Rh6BG156300

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015735
Physical Location & Seq
Forward (+)
25246875 .. 25248370
1496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG156300.1

Sequence Viewer

Length: 486 bp
ATGAAAGAGAAGCCTGCTAAACTAGAGGAAATTGCTATTGTTGTTTCTACCCTGAATAGCCAGTTGAAGAAATTCTTAATCCCTATGCAATATTCCCTCAACCAGAGGAAAACTTTAACGATCATACTTCAGTTTTCTTTAGTGATGCTGAATGGATATATACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCTATCTGAGGTTTGATGACACAAATCACGAAGCTGAAAAGAAGGAATATATTGATCATATTCAGGAAATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGGGGTCACGCTTACGTTGATCATCAGACGGGGGATGAGATAAAGGAGTACAGGGAGAAAAAAATGAACAGCCCTCGGAGGGACAGGCCAATTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

161

Amino Acids

19.05

Weight (kDa)

7.82

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c PF00749 51 - 160 2.8e-42 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 113
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 2 cut(s) 178, 466
AgsI TTSAA 1 cut(s) 67
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 2 cut(s) 260, 379
AluI AGCT 2 cut(s) 260, 379
Alw21I GWGCWC 1 cut(s) 381
AoxI GGCC 3 cut(s) 169, 199, 473
ApeKI GCWGC 1 cut(s) 225
ApoI RAATTY 1 cut(s) 71
Asp700I GAANNNNTTC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 321, 381
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 1 cut(s) 212
BccI CCATC 1 cut(s) 301
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 2 cut(s) 280, 406
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 2 cut(s) 23, 339
BglII AGATCT 1 cut(s) 355
BisI GCNGC 1 cut(s) 226
BlsI GCNGC 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 177, 461
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 466
Bse1I ACTGG 1 cut(s) 61
Bse3DI GCAATG 1 cut(s) 189
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 177, 461
BseGI GGATG 2 cut(s) 312, 427
BseLI CCNNNNNNNGG 2 cut(s) 178, 466
BseMI GCAATG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 1 cut(s) 61
BseXI GCAGC 1 cut(s) 212
BshFI GGCC 3 cut(s) 171, 201, 475
BsiHKAI GWGCWC 1 cut(s) 381
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 2 cut(s) 178, 466
BsmFI GGGAC 1 cut(s) 482
BsnI GGCC 3 cut(s) 171, 201, 475
Bsp1286I GDGCHC 2 cut(s) 321, 381
Bsp143I GATC 5 cut(s) 120, 280, 327, 355, 406
BspANI GGCC 3 cut(s) 171, 201, 475
BspCNI CTCAG 1 cut(s) 225
BspLI GGNNCC 1 cut(s) 318
BsrDI GCAATG 1 cut(s) 189
BsrI ACTGG 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 177, 461
BssMI GATC 5 cut(s) 120, 280, 327, 355, 406
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 203
BstC8I GCNNGC 1 cut(s) 15
BstDEI CTNAG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 312, 427
BstKTI GATC 5 cut(s) 123, 283, 330, 358, 409
BstMBI GATC 5 cut(s) 120, 280, 327, 355, 406
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 212
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BsuRI GGCC 3 cut(s) 171, 201, 475
BtsCI GGATG 2 cut(s) 312, 427
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 15
Csp6I GTAC 1 cut(s) 436
CviAII CATG 1 cut(s) 173
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 1 cut(s) 233
DpnI GATC 5 cut(s) 122, 282, 329, 357, 408
DpnII GATC 5 cut(s) 120, 280, 327, 355, 406
Ecl136II GAGCTC 1 cut(s) 379
Eco130I CCWWGG 1 cut(s) 177
Eco24I GRGCYC 2 cut(s) 321, 381
Eco53kI GAGCTC 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 177
EcoT38I GRGCYC 2 cut(s) 321, 381
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 1 cut(s) 176
FalI AAGNNNNNCTT 2 cut(s) 59, 91
FaqI GGGAC 1 cut(s) 482
FatI CATG 1 cut(s) 172
FbaI TGATCA 2 cut(s) 280, 406
Fnu4HI GCNGC 1 cut(s) 226
FokI GGATG 2 cut(s) 319, 434
FriOI GRGCYC 2 cut(s) 321, 381
Fsp4HI GCNGC 1 cut(s) 226
FspBI CTAG 2 cut(s) 23, 339
GluI GCNGC 1 cut(s) 226
HaeIII GGCC 3 cut(s) 171, 201, 475
Hin1II CATG 1 cut(s) 176
Hpy188I TCNGA 3 cut(s) 234, 414, 465
Hpy188III TCNNGA 2 cut(s) 254, 290
HpyAV CCTTC 2 cut(s) 262, 381
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 1 cut(s) 88
HpyF3I CTNAG 1 cut(s) 233
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 1 cut(s) 176
Ksp22I TGATCA 2 cut(s) 280, 406
Kzo9I GATC 5 cut(s) 120, 280, 327, 355, 406
LmnI GCTCC 2 cut(s) 316, 376
LpnPI CCDG 9 cut(s) 27, 65, 74, 116, 188, 215, 275, 424, 457
Lsp1109I GCAGC 1 cut(s) 212
LweI GCATC 1 cut(s) 135
MaeI CTAG 2 cut(s) 23, 339
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 392
MalI GATC 5 cut(s) 122, 282, 329, 357, 408
MboI GATC 5 cut(s) 120, 280, 327, 355, 406
MboII GAAGA 1 cut(s) 79
MfeI CAATTG 1 cut(s) 301
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 2 cut(s) 321, 381
MluCI AATT 6 cut(s) 30, 71, 294, 301, 365, 477
MnlI CCTC 7 cut(s) 19, 99, 107, 209, 228, 459, 471
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 3 cut(s) 77, 116, 480
MspR9I CCNGG 1 cut(s) 203
MunI CAATTG 1 cut(s) 301
MvaI CCWGG 1 cut(s) 203
NdeII GATC 5 cut(s) 120, 280, 327, 355, 406
NlaIII CATG 1 cut(s) 176
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 1 cut(s) 392
PdmI GAANNNNTTC 1 cut(s) 71
PkrI GCNGC 1 cut(s) 227
Psp124BI GAGCTC 1 cut(s) 381
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 318
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
SacI GAGCTC 1 cut(s) 381
SaqAI TTAA 3 cut(s) 77, 116, 480
SatI GCNGC 1 cut(s) 226
Sau3AI GATC 5 cut(s) 120, 280, 327, 355, 406
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 2 cut(s) 321, 381
SetI ASST 5 cut(s) 220, 239, 262, 381, 405
SfaNI GCATC 1 cut(s) 135
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 6 cut(s) 30, 71, 294, 301, 365, 477
SspI AATATT 1 cut(s) 92
SspMI CTAG 2 cut(s) 23, 339
SstI GAGCTC 1 cut(s) 381
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 177
TaiI ACGT 1 cut(s) 405
TasI AATT 6 cut(s) 30, 71, 294, 301, 365, 477
TatI WGTACW 1 cut(s) 435
Tru1I TTAA 3 cut(s) 77, 116, 480
Tru9I TTAA 3 cut(s) 77, 116, 480
TscAI CASTG 1 cut(s) 378
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 1 cut(s) 225
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 3 cut(s) 17, 378, 467
TspRI CASTG 1 cut(s) 378
XapI RAATTY 1 cut(s) 71
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 2 cut(s) 23, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.