Rroxscaffold_5G00351520

Belongs to the class-I aminoacyl-tRNA synthetase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015735
Physical Location & Seq
Forward (+)
27717905 .. 27719100
1196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351520.1

Sequence Viewer

Length: 504 bp
ATGTGGAAGCCTGCAAAACTAGAGCCAGTTGAAGAAATTCTTAATCCATATGCGATATTCCCTCAACCAGAGGAAAACTTTAAGGTTCATACTTCAGTTTTCTTTAGTGATAGTTCTATTCTGAGATGTTGCAACTCAAAGGAACTCCTTGAAAAACACTTGAGTGTGACTCGCTTTCCTCCTGAGCTGAATGGATATATACATATAGGCCATGCCAAGGCAATGTTTATTGACTTTGGCCTGGCAAAAGAGAGAGGTGGTGGCTGCTATCCGAGGTTTGATGACACAAATCACGAAGCTGAAAAGAAGGAATATATTTATCATATTCAGGAAATTGTCAATTGGATGGGTTGGGAGCCCTACAAGATCACTTACACTAGTGATTACTTCCAAGATCTGTATGAATTAGCAGTGGAGCTCATACGAACGGGTCACGCTTACGTTGATCATCGGGTTTTGTTTTATAGTATTTCAAATACTTTGGAAATTGCTTTCCATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 ko01100 map00970 map01100
Pfam Domains
Protein Families
tRNA-synt_1c tRNA-synt_1c_C tRNA_synt_1c_R1 tRNA_synt_1c_R2

Protein Analysis

167

Amino Acids

19.6

Weight (kDa)

5.55

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1c PF00749 56 - 150 8.5e-38 tRNA synthetases class I (E and Q), catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 78
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 3 cut(s) 32, 152, 474
AhlI ACTAGT 1 cut(s) 377
AjnI CCWGG 1 cut(s) 240
AleI CACNNNNGTG 1 cut(s) 162
AluBI AGCT 3 cut(s) 187, 299, 418
AluI AGCT 3 cut(s) 187, 299, 418
Alw21I GWGCWC 1 cut(s) 420
AoxI GGCC 2 cut(s) 208, 238
ApeKI GCWGC 1 cut(s) 264
ApoI RAATTY 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 36
BanII GRGCYC 2 cut(s) 360, 420
Bbv12I GWGCWC 1 cut(s) 420
BbvI GCAGC 1 cut(s) 251
BccI CCATC 1 cut(s) 340
BciT130I CCWGG 1 cut(s) 242
BclI TGATCA 1 cut(s) 445
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 2 cut(s) 20, 378
BglII AGATCT 1 cut(s) 394
BisI GCNGC 1 cut(s) 265
BlsI GCNGC 1 cut(s) 266
Bme1390I CCNGG 1 cut(s) 242
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 1 cut(s) 242
BplI GAGNNNNNCTC 2 cut(s) 154, 186
Bpu10I CCTNAGC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 181
BsaJI CCNNGG 2 cut(s) 216, 272
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 1 cut(s) 26
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 2 cut(s) 216, 272
BseGI GGATG 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 2 cut(s) 113, 174
BseNI ACTGG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 251
BshFI GGCC 2 cut(s) 210, 240
BsiHKAI GWGCWC 1 cut(s) 420
BslI CCNNNNNNNGG 1 cut(s) 217
BsnI GGCC 2 cut(s) 210, 240
Bsp1286I GDGCHC 2 cut(s) 360, 420
Bsp143I GATC 3 cut(s) 366, 394, 445
BspANI GGCC 2 cut(s) 210, 240
BspCNI CTCAG 2 cut(s) 114, 175
BspLI GGNNCC 1 cut(s) 357
BsrDI GCAATG 1 cut(s) 228
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 2 cut(s) 216, 272
BssMI GATC 3 cut(s) 366, 394, 445
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 242
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 3 cut(s) 122, 183, 501
BstF5I GGATG 1 cut(s) 351
BstKTI GATC 3 cut(s) 369, 397, 448
BstMBI GATC 3 cut(s) 366, 394, 445
BstMWI GCNNNNNNNGC 1 cut(s) 497
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 1 cut(s) 240
BstV1I GCAGC 1 cut(s) 251
BstX2I RGATCY 1 cut(s) 394
BstYI RGATCY 1 cut(s) 394
BsuRI GGCC 2 cut(s) 210, 240
BtsCI GGATG 1 cut(s) 351
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 1 cut(s) 417
Cac8I GCNNGC 1 cut(s) 12
CviAII CATG 2 cut(s) 212, 497
CviJI RGCY 9 cut(s) 10, 25, 187, 210, 240, 264, 299, 358, 418
CviKI_1 RGCY 9 cut(s) 10, 25, 187, 210, 240, 264, 299, 358, 418
DdeI CTNAG 3 cut(s) 122, 183, 501
DpnI GATC 3 cut(s) 368, 396, 447
DpnII GATC 3 cut(s) 366, 394, 445
Ecl136II GAGCTC 1 cut(s) 418
Eco130I CCWWGG 1 cut(s) 216
Eco24I GRGCYC 2 cut(s) 360, 420
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 240
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 2 cut(s) 360, 420
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 2 cut(s) 215, 500
FalI AAGNNNNNCTT 2 cut(s) 24, 56
FatI CATG 2 cut(s) 211, 496
FauNDI CATATG 1 cut(s) 49
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 1 cut(s) 265
FokI GGATG 1 cut(s) 358
FriOI GRGCYC 2 cut(s) 360, 420
Fsp4HI GCNGC 1 cut(s) 265
FspBI CTAG 2 cut(s) 20, 378
GluI GCNGC 1 cut(s) 265
HaeIII GGCC 2 cut(s) 210, 240
Hin1II CATG 2 cut(s) 215, 500
HinfI GANTC 1 cut(s) 169
Hpy188I TCNGA 2 cut(s) 123, 273
Hpy188III TCNNGA 3 cut(s) 182, 293, 329
HpyAV CCTTC 1 cut(s) 301
HpyCH4IV ACGT 1 cut(s) 441
HpyCH4V TGCA 2 cut(s) 14, 132
HpyF10VI GCNNNNNNNGC 1 cut(s) 497
HpyF3I CTNAG 3 cut(s) 122, 183, 501
HpySE526I ACGT 1 cut(s) 441
Hsp92II CATG 2 cut(s) 215, 500
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 3 cut(s) 366, 394, 445
LmnI GCTCC 2 cut(s) 355, 415
LpnPI CCDG 7 cut(s) 24, 39, 81, 195, 227, 254, 314
Lsp1109I GCAGC 1 cut(s) 251
MaeI CTAG 2 cut(s) 20, 378
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 2 cut(s) 166, 431
MalI GATC 3 cut(s) 368, 396, 447
MboI GATC 3 cut(s) 366, 394, 445
MboII GAAGA 1 cut(s) 44
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 1 cut(s) 394
MhlI GDGCHC 2 cut(s) 360, 420
MluCI AATT 5 cut(s) 36, 333, 340, 404, 486
MlyI GAGTC 1 cut(s) 163
MnlI CCTC 5 cut(s) 64, 72, 189, 248, 267
MroXI GAANNNNTTC 1 cut(s) 36
MseI TTAA 2 cut(s) 42, 81
MslI CAYNNNNRTG 1 cut(s) 162
MspR9I CCNGG 1 cut(s) 242
MunI CAATTG 1 cut(s) 340
MvaI CCWGG 1 cut(s) 242
MwoI GCNNNNNNNGC 1 cut(s) 497
NdeI CATATG 1 cut(s) 49
NdeII GATC 3 cut(s) 366, 394, 445
NlaIII CATG 2 cut(s) 215, 500
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 166, 431
OliI CACNNNNGTG 1 cut(s) 162
PdmI GAANNNNTTC 1 cut(s) 36
PkrI GCNGC 1 cut(s) 266
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp124BI GAGCTC 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 1 cut(s) 357
PsuI RGATCY 1 cut(s) 394
RseI CAYNNNNRTG 1 cut(s) 162
SacI GAGCTC 1 cut(s) 420
SaqAI TTAA 2 cut(s) 42, 81
SatI GCNGC 1 cut(s) 265
Sau3AI GATC 3 cut(s) 366, 394, 445
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 2 cut(s) 360, 420
SetI ASST 7 cut(s) 87, 189, 259, 278, 301, 420, 444
SmiMI CAYNNNNRTG 1 cut(s) 162
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
SpeI ACTAGT 1 cut(s) 377
Sse9I AATT 5 cut(s) 36, 333, 340, 404, 486
SspMI CTAG 2 cut(s) 20, 378
SstI GAGCTC 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 240
StyI CCWWGG 1 cut(s) 216
TaiI ACGT 1 cut(s) 444
TasI AATT 5 cut(s) 36, 333, 340, 404, 486
Tru1I TTAA 2 cut(s) 42, 81
Tru9I TTAA 2 cut(s) 42, 81
TscAI CASTG 1 cut(s) 417
TseFI GTSAC 2 cut(s) 166, 431
TseI GCWGC 1 cut(s) 264
Tsp45I GTSAC 2 cut(s) 166, 431
TspDTI ATGAA 2 cut(s) 77, 417
TspRI CASTG 1 cut(s) 417
XapI RAATTY 1 cut(s) 36
XmnI GAANNNNTTC 1 cut(s) 36
XspI CTAG 2 cut(s) 20, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.