RchiOBHm_Chr6g0301191

Redoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017254
Physical Location & Seq
Forward (+)
61712036 .. 61713179
1144 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27050

Sequence Viewer

Length: 432 bp
ATGCATCCCTCTCGTACCTCAACCGAAACGACGTCGTTAAATCCGTCACTCTCTCCAGCCTCTGCAAGGGAAAGAGAGTCGTTTTTGGTGGGCGTAACGGCGCCGTTCTCGCCGGGTTGCACTCGGTTCGTCAAACGACTTAAATCGGCGAGGGAGAAAACTGCGGAGGTGATCGCCTGCTTCGCGGTCAGCGACGTTTTCGTGATGCGCGCCTGGGGAGAGATCCTGGCCGTTGGTGAGAGAGTGATGATGTTGTGCGACGGTCTAGGAGAGCTGACCAGGGCACTTGGCGTCTCTCTCAACGGAGCCGTTAAAGCTTGTCTCGGCTTAGGTGTGCGGTCCAGGAGGTTTTGCCTGGCTTCTTTCAACGGGGTGATCACAAACGTTGACTTTGACGAAGAATCTGATTTCTTCCCAGTAATTGTTCATTGA

Protein Analysis

143

Amino Acids

15.47

Weight (kDa)

7.65

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Redoxin PF08534 12 - 133 1.1e-12 Redoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017254)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g39680
prunus_persica Prupe.1G378100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0301191
rosa_laevigata RLG00000011269
rosa_multiflora Rmu_sc0002592.1_g000003
rosa_roxburghii Rroxscaffold_171G00441350 Rroxscaffold_7G00167060
rosa_rugosa Rorug06G0302300
rosa_samantha Rh6AG415600 Rh6BG422500 Rh6CG430100 Rh6DG415400 Rh6DG415500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 100
AccII CGCG 2 cut(s) 185, 210
AciI CCGC 3 cut(s) 164, 185, 337
AclI AACGTT 1 cut(s) 384
AclWI GGATC 1 cut(s) 217
AcoI YGGCCR 1 cut(s) 228
AcyI GRCGYC 3 cut(s) 32, 101, 291
AfaI GTAC 1 cut(s) 16
AfiI CCNNNNNNNGG 1 cut(s) 66
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 5 cut(s) 212, 225, 278, 341, 354
AluBI AGCT 2 cut(s) 274, 317
AluI AGCT 2 cut(s) 274, 317
Alw26I GTCTC 2 cut(s) 298, 326
AlwI GGATC 1 cut(s) 217
AlwNI CAGNNNCTG 1 cut(s) 62
AoxI GGCC 1 cut(s) 228
AspLEI GCGC 3 cut(s) 103, 210, 212
AspS9I GGNCC 1 cut(s) 339
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 3 cut(s) 181, 248, 385
AvaII GGWCC 1 cut(s) 339
BaeGI GKGCMC 1 cut(s) 286
BanI GGYRCC 1 cut(s) 100
BceAI ACGGC 4 cut(s) 88, 114, 215, 293
BcgI CGANNNNNNTGC 3 cut(s) 27, 109, 143
BciT130I CCWGG 5 cut(s) 214, 227, 280, 343, 356
BclI TGATCA 1 cut(s) 375
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 2 cut(s) 298, 326
BfaI CTAG 1 cut(s) 266
BfoI RGCGCY 1 cut(s) 104
Bme1390I CCNGG 6 cut(s) 114, 214, 227, 280, 343, 356
Bme18I GGWCC 1 cut(s) 339
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 2 cut(s) 102, 307
BmrFI CCNGG 6 cut(s) 114, 214, 227, 280, 343, 356
BmrI ACTGGG 1 cut(s) 410
BmsI GCATC 2 cut(s) 13, 195
BmuI ACTGGG 1 cut(s) 410
BpmI CTGGAG 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 328
BpuMI CCSGG 1 cut(s) 114
BsaHI GRCGYC 3 cut(s) 32, 101, 291
BsaJI CCNNGG 2 cut(s) 213, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 66
Bse1I ACTGG 1 cut(s) 416
BseBI CCWGG 5 cut(s) 214, 227, 280, 343, 356
BseDI CCNNGG 2 cut(s) 213, 279
BseGI GGATG 1 cut(s) 4
BseLI CCNNNNNNNGG 1 cut(s) 66
BseNI ACTGG 1 cut(s) 416
BsePI GCGCGC 1 cut(s) 208
BseSI GKGCMC 1 cut(s) 286
Bsh1236I CGCG 2 cut(s) 185, 210
BshFI GGCC 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 100
BsiSI CCGG 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 66
BsmAI GTCTC 2 cut(s) 298, 326
BsmBI CGTCTC 1 cut(s) 298
BsnI GGCC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 3 cut(s) 171, 222, 375
BspACI CCGC 3 cut(s) 164, 185, 337
BspANI GGCC 1 cut(s) 230
BspFNI CGCG 2 cut(s) 185, 210
BspLI GGNNCC 2 cut(s) 102, 307
BspPI GGATC 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 100
BsrI ACTGG 1 cut(s) 416
BssECI CCNNGG 2 cut(s) 213, 279
BssHII GCGCGC 1 cut(s) 208
BssMI GATC 3 cut(s) 171, 222, 375
BssNI GRCGYC 3 cut(s) 32, 101, 291
Bst2UI CCWGG 5 cut(s) 214, 227, 280, 343, 356
Bst4CI ACNGT 1 cut(s) 263
BstACI GRCGYC 3 cut(s) 32, 101, 291
BstC8I GCNNGC 2 cut(s) 178, 210
BstDEI CTNAG 1 cut(s) 328
BstENI CCTNNNNNAGG 1 cut(s) 64
BstF5I GGATG 1 cut(s) 4
BstFNI CGCG 2 cut(s) 185, 210
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 3 cut(s) 103, 210, 212
BstKTI GATC 3 cut(s) 174, 225, 378
BstMAI GTCTC 2 cut(s) 298, 326
BstMBI GATC 3 cut(s) 171, 222, 375
BstMWI GCNNNNNNNGC 3 cut(s) 109, 182, 314
BstNI CCWGG 5 cut(s) 214, 227, 280, 343, 356
BstSCI CCNGG 6 cut(s) 112, 212, 225, 278, 341, 354
BstSLI GKGCMC 1 cut(s) 286
BstUI CGCG 2 cut(s) 185, 210
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BsuRI GGCC 1 cut(s) 230
BtsCI GGATG 1 cut(s) 4
Cac8I GCNNGC 2 cut(s) 178, 210
CaiI CAGNNNCTG 1 cut(s) 62
CfoI GCGC 3 cut(s) 103, 210, 212
Cfr13I GGNCC 1 cut(s) 339
CseI GACGC 1 cut(s) 280
Csp6I GTAC 1 cut(s) 15
CviJI RGCY 7 cut(s) 59, 230, 274, 308, 317, 327, 359
CviKI_1 RGCY 7 cut(s) 59, 230, 274, 308, 317, 327, 359
CviQI GTAC 1 cut(s) 15
DdeI CTNAG 1 cut(s) 328
DinI GGCGCC 1 cut(s) 102
DpnI GATC 3 cut(s) 173, 224, 377
DpnII GATC 3 cut(s) 171, 222, 375
EaeI YGGCCR 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 339
EcoNI CCTNNNNNAGG 1 cut(s) 64
EcoRII CCWGG 5 cut(s) 212, 225, 278, 341, 354
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 102
EheI GGCGCC 1 cut(s) 102
Esp3I CGTCTC 1 cut(s) 298
FbaI TGATCA 1 cut(s) 375
FspBI CTAG 1 cut(s) 266
GlaI GCGC 3 cut(s) 102, 209, 211
GsuI CTGGAG 1 cut(s) 39
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 1 cut(s) 230
HapII CCGG 1 cut(s) 113
HgaI GACGC 1 cut(s) 280
HhaI GCGC 3 cut(s) 103, 210, 212
Hin1I GRCGYC 3 cut(s) 32, 101, 291
Hin6I GCGC 3 cut(s) 101, 208, 210
HinP1I GCGC 3 cut(s) 101, 208, 210
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 2 cut(s) 77, 401
HpaII CCGG 1 cut(s) 113
HphI GGTGA 3 cut(s) 181, 248, 385
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 1 cut(s) 406
Hpy188III TCNNGA 1 cut(s) 202
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 4 cut(s) 34, 37, 197, 263
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4IV ACGT 3 cut(s) 32, 195, 384
HpyCH4V TGCA 3 cut(s) 4, 65, 120
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 182, 314
HpyF3I CTNAG 1 cut(s) 328
HpySE526I ACGT 3 cut(s) 32, 195, 384
Hsp92I GRCGYC 3 cut(s) 32, 101, 291
HspAI GCGC 3 cut(s) 101, 208, 210
KasI GGCGCC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 375
Kzo9I GATC 3 cut(s) 171, 222, 375
LmnI GCTCC 1 cut(s) 305
LweI GCATC 2 cut(s) 13, 195
MaeI CTAG 1 cut(s) 266
MaeII ACGT 3 cut(s) 32, 195, 384
MaeIII GTNAC 2 cut(s) 45, 94
MalI GATC 3 cut(s) 173, 224, 377
MboI GATC 3 cut(s) 171, 222, 375
MboII GAAGA 2 cut(s) 403, 410
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 1 cut(s) 420
Mly113I GGCGCC 1 cut(s) 101
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 6 cut(s) 19, 28, 70, 144, 160, 339
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 38, 141, 312
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 6 cut(s) 114, 214, 227, 280, 343, 356
MvaI CCWGG 5 cut(s) 214, 227, 280, 343, 356
MvnI CGCG 2 cut(s) 185, 210
MwoI GCNNNNNNNGC 3 cut(s) 109, 182, 314
NarI GGCGCC 1 cut(s) 101
NciI CCSGG 1 cut(s) 114
NdeII GATC 3 cut(s) 171, 222, 375
NlaIV GGNNCC 2 cut(s) 102, 307
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 45
NsiI ATGCAT 1 cut(s) 6
PauI GCGCGC 1 cut(s) 208
PcsI WCGNNNNNNNCGW 2 cut(s) 41, 189
PfeI GAWTC 1 cut(s) 401
PfoI TCCNGGA 1 cut(s) 341
PleI GAGTC 1 cut(s) 85
PluTI GGCGCC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 85
Psp1406I AACGTT 1 cut(s) 384
Psp6I CCWGG 5 cut(s) 212, 225, 278, 341, 354
PspGI CCWGG 5 cut(s) 212, 225, 278, 341, 354
PspN4I GGNNCC 2 cut(s) 102, 307
PspPI GGNCC 1 cut(s) 339
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 1 cut(s) 222
PteI GCGCGC 1 cut(s) 208
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
SaqAI TTAA 3 cut(s) 38, 141, 312
Sau3AI GATC 3 cut(s) 171, 222, 375
Sau96I GGNCC 1 cut(s) 339
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 6 cut(s) 114, 214, 227, 280, 343, 356
SduI GDGCHC 1 cut(s) 286
SetI ASST 9 cut(s) 20, 35, 171, 198, 276, 319, 334, 350, 387
SfaNI GCATC 2 cut(s) 13, 195
SfoI GGCGCC 1 cut(s) 102
SinI GGWCC 1 cut(s) 339
Sse9I AATT 1 cut(s) 420
SsiI CCGC 3 cut(s) 164, 185, 337
SspDI GGCGCC 1 cut(s) 100
SspMI CTAG 1 cut(s) 266
StyD4I CCNGG 6 cut(s) 112, 212, 225, 278, 341, 354
TaaI ACNGT 1 cut(s) 263
TaiI ACGT 3 cut(s) 35, 198, 387
TasI AATT 1 cut(s) 420
TfiI GAWTC 1 cut(s) 401
Tru1I TTAA 3 cut(s) 38, 141, 312
Tru9I TTAA 3 cut(s) 38, 141, 312
TseFI GTSAC 1 cut(s) 45
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 1 cut(s) 416
TspGWI ACGGA 2 cut(s) 33, 318
VpaK11BI GGWCC 1 cut(s) 339
XagI CCTNNNNNAGG 1 cut(s) 64
XspI CTAG 1 cut(s) 266
ZraI GACGTC 1 cut(s) 33
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.