RchiOBHm_Chr6g0251031

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004517
Physical Location & Seq
Forward (+)
6353880 .. 6358686
4807 bp
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UTR
Exon/CDS
Intron
PRQ22504

Sequence Viewer

Length: 588 bp
ATGTCGACAAACACAAAATTCAGCGTTTACGTAGGTAGTTTGGATGAGCGGGTGAGTGATAGAGTGTTATATGATATTCTAATTCAGGCAGGGAGGGTAGTTGACCTGCACATCCCTCGAGACAAGGAAACCGACAGGCCTAAAGGTTATGCTTTTGCAGAATATGAAACTGAGGAGATTGCTGAGTATGCTGTTAGGCTTTTCTCCGGTCTTGTGACTCTCTACAATCGAACACTCAAGTTTGCGATTTCTGGGCAAGACAAGCCTTCACCTAATGTTGCTTCTACAGTAATGCCCACACCCAATTTTTCTAACAAATTGAGGTCTCCCCCTGTACCTATTAACAATGTAGAAAACTTGCAGAATTCAATGAGCTTATCAGCACCCTGCAAAATTCCAGCTTATGCAGAAACTTATCCCTCAGTGCCCATTCCCCCTGGTGTAAATAACCAATCTAATGGATTACATTTGAATGGCAACAATTTCGAGTATAGTCGAAGAGTTATTGGGGCAACATTGGATACCATTAGTCGCCCTAGGTCACGCCGCTATGATATGAGTAATCCAATTTCCTATCCCTCCTACTAA

Protein Analysis

195

Amino Acids

21.81

Weight (kDa)

8.61

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 9 - 80 4.1e-14 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 114
AccBSI CCGCTC 1 cut(s) 49
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 49, 547
AcsI RAATTY 3 cut(s) 17, 364, 393
AfaI GTAC 1 cut(s) 336
AgsI TTSAA 2 cut(s) 369, 472
AjnI CCWGG 1 cut(s) 436
AluBI AGCT 2 cut(s) 375, 401
AluI AGCT 2 cut(s) 375, 401
Alw26I GTCTC 2 cut(s) 114, 330
Ama87I CYCGRG 1 cut(s) 117
AoxI GGCC 1 cut(s) 137
ApoI RAATTY 3 cut(s) 17, 364, 393
AspA2I CCTAGG 1 cut(s) 536
AsuHPI GGTGA 2 cut(s) 64, 261
AvaI CYCGRG 1 cut(s) 117
AvrII CCTAGG 1 cut(s) 536
BaeGI GKGCMC 1 cut(s) 429
BcgI CGANNNNNNTGC 4 cut(s) 98, 132, 466, 500
BciT130I CCWGG 1 cut(s) 438
BciVI GTATCC 1 cut(s) 514
BcoDI GTCTC 2 cut(s) 114, 330
BfaI CTAG 1 cut(s) 537
BfmI CTRYAG 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 114
BfuI GTATCC 1 cut(s) 514
BisI GCNGC 1 cut(s) 547
BlnI CCTAGG 1 cut(s) 536
BlsI GCNGC 1 cut(s) 548
Bme1390I CCNGG 1 cut(s) 438
BmeT110I CYCGRG 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 221
BsaAI YACGTR 1 cut(s) 31
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 2 cut(s) 436, 536
BsaWI WCCGGW 1 cut(s) 206
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 436, 536
BseGI GGATG 2 cut(s) 49, 111
BseMII CTCAG 3 cut(s) 162, 174, 435
BseRI GAGGAG 1 cut(s) 188
BseSI GKGCMC 1 cut(s) 429
BsgI GTGCAG 1 cut(s) 92
BshFI GGCC 1 cut(s) 139
BsiHKCI CYCGRG 1 cut(s) 117
BsiSI CCGG 1 cut(s) 207
BsmAI GTCTC 2 cut(s) 114, 330
BsnI GGCC 1 cut(s) 139
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 429
BspACI CCGC 2 cut(s) 49, 547
BspANI GGCC 1 cut(s) 139
BspCNI CTCAG 3 cut(s) 163, 175, 434
BspMI ACCTGC 1 cut(s) 114
BspTNI GGTCTC 1 cut(s) 330
BsrBI CCGCTC 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 436, 536
BssT1I CCWWGG 1 cut(s) 536
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 1 cut(s) 493
BstBAI YACGTR 1 cut(s) 31
BstDEI CTNAG 3 cut(s) 171, 183, 421
BstF5I GGATG 2 cut(s) 49, 111
BstMAI GTCTC 2 cut(s) 114, 330
BstMWI GCNNNNNNNGC 2 cut(s) 188, 262
BstNI CCWGG 1 cut(s) 438
BstSCI CCNGG 1 cut(s) 436
BstSFI CTRYAG 1 cut(s) 285
BstSLI GKGCMC 1 cut(s) 429
BstSNI TACGTA 1 cut(s) 31
BstXI CCANNNNNNTGG 1 cut(s) 458
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 1 cut(s) 139
BtsCI GGATG 2 cut(s) 49, 111
BtsIMutI CAGTG 1 cut(s) 429
BveI ACCTGC 1 cut(s) 114
Csp6I GTAC 1 cut(s) 335
CviJI RGCY 5 cut(s) 139, 199, 265, 375, 401
CviKI_1 RGCY 5 cut(s) 139, 199, 265, 375, 401
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 3 cut(s) 171, 183, 421
Eam1104I CTCTTC 1 cut(s) 493
EarI CTCTTC 1 cut(s) 493
Eco105I TACGTA 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 536
Eco147I AGGCCT 1 cut(s) 139
Eco31I GGTCTC 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 117
EcoRI GAATTC 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 536
FaiI YATR 9 cut(s) 70, 72, 150, 165, 189, 405, 492, 552, 557
FauI CCCGC 1 cut(s) 42
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 547
FokI GGATG 2 cut(s) 56, 98
Fsp4HI GCNGC 1 cut(s) 547
FspBI CTAG 1 cut(s) 537
GluI GCNGC 1 cut(s) 547
HaeIII GGCC 1 cut(s) 139
HapII CCGG 1 cut(s) 207
HincII GTYRAC 2 cut(s) 6, 103
HindII GTYRAC 2 cut(s) 6, 103
HinfI GANTC 1 cut(s) 217
HpaII CCGG 1 cut(s) 207
HphI GGTGA 2 cut(s) 64, 261
Hpy166II GTNNAC 3 cut(s) 6, 28, 103
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 3 cut(s) 6, 28, 103
HpyAV CCTTC 1 cut(s) 276
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 5 cut(s) 109, 158, 361, 390, 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 262
HpyF3I CTNAG 3 cut(s) 171, 183, 421
HpySE526I ACGT 1 cut(s) 30
MaeI CTAG 1 cut(s) 537
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 2 cut(s) 214, 540
MbiI CCGCTC 1 cut(s) 49
MboII GAAGA 1 cut(s) 510
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 8 cut(s) 17, 81, 304, 317, 364, 393, 481, 567
MlyI GAGTC 1 cut(s) 211
MnlI CCTC 5 cut(s) 87, 126, 166, 315, 430
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 471
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 438
MvaI CCWGG 1 cut(s) 438
MwoI GCNNNNNNNGC 2 cut(s) 188, 262
NmuCI GTSAC 2 cut(s) 214, 540
PaeR7I CTCGAG 1 cut(s) 117
PceI AGGCCT 1 cut(s) 139
PkrI GCNGC 1 cut(s) 548
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
Ppu21I YACGTR 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
RseI CAYNNNNRTG 1 cut(s) 471
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 1 cut(s) 547
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 1 cut(s) 438
SduI GDGCHC 1 cut(s) 429
SfcI CTRYAG 1 cut(s) 285
Sfr274I CTCGAG 1 cut(s) 117
SlaI CTCGAG 1 cut(s) 117
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 2 cut(s) 117, 236
SmoI CTYRAG 2 cut(s) 117, 236
SnaBI TACGTA 1 cut(s) 31
Sse9I AATT 8 cut(s) 17, 81, 304, 317, 364, 393, 481, 567
SseBI AGGCCT 1 cut(s) 139
SsiI CCGC 2 cut(s) 49, 547
SspMI CTAG 1 cut(s) 537
StuI AGGCCT 1 cut(s) 139
StyD4I CCNGG 1 cut(s) 436
StyI CCWWGG 1 cut(s) 536
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 33
TaqI TCGA 5 cut(s) 5, 118, 229, 486, 496
TasI AATT 8 cut(s) 17, 81, 304, 317, 364, 393, 481, 567
TauI GCSGC 1 cut(s) 549
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 2 cut(s) 214, 540
Tsp45I GTSAC 2 cut(s) 214, 540
TspDTI ATGAA 1 cut(s) 180
TspRI CASTG 1 cut(s) 429
XapI RAATTY 3 cut(s) 17, 364, 393
XhoI CTCGAG 1 cut(s) 117
XmaJI CCTAGG 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.