Rh6BG505000

Catalyzes the ferrous insertion into protoporphyrin IX

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004323
Physical Location & Seq
Forward (+)
70316498 .. 70322156
5659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG505000.1

Sequence Viewer

Length: 1257 bp
ATGGTGTTTGGGGCATCGCATTGCGAGAAGAAAAGCCCTGTTGGGGAGGCATTGTGTGTTGCTGCGGGTGTATGCACTTACGGTGGGACTGCTGTGGAGTCTCATCACTCCCACATTGCGGAAGAGAAGGTTGGAGTGCTGCTCCTGAATCTTGGAGGACCCGAGACGCTTGATGATGTTCAGCCGTTTCTGTTTAATCTATTCGCTGACCCGGATATCATACGCCTACCTAGGGCGTTTAGGTTTCTTCAGAAGCCACTGGCGAAATTGATTTCTGTGCTTCGAGCTCCTAAGAGCAAAGAAGGGTATGCGTCTATTGGAGGTGGTTCGCCCTTGCGTAAGATAACAGATGAGCAGGCAAATGCACTGAAAATGGCTTTGGAATCAAAGGACATGCCGGTGAATGTCTATGTGGGAATGCGGTACTGGTACCCGTTCACTGAGGAAGCAGTTCATCAAATTAAGAGGGACAGAATAACCAGGCTTGTTGTCCTGCCTCTATATCCTCAATTCTCTATCTCTACAACTGGATCAAGCGTTCGTGTGCTCCAGAAGATTTTCAGGAACGATCCTTATCTATCAAGGTTGCCTGTTTCAGTTATAAATTCATGGTATCAACGTGAAGGTTATATTAAGTCAATGGCTGACTTGATCTCAAAAGAGATAGAGAGTTTTTCTGAGCCTGCACAGGTCATGATATTCTTCAGTGCCCATGGGGTACCACTCAGTTATGTGAAGGACTCTGGAGATCCATACAAAGATCAAATGGAGGAGTGTATCTTTCTAATAATGAAAGAGTTGAAAGCTAGAGGAATCAATAATGAACATACTCTTGCCTACCAGAGCCGAGTTGGTCCTGTACAATGGTTGAAGCCATACACTGATGAAGTTCTTGTTGAGCTTGGCCAGAAAGGAGTAAAAAGTCTTCTAGCTGTTCCAGTCAGCTTTGTGAGTGAGCACATAGAAACTCTTGAAGAGATAGATATGGAGTACAAGGAGTTGGCCCTCGAATCTGGCATTGAGAATTGGGGCCGAGTCCCTGCCCTTGGCCTTACCCCTTCATTTATAACAGATCTGGCAGATGCAGTTATAGAGGCTCTACCACTGGCAGCAGCCACTTCAACCCCAAAGACTACCCCAGCAGTTGATCATGACCCTATGAGATGGGCTATCAAAATGTTCTTTGGTTCATTTCTAGCATTCATTTTGTTCTTTTCACCAAAGATGCTAATGGCATTCAGGAACCACCTGATTTGA

Protein Analysis

418

Amino Acids

46.69

Weight (kDa)

6.1

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferrochelatase PF00762 43 - 366 1.5e-111 Ferrochelatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 602, 1067
Acc65I GGTACC 2 cut(s) 429, 718
AccB1I GGYRCC 2 cut(s) 429, 718
AciI CCGC 3 cut(s) 65, 119, 421
AclWI GGATC 3 cut(s) 538, 563, 743
AcoI YGGCCR 1 cut(s) 904
AcsI RAATTY 1 cut(s) 604
AcuI CTGAAG 2 cut(s) 233, 688
AfaI GTAC 5 cut(s) 425, 431, 720, 861, 992
AfiI CCNNNNNNNGG 4 cut(s) 43, 118, 232, 1046
AgsI TTSAA 4 cut(s) 802, 871, 974, 1122
AjnI CCWGG 1 cut(s) 479
AjuI GAANNNNNNNTTGG 4 cut(s) 114, 146, 362, 394
AloI GAACNNNNNNTCC 2 cut(s) 522, 554
AluBI AGCT 5 cut(s) 287, 806, 901, 932, 945
AluI AGCT 5 cut(s) 287, 806, 901, 932, 945
Alw21I GWGCWC 3 cut(s) 289, 549, 960
Alw26I GTCTC 2 cut(s) 105, 158
AlwI GGATC 3 cut(s) 538, 563, 743
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 4 cut(s) 904, 1002, 1030, 1048
ApeKI GCWGC 4 cut(s) 62, 139, 1109, 1112
ApoI RAATTY 1 cut(s) 604
Asp700I GAANNNNTTC 2 cut(s) 450, 557
Asp718I GGTACC 2 cut(s) 429, 718
AspA2I CCTAGG 1 cut(s) 230
AspS9I GGNCC 4 cut(s) 158, 854, 1003, 1030
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 412, 1209
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 2 cut(s) 158, 854
AvrII CCTAGG 1 cut(s) 230
BaeGI GKGCMC 1 cut(s) 712
BalI TGGCCA 1 cut(s) 906
BanI GGYRCC 2 cut(s) 429, 718
BanII GRGCYC 1 cut(s) 289
BarI GAAGNNNNNNTAC 2 cut(s) 909, 941
BbsI GAAGAC 1 cut(s) 917
Bbv12I GWGCWC 3 cut(s) 289, 549, 960
BbvI GCAGC 4 cut(s) 49, 126, 1121, 1124
BccI CCATC 1 cut(s) 1158
BceAI ACGGC 1 cut(s) 169
BciT130I CCWGG 1 cut(s) 481
BclI TGATCA 1 cut(s) 1147
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 2 cut(s) 105, 158
BfaI CTAG 4 cut(s) 231, 807, 929, 1196
BglII AGATCT 1 cut(s) 1072
BisI GCNGC 4 cut(s) 63, 140, 1110, 1113
BlnI CCTAGG 1 cut(s) 230
BlsI GCNGC 4 cut(s) 64, 141, 1111, 1114
Bme1390I CCNGG 2 cut(s) 212, 481
Bme18I GGWCC 2 cut(s) 158, 854
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 4 cut(s) 158, 854, 1003, 1030
BmiI GGNNCC 5 cut(s) 160, 431, 720, 1031, 1244
BmrFI CCNGG 2 cut(s) 212, 481
BmsI GCATC 3 cut(s) 23, 1072, 1215
BpiI GAAGAC 1 cut(s) 917
BplI GAGNNNNNCTC 2 cut(s) 126, 158
BpmI CTGGAG 2 cut(s) 533, 765
BpuMI CCSGG 1 cut(s) 212
BsaBI GATNNNNATC 1 cut(s) 573
BsaJI CCNNGG 3 cut(s) 230, 712, 1045
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 118, 232, 1046
Bse118I RCCGGY 1 cut(s) 397
Bse1I ACTGG 5 cut(s) 264, 431, 532, 938, 1110
Bse3DI GCAATG 2 cut(s) 19, 114
Bse8I GATNNNNATC 1 cut(s) 573
BseBI CCWGG 1 cut(s) 481
BseDI CCNNGG 3 cut(s) 230, 712, 1045
BseJI GATNNNNATC 1 cut(s) 573
BseLI CCNNNNNNNGG 4 cut(s) 43, 118, 232, 1046
BseMI GCAATG 2 cut(s) 19, 114
BseMII CTCAG 3 cut(s) 432, 669, 739
BseNI ACTGG 5 cut(s) 264, 431, 532, 938, 1110
BseRI GAGGAG 1 cut(s) 785
BseSI GKGCMC 1 cut(s) 712
BseXI GCAGC 4 cut(s) 49, 126, 1121, 1124
BseYI CCCAGC 1 cut(s) 1138
BsgI GTGCAG 1 cut(s) 669
BshFI GGCC 4 cut(s) 906, 1004, 1032, 1050
BshNI GGYRCC 2 cut(s) 429, 718
BsiHKAI GWGCWC 3 cut(s) 289, 549, 960
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 2 cut(s) 212, 398
BslFI GGGAC 3 cut(s) 100, 482, 1022
BslI CCNNNNNNNGG 4 cut(s) 43, 118, 232, 1046
BsmAI GTCTC 2 cut(s) 105, 158
BsmBI CGTCTC 1 cut(s) 158
BsmFI GGGAC 3 cut(s) 100, 482, 1022
BsmI GAATGC 3 cut(s) 423, 1199, 1235
BsnI GGCC 4 cut(s) 906, 1004, 1032, 1050
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 4 cut(s) 289, 549, 712, 960
Bsp1407I TGTACA 1 cut(s) 859
Bsp143I GATC 7 cut(s) 530, 568, 651, 748, 760, 1072, 1147
Bsp19I CCATGG 1 cut(s) 712
BspACI CCGC 3 cut(s) 65, 119, 421
BspANI GGCC 4 cut(s) 906, 1004, 1032, 1050
BspCNI CTCAG 3 cut(s) 433, 670, 738
BspHI TCATGA 2 cut(s) 693, 1150
BspLI GGNNCC 5 cut(s) 160, 431, 720, 1031, 1244
BspPI GGATC 3 cut(s) 538, 563, 743
BspT107I GGYRCC 2 cut(s) 429, 718
BsrDI GCAATG 2 cut(s) 19, 114
BsrFI RCCGGY 1 cut(s) 397
BsrGI TGTACA 1 cut(s) 859
BsrI ACTGG 5 cut(s) 264, 431, 532, 938, 1110
BssAI RCCGGY 1 cut(s) 397
BssECI CCNNGG 3 cut(s) 230, 712, 1045
BssMI GATC 7 cut(s) 530, 568, 651, 748, 760, 1072, 1147
BssT1I CCWWGG 3 cut(s) 230, 712, 1045
Bst2UI CCWGG 1 cut(s) 481
Bst4CI ACNGT 1 cut(s) 83
Bst6I CTCTTC 2 cut(s) 117, 969
BstAUI TGTACA 1 cut(s) 859
BstC8I GCNNGC 2 cut(s) 357, 684
BstDEI CTNAG 4 cut(s) 291, 441, 678, 725
BstDSI CCRYGG 1 cut(s) 712
BstKTI GATC 7 cut(s) 533, 571, 654, 751, 763, 1075, 1150
BstMAI GTCTC 2 cut(s) 105, 158
BstMBI GATC 7 cut(s) 530, 568, 651, 748, 760, 1072, 1147
BstNI CCWGG 1 cut(s) 481
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 2 cut(s) 210, 479
BstSLI GKGCMC 1 cut(s) 712
BstV1I GCAGC 4 cut(s) 49, 126, 1121, 1124
BstV2I GAAGAC 1 cut(s) 917
BstX2I RGATCY 2 cut(s) 748, 1072
BstYI RGATCY 2 cut(s) 748, 1072
BsuRI GGCC 4 cut(s) 906, 1004, 1032, 1050
BtgI CCRYGG 1 cut(s) 712
BtsIMutI CAGTG 6 cut(s) 257, 365, 438, 712, 879, 1103
Cac8I GCNNGC 2 cut(s) 357, 684
CciI TCATGA 2 cut(s) 693, 1150
Cfr10I RCCGGY 1 cut(s) 397
Cfr13I GGNCC 4 cut(s) 158, 854, 1003, 1030
CseI GACGC 2 cut(s) 175, 300
Csp6I GTAC 5 cut(s) 424, 430, 719, 860, 991
CspCI CAANNNNNGTGG 1 cut(s) 1235
CviAII CATG 5 cut(s) 394, 609, 694, 713, 1151
CviQI GTAC 5 cut(s) 424, 430, 719, 860, 991
DdeI CTNAG 4 cut(s) 291, 441, 678, 725
DpnI GATC 7 cut(s) 532, 570, 653, 750, 762, 1074, 1149
DpnII GATC 7 cut(s) 530, 568, 651, 748, 760, 1072, 1147
EaeI YGGCCR 1 cut(s) 904
Eam1104I CTCTTC 2 cut(s) 117, 969
EarI CTCTTC 2 cut(s) 117, 969
Ecl136II GAGCTC 1 cut(s) 287
Eco130I CCWWGG 3 cut(s) 230, 712, 1045
Eco24I GRGCYC 1 cut(s) 289
Eco32I GATATC 1 cut(s) 217
Eco47I GGWCC 2 cut(s) 158, 854
Eco53kI GAGCTC 1 cut(s) 287
Eco57I CTGAAG 2 cut(s) 233, 688
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 287
EcoO109I RGGNCCY 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 479
EcoRV GATATC 1 cut(s) 217
EcoT14I CCWWGG 3 cut(s) 230, 712, 1045
EcoT38I GRGCYC 1 cut(s) 289
ErhI CCWWGG 3 cut(s) 230, 712, 1045
Esp3I CGTCTC 1 cut(s) 158
FaeI CATG 5 cut(s) 397, 612, 697, 716, 1154
FaqI GGGAC 3 cut(s) 100, 482, 1022
FatI CATG 5 cut(s) 393, 608, 693, 712, 1150
FauI CCCGC 1 cut(s) 58
FbaI TGATCA 1 cut(s) 1147
Fnu4HI GCNGC 4 cut(s) 63, 140, 1110, 1113
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 4 cut(s) 63, 140, 1110, 1113
FspBI CTAG 4 cut(s) 231, 807, 929, 1196
GluI GCNGC 4 cut(s) 63, 140, 1110, 1113
GsaI CCCAGC 1 cut(s) 1142
GsuI CTGGAG 2 cut(s) 533, 765
HaeIII GGCC 4 cut(s) 906, 1004, 1032, 1050
HapII CCGG 2 cut(s) 212, 398
HgaI GACGC 2 cut(s) 175, 300
Hin1II CATG 5 cut(s) 397, 612, 697, 716, 1154
HinfI GANTC 7 cut(s) 98, 148, 383, 740, 813, 1010, 1035
HpaII CCGG 2 cut(s) 212, 398
HphI GGTGA 2 cut(s) 412, 1209
Hpy166II GTNNAC 1 cut(s) 438
Hpy188I TCNGA 2 cut(s) 252, 679
Hpy188III TCNNGA 8 cut(s) 145, 550, 562, 694, 744, 971, 1151, 1240
Hpy8I GTNNAC 1 cut(s) 438
HpyAV CCTTC 5 cut(s) 121, 296, 617, 730, 1068
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 1 cut(s) 619
HpyCH4V TGCA 4 cut(s) 75, 365, 686, 1085
HpyF3I CTNAG 4 cut(s) 291, 441, 678, 725
HpySE526I ACGT 1 cut(s) 619
Hsp92II CATG 5 cut(s) 397, 612, 697, 716, 1154
KpnI GGTACC 2 cut(s) 433, 722
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 7 cut(s) 530, 568, 651, 748, 760, 1072, 1147
LmnI GCTCC 3 cut(s) 147, 292, 552
Lsp1109I GCAGC 4 cut(s) 49, 126, 1121, 1124
LweI GCATC 3 cut(s) 23, 1072, 1215
MaeI CTAG 4 cut(s) 231, 807, 929, 1196
MaeII ACGT 1 cut(s) 619
MalI GATC 7 cut(s) 532, 570, 653, 750, 762, 1074, 1149
MboI GATC 7 cut(s) 530, 568, 651, 748, 760, 1072, 1147
MboII GAAGA 7 cut(s) 40, 134, 239, 565, 694, 917, 986
MflI RGATCY 2 cut(s) 748, 1072
MhlI GDGCHC 4 cut(s) 289, 549, 712, 960
MlsI TGGCCA 1 cut(s) 906
MluCI AATT 5 cut(s) 266, 459, 509, 604, 1024
MluNI TGGCCA 1 cut(s) 906
MlyI GAGTC 3 cut(s) 107, 734, 1044
MmeI TCCRAC 1 cut(s) 112
Mox20I TGGCCA 1 cut(s) 906
MroXI GAANNNNTTC 2 cut(s) 450, 557
MscI TGGCCA 1 cut(s) 906
MseI TTAA 3 cut(s) 195, 462, 633
MslI CAYNNNNRTG 1 cut(s) 398
Msp20I TGGCCA 1 cut(s) 906
MspI CCGG 2 cut(s) 212, 398
MspR9I CCNGG 2 cut(s) 212, 481
Mva1269I GAATGC 3 cut(s) 423, 1199, 1235
MvaI CCWGG 1 cut(s) 481
NciI CCSGG 1 cut(s) 212
NcoI CCATGG 1 cut(s) 712
NdeII GATC 7 cut(s) 530, 568, 651, 748, 760, 1072, 1147
NlaIII CATG 5 cut(s) 397, 612, 697, 716, 1154
NlaIV GGNNCC 5 cut(s) 160, 431, 720, 1031, 1244
NmeAIII GCCGAG 2 cut(s) 872, 1058
NspI RCATGY 1 cut(s) 397
PagI TCATGA 2 cut(s) 693, 1150
PctI GAATGC 3 cut(s) 423, 1199, 1235
PdmI GAANNNNTTC 2 cut(s) 450, 557
PfeI GAWTC 4 cut(s) 148, 383, 813, 1010
PkrI GCNGC 4 cut(s) 64, 141, 1111, 1114
PleI GAGTC 3 cut(s) 106, 734, 1043
PpsI GAGTC 3 cut(s) 106, 734, 1043
PpuMI RGGWCCY 1 cut(s) 158
PsiI TTATAA 2 cut(s) 602, 1067
Psp124BI GAGCTC 1 cut(s) 289
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 1 cut(s) 479
PspFI CCCAGC 1 cut(s) 1138
PspGI CCWGG 1 cut(s) 479
PspN4I GGNNCC 5 cut(s) 160, 431, 720, 1031, 1244
PspPI GGNCC 4 cut(s) 158, 854, 1003, 1030
PspPPI RGGWCCY 1 cut(s) 158
PsuI RGATCY 2 cut(s) 748, 1072
RsaI GTAC 5 cut(s) 425, 431, 720, 861, 992
RsaNI GTAC 5 cut(s) 424, 430, 719, 860, 991
RseI CAYNNNNRTG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 289
SaqAI TTAA 3 cut(s) 195, 462, 633
SatI GCNGC 4 cut(s) 63, 140, 1110, 1113
Sau3AI GATC 7 cut(s) 530, 568, 651, 748, 760, 1072, 1147
Sau96I GGNCC 4 cut(s) 158, 854, 1003, 1030
SchI GAGTC 3 cut(s) 107, 734, 1044
ScrFI CCNGG 2 cut(s) 212, 481
SduI GDGCHC 4 cut(s) 289, 549, 712, 960
SfaNI GCATC 3 cut(s) 23, 1072, 1215
SinI GGWCC 2 cut(s) 158, 854
SmiMI CAYNNNNRTG 1 cut(s) 398
Sse9I AATT 5 cut(s) 266, 459, 509, 604, 1024
SsiI CCGC 3 cut(s) 65, 119, 421
SspMI CTAG 4 cut(s) 231, 807, 929, 1196
SstI GAGCTC 1 cut(s) 289
StyD4I CCNGG 2 cut(s) 210, 479
StyI CCWWGG 3 cut(s) 230, 712, 1045
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 1 cut(s) 622
TaqI TCGA 2 cut(s) 283, 1008
TasI AATT 5 cut(s) 266, 459, 509, 604, 1024
TatI WGTACW 2 cut(s) 859, 990
TfiI GAWTC 4 cut(s) 148, 383, 813, 1010
Tru1I TTAA 3 cut(s) 195, 462, 633
Tru9I TTAA 3 cut(s) 195, 462, 633
TscAI CASTG 6 cut(s) 264, 372, 445, 712, 886, 1110
TseI GCWGC 4 cut(s) 62, 139, 1109, 1112
TspDTI ATGAA 8 cut(s) 443, 597, 806, 837, 900, 1050, 1179, 1192
TspRI CASTG 6 cut(s) 264, 372, 445, 712, 886, 1110
VpaK11BI GGWCC 2 cut(s) 158, 854
XapI RAATTY 1 cut(s) 604
XceI RCATGY 1 cut(s) 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 848
XmaJI CCTAGG 1 cut(s) 230
XmnI GAANNNNTTC 2 cut(s) 450, 557
XspI CTAG 4 cut(s) 231, 807, 929, 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.