Rorug05G0499500

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004517
Physical Location & Seq
Reverse (-)
68104823 .. 68106655
1833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0499500.1

Sequence Viewer

Length: 1713 bp
ATGGGTGCGATTGGAATCGGTCATGATGCTTGTACTCTCAGAATAATCGCAAATTGCTACTGTCATTTGAAACAAGCTGCGTTGAGTTTACCTGTCTTGGCACATTTCTTCAAACTCGGCCTTCAACCGGATGTCACCACCTTCGCCACTCTAATCAACGCCTTTCTTCTTCAGACAACTAGAGAGGAGCCCGGGACCTCAAACCCCAAAATCAATTACCTTGACTATGCCTTGAAGGTGTTCGATGAAATGCTTCAAAGGCGTCCTCTGCCTTCGGTTGTCCGTTTCACTCAGATATTGAGTCAACTTGTCAAATTGGAACACTATTCTGCAGCACAATTCTTCAAATTGGGTCGTCAACCAAATGTCACCACCTTCAACACTCTGATCAACGGCTTTGTTCTCCAAAATAGAGTGGCTGAGGCTGTACTGCTTTTCAGCAAAATGGTGGAGGGAGGTCATTGTCAGCCGGATGTGTTTACTTTCAACACACTAATAAAGGGCTTTTGCATGATTGGAAACAACAGGGCAGCTATTCAATTACTTGGGAAGATGGAAGAGAGAGGATGCGAGCCTGACATATTTACCTTTAACACCATAATCGACAGTCATTGTAAGGATACACTAGTTGATGAAGCATTGAACCTCTTCACAGAAATGATTACCAGAGGTATTGCTCCAGACGTTGTTACTTACACCTCTTTAATTCAAGGAGTCTGCAAACTAGGCGAGTGGAAAGAAGCTACAAGGCTGCTGGATGAAATGTTGAGTAAAGGTATCTCTCCGAATCTCCTCACCTTCAGTGTCCTGGTTGATACATTCCGTAAGGAAGGGATGGTTGTAGAAGCCAAAAGTGTGGTTCAATTGATGATTCAAAGAGATATTGAGCCTGATGTCATTACTTACAATTTGCTTATGGACGGTTACTGTTTGCTAGGAGAAATGGACGAGGCCAAAAAAATCTTCGATCTGATGGTTAGCAAAGGCTCCATGGTTAATGTTCAGAGTTGTACCATATTGATTAACGGATATTGTAAGGGTAAAAAAATCGATCAGGCTTACAAGATTTTCAAGGATATGCCTCTTAGGGGACTTGTTCCCGATACCATTACTTACAACACTCTTGTTGATGGTTTTTGTAAAGCAGGGAGACTACAAGAAGCAGAAAAGTTGTTCTCTGAGATGCAAGGTTGTGGTCAACTTCCAGGTGTTCAAACTTATGCTGTTATACTTGATGGCCTCTGTAATAACCAACAACTTTCTACGGCAATTGAATTACTTAGAGAGATGGAAGGCAAGAAGTTGGAACTAAATATTGTAGTTTACAATATTATCATTGAAGGTTTGTGCAAAGCTTGGAAAATGGACTCTGCAATAGATGTCTTCTCTGGTTTGTCTTCAAGAGGAATACAGCCTAATGTGAGGACATACAATATAATGATTCATGGATTTTGTAAAGGGGGCTTATTAACTGAAGCAGAAAAGTTGCTGAGAGAAATGGGAGACAAAGGCTGCTCTCCAGATGGTTGGACCTATAACATCATCATCCGAGGGTTGTTGAAAAACAATGAAACATCATGGGCTATGGGACTTATTCAAGAAATGGTGGAGTGTGGTTTCTCTGCAGATGCATCAAGTATGGAATTGATAGTTGGTTTATTGTCCAAGGATATAGTAGATCCGGCTTTGTTGCTCTTGTTGAAAGATTCATGA

Protein Analysis

570

Amino Acids

63.39

Weight (kDa)

5.5

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 118 - 150 2.7e-08 PPR repeat
PPR_2 PF13041 121 - 170 7.7e-15 PPR repeat family
PPR_3 PF13812 147 - 202 1.6e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 155 - 186 7.9e-11 PPR repeat
PPR_2 PF13041 157 - 206 6e-17 PPR repeat family
PPR PF01535 160 - 190 5.1e-06 PPR repeat
PPR_1 PF12854 188 - 220 4e-13 PPR repeat
PPR_2 PF13041 192 - 241 3.1e-18 PPR repeat family
PPR PF01535 195 - 225 1.5e-06 PPR repeat
PPR_long PF17177 206 - 315 9.2e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 216 - 274 1.6e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 223 - 255 1.7e-12 PPR repeat
PPR_2 PF13041 227 - 274 8.4e-16 PPR repeat family
PPR PF01535 230 - 260 2.5e-08 PPR repeat
PPR_2 PF13041 276 - 310 1.6e-06 PPR repeat family
PPR_1 PF12854 294 - 326 5.5e-11 PPR repeat
PPR_2 PF13041 297 - 346 1.4e-14 PPR repeat family
PPR PF01535 300 - 329 1.5e-08 PPR repeat
PPR_3 PF13812 320 - 376 1.7e-06 Pentatricopeptide repeat domain
PPR_long PF17177 320 - 438 7.9e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 333 - 381 1.1e-16 PPR repeat family
PPR PF01535 336 - 365 7.1e-06 PPR repeat
PPR_1 PF12854 338 - 360 1e-05 PPR repeat
PPR_3 PF13812 355 - 412 4.9e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 363 - 396 1e-16 PPR repeat
TPR_24 PF23276 364 - 463 7.9e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 367 - 416 1.5e-16 PPR repeat family
PPR PF01535 370 - 399 4.1e-10 PPR repeat
PPR_2 PF13041 402 - 448 1.3e-08 PPR repeat family
PPR_3 PF13812 426 - 482 1.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 435 - 462 7.8e-07 PPR repeat
PPR_2 PF13041 438 - 486 2.8e-18 PPR repeat family
PPR_3 PF13812 464 - 517 8.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 468 - 500 1.4e-14 PPR repeat
PPR PF01535 476 - 505 2.9e-09 PPR repeat
PPR_2 PF13041 477 - 521 8.4e-15 PPR repeat family
PPR_1 PF12854 503 - 536 4.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 1673
AcuI CTGAAG 3 cut(s) 155, 784, 1494
AcyI GRCGYC 1 cut(s) 262
AfaI GTAC 3 cut(s) 34, 429, 1012
AfiI CCNNNNNNNGG 2 cut(s) 127, 1088
AhlI ACTAGT 1 cut(s) 625
AjnI CCWGG 2 cut(s) 807, 1204
AjuI GAANNNNNNNTTGG 4 cut(s) 947, 979, 1635, 1667
AluBI AGCT 4 cut(s) 77, 533, 743, 1355
AluI AGCT 4 cut(s) 77, 533, 743, 1355
Alw26I GTCTC 2 cut(s) 1144, 1497
AlwI GGATC 1 cut(s) 1673
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 3 cut(s) 118, 951, 1237
ApeKI GCWGC 5 cut(s) 77, 332, 530, 751, 1512
Asp700I GAANNNNTTC 3 cut(s) 239, 252, 647
AspS9I GGNCC 2 cut(s) 195, 1530
AsuC2I CCSGG 2 cut(s) 192, 193
AsuHPI GGTGA 3 cut(s) 127, 361, 787
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 2 cut(s) 195, 1530
BaeI ACNNNNGTAYC 2 cut(s) 612, 645
BanII GRGCYC 1 cut(s) 192
BbsI GAAGAC 2 cut(s) 1375, 1389
BbvCI CCTCAGC 1 cut(s) 420
BbvI GCAGC 5 cut(s) 64, 344, 542, 738, 1499
BccI CCATC 7 cut(s) 547, 829, 967, 1124, 1229, 1282, 1517
BceAI ACGGC 2 cut(s) 409, 1281
BciT130I CCWGG 2 cut(s) 809, 1206
BciVI GTATCC 1 cut(s) 613
BclI TGATCA 1 cut(s) 387
BcnI CCSGG 2 cut(s) 192, 193
BcoDI GTCTC 2 cut(s) 1144, 1497
BcuI ACTAGT 1 cut(s) 625
BfaI CTAG 4 cut(s) 180, 626, 725, 935
BfmI CTRYAG 2 cut(s) 330, 1623
BfuI GTATCC 1 cut(s) 613
BisI GCNGC 5 cut(s) 78, 333, 531, 752, 1513
BlsI GCNGC 5 cut(s) 79, 334, 532, 753, 1514
Bme1390I CCNGG 4 cut(s) 192, 193, 809, 1206
Bme18I GGWCC 2 cut(s) 195, 1530
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 195, 1530
BmiI GGNNCC 3 cut(s) 189, 196, 988
BmrFI CCNGG 4 cut(s) 192, 193, 809, 1206
BmsI GCATC 5 cut(s) 16, 557, 1173, 1618, 1640
BpiI GAAGAC 2 cut(s) 1375, 1389
BpmI CTGGAG 2 cut(s) 663, 1503
Bpu10I CCTNAGC 1 cut(s) 420
BpuMI CCSGG 2 cut(s) 192, 193
Bsa29I ATCGAT 1 cut(s) 1050
BsaBI GATNNNNATC 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 262
BsaJI CCNNGG 4 cut(s) 191, 990, 1549, 1665
BsaWI WCCGGW 1 cut(s) 127
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 1088
Bse8I GATNNNNATC 1 cut(s) 14
BseBI CCWGG 2 cut(s) 809, 1206
BseCI ATCGAT 1 cut(s) 1050
BseDI CCNNGG 4 cut(s) 191, 990, 1549, 1665
BseGI GGATG 6 cut(s) 136, 478, 572, 763, 840, 1545
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 2 cut(s) 127, 1088
BseMII CTCAG 5 cut(s) 52, 305, 411, 1170, 1481
BseRI GAGGAG 2 cut(s) 200, 782
BseXI GCAGC 5 cut(s) 64, 344, 542, 738, 1499
BshFI GGCC 3 cut(s) 120, 953, 1239
BshVI ATCGAT 1 cut(s) 1050
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 4 cut(s) 128, 192, 470, 1682
BslFI GGGAC 3 cut(s) 208, 1104, 1602
BslI CCNNNNNNNGG 2 cut(s) 127, 1088
BsmAI GTCTC 2 cut(s) 1144, 1497
BsmFI GGGAC 3 cut(s) 208, 1104, 1602
BsnI GGCC 3 cut(s) 120, 953, 1239
BsoBI CYCGRG 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 4 cut(s) 387, 967, 1051, 1678
Bsp19I CCATGG 1 cut(s) 990
BspANI GGCC 3 cut(s) 120, 953, 1239
BspCNI CTCAG 5 cut(s) 51, 304, 412, 1171, 1482
BspDI ATCGAT 1 cut(s) 1050
BspHI TCATGA 2 cut(s) 22, 1709
BspLI GGNNCC 3 cut(s) 189, 196, 988
BspMAI CTGCAG 2 cut(s) 334, 1627
BspPI GGATC 1 cut(s) 1673
BssECI CCNNGG 4 cut(s) 191, 990, 1549, 1665
BssMI GATC 4 cut(s) 387, 967, 1051, 1678
BssNI GRCGYC 1 cut(s) 262
BssT1I CCWWGG 2 cut(s) 990, 1665
Bst2UI CCWGG 2 cut(s) 809, 1206
Bst4CI ACNGT 4 cut(s) 62, 608, 923, 929
Bst6I CTCTTC 2 cut(s) 552, 653
BstACI GRCGYC 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 572
BstDEI CTNAG 7 cut(s) 38, 291, 420, 1085, 1179, 1280, 1490
BstDSI CCRYGG 1 cut(s) 990
BstF5I GGATG 6 cut(s) 136, 478, 572, 763, 840, 1545
BstKTI GATC 4 cut(s) 390, 970, 1054, 1681
BstMAI GTCTC 2 cut(s) 1144, 1497
BstMBI GATC 4 cut(s) 387, 967, 1051, 1678
BstMWI GCNNNNNNNGC 3 cut(s) 259, 268, 726
BstNI CCWGG 2 cut(s) 809, 1206
BstSCI CCNGG 4 cut(s) 190, 191, 807, 1204
BstSFI CTRYAG 2 cut(s) 330, 1623
BstV1I GCAGC 5 cut(s) 64, 344, 542, 738, 1499
BstV2I GAAGAC 2 cut(s) 1375, 1389
BstX2I RGATCY 1 cut(s) 1678
BstXI CCANNNNNNTGG 2 cut(s) 856, 1527
BstYI RGATCY 1 cut(s) 1678
Bsu15I ATCGAT 1 cut(s) 1050
BsuI GTATCC 1 cut(s) 613
BsuRI GGCC 3 cut(s) 120, 953, 1239
BsuTUI ATCGAT 1 cut(s) 1050
BtgI CCRYGG 1 cut(s) 990
BtsCI GGATG 6 cut(s) 136, 478, 572, 763, 840, 1545
BtsIMutI CAGTG 1 cut(s) 808
Cac8I GCNNGC 1 cut(s) 572
CciI TCATGA 2 cut(s) 22, 1709
Cfr13I GGNCC 2 cut(s) 195, 1530
Cfr9I CCCGGG 1 cut(s) 191
ClaI ATCGAT 1 cut(s) 1050
CseI GACGC 1 cut(s) 251
Csp6I GTAC 3 cut(s) 33, 428, 1011
CviAII CATG 6 cut(s) 23, 511, 991, 1445, 1578, 1710
CviQI GTAC 3 cut(s) 33, 428, 1011
DdeI CTNAG 7 cut(s) 38, 291, 420, 1085, 1179, 1280, 1490
DpnI GATC 4 cut(s) 389, 969, 1053, 1680
DpnII GATC 4 cut(s) 387, 967, 1051, 1678
Eam1104I CTCTTC 2 cut(s) 552, 653
EarI CTCTTC 2 cut(s) 552, 653
Eco130I CCWWGG 2 cut(s) 990, 1665
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 2 cut(s) 195, 1530
Eco57I CTGAAG 3 cut(s) 155, 784, 1494
Eco88I CYCGRG 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 195
EcoRII CCWGG 2 cut(s) 807, 1204
EcoT14I CCWWGG 2 cut(s) 990, 1665
EcoT22I ATGCAT 1 cut(s) 1633
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 2 cut(s) 990, 1665
FaeI CATG 6 cut(s) 26, 514, 994, 1448, 1581, 1713
FaqI GGGAC 3 cut(s) 208, 1104, 1602
FatI CATG 6 cut(s) 22, 510, 990, 1444, 1577, 1709
FbaI TGATCA 1 cut(s) 387
Fnu4HI GCNGC 5 cut(s) 78, 333, 531, 752, 1513
FokI GGATG 6 cut(s) 143, 485, 579, 770, 847, 1532
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 5 cut(s) 78, 333, 531, 752, 1513
FspBI CTAG 4 cut(s) 180, 626, 725, 935
GluI GCNGC 5 cut(s) 78, 333, 531, 752, 1513
GsuI CTGGAG 2 cut(s) 663, 1503
HaeIII GGCC 3 cut(s) 120, 953, 1239
HapII CCGG 4 cut(s) 128, 192, 470, 1682
HgaI GACGC 1 cut(s) 251
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 6 cut(s) 26, 514, 994, 1448, 1581, 1713
HincII GTYRAC 3 cut(s) 305, 359, 1199
HindII GTYRAC 3 cut(s) 305, 359, 1199
HindIII AAGCTT 1 cut(s) 1353
HinfI GANTC 8 cut(s) 15, 301, 714, 787, 871, 1367, 1441, 1706
HpaII CCGG 4 cut(s) 128, 192, 470, 1682
HphI GGTGA 3 cut(s) 127, 361, 787
Hpy166II GTNNAC 6 cut(s) 89, 305, 359, 480, 1199, 1324
Hpy188I TCNGA 9 cut(s) 41, 174, 294, 387, 786, 972, 1005, 1180, 1550
Hpy188III TCNNGA 7 cut(s) 23, 680, 1100, 1401, 1520, 1598, 1710
Hpy8I GTNNAC 6 cut(s) 89, 305, 359, 480, 1199, 1324
HpyAV CCTTC 9 cut(s) 131, 151, 229, 282, 385, 808, 824, 1286, 1334
HpyCH4III ACNGT 4 cut(s) 62, 608, 923, 929
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 8 cut(s) 332, 510, 720, 1186, 1350, 1373, 1625, 1631
HpyF10VI GCNNNNNNNGC 3 cut(s) 259, 268, 726
HpyF3I CTNAG 7 cut(s) 38, 291, 420, 1085, 1179, 1280, 1490
HpySE526I ACGT 1 cut(s) 684
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 6 cut(s) 26, 514, 994, 1448, 1581, 1713
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 4 cut(s) 387, 967, 1051, 1678
LmnI GCTCC 3 cut(s) 187, 682, 992
Lsp1109I GCAGC 5 cut(s) 64, 344, 542, 738, 1499
LweI GCATC 5 cut(s) 16, 557, 1173, 1618, 1640
MaeI CTAG 4 cut(s) 180, 626, 725, 935
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 4 cut(s) 133, 367, 688, 923
MalI GATC 4 cut(s) 389, 969, 1053, 1680
MboI GATC 4 cut(s) 387, 967, 1051, 1678
MfeI CAATTG 2 cut(s) 863, 1269
MflI RGATCY 1 cut(s) 1678
MhlI GDGCHC 1 cut(s) 192
MlyI GAGTC 3 cut(s) 310, 723, 1361
MmeI TCCRAC 2 cut(s) 1284, 1508
Mph1103I ATGCAT 1 cut(s) 1633
MroXI GAANNNNTTC 3 cut(s) 239, 252, 647
MseI TTAA 5 cut(s) 591, 704, 996, 1023, 1469
MslI CAYNNNNRTG 1 cut(s) 656
MspI CCGG 4 cut(s) 128, 192, 470, 1682
MspR9I CCNGG 4 cut(s) 192, 193, 809, 1206
MunI CAATTG 2 cut(s) 863, 1269
MvaI CCWGG 2 cut(s) 809, 1206
MwoI GCNNNNNNNGC 3 cut(s) 259, 268, 726
NciI CCSGG 2 cut(s) 192, 193
NcoI CCATGG 1 cut(s) 990
NdeII GATC 4 cut(s) 387, 967, 1051, 1678
NlaIII CATG 6 cut(s) 26, 514, 994, 1448, 1581, 1713
NlaIV GGNNCC 3 cut(s) 189, 196, 988
NmeAIII GCCGAG 1 cut(s) 96
NmuCI GTSAC 2 cut(s) 133, 367
NsiI ATGCAT 1 cut(s) 1633
PagI TCATGA 2 cut(s) 22, 1709
PdmI GAANNNNTTC 3 cut(s) 239, 252, 647
PfeI GAWTC 5 cut(s) 15, 787, 871, 1441, 1706
PkrI GCNGC 5 cut(s) 79, 334, 532, 753, 1514
PleI GAGTC 3 cut(s) 309, 722, 1361
PpsI GAGTC 3 cut(s) 309, 722, 1361
PpuMI RGGWCCY 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 195
Psp6I CCWGG 2 cut(s) 807, 1204
PspGI CCWGG 2 cut(s) 807, 1204
PspN4I GGNNCC 3 cut(s) 189, 196, 988
PspPI GGNCC 2 cut(s) 195, 1530
PspPPI RGGWCCY 1 cut(s) 195
PstI CTGCAG 2 cut(s) 334, 1627
PsuI RGATCY 1 cut(s) 1678
RsaI GTAC 3 cut(s) 34, 429, 1012
RsaNI GTAC 3 cut(s) 33, 428, 1011
RseI CAYNNNNRTG 1 cut(s) 656
SaqAI TTAA 5 cut(s) 591, 704, 996, 1023, 1469
SatI GCNGC 5 cut(s) 78, 333, 531, 752, 1513
Sau3AI GATC 4 cut(s) 387, 967, 1051, 1678
Sau96I GGNCC 2 cut(s) 195, 1530
SchI GAGTC 3 cut(s) 310, 723, 1361
ScrFI CCNGG 4 cut(s) 192, 193, 809, 1206
SduI GDGCHC 1 cut(s) 192
SfaNI GCATC 5 cut(s) 16, 557, 1173, 1618, 1640
SfcI CTRYAG 2 cut(s) 330, 1623
SinI GGWCC 2 cut(s) 195, 1530
SmaI CCCGGG 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 656
SpeI ACTAGT 1 cut(s) 625
SspI AATATT 2 cut(s) 1315, 1330
SspMI CTAG 4 cut(s) 180, 626, 725, 935
StyD4I CCNGG 4 cut(s) 190, 191, 807, 1204
StyI CCWWGG 2 cut(s) 990, 1665
TaaI ACNGT 4 cut(s) 62, 608, 923, 929
TaiI ACGT 1 cut(s) 687
TaqI TCGA 4 cut(s) 243, 603, 966, 1050
TaqII GACCGA 1 cut(s) 8
TatI WGTACW 2 cut(s) 32, 427
TfiI GAWTC 5 cut(s) 15, 787, 871, 1441, 1706
Tru1I TTAA 5 cut(s) 591, 704, 996, 1023, 1469
Tru9I TTAA 5 cut(s) 591, 704, 996, 1023, 1469
TscAI CASTG 1 cut(s) 808
TseFI GTSAC 2 cut(s) 133, 367
TseI GCWGC 5 cut(s) 77, 332, 530, 751, 1512
Tsp45I GTSAC 2 cut(s) 133, 367
TspDTI ATGAA 6 cut(s) 261, 648, 774, 1433, 1584, 1698
TspGWI ACGGA 3 cut(s) 272, 812, 1041
TspMI CCCGGG 1 cut(s) 191
TspRI CASTG 1 cut(s) 808
VpaK11BI GGWCC 2 cut(s) 195, 1530
XmaI CCCGGG 1 cut(s) 191
XmnI GAANNNNTTC 3 cut(s) 239, 252, 647
XspI CTAG 4 cut(s) 180, 626, 725, 935
Zsp2I ATGCAT 1 cut(s) 1633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.