGTCGATGTCGATTCCGACGACGTTCAGGTCTACCCCCATTACTTCGACATCACTTTGATTCCCTCCACCATTTTCTTCCACAACGCCCATCACATCAAGATGGATTCCGGAACTGCTGATCACACCAAATGGATCGGCGCGTTTCGGACGAAGCAGGACTTCATTGATGTTGTCGAGGCTATTTTCAGGGGAGCGATGAAAGGGAAGCTAATTGTGAACTGCCCATTGCCGCCGGAACGAATTCCGAGATACCAGTTGTACTATAAGGATGTCTAG

Protein Analysis

125

Amino Acids

14.3

Weight (kDa)

5.92

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIM1 PF02966 13 - 111 2.5e-33 Mitosis protein DIM1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 128
AatII GACGTC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 132
AccII CGCG 1 cut(s) 242
AccIII TCCGGA 1 cut(s) 209
AciI CCGC 1 cut(s) 332
AclWI GGATC 1 cut(s) 242
AcsI RAATTY 1 cut(s) 342
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 1 cut(s) 362
AgsI TTSAA 1 cut(s) 25
AluBI AGCT 1 cut(s) 310
AluI AGCT 1 cut(s) 310
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 209
ApoI RAATTY 1 cut(s) 342
Asp700I GAANNNNTTC 1 cut(s) 342
AspLEI GCGC 1 cut(s) 242
BccI CCATC 2 cut(s) 196, 198
BclI TGATCA 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 1 cut(s) 376
BisI GCNGC 1 cut(s) 332
BlsI GCNGC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 326
BseAI TCCGGA 1 cut(s) 209
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 1 cut(s) 376
BseMI GCAATG 1 cut(s) 326
BseNI ACTGG 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 242
BsiSI CCGG 2 cut(s) 210, 335
BsmAI GTCTC 1 cut(s) 82
BsmBI CGTCTC 1 cut(s) 82
Bsp13I TCCGGA 1 cut(s) 209
Bsp143I GATC 3 cut(s) 13, 220, 234
BspACI CCGC 1 cut(s) 332
BspEI TCCGGA 1 cut(s) 209
BspFNI CGCG 1 cut(s) 242
BspHI TCATGA 1 cut(s) 31
BspPI GGATC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 326
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 3 cut(s) 13, 220, 234
BssNI GRCGYC 1 cut(s) 75
Bst4CI ACNGT 1 cut(s) 94
BstACI GRCGYC 1 cut(s) 75
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 1 cut(s) 376
BstFNI CGCG 1 cut(s) 242
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 3 cut(s) 16, 223, 237
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 3 cut(s) 13, 220, 234
BstMWI GCNNNNNNNGC 2 cut(s) 65, 95
BstUI CGCG 1 cut(s) 242
BtgI CCRYGG 1 cut(s) 93
BtgZI GCGATG 1 cut(s) 311
BtsCI GGATG 1 cut(s) 376
CciI TCATGA 1 cut(s) 31
CfoI GCGC 1 cut(s) 242
Csp6I GTAC 1 cut(s) 361
CviAII CATG 1 cut(s) 32
CviJI RGCY 3 cut(s) 68, 281, 310
CviKI_1 RGCY 3 cut(s) 68, 281, 310
CviQI GTAC 1 cut(s) 361
DpnI GATC 3 cut(s) 15, 222, 236
DpnII GATC 3 cut(s) 13, 220, 234
DrdI GACNNNNNNGTC 1 cut(s) 128
DseDI GACNNNNNNGTC 1 cut(s) 128
EcoRI GAATTC 1 cut(s) 342
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 1 cut(s) 35
FaiI YATR 2 cut(s) 33, 366
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FatI CATG 1 cut(s) 31
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 1 cut(s) 332
Fsp4HI GCNGC 1 cut(s) 332
FspBI CTAG 1 cut(s) 376
GlaI GCGC 1 cut(s) 241
GluI GCNGC 1 cut(s) 332
HapII CCGG 2 cut(s) 210, 335
HhaI GCGC 1 cut(s) 242
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 1 cut(s) 35
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HinfI GANTC 4 cut(s) 28, 113, 160, 206
HpaII CCGG 2 cut(s) 210, 335
Hpy166II GTNNAC 2 cut(s) 133, 319
Hpy188I TCNGA 3 cut(s) 118, 249, 348
Hpy188III TCNNGA 3 cut(s) 32, 199, 210
Hpy8I GTNNAC 2 cut(s) 133, 319
Hpy99I CGWCG 4 cut(s) 53, 107, 122, 125
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 3 cut(s) 51, 75, 123
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 95
HpySE526I ACGT 3 cut(s) 51, 75, 123
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 1 cut(s) 35
HspAI GCGC 1 cut(s) 240
Kpn2I TCCGGA 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 3 cut(s) 13, 220, 234
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 6 cut(s) 113, 223, 242, 274, 348, 368
MaeI CTAG 1 cut(s) 376
MaeII ACGT 3 cut(s) 51, 75, 123
MaeIII GTNAC 2 cut(s) 4, 36
MalI GATC 3 cut(s) 15, 222, 236
MboI GATC 3 cut(s) 13, 220, 234
MboII GAAGA 3 cut(s) 23, 37, 169
MluCI AATT 2 cut(s) 312, 342
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 2 cut(s) 175, 271
MroI TCCGGA 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 200
MspI CCGG 2 cut(s) 210, 335
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 2 cut(s) 65, 95
NdeII GATC 3 cut(s) 13, 220, 234
NlaIII CATG 1 cut(s) 35
PagI TCATGA 1 cut(s) 31
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 117
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 4 cut(s) 28, 113, 160, 206
PkrI GCNGC 1 cut(s) 333
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 1 cut(s) 200
SatI GCNGC 1 cut(s) 332
Sau3AI GATC 3 cut(s) 13, 220, 234
SetI ASST 6 cut(s) 43, 54, 78, 126, 132, 312
SmiMI CAYNNNNRTG 1 cut(s) 200
Sse9I AATT 2 cut(s) 312, 342
SsiI CCGC 1 cut(s) 332
SspMI CTAG 1 cut(s) 376
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 3 cut(s) 54, 78, 126
TaqI TCGA 4 cut(s) 105, 111, 147, 276
TasI AATT 2 cut(s) 312, 342
TatI WGTACW 1 cut(s) 360
TauI GCSGC 1 cut(s) 334
TfiI GAWTC 4 cut(s) 28, 113, 160, 206
TspDTI ATGAA 2 cut(s) 253, 314
XapI RAATTY 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 1 cut(s) 376
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.