RchiOBHm_Chr6g0310621

Catalyzes the ferrous insertion into protoporphyrin IX

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004323
Physical Location & Seq
Forward (+)
67880188 .. 67883435
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27932

Sequence Viewer

Length: 1473 bp
ATGGACGCCGCCGTTTCATGCTCCGGTGCCCTTCCCCAAACGAACCTCTCCGGCTCCGACCTCTCCCGCTCAGATCATAAATTTCCGCTGGGTAGGTCTTCGTGCTCTTACGCGAGGTCAATTGGGGCGGTTTCTTGCAATAAAGGATTGCGAATCGTTCCGAAAAAGCTTGAATTCTTGTGCTCTGCTGCGTCGAGAGATGAAGAGAGGAATGAAATGGTGTTTGGGGCATCGCATTGCGAGAAGAAAAGCCCTCTTGGGGAGGCATTGTGTGTTGCTGCGGGTGTATGCACTTATGGTGGGACTGCTGTGGAGTCTCATCACTCCCACATTGCGGAAGAGAAGGTTGGAGTGCTGCTCCTGAATCTTGGAGGACCCGAGACGCTTGATGATGTTCAGCCGTTTCTGTTTAATCTATTTGCTGACCCGGATATCATACGCCTACCTAGGGCGTTTAGGTTTCTTCAGAAGCCACTGGCAAAATTGATTTCTGTGCTTCGAGCTCCTAAGAGCAAAGAAGGGTATGCGTCTATTGGAGGTGGTTCGCCCTTGCGTAAGATAACAGATGAGCAGGCAAATGCACTGAAAATGGCTTTGGAATCAAAGGACATGCCGGTGAATGTCTATGTGGGAATGCGGTACTGGTACCCATTCACTGAGGAAGCAGTTCATCAAATTAAGAGGGACAGAATAACCAGGCTTGTTGTCCTGCCTCTATATCCTCAATTCTCTATCTCTACTACTGGATCAAGCGTTCGTGTGCTCCAGAAGATTTTCAGGAATGATCCATATCTATCAAGGTTGCCTGTTTCAGTTATAAATTCATGGTATCAACGTGAAGGTTATATTAAGTCAATGGCTGACTTGATCTCAAAAGAGCTAGAGAGTTTTTCTGAGCCTGCACAGGTCATGATATTCTTCAGTGCCCACGGGGTACCACTCAGTTATGTGAAAGACTCTGGAGATCCATACAAAGATCAAATGGAGGAGTGTATCTTTCTAATAATGAAAGAGTTGAAAGCTAGAGGAATCAATAATGAACATACTCTTGCCTACCAGAGCCGAGTTGGTCCTGTACAATGGTTGAAGCCATACACTGATGAAGTTCTTGTTGAGCTTGGCCAGAAAGGAGTAAAAAGTCTTCTAGCTGTTCCTGTCAGCTTTGTGAGTGAGCACATAGAAACTCTTGAAGAGATAGATATGGAGTACAAGGAGTTGGCCCTCGAATCCGGCATTGAGAATTGGGGCCGAGTCCCTGCCCTTGGCCTTACCCCTTCATTTATAACAGATCTGGCAGATGCAGTTATAGAGGCTCTACCACTGGCAGCAGCCACTTCAACCCCAAAGACTACCCCAGCAGTTGATCATGACCCTATGAGATGGGCTATCAAAATGTTCTTTGGTTCATTTCTAGCATTCATTTTGTTCTTTTCACCAAAGATGCTAATGGCATTCAGGAACCACCTGATTTGA

Protein Analysis

490

Amino Acids

54.36

Weight (kDa)

6.44

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferrochelatase PF00762 115 - 438 1.6e-111 Ferrochelatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 818, 1283
Acc65I GGTACC 2 cut(s) 645, 934
AccB1I GGYRCC 3 cut(s) 26, 645, 934
AccBSI CCGCTC 1 cut(s) 69
AccII CGCG 1 cut(s) 113
AciI CCGC 7 cut(s) 9, 67, 86, 128, 281, 335, 637
AclWI GGATC 3 cut(s) 754, 779, 959
AcoI YGGCCR 1 cut(s) 1120
AcsI RAATTY 3 cut(s) 80, 173, 820
AcuI CTGAAG 2 cut(s) 449, 904
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 5 cut(s) 641, 647, 936, 1077, 1208
AfiI CCNNNNNNNGG 4 cut(s) 259, 334, 448, 1262
AgsI TTSAA 5 cut(s) 173, 1018, 1087, 1190, 1338
AjnI CCWGG 1 cut(s) 695
AjuI GAANNNNNNNTTGG 6 cut(s) 207, 239, 330, 362, 578, 610
AloI GAACNNNNNNTCC 2 cut(s) 738, 770
AluBI AGCT 7 cut(s) 169, 503, 880, 1022, 1117, 1148, 1161
AluI AGCT 7 cut(s) 169, 503, 880, 1022, 1117, 1148, 1161
Alw21I GWGCWC 5 cut(s) 107, 185, 505, 765, 1176
Alw26I GTCTC 2 cut(s) 321, 374
AlwI GGATC 3 cut(s) 754, 779, 959
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 4 cut(s) 1120, 1218, 1246, 1264
ApeKI GCWGC 5 cut(s) 188, 278, 355, 1325, 1328
ApoI RAATTY 3 cut(s) 80, 173, 820
Asp700I GAANNNNTTC 2 cut(s) 666, 773
Asp718I GGTACC 2 cut(s) 645, 934
AspA2I CCTAGG 1 cut(s) 446
AspS9I GGNCC 4 cut(s) 374, 1070, 1219, 1246
AsuC2I CCSGG 1 cut(s) 428
AsuHPI GGTGA 2 cut(s) 628, 1425
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 2 cut(s) 374, 1070
AvrII CCTAGG 1 cut(s) 446
BaeGI GKGCMC 2 cut(s) 31, 928
BalI TGGCCA 1 cut(s) 1122
BanI GGYRCC 3 cut(s) 26, 645, 934
BanII GRGCYC 1 cut(s) 505
BarI GAAGNNNNNNTAC 2 cut(s) 1125, 1157
BbsI GAAGAC 2 cut(s) 90, 1133
Bbv12I GWGCWC 5 cut(s) 107, 185, 505, 765, 1176
BbvI GCAGC 5 cut(s) 175, 265, 342, 1337, 1340
BccI CCATC 1 cut(s) 1374
BceAI ACGGC 1 cut(s) 385
BciT130I CCWGG 1 cut(s) 697
BclI TGATCA 1 cut(s) 1363
BcnI CCSGG 1 cut(s) 428
BcoDI GTCTC 2 cut(s) 321, 374
BfaI CTAG 5 cut(s) 447, 881, 1023, 1145, 1412
BglII AGATCT 1 cut(s) 1288
BisI GCNGC 6 cut(s) 9, 189, 279, 356, 1326, 1329
BlnI CCTAGG 1 cut(s) 446
BlsI GCNGC 6 cut(s) 10, 190, 280, 357, 1327, 1330
Bme1390I CCNGG 2 cut(s) 428, 697
Bme18I GGWCC 2 cut(s) 374, 1070
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 4 cut(s) 374, 1070, 1219, 1246
BmiI GGNNCC 7 cut(s) 28, 55, 376, 647, 936, 1247, 1460
BmrFI CCNGG 2 cut(s) 428, 697
BmsI GCATC 3 cut(s) 239, 1288, 1431
BpiI GAAGAC 2 cut(s) 90, 1133
BplI GAGNNNNNCTC 2 cut(s) 342, 374
BpmI CTGGAG 2 cut(s) 749, 981
BpuMI CCSGG 1 cut(s) 428
BsaBI GATNNNNATC 1 cut(s) 789
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 3 cut(s) 446, 928, 1261
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 334, 448, 1262
Bse118I RCCGGY 1 cut(s) 613
Bse1I ACTGG 4 cut(s) 480, 647, 748, 1326
Bse3DI GCAATG 2 cut(s) 235, 330
Bse8I GATNNNNATC 1 cut(s) 789
BseBI CCWGG 1 cut(s) 697
BseDI CCNNGG 3 cut(s) 446, 928, 1261
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 4 cut(s) 259, 334, 448, 1262
BseMI GCAATG 2 cut(s) 235, 330
BseMII CTCAG 4 cut(s) 84, 648, 885, 955
BseNI ACTGG 4 cut(s) 480, 647, 748, 1326
BseRI GAGGAG 1 cut(s) 1001
BseSI GKGCMC 2 cut(s) 31, 928
BseXI GCAGC 5 cut(s) 175, 265, 342, 1337, 1340
BseYI CCCAGC 2 cut(s) 88, 1354
BsgI GTGCAG 1 cut(s) 885
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 4 cut(s) 1122, 1220, 1248, 1266
BshNI GGYRCC 3 cut(s) 26, 645, 934
BsiHKAI GWGCWC 5 cut(s) 107, 185, 505, 765, 1176
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 5 cut(s) 24, 51, 428, 614, 1230
BslFI GGGAC 3 cut(s) 316, 698, 1238
BslI CCNNNNNNNGG 4 cut(s) 259, 334, 448, 1262
BsmAI GTCTC 2 cut(s) 321, 374
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 3 cut(s) 316, 698, 1238
BsmI GAATGC 3 cut(s) 639, 1415, 1451
BsnI GGCC 4 cut(s) 1122, 1220, 1248, 1266
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 7 cut(s) 31, 107, 185, 505, 765, 928, 1176
Bsp1407I TGTACA 1 cut(s) 1075
Bsp143I GATC 8 cut(s) 73, 746, 784, 867, 964, 976, 1288, 1363
BspACI CCGC 7 cut(s) 9, 67, 86, 128, 281, 335, 637
BspANI GGCC 4 cut(s) 1122, 1220, 1248, 1266
BspCNI CTCAG 4 cut(s) 83, 649, 886, 954
BspFNI CGCG 1 cut(s) 113
BspHI TCATGA 2 cut(s) 909, 1366
BspLI GGNNCC 7 cut(s) 28, 55, 376, 647, 936, 1247, 1460
BspPI GGATC 3 cut(s) 754, 779, 959
BspT107I GGYRCC 3 cut(s) 26, 645, 934
BsrBI CCGCTC 1 cut(s) 69
BsrDI GCAATG 2 cut(s) 235, 330
BsrFI RCCGGY 1 cut(s) 613
BsrGI TGTACA 1 cut(s) 1075
BsrI ACTGG 4 cut(s) 480, 647, 748, 1326
BssAI RCCGGY 1 cut(s) 613
BssECI CCNNGG 3 cut(s) 446, 928, 1261
BssMI GATC 8 cut(s) 73, 746, 784, 867, 964, 976, 1288, 1363
BssNI GRCGYC 1 cut(s) 6
BssT1I CCWWGG 2 cut(s) 446, 1261
Bst2UI CCWGG 1 cut(s) 697
Bst6I CTCTTC 3 cut(s) 198, 333, 1185
BstACI GRCGYC 1 cut(s) 6
BstAUI TGTACA 1 cut(s) 1075
BstC8I GCNNGC 2 cut(s) 573, 900
BstDEI CTNAG 5 cut(s) 70, 507, 657, 894, 941
BstDSI CCRYGG 1 cut(s) 928
BstFNI CGCG 1 cut(s) 113
BstKTI GATC 8 cut(s) 76, 749, 787, 870, 967, 979, 1291, 1366
BstMAI GTCTC 2 cut(s) 321, 374
BstMBI GATC 8 cut(s) 73, 746, 784, 867, 964, 976, 1288, 1363
BstNI CCWGG 1 cut(s) 697
BstNSI RCATGY 1 cut(s) 613
BstSCI CCNGG 2 cut(s) 426, 695
BstSLI GKGCMC 2 cut(s) 31, 928
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 5 cut(s) 175, 265, 342, 1337, 1340
BstV2I GAAGAC 2 cut(s) 90, 1133
BstX2I RGATCY 2 cut(s) 964, 1288
BstYI RGATCY 2 cut(s) 964, 1288
BsuRI GGCC 4 cut(s) 1122, 1220, 1248, 1266
BtgI CCRYGG 1 cut(s) 928
BtgZI GCGATG 1 cut(s) 216
BtsIMutI CAGTG 6 cut(s) 473, 581, 654, 928, 1095, 1319
Cac8I GCNNGC 2 cut(s) 573, 900
CciI TCATGA 2 cut(s) 909, 1366
Cfr10I RCCGGY 1 cut(s) 613
Cfr13I GGNCC 4 cut(s) 374, 1070, 1219, 1246
CseI GACGC 4 cut(s) 14, 180, 391, 516
Csp6I GTAC 5 cut(s) 640, 646, 935, 1076, 1207
CspCI CAANNNNNGTGG 1 cut(s) 1451
CviAII CATG 5 cut(s) 18, 610, 825, 910, 1367
CviQI GTAC 5 cut(s) 640, 646, 935, 1076, 1207
DdeI CTNAG 5 cut(s) 70, 507, 657, 894, 941
DpnI GATC 8 cut(s) 75, 748, 786, 869, 966, 978, 1290, 1365
DpnII GATC 8 cut(s) 73, 746, 784, 867, 964, 976, 1288, 1363
EaeI YGGCCR 1 cut(s) 1120
Eam1104I CTCTTC 3 cut(s) 198, 333, 1185
EarI CTCTTC 3 cut(s) 198, 333, 1185
Ecl136II GAGCTC 1 cut(s) 503
Eco130I CCWWGG 2 cut(s) 446, 1261
Eco24I GRGCYC 1 cut(s) 505
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 2 cut(s) 374, 1070
Eco53kI GAGCTC 1 cut(s) 503
Eco57I CTGAAG 2 cut(s) 449, 904
Eco88I CYCGRG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 374
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 695
EcoRV GATATC 1 cut(s) 433
EcoT14I CCWWGG 2 cut(s) 446, 1261
EcoT38I GRGCYC 1 cut(s) 505
ErhI CCWWGG 2 cut(s) 446, 1261
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 5 cut(s) 21, 613, 828, 913, 1370
FaqI GGGAC 3 cut(s) 316, 698, 1238
FatI CATG 5 cut(s) 17, 609, 824, 909, 1366
FauI CCCGC 2 cut(s) 74, 274
FbaI TGATCA 1 cut(s) 1363
Fnu4HI GCNGC 6 cut(s) 9, 189, 279, 356, 1326, 1329
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 6 cut(s) 9, 189, 279, 356, 1326, 1329
FspBI CTAG 5 cut(s) 447, 881, 1023, 1145, 1412
GluI GCNGC 6 cut(s) 9, 189, 279, 356, 1326, 1329
GsaI CCCAGC 2 cut(s) 92, 1358
GsuI CTGGAG 2 cut(s) 749, 981
HaeIII GGCC 4 cut(s) 1122, 1220, 1248, 1266
HapII CCGG 5 cut(s) 24, 51, 428, 614, 1230
HgaI GACGC 4 cut(s) 14, 180, 391, 516
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 5 cut(s) 21, 613, 828, 913, 1370
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 8 cut(s) 153, 314, 364, 599, 956, 1029, 1226, 1251
HpaII CCGG 5 cut(s) 24, 51, 428, 614, 1230
HphI GGTGA 2 cut(s) 628, 1425
Hpy188I TCNGA 5 cut(s) 58, 73, 162, 468, 895
Hpy188III TCNNGA 9 cut(s) 195, 361, 766, 778, 910, 960, 1187, 1367, 1456
Hpy99I CGWCG 1 cut(s) 196
HpyAV CCTTC 5 cut(s) 41, 337, 512, 833, 1284
HpyCH4IV ACGT 1 cut(s) 835
HpyCH4V TGCA 5 cut(s) 138, 291, 581, 902, 1301
HpyF3I CTNAG 5 cut(s) 70, 507, 657, 894, 941
HpySE526I ACGT 1 cut(s) 835
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 5 cut(s) 21, 613, 828, 913, 1370
KpnI GGTACC 2 cut(s) 649, 938
Ksp22I TGATCA 1 cut(s) 1363
Kzo9I GATC 8 cut(s) 73, 746, 784, 867, 964, 976, 1288, 1363
LmnI GCTCC 5 cut(s) 26, 59, 363, 508, 768
Lsp1109I GCAGC 5 cut(s) 175, 265, 342, 1337, 1340
LweI GCATC 3 cut(s) 239, 1288, 1431
MaeI CTAG 5 cut(s) 447, 881, 1023, 1145, 1412
MaeII ACGT 1 cut(s) 835
MalI GATC 8 cut(s) 75, 748, 786, 869, 966, 978, 1290, 1365
MbiI CCGCTC 1 cut(s) 69
MboI GATC 8 cut(s) 73, 746, 784, 867, 964, 976, 1288, 1363
MboII GAAGA 9 cut(s) 90, 215, 256, 350, 455, 781, 910, 1133, 1202
MfeI CAATTG 1 cut(s) 120
MflI RGATCY 2 cut(s) 964, 1288
MhlI GDGCHC 7 cut(s) 31, 107, 185, 505, 765, 928, 1176
MlsI TGGCCA 1 cut(s) 1122
MluCI AATT 8 cut(s) 80, 120, 173, 482, 675, 725, 820, 1240
MluNI TGGCCA 1 cut(s) 1122
MlyI GAGTC 3 cut(s) 323, 950, 1260
MmeI TCCRAC 2 cut(s) 81, 328
Mox20I TGGCCA 1 cut(s) 1122
MroXI GAANNNNTTC 2 cut(s) 666, 773
MscI TGGCCA 1 cut(s) 1122
MseI TTAA 3 cut(s) 411, 678, 849
MslI CAYNNNNRTG 1 cut(s) 614
Msp20I TGGCCA 1 cut(s) 1122
MspA1I CMGCKG 1 cut(s) 88
MspI CCGG 5 cut(s) 24, 51, 428, 614, 1230
MspR9I CCNGG 2 cut(s) 428, 697
MunI CAATTG 1 cut(s) 120
Mva1269I GAATGC 3 cut(s) 639, 1415, 1451
MvaI CCWGG 1 cut(s) 697
MvnI CGCG 1 cut(s) 113
NciI CCSGG 1 cut(s) 428
NdeII GATC 8 cut(s) 73, 746, 784, 867, 964, 976, 1288, 1363
NlaIII CATG 5 cut(s) 21, 613, 828, 913, 1370
NlaIV GGNNCC 7 cut(s) 28, 55, 376, 647, 936, 1247, 1460
NmeAIII GCCGAG 2 cut(s) 1088, 1274
NspI RCATGY 1 cut(s) 613
PagI TCATGA 2 cut(s) 909, 1366
PctI GAATGC 3 cut(s) 639, 1415, 1451
PdmI GAANNNNTTC 2 cut(s) 666, 773
PfeI GAWTC 5 cut(s) 153, 364, 599, 1029, 1226
PkrI GCNGC 6 cut(s) 10, 190, 280, 357, 1327, 1330
PleI GAGTC 3 cut(s) 322, 950, 1259
PpsI GAGTC 3 cut(s) 322, 950, 1259
PpuMI RGGWCCY 1 cut(s) 374
PsiI TTATAA 2 cut(s) 818, 1283
Psp124BI GAGCTC 1 cut(s) 505
Psp5II RGGWCCY 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 695
PspFI CCCAGC 2 cut(s) 88, 1354
PspGI CCWGG 1 cut(s) 695
PspN4I GGNNCC 7 cut(s) 28, 55, 376, 647, 936, 1247, 1460
PspPI GGNCC 4 cut(s) 374, 1070, 1219, 1246
PspPPI RGGWCCY 1 cut(s) 374
PsuI RGATCY 2 cut(s) 964, 1288
RsaI GTAC 5 cut(s) 641, 647, 936, 1077, 1208
RsaNI GTAC 5 cut(s) 640, 646, 935, 1076, 1207
RseI CAYNNNNRTG 1 cut(s) 614
SacI GAGCTC 1 cut(s) 505
SaqAI TTAA 3 cut(s) 411, 678, 849
SatI GCNGC 6 cut(s) 9, 189, 279, 356, 1326, 1329
Sau3AI GATC 8 cut(s) 73, 746, 784, 867, 964, 976, 1288, 1363
Sau96I GGNCC 4 cut(s) 374, 1070, 1219, 1246
SchI GAGTC 3 cut(s) 323, 950, 1260
ScrFI CCNGG 2 cut(s) 428, 697
SduI GDGCHC 7 cut(s) 31, 107, 185, 505, 765, 928, 1176
SfaNI GCATC 3 cut(s) 239, 1288, 1431
SinI GGWCC 2 cut(s) 374, 1070
SmiMI CAYNNNNRTG 1 cut(s) 614
Sse9I AATT 8 cut(s) 80, 120, 173, 482, 675, 725, 820, 1240
SsiI CCGC 7 cut(s) 9, 67, 86, 128, 281, 335, 637
SspMI CTAG 5 cut(s) 447, 881, 1023, 1145, 1412
SstI GAGCTC 1 cut(s) 505
StyD4I CCNGG 2 cut(s) 426, 695
StyI CCWWGG 2 cut(s) 446, 1261
TaiI ACGT 1 cut(s) 838
TaqI TCGA 3 cut(s) 194, 499, 1224
TasI AATT 8 cut(s) 80, 120, 173, 482, 675, 725, 820, 1240
TatI WGTACW 2 cut(s) 1075, 1206
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 5 cut(s) 153, 364, 599, 1029, 1226
Tru1I TTAA 3 cut(s) 411, 678, 849
Tru9I TTAA 3 cut(s) 411, 678, 849
TscAI CASTG 6 cut(s) 480, 588, 661, 928, 1102, 1326
TseI GCWGC 5 cut(s) 188, 278, 355, 1325, 1328
TspRI CASTG 6 cut(s) 480, 588, 661, 928, 1102, 1326
VpaK11BI GGWCC 2 cut(s) 374, 1070
XapI RAATTY 3 cut(s) 80, 173, 820
XceI RCATGY 1 cut(s) 613
XcmI CCANNNNNNNNNTGG 1 cut(s) 1064
XmaJI CCTAGG 1 cut(s) 446
XmnI GAANNNNTTC 2 cut(s) 666, 773
XspI CTAG 5 cut(s) 447, 881, 1023, 1145, 1412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.