RLG00000005798

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011283
Physical Location & Seq
Reverse (-)
1856208 .. 1856826
619 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005798

Sequence Viewer

Length: 537 bp
ATGGAGGTCCGAGTCAACTCGGCCTCGTTGATCGAGAACCTCCTGGCCAGGTTGCGAACAAAGGAGCTCCGAGCGCAAATAAGCAATGTGGACGAAATCCACCAAGGCGTGACCGAAATTCTCTGTAACCCAATCGCTCACCCGCGGGCTCTGGAAATCAGAATCAAAGCCTTGTTCGCCTTGTGCCTGGTGAAGCAAACACACGGCAAAGCAGTATCGGCCGAAGCGCCAGAGACGCTGATCTGCACCAAGATCTCCCTCGAGCTCGCCTCCCAACATCGGGTCACTCTTGGACGACGCTGGAGCTCTGATACCATTTGCTCTCTTCGGGATCAAAACCAGCCAGCTCCGCCGATCCTCATCCAACAGCAACGCCTACTACGTCGTTGTCTCTCCGGCGACGCCATCAGTTCGTCCTCGGGAAGTATGCGAAATCACGTGACAGGTAGATGGATCATATGGGACTTGGTCTATGAGGCATCCATCTCTTCATGTCTCACAGTCCAGGTCTGCAACCGACGAAGGGAGAAAAGGTAG

Protein Analysis

179

Amino Acids

20.04

Weight (kDa)

9.66

Isoelectric Point (pI)

59.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 2 - 81 7.7e-10 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 145
AciI CCGC 3 cut(s) 143, 145, 350
AclWI GGATC 3 cut(s) 339, 349, 461
AcoI YGGCCR 2 cut(s) 45, 219
AcsI RAATTY 1 cut(s) 117
AcvI CACGTG 1 cut(s) 439
AcyI GRCGYC 1 cut(s) 402
AfiI CCNNNNNNNGG 3 cut(s) 279, 280, 523
AjnI CCWGG 4 cut(s) 42, 47, 186, 504
AluBI AGCT 4 cut(s) 67, 265, 306, 347
AluI AGCT 4 cut(s) 67, 265, 306, 347
Alw21I GWGCWC 3 cut(s) 69, 267, 308
Alw26I GTCTC 3 cut(s) 227, 395, 500
AlwI GGATC 3 cut(s) 339, 349, 461
Ama87I CYCGRG 2 cut(s) 260, 418
AoxI GGCC 3 cut(s) 21, 45, 219
ApoI RAATTY 1 cut(s) 117
AspLEI GCGC 2 cut(s) 76, 229
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 131, 202
AvaI CYCGRG 2 cut(s) 260, 418
AvaII GGWCC 1 cut(s) 7
BalI TGGCCA 1 cut(s) 47
BanII GRGCYC 4 cut(s) 69, 151, 267, 308
BbrPI CACGTG 1 cut(s) 439
Bbv12I GWGCWC 3 cut(s) 69, 267, 308
BccI CCATC 3 cut(s) 413, 444, 491
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 4 cut(s) 44, 49, 188, 506
BcoDI GTCTC 3 cut(s) 227, 395, 500
BfoI RGCGCY 1 cut(s) 230
BglII AGATCT 1 cut(s) 252
Bme1390I CCNGG 4 cut(s) 44, 49, 188, 506
Bme18I GGWCC 1 cut(s) 7
BmeT110I CYCGRG 2 cut(s) 260, 418
BmgT120I GGNCC 1 cut(s) 7
BmrFI CCNGG 4 cut(s) 44, 49, 188, 506
BmsI GCATC 1 cut(s) 488
BplI GAGNNNNNCTC 2 cut(s) 254, 286
BpmI CTGGAG 1 cut(s) 322
BsaAI YACGTR 1 cut(s) 439
BsaBI GATNNNNATC 1 cut(s) 359
BsaHI GRCGYC 1 cut(s) 402
BsaJI CCNNGG 3 cut(s) 103, 143, 417
BsaXI ACNNNNNCTCC 1 cut(s) 518
Bsc4I CCNNNNNNNGG 3 cut(s) 279, 280, 523
Bse3DI GCAATG 1 cut(s) 91
Bse8I GATNNNNATC 1 cut(s) 359
BseBI CCWGG 4 cut(s) 44, 49, 188, 506
BseDI CCNNGG 3 cut(s) 103, 143, 417
BseGI GGATG 2 cut(s) 360, 479
BseJI GATNNNNATC 1 cut(s) 359
BseLI CCNNNNNNNGG 3 cut(s) 279, 280, 523
BseMI GCAATG 1 cut(s) 91
BseX3I CGGCCG 1 cut(s) 219
BsgI GTGCAG 1 cut(s) 229
Bsh1236I CGCG 1 cut(s) 145
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 3 cut(s) 23, 47, 221
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 3 cut(s) 69, 267, 308
BsiHKCI CYCGRG 2 cut(s) 260, 418
BsiSI CCGG 1 cut(s) 396
BslFI GGGAC 1 cut(s) 476
BslI CCNNNNNNNGG 3 cut(s) 279, 280, 523
BsmAI GTCTC 3 cut(s) 227, 395, 500
BsmBI CGTCTC 1 cut(s) 227
BsmFI GGGAC 1 cut(s) 476
BsnI GGCC 3 cut(s) 23, 47, 221
BsoBI CYCGRG 2 cut(s) 260, 418
Bsp1286I GDGCHC 4 cut(s) 69, 151, 267, 308
Bsp143I GATC 6 cut(s) 30, 240, 252, 331, 354, 453
BspACI CCGC 3 cut(s) 143, 145, 350
BspANI GGCC 3 cut(s) 23, 47, 221
BspFNI CGCG 1 cut(s) 145
BspPI GGATC 3 cut(s) 339, 349, 461
BsrDI GCAATG 1 cut(s) 91
BssECI CCNNGG 3 cut(s) 103, 143, 417
BssMI GATC 6 cut(s) 30, 240, 252, 331, 354, 453
BssNI GRCGYC 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 4 cut(s) 44, 49, 188, 506
Bst4CI ACNGT 1 cut(s) 502
Bst6I CTCTTC 2 cut(s) 330, 493
BstACI GRCGYC 1 cut(s) 402
BstBAI YACGTR 1 cut(s) 439
BstC8I GCNNGC 3 cut(s) 147, 267, 345
BstDSI CCRYGG 1 cut(s) 143
BstF5I GGATG 2 cut(s) 360, 479
BstFNI CGCG 1 cut(s) 145
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 2 cut(s) 76, 229
BstKTI GATC 6 cut(s) 33, 243, 255, 334, 357, 456
BstMAI GTCTC 3 cut(s) 227, 395, 500
BstMBI GATC 6 cut(s) 30, 240, 252, 331, 354, 453
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 5 cut(s) 73, 176, 218, 235, 349
BstNI CCWGG 4 cut(s) 44, 49, 188, 506
BstSCI CCNGG 4 cut(s) 42, 47, 186, 504
BstUI CGCG 1 cut(s) 145
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BstZI CGGCCG 1 cut(s) 219
BsuRI GGCC 3 cut(s) 23, 47, 221
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 2 cut(s) 360, 479
Cac8I GCNNGC 3 cut(s) 147, 267, 345
CfoI GCGC 2 cut(s) 76, 229
Cfr13I GGNCC 1 cut(s) 7
Cfr42I CCGCGG 1 cut(s) 146
CseI GACGC 3 cut(s) 244, 306, 410
CviAII CATG 1 cut(s) 492
DpnI GATC 6 cut(s) 32, 242, 254, 333, 356, 455
DpnII GATC 6 cut(s) 30, 240, 252, 331, 354, 453
EaeI YGGCCR 2 cut(s) 45, 219
EagI CGGCCG 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 330, 493
EarI CTCTTC 2 cut(s) 330, 493
EciI GGCGGA 1 cut(s) 339
Ecl136II GAGCTC 3 cut(s) 67, 265, 306
EclXI CGGCCG 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 103
Eco24I GRGCYC 4 cut(s) 69, 151, 267, 308
Eco47I GGWCC 1 cut(s) 7
Eco52I CGGCCG 1 cut(s) 219
Eco53kI GAGCTC 3 cut(s) 67, 265, 306
Eco72I CACGTG 1 cut(s) 439
Eco88I CYCGRG 2 cut(s) 260, 418
EcoICRI GAGCTC 3 cut(s) 67, 265, 306
EcoRII CCWGG 4 cut(s) 42, 47, 186, 504
EcoT14I CCWWGG 1 cut(s) 103
EcoT38I GRGCYC 4 cut(s) 69, 151, 267, 308
ErhI CCWWGG 1 cut(s) 103
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 1 cut(s) 495
FaiI YATR 5 cut(s) 428, 458, 460, 474, 493
FaqI GGGAC 1 cut(s) 476
FatI CATG 1 cut(s) 491
FauI CCCGC 2 cut(s) 138, 150
FauNDI CATATG 1 cut(s) 458
FokI GGATG 2 cut(s) 347, 466
FriOI GRGCYC 4 cut(s) 69, 151, 267, 308
GlaI GCGC 2 cut(s) 75, 228
GsuI CTGGAG 1 cut(s) 322
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 3 cut(s) 23, 47, 221
HapII CCGG 1 cut(s) 396
HgaI GACGC 3 cut(s) 244, 306, 410
HhaI GCGC 2 cut(s) 76, 229
Hin1I GRCGYC 1 cut(s) 402
Hin1II CATG 1 cut(s) 495
Hin6I GCGC 2 cut(s) 74, 227
HinP1I GCGC 2 cut(s) 74, 227
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 2 cut(s) 12, 162
HpaII CCGG 1 cut(s) 396
HphI GGTGA 2 cut(s) 131, 202
Hpy166II GTNNAC 2 cut(s) 16, 91
Hpy188I TCNGA 4 cut(s) 11, 71, 161, 310
Hpy188III TCNNGA 4 cut(s) 34, 152, 329, 420
Hpy8I GTNNAC 2 cut(s) 16, 91
Hpy99I CGWCG 4 cut(s) 300, 387, 404, 522
HpyAV CCTTC 1 cut(s) 516
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4IV ACGT 2 cut(s) 382, 438
HpyCH4V TGCA 2 cut(s) 246, 513
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 176, 218, 235, 349
HpySE526I ACGT 2 cut(s) 382, 438
Hsp92I GRCGYC 1 cut(s) 402
Hsp92II CATG 1 cut(s) 495
HspAI GCGC 2 cut(s) 74, 227
KspI CCGCGG 1 cut(s) 146
Kzo9I GATC 6 cut(s) 30, 240, 252, 331, 354, 453
LmnI GCTCC 4 cut(s) 64, 72, 303, 352
LweI GCATC 1 cut(s) 488
MaeII ACGT 2 cut(s) 382, 438
MaeIII GTNAC 4 cut(s) 109, 125, 283, 439
MalI GATC 6 cut(s) 32, 242, 254, 333, 356, 455
MboI GATC 6 cut(s) 30, 240, 252, 331, 354, 453
MboII GAAGA 2 cut(s) 317, 480
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 4 cut(s) 69, 151, 267, 308
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 1 cut(s) 117
MluNI TGGCCA 1 cut(s) 47
MlyI GAGTC 1 cut(s) 21
MmeI TCCRAC 1 cut(s) 388
MnlI CCTC 7 cut(s) 34, 50, 269, 280, 368, 427, 469
Mox20I TGGCCA 1 cut(s) 47
MscI TGGCCA 1 cut(s) 47
Msp20I TGGCCA 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 4 cut(s) 44, 49, 188, 506
MvaI CCWGG 4 cut(s) 44, 49, 188, 506
MvnI CGCG 1 cut(s) 145
MwoI GCNNNNNNNGC 5 cut(s) 73, 176, 218, 235, 349
NdeI CATATG 1 cut(s) 458
NdeII GATC 6 cut(s) 30, 240, 252, 331, 354, 453
NlaIII CATG 1 cut(s) 495
NmuCI GTSAC 3 cut(s) 109, 283, 439
PaeR7I CTCGAG 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 379
PfeI GAWTC 1 cut(s) 162
PflFI GACNNNGTC 1 cut(s) 467
PleI GAGTC 1 cut(s) 20
PmaCI CACGTG 1 cut(s) 439
PmlI CACGTG 1 cut(s) 439
PpsI GAGTC 1 cut(s) 20
Ppu21I YACGTR 1 cut(s) 439
Psp124BI GAGCTC 3 cut(s) 69, 267, 308
Psp6I CCWGG 4 cut(s) 42, 47, 186, 504
PspCI CACGTG 1 cut(s) 439
PspGI CCWGG 4 cut(s) 42, 47, 186, 504
PspPI GGNCC 1 cut(s) 7
PspXI VCTCGAGB 1 cut(s) 260
PsuI RGATCY 1 cut(s) 252
PsyI GACNNNGTC 1 cut(s) 467
SacI GAGCTC 3 cut(s) 69, 267, 308
SacII CCGCGG 1 cut(s) 146
Sau3AI GATC 6 cut(s) 30, 240, 252, 331, 354, 453
Sau96I GGNCC 1 cut(s) 7
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 4 cut(s) 44, 49, 188, 506
SduI GDGCHC 4 cut(s) 69, 151, 267, 308
SfaNI GCATC 1 cut(s) 488
Sfr274I CTCGAG 1 cut(s) 260
Sfr303I CCGCGG 1 cut(s) 146
SgrBI CCGCGG 1 cut(s) 146
SinI GGWCC 1 cut(s) 7
SlaI CTCGAG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 1 cut(s) 117
SsiI CCGC 3 cut(s) 143, 145, 350
SstI GAGCTC 3 cut(s) 69, 267, 308
StyD4I CCNGG 4 cut(s) 42, 47, 186, 504
StyI CCWWGG 1 cut(s) 103
TaaI ACNGT 1 cut(s) 502
TaiI ACGT 2 cut(s) 385, 441
TaqI TCGA 2 cut(s) 33, 261
TaqII GACCGA 1 cut(s) 128
TasI AATT 1 cut(s) 117
TfiI GAWTC 1 cut(s) 162
TseFI GTSAC 3 cut(s) 109, 283, 439
Tsp45I GTSAC 3 cut(s) 109, 283, 439
TspDTI ATGAA 1 cut(s) 480
Tth111I GACNNNGTC 1 cut(s) 467
VpaK11BI GGWCC 1 cut(s) 7
XapI RAATTY 1 cut(s) 117
XhoI CTCGAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.