RchiOBHm_Chr7g0191621

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0011283
Physical Location & Seq
Forward (+)
10332827 .. 10334191
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17125

Sequence Viewer

Length: 612 bp
ATGTTTCTCTGTGCTGGCACAAGGTGTTCAGTCAAATTGCTTCCCTCATACCAGCACCACCCCGAACGATGGCCTCCAACTCCAAGTAGCCGCCGTGGACCCAACATCGAGTCCTGGGTCGCCCCCAGCAACACCACCTGCCCAGTCACCCGAACCCAACTTGCCGACTTCACCCTCATCCCCAACCACACTCTCCGCCGGCTCATTCAGGAGTGGTACGTCGCCAACCGCTCTTTCGGCGTGGTTCAAATTCCGACGCCGAAACGACTCGTCGACCCGACCAGAACTGAACAGCGAGGCGCTCATGCTTATGGTAATTTTCCCCCTCGTAGAGTCCGACAGCGATGTATTGATCTAGAACCTCCTGGCCGGGGTGTGATCGAAATTCTCCAGAACCCAATCGCTCATCCGCGGGCTTTGAAAATCGGAATCAAAGCCTTGTTCGTGTTGTGCTTGGTGAAGCAAATGCGTAAGAAAGTCGTGTCGGCCGGAGCACCGGAGATGCTGATCAACACTCTCGCCGACTTCAAGAAATGCGACTCGGAGAGAGCCTTGGCCACCATAGAGAGCTCCTCTGCGAAGAGTAGAAGGAGTCTTGCACAATCCCATTGA

Protein Analysis

203

Amino Acids

22.84

Weight (kDa)

10.45

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 125 - 190 9.4e-11 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 146
AccBSI CCGCTC 1 cut(s) 231
AccI GTMKAC 1 cut(s) 273
AccII CGCG 1 cut(s) 412
AciI CCGC 5 cut(s) 91, 196, 229, 410, 412
AcoI YGGCCR 3 cut(s) 367, 486, 555
AcsI RAATTY 2 cut(s) 249, 384
AcyI GRCGYC 1 cut(s) 257
AdeI CACNNNGTG 1 cut(s) 24
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 2 cut(s) 69, 371
AgsI TTSAA 3 cut(s) 248, 421, 529
AjnI CCWGG 2 cut(s) 113, 364
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 1 cut(s) 570
AluI AGCT 1 cut(s) 570
Alw21I GWGCWC 2 cut(s) 496, 572
AoxI GGCC 4 cut(s) 71, 367, 486, 555
ApoI RAATTY 2 cut(s) 249, 384
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 1 cut(s) 98
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 3 cut(s) 139, 163, 469
AvaII GGWCC 1 cut(s) 98
BalI TGGCCA 1 cut(s) 557
BanII GRGCYC 1 cut(s) 572
Bbv12I GWGCWC 2 cut(s) 496, 572
BccI CCATC 1 cut(s) 63
BceAI ACGGC 1 cut(s) 78
BciT130I CCWGG 2 cut(s) 115, 366
BclI TGATCA 1 cut(s) 507
BcnI CCSGG 1 cut(s) 371
BfaI CTAG 1 cut(s) 356
BfoI RGCGCY 1 cut(s) 303
BfuAI ACCTGC 1 cut(s) 146
BisI GCNGC 1 cut(s) 91
BlsI GCNGC 1 cut(s) 92
Bme1390I CCNGG 3 cut(s) 115, 366, 371
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 3 cut(s) 115, 366, 371
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 1 cut(s) 492
BmuI ACTGGG 1 cut(s) 137
BplI GAGNNNNNCTC 2 cut(s) 557, 589
BpmI CTGGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 371
BsaBI GATNNNNATC 1 cut(s) 506
BsaHI GRCGYC 1 cut(s) 257
BsaJI CCNNGG 5 cut(s) 94, 114, 370, 410, 552
BsaWI WCCGGW 1 cut(s) 496
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 371
Bse118I RCCGGY 1 cut(s) 198
Bse1I ACTGG 1 cut(s) 143
Bse8I GATNNNNATC 1 cut(s) 506
BseBI CCWGG 2 cut(s) 115, 366
BseDI CCNNGG 5 cut(s) 94, 114, 370, 410, 552
BseGI GGATG 2 cut(s) 177, 406
BseJI GATNNNNATC 1 cut(s) 506
BseLI CCNNNNNNNGG 2 cut(s) 69, 371
BseNI ACTGG 1 cut(s) 143
BseRI GAGGAG 1 cut(s) 562
BseX3I CGGCCG 1 cut(s) 486
BseYI CCCAGC 1 cut(s) 125
Bsh1236I CGCG 1 cut(s) 412
Bsh1285I CGRYCG 1 cut(s) 489
BshFI GGCC 4 cut(s) 73, 369, 488, 557
BsiEI CGRYCG 1 cut(s) 489
BsiHKAI GWGCWC 2 cut(s) 496, 572
BsiSI CCGG 4 cut(s) 199, 370, 489, 497
BslI CCNNNNNNNGG 2 cut(s) 69, 371
BsnI GGCC 4 cut(s) 73, 369, 488, 557
Bsp1286I GDGCHC 2 cut(s) 496, 572
Bsp143I GATC 3 cut(s) 352, 378, 507
BspACI CCGC 5 cut(s) 91, 196, 229, 410, 412
BspANI GGCC 4 cut(s) 73, 369, 488, 557
BspFNI CGCG 1 cut(s) 412
BspLI GGNNCC 1 cut(s) 100
BspMI ACCTGC 1 cut(s) 146
BsrBI CCGCTC 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 198
BsrI ACTGG 1 cut(s) 143
BssAI RCCGGY 1 cut(s) 198
BssECI CCNNGG 5 cut(s) 94, 114, 370, 410, 552
BssMI GATC 3 cut(s) 352, 378, 507
BssNI GRCGYC 1 cut(s) 257
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 115, 366
Bst6I CTCTTC 1 cut(s) 575
BstACI GRCGYC 1 cut(s) 257
BstC8I GCNNGC 3 cut(s) 16, 200, 414
BstDSI CCRYGG 2 cut(s) 94, 410
BstF5I GGATG 2 cut(s) 177, 406
BstFNI CGCG 1 cut(s) 412
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 3 cut(s) 355, 381, 510
BstMBI GATC 3 cut(s) 352, 378, 507
BstMCI CGRYCG 1 cut(s) 489
BstMWI GCNNNNNNNGC 1 cut(s) 237
BstNI CCWGG 2 cut(s) 115, 366
BstSCI CCNGG 3 cut(s) 113, 364, 369
BstUI CGCG 1 cut(s) 412
BstZI CGGCCG 1 cut(s) 486
BsuRI GGCC 4 cut(s) 73, 369, 488, 557
BtgI CCRYGG 2 cut(s) 94, 410
BtgZI GCGATG 1 cut(s) 358
BtsCI GGATG 2 cut(s) 177, 406
BveI ACCTGC 1 cut(s) 146
Cac8I GCNNGC 3 cut(s) 16, 200, 414
CfoI GCGC 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 98
Cfr42I CCGCGG 1 cut(s) 413
CseI GACGC 1 cut(s) 265
Csp6I GTAC 1 cut(s) 217
CviAII CATG 1 cut(s) 305
CviQI GTAC 1 cut(s) 217
DpnI GATC 3 cut(s) 354, 380, 509
DpnII GATC 3 cut(s) 352, 378, 507
DraIII CACNNNGTG 1 cut(s) 24
EaeI YGGCCR 3 cut(s) 367, 486, 555
EagI CGGCCG 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 575
EarI CTCTTC 1 cut(s) 575
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 570
EclXI CGGCCG 1 cut(s) 486
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 1 cut(s) 572
Eco47I GGWCC 1 cut(s) 98
Eco52I CGGCCG 1 cut(s) 486
Eco53kI GAGCTC 1 cut(s) 570
EcoICRI GAGCTC 1 cut(s) 570
EcoRII CCWGG 2 cut(s) 113, 364
EcoT14I CCWWGG 1 cut(s) 552
EcoT38I GRGCYC 1 cut(s) 572
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 1 cut(s) 308
FaiI YATR 4 cut(s) 49, 306, 312, 563
FatI CATG 1 cut(s) 304
FauI CCCGC 1 cut(s) 405
FbaI TGATCA 1 cut(s) 507
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 91
FokI GGATG 2 cut(s) 164, 393
FriOI GRGCYC 1 cut(s) 572
Fsp4HI GCNGC 1 cut(s) 91
FspBI CTAG 1 cut(s) 356
GlaI GCGC 1 cut(s) 301
GluI GCNGC 1 cut(s) 91
GsaI CCCAGC 1 cut(s) 129
GsuI CTGGAG 1 cut(s) 374
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 4 cut(s) 73, 369, 488, 557
HapII CCGG 4 cut(s) 199, 370, 489, 497
HgaI GACGC 1 cut(s) 265
HhaI GCGC 1 cut(s) 302
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 1 cut(s) 308
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 6 cut(s) 110, 267, 333, 429, 539, 592
HpaII CCGG 4 cut(s) 199, 370, 489, 497
HphI GGTGA 3 cut(s) 139, 163, 469
Hpy166II GTNNAC 2 cut(s) 98, 274
Hpy188I TCNGA 4 cut(s) 255, 338, 428, 544
Hpy188III TCNNGA 4 cut(s) 209, 356, 391, 529
Hpy8I GTNNAC 2 cut(s) 98, 274
Hpy99I CGWCG 3 cut(s) 224, 259, 275
HpyAV CCTTC 1 cut(s) 582
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 1 cut(s) 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 237
HpySE526I ACGT 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 1 cut(s) 308
HspAI GCGC 1 cut(s) 300
KroI GCCGGC 1 cut(s) 198
KroNI GCCGGC 1 cut(s) 200
Ksp22I TGATCA 1 cut(s) 507
KspI CCGCGG 1 cut(s) 413
Kzo9I GATC 3 cut(s) 352, 378, 507
LmnI GCTCC 2 cut(s) 491, 575
LweI GCATC 1 cut(s) 492
MaeI CTAG 1 cut(s) 356
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 145
MalI GATC 3 cut(s) 354, 380, 509
MbiI CCGCTC 1 cut(s) 231
MboI GATC 3 cut(s) 352, 378, 507
MboII GAAGA 1 cut(s) 592
MhlI GDGCHC 2 cut(s) 496, 572
MlsI TGGCCA 1 cut(s) 557
MluCI AATT 4 cut(s) 35, 249, 316, 384
MluNI TGGCCA 1 cut(s) 557
MlyI GAGTC 5 cut(s) 119, 261, 342, 533, 601
MmeI TCCRAC 3 cut(s) 101, 278, 361
MnlI CCTC 7 cut(s) 55, 84, 185, 290, 336, 372, 583
Mox20I TGGCCA 1 cut(s) 557
MroNI GCCGGC 1 cut(s) 198
MscI TGGCCA 1 cut(s) 557
MslI CAYNNNNRTG 1 cut(s) 309
Msp20I TGGCCA 1 cut(s) 557
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 4 cut(s) 199, 370, 489, 497
MspR9I CCNGG 3 cut(s) 115, 366, 371
MvaI CCWGG 2 cut(s) 115, 366
MvnI CGCG 1 cut(s) 412
MwoI GCNNNNNNNGC 1 cut(s) 237
NaeI GCCGGC 1 cut(s) 200
NciI CCSGG 1 cut(s) 371
NdeII GATC 3 cut(s) 352, 378, 507
NgoMIV GCCGGC 1 cut(s) 198
NlaIII CATG 1 cut(s) 308
NlaIV GGNNCC 1 cut(s) 100
NmuCI GTSAC 1 cut(s) 145
PaqCI CACCTGC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 334
PdiI GCCGGC 1 cut(s) 200
PfeI GAWTC 1 cut(s) 429
PkrI GCNGC 1 cut(s) 92
PleI GAGTC 5 cut(s) 118, 261, 341, 533, 600
PpsI GAGTC 5 cut(s) 118, 261, 341, 533, 600
Psp124BI GAGCTC 1 cut(s) 572
Psp6I CCWGG 2 cut(s) 113, 364
PspFI CCCAGC 1 cut(s) 125
PspGI CCWGG 2 cut(s) 113, 364
PspN4I GGNNCC 1 cut(s) 100
PspPI GGNCC 1 cut(s) 98
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
RseI CAYNNNNRTG 1 cut(s) 309
SacI GAGCTC 1 cut(s) 572
SacII CCGCGG 1 cut(s) 413
SalI GTCGAC 1 cut(s) 272
SatI GCNGC 1 cut(s) 91
Sau3AI GATC 3 cut(s) 352, 378, 507
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 5 cut(s) 119, 261, 342, 533, 601
ScrFI CCNGG 3 cut(s) 115, 366, 371
SduI GDGCHC 2 cut(s) 496, 572
SetI ASST 5 cut(s) 26, 140, 222, 364, 572
SfaNI GCATC 1 cut(s) 492
Sfr303I CCGCGG 1 cut(s) 413
SgrBI CCGCGG 1 cut(s) 413
SinI GGWCC 1 cut(s) 98
SmiMI CAYNNNNRTG 1 cut(s) 309
Sse9I AATT 4 cut(s) 35, 249, 316, 384
SsiI CCGC 5 cut(s) 91, 196, 229, 410, 412
SspMI CTAG 1 cut(s) 356
SstI GAGCTC 1 cut(s) 572
StyD4I CCNGG 3 cut(s) 113, 364, 369
StyI CCWWGG 1 cut(s) 552
TaiI ACGT 1 cut(s) 222
TaqI TCGA 3 cut(s) 108, 273, 381
TasI AATT 4 cut(s) 35, 249, 316, 384
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 1 cut(s) 429
TseFI GTSAC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 98
XapI RAATTY 2 cut(s) 249, 384
XbaI TCTAGA 1 cut(s) 355
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.