Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004818

Physical Location & Seq

Genomic Coordinates Reverse (-)

88817513 .. 88821752

Gene Structure 4240 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ35727

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1482 bp

ATGATGCTTGAAAGACGGATTGCAGGCCTGCTATTGCTCCTAGTCGGCATGTTTACCAATGGATTAGGTGCATTCGTAGGGGTAAACATTGGTACTGATGTTTCAGACATTCCAGCTGGGACTGAAATAGTTGCGATCCTTATAGCCCATCAAATCACACATGTTCGCCTTTATGATGCTGATGCTCACATGCTGAGAGCCCTTTCAAACAGTGGTATTGAAGTAGTGGTAGGTATTACAAATGAGGAGGTCCTAGGGATTGGAGAGTCTCCATCAGTAGCAGCAGCCTGGGTTAATAAAAATGTCGCACCTTACTTACCTTCCACCAACATTACAGCCATTGCTGTTGGCAGTGAGGTTCTTACCCAAATTCCTCATGCTTCGCCAGTTTTGGTTTCTGCTATGAATTACATTCACAAAGCCCTGGTTGCTACAAATTTAAACTTCCAGGTCAAAGTTTCAACTCCACAATCCATGGATGTAATCCCTAAGGCTTTCCCCCCCTCCACTGCTACCTTTGATCCATCATGGAACTCTACAGTTTTCCAAATCCTTCAGTTTTTGAAAAACACAAATTCCTATTACATGTTAAATGCATATCCTTATTATGGTTACACCAAGGGTGATGGAATTTTCCCAATTGATTATGCTCTTTTTCAACCACTTCCTTCTGTCAAGCAGATTGTTGATCCAAACACTTTATCCCACTATAACAGCATGTTTGATGCTATGGTTGATGCTACCTATTATTCTATAGAGGCCCTCAATTTTACTGGGATTTCTGTTGTTGTTACGGAGAGTGGTTGGCCATCTTTGGGTGGATCCAATGAACCTGATGCGAATGTGGAAAATGCTCAGACATACACAAACAACTTGATTCGGCGAGTTTTGAATGATTCAGGTCCACCAAGCAAGCCAACTTTTCCTATCAACACTTACATTTATGAGTTGTTCAATGAAGATAAGAGGCCAGGGCCAGTGTCAGAGAAAAATTGGGGCGTGCTCTTCACTAATGGGTCTACTGTTTATCCTTTAACTTGGGGTACTTCCACTCCAACTGGAAATTCTTCAGGGTTGTTCTGTGTGGCGCAAACTGATGCAGATCCTGATAAGTTGCAAGATGGCTTAAACTGGGCTTGTGGGCAAGGCCAAGCTGATTGCACTCCCATTCAACAAGGGCATGGGTGTTTTCTTCCCAATACACTTGTAAATCATGCTTCATATGCGTATAATGATTACTATCAAAGAATGCAGAGTGTCGGTGGAACATGCGACTTTGATGGCACGGCTAAGACAACTACTGTTGATCCCAGTTATGGATCTTGCAAATTTGCAAGTTCTAATTCAAGTACAACCGGGGCACTTACACCAATCTCGGCGACTGCACCTTCAAGCCCTGTTGGAGGCTGGAGTTCAAATCTGAAAGTCTCCCAACTGCAGTATCTCATACCAGCCGCATTTCTTCCATTGTTATTGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001871 GO:0003674 GO:0003824 GO:0004553 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0016787 GO:0016798 GO:0030246 GO:0030247 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00500 ko04120 map00500 map04120

Pfam Domains

Protein Families

Glyco_hydro_17 X8

Protein Analysis

493

Amino Acids

53.03

Weight (kDa)

4.82

Isoelectric Point (pI)

37.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_17	PF00332	26 - 345	2.1e-72	Glycosyl hydrolases family 17
X8	PF07983	360 - 430	2.9e-20	X8 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004818)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13560 AT3G13560 AT3G13560 AT3G13560 AT3G13560
fragaria_vesca	FvH4_3g45330 FvH4_3g45330 FvH4_3g45330 FvH4_3g45330 FvH4_3g45330 FvH4_3g45330 FvH4_3g45330
malus_domestica	MD03G1005900.v1.1 MD11G1008900.v1.1
prunus_persica	Prupe.6G005700_v2.0.a1 Prupe.6G005700_v2.0.a1 Prupe.6G005700_v2.0.a1
pyrus_communis	pycom03g00600 pycom11g00580
rosa_chinensis	RchiOBHm_Chr5g0083121
rosa_laevigata	RLG00000037012
rosa_multiflora	Rmu_co8420859.1_g000001 Rmu_sc0002900.1_g000005 Rmu_sc0029325.1_g000001
rosa_roxburghii	Rroxscaffold_1G00001390
rosa_rugosa	Rorug05G0482300
rosa_samantha	Rh5AG532000 Rh5BG559100 Rh5CG582000 Rh5DG567900
rosa_wichuraiana	Rw5G049640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1019
AciI	CCGC	1 cut(s)	1455
AclWI	GGATC	8 cut(s)	130, 515, 683, 816, 829, 1097, 1301, 1327
AcoI	YGGCCR	1 cut(s)	806
AcsI	RAATTY	6 cut(s)	369, 436, 574, 630, 1063, 1328
AcuI	CTGAAG	2 cut(s)	539, 1053
AfaI	GTAC	3 cut(s)	94, 1045, 1351
AfiI	CCNNNNNNNGG	4 cut(s)	608, 815, 1316, 1403
AflIII	ACRYGT	2 cut(s)	160, 585
AjnI	CCWGG	4 cut(s)	287, 423, 447, 970
AjuI	GAANNNNNNNTTGG	2 cut(s)	1048, 1080
AluBI	AGCT	2 cut(s)	116, 1154
AluI	AGCT	2 cut(s)	116, 1154
Alw21I	GWGCWC	1 cut(s)	1005
Alw26I	GTCTC	2 cut(s)	273, 1432
AlwI	GGATC	8 cut(s)	130, 515, 683, 816, 829, 1097, 1301, 1327
AlwNI	CAGNNNCTG	1 cut(s)	1106
AoxI	GGCC	6 cut(s)	25, 759, 806, 968, 974, 1147
ApeKI	GCWGC	2 cut(s)	281, 284
ApoI	RAATTY	6 cut(s)	369, 436, 574, 630, 1063, 1328
Asp700I	GAANNNNTTC	1 cut(s)	1066
AspA2I	CCTAGG	1 cut(s)	253
AspLEI	GCGC	1 cut(s)	1090
AspS9I	GGNCC	4 cut(s)	250, 760, 902, 974
AsuC2I	CCSGG	1 cut(s)	1357
AsuHPI	GGTGA	1 cut(s)	635
AvaII	GGWCC	2 cut(s)	250, 902
AvrII	CCTAGG	1 cut(s)	253
AxyI	CCTNAGG	1 cut(s)	489
BaeGI	GKGCMC	1 cut(s)	1363
BaeI	ACNNNNGTAYC	4 cut(s)	84, 117, 1424, 1457
BalI	TGGCCA	1 cut(s)	808
BamHI	GGATCC	1 cut(s)	821
BanII	GRGCYC	1 cut(s)	202
Bbv12I	GWGCWC	1 cut(s)	1005
BbvI	GCAGC	2 cut(s)	293, 296
BccI	CCATC	7 cut(s)	156, 280, 532, 620, 817, 1115, 1274
BceAI	ACGGC	1 cut(s)	1302
BciT130I	CCWGG	4 cut(s)	289, 425, 449, 972
BcnI	CCSGG	1 cut(s)	1357
BcoDI	GTCTC	2 cut(s)	273, 1432
BfaI	CTAG	2 cut(s)	41, 254
BfmI	CTRYAG	3 cut(s)	537, 753, 1436
BisI	GCNGC	3 cut(s)	282, 285, 1455
BlnI	CCTAGG	1 cut(s)	253
BlsI	GCNGC	3 cut(s)	283, 286, 1456
Bme1390I	CCNGG	5 cut(s)	289, 425, 449, 972, 1357
Bme18I	GGWCC	2 cut(s)	250, 902
BmgT120I	GGNCC	4 cut(s)	250, 760, 902, 974
BmiI	GGNNCC	1 cut(s)	823
BmrFI	CCNGG	5 cut(s)	289, 425, 449, 972, 1357
BmrI	ACTGGG	3 cut(s)	783, 1141, 1305
BmsI	GCATC	6 cut(s)	166, 172, 715, 727, 826, 1087
BmuI	ACTGGG	3 cut(s)	783, 1141, 1305
BpmI	CTGGAG	1 cut(s)	1429
BpuMI	CCSGG	1 cut(s)	1357
BsaBI	GATNNNNATC	2 cut(s)	1101, 1239
BsaJI	CCNNGG	7 cut(s)	253, 288, 423, 474, 618, 971, 1356
BsaXI	ACNNNNNCTCC	2 cut(s)	1036, 1066
Bsc4I	CCNNNNNNNGG	4 cut(s)	608, 815, 1316, 1403
Bse1I	ACTGG	6 cut(s)	386, 778, 977, 1063, 1136, 1311
Bse21I	CCTNAGG	1 cut(s)	489
Bse3DI	GCAATG	1 cut(s)	339
Bse8I	GATNNNNATC	2 cut(s)	1101, 1239
BseBI	CCWGG	4 cut(s)	289, 425, 449, 972
BseDI	CCNNGG	7 cut(s)	253, 288, 423, 474, 618, 971, 1356
BseGI	GGATG	1 cut(s)	484
BseJI	GATNNNNATC	2 cut(s)	1101, 1239
BseLI	CCNNNNNNNGG	4 cut(s)	608, 815, 1316, 1403
BseMI	GCAATG	1 cut(s)	339
BseMII	CTCAG	2 cut(s)	185, 869
BseNI	ACTGG	6 cut(s)	386, 778, 977, 1063, 1136, 1311
BseRI	GAGGAG	1 cut(s)	260
BseSI	GKGCMC	1 cut(s)	1363
BseXI	GCAGC	2 cut(s)	293, 296
BseYI	CCCAGC	1 cut(s)	116
BsgI	GTGCAG	1 cut(s)	1368
BshFI	GGCC	6 cut(s)	27, 761, 808, 970, 976, 1149
BsiHKAI	GWGCWC	1 cut(s)	1005
BsiSI	CCGG	1 cut(s)	1356
BslFI	GGGAC	1 cut(s)	133
BslI	CCNNNNNNNGG	4 cut(s)	608, 815, 1316, 1403
BsmAI	GTCTC	2 cut(s)	273, 1432
BsmFI	GGGAC	1 cut(s)	133
BsmI	GAATGC	2 cut(s)	71, 1254
BsnI	GGCC	6 cut(s)	27, 761, 808, 970, 976, 1149
Bsp1286I	GDGCHC	3 cut(s)	202, 1005, 1363
Bsp143I	GATC	7 cut(s)	135, 520, 688, 821, 1102, 1306, 1319
Bsp19I	CCATGG	1 cut(s)	474
BspACI	CCGC	1 cut(s)	1455
BspANI	GGCC	6 cut(s)	27, 761, 808, 970, 976, 1149
BspCNI	CTCAG	2 cut(s)	186, 868
BspLI	GGNNCC	1 cut(s)	823
BspMAI	CTGCAG	1 cut(s)	1440
BspPI	GGATC	8 cut(s)	130, 515, 683, 816, 829, 1097, 1301, 1327
BspQI	GCTCTTC	1 cut(s)	1010
BsrDI	GCAATG	1 cut(s)	339
BsrI	ACTGG	6 cut(s)	386, 778, 977, 1063, 1136, 1311
BssECI	CCNNGG	7 cut(s)	253, 288, 423, 474, 618, 971, 1356
BssMI	GATC	7 cut(s)	135, 520, 688, 821, 1102, 1306, 1319
BssT1I	CCWWGG	3 cut(s)	253, 474, 618
Bst2UI	CCWGG	4 cut(s)	289, 425, 449, 972
Bst4CI	ACNGT	4 cut(s)	212, 541, 1024, 1303
Bst6I	CTCTTC	1 cut(s)	1010
BstC8I	GCNNGC	4 cut(s)	25, 29, 914, 1001
BstDEI	CTNAG	4 cut(s)	194, 489, 855, 1290
BstDSI	CCRYGG	1 cut(s)	474
BstENI	CCTNNNNNAGG	1 cut(s)	1401
BstF5I	GGATG	1 cut(s)	484
BstHHI	GCGC	1 cut(s)	1090
BstKTI	GATC	7 cut(s)	138, 523, 691, 824, 1105, 1309, 1322
BstMAI	GTCTC	2 cut(s)	273, 1432
BstMBI	GATC	7 cut(s)	135, 520, 688, 821, 1102, 1306, 1319
BstMWI	GCNNNNNNNGC	2 cut(s)	428, 1223
BstNI	CCWGG	4 cut(s)	289, 425, 449, 972
BstNSI	RCATGY	6 cut(s)	52, 164, 193, 589, 721, 1272
BstSCI	CCNGG	5 cut(s)	287, 423, 447, 970, 1355
BstSFI	CTRYAG	3 cut(s)	537, 753, 1436
BstSLI	GKGCMC	1 cut(s)	1363
BstV1I	GCAGC	2 cut(s)	293, 296
BstX2I	RGATCY	3 cut(s)	821, 1102, 1319
BstYI	RGATCY	3 cut(s)	821, 1102, 1319
Bsu36I	CCTNAGG	1 cut(s)	489
BsuRI	GGCC	6 cut(s)	27, 761, 808, 970, 976, 1149
BtgI	CCRYGG	1 cut(s)	474
BtsCI	GGATG	1 cut(s)	484
BtsI	GCAGTG	2 cut(s)	358, 507
BtsIMutI	CAGTG	4 cut(s)	217, 358, 507, 984
Cac8I	GCNNGC	4 cut(s)	25, 29, 914, 1001
CaiI	CAGNNNCTG	1 cut(s)	1106
CfoI	GCGC	1 cut(s)	1090
Cfr13I	GGNCC	4 cut(s)	250, 760, 902, 974
Csp6I	GTAC	3 cut(s)	93, 1044, 1350
CviQI	GTAC	3 cut(s)	93, 1044, 1350
DdeI	CTNAG	4 cut(s)	194, 489, 855, 1290
DpnI	GATC	7 cut(s)	137, 522, 690, 823, 1104, 1308, 1321
DpnII	GATC	7 cut(s)	135, 520, 688, 821, 1102, 1306, 1319
DraI	TTTAAA	1 cut(s)	441
EaeI	YGGCCR	1 cut(s)	806
Eam1104I	CTCTTC	1 cut(s)	1010
EarI	CTCTTC	1 cut(s)	1010
Eco130I	CCWWGG	3 cut(s)	253, 474, 618
Eco147I	AGGCCT	1 cut(s)	27
Eco24I	GRGCYC	1 cut(s)	202
Eco47I	GGWCC	2 cut(s)	250, 902
Eco57I	CTGAAG	2 cut(s)	539, 1053
Eco81I	CCTNAGG	1 cut(s)	489
EcoNI	CCTNNNNNAGG	1 cut(s)	1401
EcoO109I	RGGNCCY	2 cut(s)	250, 760
EcoRII	CCWGG	4 cut(s)	287, 423, 447, 970
EcoT14I	CCWWGG	3 cut(s)	253, 474, 618
EcoT22I	ATGCAT	1 cut(s)	598
EcoT38I	GRGCYC	1 cut(s)	202
ErhI	CCWWGG	3 cut(s)	253, 474, 618
FaqI	GGGAC	1 cut(s)	133
FauNDI	CATATG	1 cut(s)	1222
FblI	GTMKAC	1 cut(s)	1019
Fnu4HI	GCNGC	3 cut(s)	282, 285, 1455
FokI	GGATG	1 cut(s)	491
FriOI	GRGCYC	1 cut(s)	202
Fsp4HI	GCNGC	3 cut(s)	282, 285, 1455
FspBI	CTAG	2 cut(s)	41, 254
GlaI	GCGC	1 cut(s)	1089
GluI	GCNGC	3 cut(s)	282, 285, 1455
GsaI	CCCAGC	1 cut(s)	120
GsuI	CTGGAG	1 cut(s)	1429
HaeIII	GGCC	6 cut(s)	27, 761, 808, 970, 976, 1149
HapII	CCGG	1 cut(s)	1356
HhaI	GCGC	1 cut(s)	1090
Hin6I	GCGC	1 cut(s)	1088
HinP1I	GCGC	1 cut(s)	1088
HinfI	GANTC	3 cut(s)	266, 877, 896
HpaII	CCGG	1 cut(s)	1356
HphI	GGTGA	1 cut(s)	635
Hpy166II	GTNNAC	4 cut(s)	54, 85, 905, 1020
Hpy188I	TCNGA	4 cut(s)	106, 858, 985, 1422
Hpy188III	TCNNGA	1 cut(s)	1106
Hpy8I	GTNNAC	4 cut(s)	54, 85, 905, 1020
HpyAV	CCTTC	4 cut(s)	330, 563, 678, 1398
HpyCH4III	ACNGT	4 cut(s)	212, 541, 1024, 1303
HpyF10VI	GCNNNNNNNGC	2 cut(s)	428, 1223
HpyF3I	CTNAG	4 cut(s)	194, 489, 855, 1290
HspAI	GCGC	1 cut(s)	1088
Kzo9I	GATC	7 cut(s)	135, 520, 688, 821, 1102, 1306, 1319
LguI	GCTCTTC	1 cut(s)	1010
LmnI	GCTCC	1 cut(s)	42
Lsp1109I	GCAGC	2 cut(s)	293, 296
LweI	GCATC	6 cut(s)	166, 172, 715, 727, 826, 1087
MaeI	CTAG	2 cut(s)	41, 254
MaeIII	GTNAC	2 cut(s)	611, 790
MalI	GATC	7 cut(s)	137, 522, 690, 823, 1104, 1308, 1321
MboI	GATC	7 cut(s)	135, 520, 688, 821, 1102, 1306, 1319
MboII	GAAGA	5 cut(s)	971, 997, 1059, 1184, 1454
MfeI	CAATTG	1 cut(s)	639
MflI	RGATCY	3 cut(s)	821, 1102, 1319
MhlI	GDGCHC	3 cut(s)	202, 1005, 1363
MlsI	TGGCCA	1 cut(s)	808
MluNI	TGGCCA	1 cut(s)	808
MlyI	GAGTC	1 cut(s)	275
MmeI	TCCRAC	2 cut(s)	1079, 1381
MnlI	CCTC	9 cut(s)	238, 241, 349, 384, 514, 751, 773, 960, 1397
Mox20I	TGGCCA	1 cut(s)	808
Mph1103I	ATGCAT	1 cut(s)	598
MroXI	GAANNNNTTC	1 cut(s)	1066
MscI	TGGCCA	1 cut(s)	808
MseI	TTAA	5 cut(s)	294, 440, 590, 1034, 1127
Msp20I	TGGCCA	1 cut(s)	808
MspA1I	CMGCKG	1 cut(s)	116
MspI	CCGG	1 cut(s)	1356
MspR9I	CCNGG	5 cut(s)	289, 425, 449, 972, 1357
MunI	CAATTG	1 cut(s)	639
Mva1269I	GAATGC	2 cut(s)	71, 1254
MvaI	CCWGG	4 cut(s)	289, 425, 449, 972
MwoI	GCNNNNNNNGC	2 cut(s)	428, 1223
NciI	CCSGG	1 cut(s)	1357
NcoI	CCATGG	1 cut(s)	474
NdeI	CATATG	1 cut(s)	1222
NdeII	GATC	7 cut(s)	135, 520, 688, 821, 1102, 1306, 1319
NlaIV	GGNNCC	1 cut(s)	823
NmeAIII	GCCGAG	1 cut(s)	1355
NsiI	ATGCAT	1 cut(s)	598
NspI	RCATGY	6 cut(s)	52, 164, 193, 589, 721, 1272
PceI	AGGCCT	1 cut(s)	27
PciI	ACATGT	2 cut(s)	160, 585
PciSI	GCTCTTC	1 cut(s)	1010
PctI	GAATGC	2 cut(s)	71, 1254
PdmI	GAANNNNTTC	1 cut(s)	1066
PfeI	GAWTC	2 cut(s)	877, 896
PkrI	GCNGC	3 cut(s)	283, 286, 1456
PleI	GAGTC	1 cut(s)	274
PpsI	GAGTC	1 cut(s)	274
PpuMI	RGGWCCY	1 cut(s)	250
PscI	ACATGT	2 cut(s)	160, 585
Psp5II	RGGWCCY	1 cut(s)	250
Psp6I	CCWGG	4 cut(s)	287, 423, 447, 970
PspFI	CCCAGC	1 cut(s)	116
PspGI	CCWGG	4 cut(s)	287, 423, 447, 970
PspN4I	GGNNCC	1 cut(s)	823
PspPI	GGNCC	4 cut(s)	250, 760, 902, 974
PspPPI	RGGWCCY	1 cut(s)	250
PstI	CTGCAG	1 cut(s)	1440
PstNI	CAGNNNCTG	1 cut(s)	1106
PsuI	RGATCY	3 cut(s)	821, 1102, 1319
PvuII	CAGCTG	1 cut(s)	116
RsaI	GTAC	3 cut(s)	94, 1045, 1351
RsaNI	GTAC	3 cut(s)	93, 1044, 1350
SapI	GCTCTTC	1 cut(s)	1010
SaqAI	TTAA	5 cut(s)	294, 440, 590, 1034, 1127
SatI	GCNGC	3 cut(s)	282, 285, 1455
Sau3AI	GATC	7 cut(s)	135, 520, 688, 821, 1102, 1306, 1319
Sau96I	GGNCC	4 cut(s)	250, 760, 902, 974
SchI	GAGTC	1 cut(s)	275
ScrFI	CCNGG	5 cut(s)	289, 425, 449, 972, 1357
SduI	GDGCHC	3 cut(s)	202, 1005, 1363
SfaNI	GCATC	6 cut(s)	166, 172, 715, 727, 826, 1087
SfcI	CTRYAG	3 cut(s)	537, 753, 1436
SinI	GGWCC	2 cut(s)	250, 902
SseBI	AGGCCT	1 cut(s)	27
SsiI	CCGC	1 cut(s)	1455
SspMI	CTAG	2 cut(s)	41, 254
StuI	AGGCCT	1 cut(s)	27
StyD4I	CCNGG	5 cut(s)	287, 423, 447, 970, 1355
StyI	CCWWGG	3 cut(s)	253, 474, 618
TaaI	ACNGT	4 cut(s)	212, 541, 1024, 1303
TatI	WGTACW	1 cut(s)	1349
TauI	GCSGC	1 cut(s)	1457
TfiI	GAWTC	2 cut(s)	877, 896
Tru1I	TTAA	5 cut(s)	294, 440, 590, 1034, 1127
Tru9I	TTAA	5 cut(s)	294, 440, 590, 1034, 1127
TscAI	CASTG	4 cut(s)	217, 358, 514, 984
TseI	GCWGC	2 cut(s)	281, 284
TspDTI	ATGAA	4 cut(s)	419, 843, 972, 1209
TspGWI	ACGGA	2 cut(s)	31, 809
TspRI	CASTG	4 cut(s)	217, 358, 514, 984
VpaK11BI	GGWCC	2 cut(s)	250, 902
XagI	CCTNNNNNAGG	1 cut(s)	1401
XapI	RAATTY	6 cut(s)	369, 436, 574, 630, 1063, 1328
XceI	RCATGY	6 cut(s)	52, 164, 193, 589, 721, 1272
XmaJI	CCTAGG	1 cut(s)	253
XmiI	GTMKAC	1 cut(s)	1019
XmnI	GAANNNNTTC	1 cut(s)	1066
XspI	CTAG	2 cut(s)	41, 254
Zsp2I	ATGCAT	1 cut(s)	598

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.