Rroxscaffold_4G00325430

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015735
Physical Location & Seq
Reverse (-)
57157933 .. 57161454
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325430.1

Sequence Viewer

Length: 294 bp
ATGAAAGCAGCTCTTGCTTCAGTGGTGGGGAGCAAAACCTTCGTGTTCAAGGTCGGGAGAGATTGTCAATTGGATGGGTTGGGAGCCCCACAAGATCACTTACACTACGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAAGAGGTCATGCTCACGTTGATTATCGGACGGGGGATGAGATAAAGGAGTACGGGGAGAAAAAAATGAACAGCCCTTGGAGGGACAGACCAATTAAAGAGTCGCTGAAGCTTTTTGAGGATATGAGGCGTGGCACTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

97

Amino Acids

11.26

Weight (kDa)

6.41

Isoelectric Point (pI)

32.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c PF00749 37 - 96 1.5e-12 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 1 cut(s) 49
AluBI AGCT 3 cut(s) 11, 145, 262
AluI AGCT 3 cut(s) 11, 145, 262
Alw21I GWGCWC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 8
BanII GRGCYC 2 cut(s) 88, 147
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 20
BccI CCATC 1 cut(s) 68
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BglII AGATCT 1 cut(s) 121
BisI GCNGC 1 cut(s) 9
BlsI GCNGC 1 cut(s) 10
BmiI GGNNCC 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 1 cut(s) 232
BseDI CCNNGG 1 cut(s) 227
BseGI GGATG 2 cut(s) 79, 193
BseLI CCNNNNNNNGG 1 cut(s) 232
BseXI GCAGC 1 cut(s) 20
BsiHKAI GWGCWC 1 cut(s) 147
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 248
Bsp1286I GDGCHC 2 cut(s) 88, 147
Bsp143I GATC 2 cut(s) 94, 121
BspLI GGNNCC 1 cut(s) 85
BssECI CCNNGG 1 cut(s) 227
BssMI GATC 2 cut(s) 94, 121
BssT1I CCWWGG 1 cut(s) 227
Bst6I CTCTTC 1 cut(s) 148
BstAPI GCANNNNNTGC 1 cut(s) 14
BstF5I GGATG 2 cut(s) 79, 193
BstKTI GATC 2 cut(s) 97, 124
BstMBI GATC 2 cut(s) 94, 121
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BtsCI GGATG 2 cut(s) 79, 193
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 3 cut(s) 27, 144, 285
Csp6I GTAC 1 cut(s) 202
CviAII CATG 1 cut(s) 161
CviJI RGCY 5 cut(s) 11, 86, 145, 225, 262
CviKI_1 RGCY 5 cut(s) 11, 86, 145, 225, 262
CviQI GTAC 1 cut(s) 202
DpnI GATC 2 cut(s) 96, 123
DpnII GATC 2 cut(s) 94, 121
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 145
Eco130I CCWWGG 1 cut(s) 227
Eco24I GRGCYC 2 cut(s) 88, 147
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 145
EcoT14I CCWWGG 1 cut(s) 227
EcoT38I GRGCYC 2 cut(s) 88, 147
ErhI CCWWGG 1 cut(s) 227
FaeI CATG 1 cut(s) 164
FaiI YATR 4 cut(s) 129, 149, 162, 275
FalI AAGNNNNNCTT 1 cut(s) 29
FaqI GGGAC 1 cut(s) 248
FatI CATG 1 cut(s) 160
Fnu4HI GCNGC 1 cut(s) 9
FokI GGATG 2 cut(s) 86, 200
FriOI GRGCYC 2 cut(s) 88, 147
Fsp4HI GCNGC 1 cut(s) 9
GluI GCNGC 1 cut(s) 9
Hin1II CATG 1 cut(s) 164
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 1 cut(s) 251
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 1 cut(s) 55
HpyAV CCTTC 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 168
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 1 cut(s) 164
Kzo9I GATC 2 cut(s) 94, 121
LmnI GCTCC 3 cut(s) 30, 83, 142
Lsp1109I GCAGC 1 cut(s) 20
MaeII ACGT 1 cut(s) 168
MalI GATC 2 cut(s) 96, 123
MboI GATC 2 cut(s) 94, 121
MboII GAAGA 1 cut(s) 165
MfeI CAATTG 1 cut(s) 68
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 2 cut(s) 88, 147
MluCI AATT 3 cut(s) 68, 131, 243
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 4 cut(s) 149, 225, 262, 270
MseI TTAA 1 cut(s) 246
MunI CAATTG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 14
NdeII GATC 2 cut(s) 94, 121
NlaIII CATG 1 cut(s) 164
NlaIV GGNNCC 1 cut(s) 85
PkrI GCNGC 1 cut(s) 10
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
Psp124BI GAGCTC 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 85
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SacI GAGCTC 1 cut(s) 147
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 1 cut(s) 9
Sau3AI GATC 2 cut(s) 94, 121
SchI GAGTC 1 cut(s) 260
SduI GDGCHC 2 cut(s) 88, 147
SetI ASST 7 cut(s) 13, 41, 54, 147, 160, 171, 264
Sse9I AATT 3 cut(s) 68, 131, 243
SstI GAGCTC 1 cut(s) 147
StyI CCWWGG 1 cut(s) 227
TaiI ACGT 1 cut(s) 171
TasI AATT 3 cut(s) 68, 131, 243
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 3 cut(s) 27, 144, 292
TseI GCWGC 1 cut(s) 8
TspDTI ATGAA 3 cut(s) 17, 144, 233
TspRI CASTG 3 cut(s) 27, 144, 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.