Rw6G036060

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015735
Physical Location & Seq
Reverse (-)
60635500 .. 60636838
1339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G036060.1

Sequence Viewer

Length: 474 bp
ATGTGGAAGCCTGCAAAACTAGAGGAAATTGCTATTGCTGTTTCTACCCTGAATAGCCAGTTGAAGAAATTCTTAATCCATATGCGATATTCCCTCAACCAGAGGAAAACTTTAAGATGTTGCAACTCAAAGGAACTCCTTGAAAAACACTTGAGTGTGACACGCTTTCCTCCTGAGCTGAATGGATATATACATAAAAGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGGGGTGGTGGCTGCTATCTGAGGTTTGATGACACAAATCACGAAGCTGAAAAGAAGGAATATATTTATCATATTCAGGAAATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAACGGGTCACGCTTACGTTGATCATCAGGGAATTTCTGTCACTACAATGGGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

157

Amino Acids

18.41

Weight (kDa)

7.75

Isoelectric Point (pI)

24.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c PF00749 53 - 148 3e-36 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 68, 447
AfiI CCNNNNNNNGG 1 cut(s) 208
AgsI TTSAA 2 cut(s) 64, 143
AhlI ACTAGT 1 cut(s) 368
AjnI CCWGG 1 cut(s) 231
AleI CACNNNNGTG 1 cut(s) 153
AluBI AGCT 3 cut(s) 178, 290, 409
AluI AGCT 3 cut(s) 178, 290, 409
Alw21I GWGCWC 1 cut(s) 411
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 1 cut(s) 255
ApoI RAATTY 2 cut(s) 68, 447
Asp700I GAANNNNTTC 1 cut(s) 68
BanII GRGCYC 2 cut(s) 351, 411
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 1 cut(s) 242
BccI CCATC 1 cut(s) 331
BciT130I CCWGG 1 cut(s) 233
BclI TGATCA 1 cut(s) 436
BcuI ACTAGT 1 cut(s) 368
BfaI CTAG 2 cut(s) 20, 369
BglII AGATCT 1 cut(s) 385
BisI GCNGC 1 cut(s) 256
BlsI GCNGC 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 233
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 1 cut(s) 233
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 207
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 219
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 1 cut(s) 207
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 2 cut(s) 165, 254
BseNI ACTGG 1 cut(s) 58
BseXI GCAGC 1 cut(s) 242
BshFI GGCC 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 411
BslI CCNNNNNNNGG 1 cut(s) 208
BsnI GGCC 1 cut(s) 231
Bsp1286I GDGCHC 2 cut(s) 351, 411
Bsp143I GATC 3 cut(s) 357, 385, 436
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 2 cut(s) 166, 255
BspLI GGNNCC 1 cut(s) 348
BsrDI GCAATG 1 cut(s) 219
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 3 cut(s) 357, 385, 436
BssT1I CCWWGG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 2 cut(s) 174, 263
BstF5I GGATG 1 cut(s) 342
BstKTI GATC 3 cut(s) 360, 388, 439
BstMBI GATC 3 cut(s) 357, 385, 436
BstNI CCWGG 1 cut(s) 233
BstSCI CCNGG 1 cut(s) 231
BstV1I GCAGC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 1 cut(s) 231
BtsCI GGATG 1 cut(s) 342
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 1 cut(s) 408
Cac8I GCNNGC 1 cut(s) 12
CviAII CATG 1 cut(s) 203
CviJI RGCY 9 cut(s) 10, 57, 178, 201, 231, 255, 290, 349, 409
CviKI_1 RGCY 9 cut(s) 10, 57, 178, 201, 231, 255, 290, 349, 409
DdeI CTNAG 2 cut(s) 174, 263
DpnI GATC 3 cut(s) 359, 387, 438
DpnII GATC 3 cut(s) 357, 385, 436
Ecl136II GAGCTC 1 cut(s) 409
Eco130I CCWWGG 1 cut(s) 207
Eco24I GRGCYC 2 cut(s) 351, 411
Eco53kI GAGCTC 1 cut(s) 409
EcoICRI GAGCTC 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 231
EcoT14I CCWWGG 1 cut(s) 207
EcoT38I GRGCYC 2 cut(s) 351, 411
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 1 cut(s) 206
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FatI CATG 1 cut(s) 202
FauNDI CATATG 1 cut(s) 81
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 1 cut(s) 256
FokI GGATG 1 cut(s) 349
FriOI GRGCYC 2 cut(s) 351, 411
Fsp4HI GCNGC 1 cut(s) 256
FspBI CTAG 2 cut(s) 20, 369
GluI GCNGC 1 cut(s) 256
HaeIII GGCC 1 cut(s) 231
Hin1II CATG 1 cut(s) 206
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 3 cut(s) 173, 284, 320
HpyAV CCTTC 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 432
HpyCH4V TGCA 2 cut(s) 14, 123
HpyF3I CTNAG 2 cut(s) 174, 263
HpySE526I ACGT 1 cut(s) 432
Hsp92II CATG 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 3 cut(s) 357, 385, 436
LmnI GCTCC 2 cut(s) 346, 406
LpnPI CCDG 9 cut(s) 24, 62, 71, 113, 186, 218, 245, 305, 428
Lsp1109I GCAGC 1 cut(s) 242
MaeI CTAG 2 cut(s) 20, 369
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 3 cut(s) 157, 422, 454
MalI GATC 3 cut(s) 359, 387, 438
MboI GATC 3 cut(s) 357, 385, 436
MboII GAAGA 1 cut(s) 76
MfeI CAATTG 1 cut(s) 331
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 2 cut(s) 351, 411
MluCI AATT 6 cut(s) 27, 68, 324, 331, 395, 447
MnlI CCTC 6 cut(s) 16, 96, 104, 180, 237, 258
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 2 cut(s) 74, 113
MslI CAYNNNNRTG 2 cut(s) 153, 461
MspR9I CCNGG 1 cut(s) 233
MunI CAATTG 1 cut(s) 331
MvaI CCWGG 1 cut(s) 233
NdeI CATATG 1 cut(s) 81
NdeII GATC 3 cut(s) 357, 385, 436
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 1 cut(s) 348
NmuCI GTSAC 3 cut(s) 157, 422, 454
OliI CACNNNNGTG 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 68
PkrI GCNGC 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 1 cut(s) 348
PsuI RGATCY 1 cut(s) 385
RseI CAYNNNNRTG 2 cut(s) 153, 461
SacI GAGCTC 1 cut(s) 411
SaqAI TTAA 2 cut(s) 74, 113
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 3 cut(s) 357, 385, 436
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 2 cut(s) 351, 411
SetI ASST 5 cut(s) 180, 269, 292, 411, 435
SmiMI CAYNNNNRTG 2 cut(s) 153, 461
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
SpeI ACTAGT 1 cut(s) 368
Sse9I AATT 6 cut(s) 27, 68, 324, 331, 395, 447
SspMI CTAG 2 cut(s) 20, 369
SstI GAGCTC 1 cut(s) 411
StyD4I CCNGG 1 cut(s) 231
StyI CCWWGG 1 cut(s) 207
TaiI ACGT 1 cut(s) 435
TasI AATT 6 cut(s) 27, 68, 324, 331, 395, 447
Tru1I TTAA 2 cut(s) 74, 113
Tru9I TTAA 2 cut(s) 74, 113
TscAI CASTG 1 cut(s) 408
TseFI GTSAC 3 cut(s) 157, 422, 454
TseI GCWGC 1 cut(s) 255
Tsp45I GTSAC 3 cut(s) 157, 422, 454
TspDTI ATGAA 1 cut(s) 408
TspRI CASTG 1 cut(s) 408
XapI RAATTY 2 cut(s) 68, 447
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 2 cut(s) 20, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.