Rw6G043090

Catalyzes the ferrous insertion into protoporphyrin IX

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004323
Physical Location & Seq
Forward (+)
66477106 .. 66479897
2792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043090.1

Sequence Viewer

Length: 1458 bp
ATGGACGCCGCCGTTTCATGCTCCGGTGTCCTTCCCCAAATGAACCTCTCCGGCTCAGATCTTAAATTTCCGATGGGTAGGTCTTCGTGCTCTTACGCGAGGTCAATTGGGGCGGTTTCTTGCAATGAAGGACTGCGAATTGTTCCGAAAAAGCTTGAATTCTTGTGCTCTGCTGCGTCGAGACATGAAGAGAGGAATGAAATGGTGTTTGGGGCATCGCATTGCGGGAAGAAAAGCCCTGTTGGGGAGGCATTGTGTGTTGCTGCGGGTGTATGCACTTATGGTGGGACTGCTGTGGAGTCTCATCACTCCCACATTGCGGAAGAGAATGTTGGAGTGCTGCTCCTGAATCTTGGAGGACCCGAGACGCTTGATGATGTTCAGCCATTTCTGTTTAATCTATTCGCTGACCCGGATATCATACGTCTACCTAGGGCATTTCGGTTTCTTCAGAAGCCACTGGCAAAATTGATTTCTGTGCTTCGAGCTCCTAAGAGCAAAGAAGGGTATGCGTCTATTGGAGGTGGTTCGCCCTTGCGTAAGATAACAGATGAGCAGGCAAATGCACTGAAAATGGCTTTGGAATCAAAGGACATGCCGGTGAATGTCTATGTGGGAATGCGGTACTGGTACCCATTCACTGAGGAAGCTGTTCATCAAATTAAGAGGGACAGAATAACCAGGCTTGTTGTCCTGCCTCTATATCCTCAATTCTCTATCTCTACAACTGGATCAAGCGTTCGTGTGCTCCAGAAGATTTTCAGGAACGATCCATATCTATCAAGGTTGCCTGTTTCAGTTATAAATTCATGGTATCAACGTGAAGGTTATATTAAGTCAATGGCTGACTTGATCTCAAAAGAGCTAGAGAGTTTTTCTGAGCCTGCACAGGTCATGATATTCTTCAGTGCCCACGGGGTACCACTCAGTTATGTGAAGGACTCTGGAGATCCATACAAAGATCAAATGGAGGAGTGTATCTTTCTAATAATGAAAGAGTTGAAAGCTAGAGGAATCAATAATGAACATACTCTTGCCTACCAGAGCCGAGTTGGTCCTGTACAATGGTTGAAGCCATACACTGATGAAGTTCTTGTTGAGCTTGGCCAGAAAGGAGTAAAAAGTCTTCTAGCTGTTCCAGTCAGCTTTGTGAGTGAGCACATAGAAACTCTTGAAGAGATAGATATGGAGTACAAGGAGTTGGCCCTCGAATCCGGCATTGAGAATTGGGGCCGAGTCCCTGCCCTTGGCCTTACCCCTTCATTTATAACAGATCTGGCAGATGCAGTTATAGAGGCTCTACCACTGGCAGCAGCCACTTCAACCCCAAAGAATACCCCAGCAGTTGATCATGACCCTATGAGATGGGCTATCAAAATGTTCTTTGGTTCATTTCTAGCATTCATTTTGTTCTTTTCACCAAAGATGCTAATGGCATTCAGGAACCACCTGATTTGA

Protein Analysis

485

Amino Acids

53.79

Weight (kDa)

6.29

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ferrochelatase PF00762 111 - 433 2.7e-111 Ferrochelatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 803, 1268
Acc65I GGTACC 2 cut(s) 630, 919
AccB1I GGYRCC 2 cut(s) 630, 919
AccI GTMKAC 1 cut(s) 427
AccII CGCG 1 cut(s) 98
AciI CCGC 6 cut(s) 9, 113, 225, 266, 320, 622
AclWI GGATC 3 cut(s) 739, 764, 944
AcoI YGGCCR 1 cut(s) 1105
AcsI RAATTY 3 cut(s) 65, 158, 805
AcuI CTGAAG 2 cut(s) 434, 889
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 5 cut(s) 626, 632, 921, 1062, 1193
AfiI CCNNNNNNNGG 3 cut(s) 244, 319, 1247
AgsI TTSAA 5 cut(s) 158, 1003, 1072, 1175, 1323
AjnI CCWGG 1 cut(s) 680
AjuI GAANNNNNNNTTGG 6 cut(s) 192, 224, 315, 347, 563, 595
AloI GAACNNNNNNTCC 2 cut(s) 723, 755
AluBI AGCT 8 cut(s) 154, 488, 650, 865, 1007, 1102, 1133, 1146
AluI AGCT 8 cut(s) 154, 488, 650, 865, 1007, 1102, 1133, 1146
Alw21I GWGCWC 5 cut(s) 92, 170, 490, 750, 1161
Alw26I GTCTC 3 cut(s) 175, 306, 359
AlwI GGATC 3 cut(s) 739, 764, 944
Ama87I CYCGRG 1 cut(s) 362
AoxI GGCC 4 cut(s) 1105, 1203, 1231, 1249
ApeKI GCWGC 5 cut(s) 173, 263, 340, 1310, 1313
ApoI RAATTY 3 cut(s) 65, 158, 805
Asp700I GAANNNNTTC 2 cut(s) 651, 758
Asp718I GGTACC 2 cut(s) 630, 919
AspA2I CCTAGG 1 cut(s) 431
AspS9I GGNCC 4 cut(s) 359, 1055, 1204, 1231
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 2 cut(s) 613, 1410
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 2 cut(s) 359, 1055
AvrII CCTAGG 1 cut(s) 431
BaeGI GKGCMC 1 cut(s) 913
BalI TGGCCA 1 cut(s) 1107
BanI GGYRCC 2 cut(s) 630, 919
BanII GRGCYC 1 cut(s) 490
BarI GAAGNNNNNNTAC 2 cut(s) 1110, 1142
BbsI GAAGAC 2 cut(s) 75, 1118
Bbv12I GWGCWC 5 cut(s) 92, 170, 490, 750, 1161
BbvI GCAGC 5 cut(s) 160, 250, 327, 1322, 1325
BccI CCATC 2 cut(s) 67, 1359
BciT130I CCWGG 1 cut(s) 682
BclI TGATCA 1 cut(s) 1348
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 3 cut(s) 175, 306, 359
BfaI CTAG 5 cut(s) 432, 866, 1008, 1130, 1397
BglII AGATCT 2 cut(s) 58, 1273
BisI GCNGC 6 cut(s) 9, 174, 264, 341, 1311, 1314
BlnI CCTAGG 1 cut(s) 431
BlsI GCNGC 6 cut(s) 10, 175, 265, 342, 1312, 1315
Bme1390I CCNGG 2 cut(s) 413, 682
Bme18I GGWCC 2 cut(s) 359, 1055
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 4 cut(s) 359, 1055, 1204, 1231
BmiI GGNNCC 5 cut(s) 361, 632, 921, 1232, 1445
BmrFI CCNGG 2 cut(s) 413, 682
BmsI GCATC 3 cut(s) 224, 1273, 1416
BpiI GAAGAC 2 cut(s) 75, 1118
BplI GAGNNNNNCTC 2 cut(s) 327, 359
BpmI CTGGAG 2 cut(s) 734, 966
BpuMI CCSGG 1 cut(s) 413
BsaBI GATNNNNATC 1 cut(s) 774
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 3 cut(s) 431, 913, 1246
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 319, 1247
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 5 cut(s) 465, 632, 733, 1139, 1311
Bse3DI GCAATG 3 cut(s) 130, 220, 315
Bse8I GATNNNNATC 1 cut(s) 774
BseBI CCWGG 1 cut(s) 682
BseDI CCNNGG 3 cut(s) 431, 913, 1246
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 3 cut(s) 244, 319, 1247
BseMI GCAATG 3 cut(s) 130, 220, 315
BseMII CTCAG 4 cut(s) 69, 633, 870, 940
BseNI ACTGG 5 cut(s) 465, 632, 733, 1139, 1311
BseRI GAGGAG 1 cut(s) 986
BseSI GKGCMC 1 cut(s) 913
BseXI GCAGC 5 cut(s) 160, 250, 327, 1322, 1325
BseYI CCCAGC 1 cut(s) 1339
BsgI GTGCAG 1 cut(s) 870
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 4 cut(s) 1107, 1205, 1233, 1251
BshNI GGYRCC 2 cut(s) 630, 919
BsiHKAI GWGCWC 5 cut(s) 92, 170, 490, 750, 1161
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 5 cut(s) 24, 51, 413, 599, 1215
BslFI GGGAC 3 cut(s) 301, 683, 1223
BslI CCNNNNNNNGG 3 cut(s) 244, 319, 1247
BsmAI GTCTC 3 cut(s) 175, 306, 359
BsmBI CGTCTC 1 cut(s) 359
BsmFI GGGAC 3 cut(s) 301, 683, 1223
BsmI GAATGC 3 cut(s) 624, 1400, 1436
BsnI GGCC 4 cut(s) 1107, 1205, 1233, 1251
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 6 cut(s) 92, 170, 490, 750, 913, 1161
Bsp1407I TGTACA 1 cut(s) 1060
Bsp143I GATC 8 cut(s) 58, 731, 769, 852, 949, 961, 1273, 1348
BspACI CCGC 6 cut(s) 9, 113, 225, 266, 320, 622
BspANI GGCC 4 cut(s) 1107, 1205, 1233, 1251
BspCNI CTCAG 4 cut(s) 68, 634, 871, 939
BspFNI CGCG 1 cut(s) 98
BspHI TCATGA 2 cut(s) 894, 1351
BspLI GGNNCC 5 cut(s) 361, 632, 921, 1232, 1445
BspPI GGATC 3 cut(s) 739, 764, 944
BspT107I GGYRCC 2 cut(s) 630, 919
BsrDI GCAATG 3 cut(s) 130, 220, 315
BsrFI RCCGGY 1 cut(s) 598
BsrGI TGTACA 1 cut(s) 1060
BsrI ACTGG 5 cut(s) 465, 632, 733, 1139, 1311
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 3 cut(s) 431, 913, 1246
BssMI GATC 8 cut(s) 58, 731, 769, 852, 949, 961, 1273, 1348
BssNI GRCGYC 1 cut(s) 6
BssT1I CCWWGG 2 cut(s) 431, 1246
Bst2UI CCWGG 1 cut(s) 682
Bst6I CTCTTC 3 cut(s) 183, 318, 1170
BstACI GRCGYC 1 cut(s) 6
BstAUI TGTACA 1 cut(s) 1060
BstC8I GCNNGC 2 cut(s) 558, 885
BstDEI CTNAG 5 cut(s) 55, 492, 642, 879, 926
BstDSI CCRYGG 1 cut(s) 913
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 8 cut(s) 61, 734, 772, 855, 952, 964, 1276, 1351
BstMAI GTCTC 3 cut(s) 175, 306, 359
BstMBI GATC 8 cut(s) 58, 731, 769, 852, 949, 961, 1273, 1348
BstNI CCWGG 1 cut(s) 682
BstNSI RCATGY 1 cut(s) 598
BstSCI CCNGG 2 cut(s) 411, 680
BstSLI GKGCMC 1 cut(s) 913
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 5 cut(s) 160, 250, 327, 1322, 1325
BstV2I GAAGAC 2 cut(s) 75, 1118
BstX2I RGATCY 3 cut(s) 58, 949, 1273
BstYI RGATCY 3 cut(s) 58, 949, 1273
BsuRI GGCC 4 cut(s) 1107, 1205, 1233, 1251
BtgI CCRYGG 1 cut(s) 913
BtgZI GCGATG 1 cut(s) 201
BtsIMutI CAGTG 6 cut(s) 458, 566, 639, 913, 1080, 1304
Cac8I GCNNGC 2 cut(s) 558, 885
CciI TCATGA 2 cut(s) 894, 1351
Cfr10I RCCGGY 1 cut(s) 598
Cfr13I GGNCC 4 cut(s) 359, 1055, 1204, 1231
CseI GACGC 4 cut(s) 14, 165, 376, 501
Csp6I GTAC 5 cut(s) 625, 631, 920, 1061, 1192
CspCI CAANNNNNGTGG 1 cut(s) 1436
CviAII CATG 6 cut(s) 18, 185, 595, 810, 895, 1352
CviQI GTAC 5 cut(s) 625, 631, 920, 1061, 1192
DdeI CTNAG 5 cut(s) 55, 492, 642, 879, 926
DpnI GATC 8 cut(s) 60, 733, 771, 854, 951, 963, 1275, 1350
DpnII GATC 8 cut(s) 58, 731, 769, 852, 949, 961, 1273, 1348
EaeI YGGCCR 1 cut(s) 1105
Eam1104I CTCTTC 3 cut(s) 183, 318, 1170
EarI CTCTTC 3 cut(s) 183, 318, 1170
Ecl136II GAGCTC 1 cut(s) 488
Eco130I CCWWGG 2 cut(s) 431, 1246
Eco24I GRGCYC 1 cut(s) 490
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 2 cut(s) 359, 1055
Eco53kI GAGCTC 1 cut(s) 488
Eco57I CTGAAG 2 cut(s) 434, 889
Eco88I CYCGRG 1 cut(s) 362
EcoICRI GAGCTC 1 cut(s) 488
EcoO109I RGGNCCY 1 cut(s) 359
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 680
EcoRV GATATC 1 cut(s) 418
EcoT14I CCWWGG 2 cut(s) 431, 1246
EcoT38I GRGCYC 1 cut(s) 490
ErhI CCWWGG 2 cut(s) 431, 1246
Esp3I CGTCTC 1 cut(s) 359
FaeI CATG 6 cut(s) 21, 188, 598, 813, 898, 1355
FaqI GGGAC 3 cut(s) 301, 683, 1223
FatI CATG 6 cut(s) 17, 184, 594, 809, 894, 1351
FauI CCCGC 2 cut(s) 218, 259
FbaI TGATCA 1 cut(s) 1348
FblI GTMKAC 1 cut(s) 427
Fnu4HI GCNGC 6 cut(s) 9, 174, 264, 341, 1311, 1314
FriOI GRGCYC 1 cut(s) 490
Fsp4HI GCNGC 6 cut(s) 9, 174, 264, 341, 1311, 1314
FspBI CTAG 5 cut(s) 432, 866, 1008, 1130, 1397
GluI GCNGC 6 cut(s) 9, 174, 264, 341, 1311, 1314
GsaI CCCAGC 1 cut(s) 1343
GsuI CTGGAG 2 cut(s) 734, 966
HaeIII GGCC 4 cut(s) 1107, 1205, 1233, 1251
HapII CCGG 5 cut(s) 24, 51, 413, 599, 1215
HgaI GACGC 4 cut(s) 14, 165, 376, 501
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 6 cut(s) 21, 188, 598, 813, 898, 1355
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 7 cut(s) 299, 349, 584, 941, 1014, 1211, 1236
HpaII CCGG 5 cut(s) 24, 51, 413, 599, 1215
HphI GGTGA 2 cut(s) 613, 1410
Hpy166II GTNNAC 1 cut(s) 428
Hpy188I TCNGA 5 cut(s) 58, 72, 147, 453, 880
Hpy188III TCNNGA 9 cut(s) 180, 346, 751, 763, 895, 945, 1172, 1352, 1441
Hpy8I GTNNAC 1 cut(s) 428
Hpy99I CGWCG 1 cut(s) 181
HpyAV CCTTC 6 cut(s) 41, 122, 497, 818, 931, 1269
HpyCH4IV ACGT 2 cut(s) 424, 820
HpyCH4V TGCA 5 cut(s) 123, 276, 566, 887, 1286
HpyF3I CTNAG 5 cut(s) 55, 492, 642, 879, 926
HpySE526I ACGT 2 cut(s) 424, 820
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 6 cut(s) 21, 188, 598, 813, 898, 1355
KpnI GGTACC 2 cut(s) 634, 923
Ksp22I TGATCA 1 cut(s) 1348
Kzo9I GATC 8 cut(s) 58, 731, 769, 852, 949, 961, 1273, 1348
LmnI GCTCC 4 cut(s) 26, 348, 493, 753
Lsp1109I GCAGC 5 cut(s) 160, 250, 327, 1322, 1325
LweI GCATC 3 cut(s) 224, 1273, 1416
MaeI CTAG 5 cut(s) 432, 866, 1008, 1130, 1397
MaeII ACGT 2 cut(s) 424, 820
MalI GATC 8 cut(s) 60, 733, 771, 854, 951, 963, 1275, 1350
MboI GATC 8 cut(s) 58, 731, 769, 852, 949, 961, 1273, 1348
MboII GAAGA 9 cut(s) 75, 200, 241, 335, 440, 766, 895, 1118, 1187
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 3 cut(s) 58, 949, 1273
MhlI GDGCHC 6 cut(s) 92, 170, 490, 750, 913, 1161
MlsI TGGCCA 1 cut(s) 1107
MluCI AATT 9 cut(s) 65, 105, 138, 158, 467, 660, 710, 805, 1225
MluNI TGGCCA 1 cut(s) 1107
MlyI GAGTC 3 cut(s) 308, 935, 1245
MmeI TCCRAC 1 cut(s) 313
Mox20I TGGCCA 1 cut(s) 1107
MroXI GAANNNNTTC 2 cut(s) 651, 758
MscI TGGCCA 1 cut(s) 1107
MseI TTAA 4 cut(s) 63, 396, 663, 834
MslI CAYNNNNRTG 1 cut(s) 599
Msp20I TGGCCA 1 cut(s) 1107
MspI CCGG 5 cut(s) 24, 51, 413, 599, 1215
MspR9I CCNGG 2 cut(s) 413, 682
MunI CAATTG 1 cut(s) 105
Mva1269I GAATGC 3 cut(s) 624, 1400, 1436
MvaI CCWGG 1 cut(s) 682
MvnI CGCG 1 cut(s) 98
NciI CCSGG 1 cut(s) 413
NdeII GATC 8 cut(s) 58, 731, 769, 852, 949, 961, 1273, 1348
NlaIII CATG 6 cut(s) 21, 188, 598, 813, 898, 1355
NlaIV GGNNCC 5 cut(s) 361, 632, 921, 1232, 1445
NmeAIII GCCGAG 2 cut(s) 1073, 1259
NspI RCATGY 1 cut(s) 598
PagI TCATGA 2 cut(s) 894, 1351
PctI GAATGC 3 cut(s) 624, 1400, 1436
PdmI GAANNNNTTC 2 cut(s) 651, 758
PfeI GAWTC 4 cut(s) 349, 584, 1014, 1211
PkrI GCNGC 6 cut(s) 10, 175, 265, 342, 1312, 1315
PleI GAGTC 3 cut(s) 307, 935, 1244
PpsI GAGTC 3 cut(s) 307, 935, 1244
PpuMI RGGWCCY 1 cut(s) 359
PsiI TTATAA 2 cut(s) 803, 1268
Psp124BI GAGCTC 1 cut(s) 490
Psp5II RGGWCCY 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 680
PspFI CCCAGC 1 cut(s) 1339
PspGI CCWGG 1 cut(s) 680
PspN4I GGNNCC 5 cut(s) 361, 632, 921, 1232, 1445
PspPI GGNCC 4 cut(s) 359, 1055, 1204, 1231
PspPPI RGGWCCY 1 cut(s) 359
PsuI RGATCY 3 cut(s) 58, 949, 1273
RsaI GTAC 5 cut(s) 626, 632, 921, 1062, 1193
RsaNI GTAC 5 cut(s) 625, 631, 920, 1061, 1192
RseI CAYNNNNRTG 1 cut(s) 599
SacI GAGCTC 1 cut(s) 490
SaqAI TTAA 4 cut(s) 63, 396, 663, 834
SatI GCNGC 6 cut(s) 9, 174, 264, 341, 1311, 1314
Sau3AI GATC 8 cut(s) 58, 731, 769, 852, 949, 961, 1273, 1348
Sau96I GGNCC 4 cut(s) 359, 1055, 1204, 1231
SchI GAGTC 3 cut(s) 308, 935, 1245
ScrFI CCNGG 2 cut(s) 413, 682
SduI GDGCHC 6 cut(s) 92, 170, 490, 750, 913, 1161
SfaNI GCATC 3 cut(s) 224, 1273, 1416
SinI GGWCC 2 cut(s) 359, 1055
SmiMI CAYNNNNRTG 1 cut(s) 599
Sse9I AATT 9 cut(s) 65, 105, 138, 158, 467, 660, 710, 805, 1225
SsiI CCGC 6 cut(s) 9, 113, 225, 266, 320, 622
SspMI CTAG 5 cut(s) 432, 866, 1008, 1130, 1397
SstI GAGCTC 1 cut(s) 490
StyD4I CCNGG 2 cut(s) 411, 680
StyI CCWWGG 2 cut(s) 431, 1246
TaiI ACGT 2 cut(s) 427, 823
TaqI TCGA 3 cut(s) 179, 484, 1209
TasI AATT 9 cut(s) 65, 105, 138, 158, 467, 660, 710, 805, 1225
TatI WGTACW 2 cut(s) 1060, 1191
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 4 cut(s) 349, 584, 1014, 1211
Tru1I TTAA 4 cut(s) 63, 396, 663, 834
Tru9I TTAA 4 cut(s) 63, 396, 663, 834
TscAI CASTG 6 cut(s) 465, 573, 646, 913, 1087, 1311
TseI GCWGC 5 cut(s) 173, 263, 340, 1310, 1313
TspRI CASTG 6 cut(s) 465, 573, 646, 913, 1087, 1311
VpaK11BI GGWCC 2 cut(s) 359, 1055
XapI RAATTY 3 cut(s) 65, 158, 805
XceI RCATGY 1 cut(s) 598
XcmI CCANNNNNNNNNTGG 1 cut(s) 1049
XmaJI CCTAGG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 427
XmnI GAANNNNTTC 2 cut(s) 651, 758
XspI CTAG 5 cut(s) 432, 866, 1008, 1130, 1397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.