RchiOBHm_Chr6g0267551

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015735
Physical Location & Seq
Forward (+)
24414651 .. 24417537
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23998

Sequence Viewer

Length: 735 bp
ATGCCGGCCAAGGCCTCCGAGCAGGAGAAGAATAACCTTGAGCTCTTCTTGAAGGTCGACGGACTGGATGAGCGGACGGCCAAACACACAATCGCCAACAACAACGTCACCGCCGTCATTCACGAGTTACTCATGGATGCAGTCGAAGCATTGGTTGCTAAAAAGTTTCCAGCAAATGCCCTTGTGCATCGCCCCGTATTGGCACACTACATTGTGTCTTCAAAGTTTCAGAGGAAGAAATCAAGCAATCTGTTAAATGAGGTTTTTGAAGAGAATAAGTCTGCGATTTTGGAGCAGCGCTATAAAACAAATGCGGGTGACTTATTTGCACATCTTAGGAAGAGACATCCGTGGGCTGACCCAGAAGTTCCAGTTGAAGAAATTCTTAATCCATATGCGATAAGCCCTCAACCAGAGGAAAACTTTAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCTATCTGAGGTTTGATGACACAAATCACGAAGCTGAAAAGAAGGAATATATTGATCATATTCAGGAAATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTACATTGATCATCAGACGGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGGCCAATTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

244

Amino Acids

28.47

Weight (kDa)

5.96

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_synt_1c_R1 PF04558 83 - 121 2.8e-06 Glutaminyl-tRNA synthetase, non-specific RNA binding region part 1
tRNA-synt_1c PF00749 143 - 243 1.8e-34 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 73
AccI GTMKAC 1 cut(s) 57
AciI CCGC 3 cut(s) 73, 111, 314
AcoI YGGCCR 2 cut(s) 6, 78
AcsI RAATTY 1 cut(s) 381
AfaI GTAC 1 cut(s) 686
AfeI AGCGCT 1 cut(s) 299
AfiI CCNNNNNNNGG 2 cut(s) 199, 715
AgsI TTSAA 4 cut(s) 52, 222, 269, 377
AhlI ACTAGT 1 cut(s) 587
AjnI CCWGG 1 cut(s) 450
AluBI AGCT 3 cut(s) 43, 509, 628
AluI AGCT 3 cut(s) 43, 509, 628
Alw21I GWGCWC 2 cut(s) 45, 630
Alw26I GTCTC 1 cut(s) 337
Aor51HI AGCGCT 1 cut(s) 299
AoxI GGCC 5 cut(s) 6, 12, 78, 448, 722
ApeKI GCWGC 2 cut(s) 295, 474
ApoI RAATTY 1 cut(s) 381
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 1 cut(s) 300
AsuHPI GGTGA 2 cut(s) 100, 329
BanII GRGCYC 3 cut(s) 45, 570, 630
BauI CACGAG 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 2 cut(s) 45, 630
BbvI GCAGC 2 cut(s) 307, 461
BccI CCATC 1 cut(s) 550
BceAI ACGGC 2 cut(s) 93, 98
BciT130I CCWGG 1 cut(s) 452
BclI TGATCA 2 cut(s) 529, 655
BcoDI GTCTC 1 cut(s) 337
BcuI ACTAGT 1 cut(s) 587
BfaI CTAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 301
BglII AGATCT 1 cut(s) 604
BisI GCNGC 2 cut(s) 296, 475
BlsI GCNGC 2 cut(s) 297, 476
Bme1390I CCNGG 1 cut(s) 452
BmiI GGNNCC 1 cut(s) 567
BmrFI CCNGG 1 cut(s) 452
BmsI GCATC 2 cut(s) 127, 196
BpiI GAAGAC 1 cut(s) 210
BpuEI CTTGAG 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 9, 350, 710
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 715
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 2 cut(s) 69, 371
Bse3DI GCAATG 1 cut(s) 438
BseBI CCWGG 1 cut(s) 452
BseDI CCNNGG 3 cut(s) 9, 350, 710
BseGI GGATG 5 cut(s) 73, 142, 346, 561, 676
BseLI CCNNNNNNNGG 2 cut(s) 199, 715
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 473
BseNI ACTGG 2 cut(s) 69, 371
BseXI GCAGC 2 cut(s) 307, 461
BshFI GGCC 5 cut(s) 8, 14, 80, 450, 724
BsiHKAI GWGCWC 2 cut(s) 45, 630
BsiSI CCGG 1 cut(s) 5
BslFI GGGAC 1 cut(s) 731
BslI CCNNNNNNNGG 2 cut(s) 199, 715
BsmAI GTCTC 1 cut(s) 337
BsmFI GGGAC 1 cut(s) 731
BsnI GGCC 5 cut(s) 8, 14, 80, 450, 724
Bsp1286I GDGCHC 3 cut(s) 45, 570, 630
Bsp143I GATC 4 cut(s) 529, 576, 604, 655
BspACI CCGC 3 cut(s) 73, 111, 314
BspANI GGCC 5 cut(s) 8, 14, 80, 450, 724
BspCNI CTCAG 1 cut(s) 474
BspLI GGNNCC 1 cut(s) 567
BspQI GCTCTTC 1 cut(s) 50
BsrBI CCGCTC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 438
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 2 cut(s) 69, 371
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 3 cut(s) 9, 350, 710
BssMI GATC 4 cut(s) 529, 576, 604, 655
BssSI CACGAG 1 cut(s) 122
BssT1I CCWWGG 2 cut(s) 9, 710
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 1 cut(s) 452
Bst6I CTCTTC 3 cut(s) 50, 264, 335
BstAPI GCANNNNNTGC 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 335, 482
BstDSI CCRYGG 1 cut(s) 350
BstF5I GGATG 5 cut(s) 73, 142, 346, 561, 676
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 4 cut(s) 532, 579, 607, 658
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 4 cut(s) 529, 576, 604, 655
BstMWI GCNNNNNNNGC 2 cut(s) 146, 155
BstNI CCWGG 1 cut(s) 452
BstSCI CCNGG 1 cut(s) 450
BstV1I GCAGC 2 cut(s) 307, 461
BstV2I GAAGAC 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BsuRI GGCC 5 cut(s) 8, 14, 80, 450, 724
BtgI CCRYGG 1 cut(s) 350
BtgZI GCGATG 1 cut(s) 173
BtsCI GGATG 5 cut(s) 73, 142, 346, 561, 676
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 1 cut(s) 627
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 300
Cfr10I RCCGGY 1 cut(s) 4
Csp6I GTAC 1 cut(s) 685
CviAII CATG 1 cut(s) 133
CviQI GTAC 1 cut(s) 685
DdeI CTNAG 2 cut(s) 335, 482
DpnI GATC 4 cut(s) 531, 578, 606, 657
DpnII GATC 4 cut(s) 529, 576, 604, 655
EaeI YGGCCR 2 cut(s) 6, 78
Eam1104I CTCTTC 3 cut(s) 50, 264, 335
EarI CTCTTC 3 cut(s) 50, 264, 335
Ecl136II GAGCTC 2 cut(s) 43, 628
Eco130I CCWWGG 2 cut(s) 9, 710
Eco147I AGGCCT 1 cut(s) 14
Eco24I GRGCYC 3 cut(s) 45, 570, 630
Eco47III AGCGCT 1 cut(s) 299
Eco53kI GAGCTC 2 cut(s) 43, 628
EcoICRI GAGCTC 2 cut(s) 43, 628
EcoRII CCWGG 1 cut(s) 450
EcoT14I CCWWGG 2 cut(s) 9, 710
EcoT38I GRGCYC 3 cut(s) 45, 570, 630
ErhI CCWWGG 2 cut(s) 9, 710
FaeI CATG 1 cut(s) 136
FaiI YATR 8 cut(s) 134, 303, 394, 396, 525, 534, 612, 632
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FaqI GGGAC 1 cut(s) 731
FatI CATG 1 cut(s) 132
FauI CCCGC 1 cut(s) 307
FauNDI CATATG 1 cut(s) 394
FbaI TGATCA 2 cut(s) 529, 655
FblI GTMKAC 1 cut(s) 57
Fnu4HI GCNGC 2 cut(s) 296, 475
FokI GGATG 5 cut(s) 80, 149, 333, 568, 683
FriOI GRGCYC 3 cut(s) 45, 570, 630
Fsp4HI GCNGC 2 cut(s) 296, 475
FspBI CTAG 1 cut(s) 588
GlaI GCGC 1 cut(s) 299
GluI GCNGC 2 cut(s) 296, 475
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 5 cut(s) 8, 14, 80, 450, 724
HapII CCGG 1 cut(s) 5
HhaI GCGC 1 cut(s) 300
Hin1II CATG 1 cut(s) 136
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HpaII CCGG 1 cut(s) 5
HphI GGTGA 2 cut(s) 100, 329
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 4 cut(s) 19, 231, 483, 663
Hpy188III TCNNGA 4 cut(s) 49, 122, 503, 539
Hpy8I GTNNAC 1 cut(s) 58
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 3 cut(s) 46, 511, 630
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 3 cut(s) 140, 187, 329
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 155
HpyF3I CTNAG 2 cut(s) 335, 482
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 1 cut(s) 136
HspAI GCGC 1 cut(s) 298
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 529, 655
Kzo9I GATC 4 cut(s) 529, 576, 604, 655
LguI GCTCTTC 1 cut(s) 50
LmnI GCTCC 3 cut(s) 292, 565, 625
Lsp1109I GCAGC 2 cut(s) 307, 461
LweI GCATC 2 cut(s) 127, 196
MaeI CTAG 1 cut(s) 588
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 4 cut(s) 106, 126, 317, 641
MalI GATC 4 cut(s) 531, 578, 606, 657
MbiI CCGCTC 1 cut(s) 73
MboI GATC 4 cut(s) 529, 576, 604, 655
MboII GAAGA 7 cut(s) 37, 40, 210, 247, 281, 352, 389
MfeI CAATTG 1 cut(s) 550
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 3 cut(s) 45, 570, 630
MluCI AATT 5 cut(s) 381, 543, 550, 614, 726
MnlI CCTC 8 cut(s) 25, 225, 253, 409, 417, 458, 477, 708
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 4 cut(s) 254, 387, 426, 729
MspI CCGG 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 452
MunI CAATTG 1 cut(s) 550
MvaI CCWGG 1 cut(s) 452
MwoI GCNNNNNNNGC 2 cut(s) 146, 155
NaeI GCCGGC 1 cut(s) 6
NdeI CATATG 1 cut(s) 394
NdeII GATC 4 cut(s) 529, 576, 604, 655
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 1 cut(s) 567
NmuCI GTSAC 3 cut(s) 106, 317, 641
PceI AGGCCT 1 cut(s) 14
PciSI GCTCTTC 1 cut(s) 50
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 381
PkrI GCNGC 2 cut(s) 297, 476
Psp124BI GAGCTC 2 cut(s) 45, 630
Psp6I CCWGG 1 cut(s) 450
PspGI CCWGG 1 cut(s) 450
PspN4I GGNNCC 1 cut(s) 567
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 1 cut(s) 686
RsaNI GTAC 1 cut(s) 685
SacI GAGCTC 2 cut(s) 45, 630
SalI GTCGAC 1 cut(s) 56
SapI GCTCTTC 1 cut(s) 50
SaqAI TTAA 4 cut(s) 254, 387, 426, 729
SatI GCNGC 2 cut(s) 296, 475
Sau3AI GATC 4 cut(s) 529, 576, 604, 655
ScrFI CCNGG 1 cut(s) 452
SduI GDGCHC 3 cut(s) 45, 570, 630
SetI ASST 9 cut(s) 39, 45, 57, 108, 264, 469, 488, 511, 630
SfaNI GCATC 2 cut(s) 127, 196
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
SpeI ACTAGT 1 cut(s) 587
Sse9I AATT 5 cut(s) 381, 543, 550, 614, 726
SseBI AGGCCT 1 cut(s) 14
SsiI CCGC 3 cut(s) 73, 111, 314
SspMI CTAG 1 cut(s) 588
SstI GAGCTC 2 cut(s) 45, 630
StuI AGGCCT 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 450
StyI CCWWGG 2 cut(s) 9, 710
TaiI ACGT 1 cut(s) 108
TaqI TCGA 2 cut(s) 57, 144
TasI AATT 5 cut(s) 381, 543, 550, 614, 726
TatI WGTACW 1 cut(s) 684
Tru1I TTAA 4 cut(s) 254, 387, 426, 729
Tru9I TTAA 4 cut(s) 254, 387, 426, 729
TscAI CASTG 1 cut(s) 627
TseFI GTSAC 3 cut(s) 106, 317, 641
TseI GCWGC 2 cut(s) 295, 474
Tsp45I GTSAC 3 cut(s) 106, 317, 641
TspDTI ATGAA 2 cut(s) 627, 716
TspGWI ACGGA 2 cut(s) 75, 339
TspRI CASTG 1 cut(s) 627
XapI RAATTY 1 cut(s) 381
XmiI GTMKAC 1 cut(s) 57
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.