RchiOBHm_Chr7g0198031

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014495
Physical Location & Seq
Forward (+)
15781002 .. 15784059
3058 bp
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UTR
Exon/CDS
Intron
PRQ17716

Sequence Viewer

Length: 828 bp
ATGAACCCGATAAGACGGGGGTGGGGTTGCAAAAAAGTCAGAAGCTTTAGCAGAGCTGAAAACGAAGAAGAAGAGAAAATGAAGCTCAAAGTATACGTGGATAGGATGTCACAACCTTCGCGTGCAATTTTGATCTTCTGCAAGGTCAATGGTATAGAATTCGAGGAGGTGCAAATCAACCTAGCCAAACAGCAGCAAAAGTCACCCGATTTCAAAAAAATAAACCCTATGGGACAAGTTCCCACTATTGCTGATGGGAGGTTTAATCTGTTTGAAAGCCATGCAATTCTTGTCTATCTTGCTTGTGCGTTTCCTGGAGTAGCAGATCACTGGTATCCTGCTGATCTTTTCAGGAGAGCTAAAATCAACTCAGTGTTGGATTGGCACCATTTTAACTTACGCCGTGGTGCAATGACATATGTTCTAAATACTGTACTTGCACCTGTGTTTGGACTTCGATTGAATCCACAAGCAGCTGCCGAAGCTGAGAAACTTCTGTCCTCCTCTTTGTCAAAAATTGAGTCTATTTGGCTCAAAGGGAATGGAAAATTCTTGTTGGGTGGCTTTCAACCATCCATAGCAGATCTCAGCCTGGTTTGTGAAATTATGCAACTTGAGCTTTTGGACGAGAACGACCGCAGTCGGATACTTGGCCCGCACAAGAAAGTTCTAGAGTGGATTGAGAATACAAAAAATGCGACAAGACCTCACTTTGAAGAAGTACATCAAATCCTCTACAGAGCCAAAACGAGATTTCAAGAGCAGCGGTCGATGGGAGGAAACAATACTAATTTGAGCAAAAGATTGGGTTTGCCCTCAAAGATGTGA

Protein Analysis

275

Amino Acids

31.5

Weight (kDa)

9.55

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 27 - 101 5.8e-15 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 33 - 106 9e-09 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 40 - 101 5.4e-10 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 156 - 228 1e-05 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41210
fragaria_vesca FvH4_5g02100
malus_domestica MD06G1081000.v1.1
prunus_persica Prupe.5G098100_v2.0.a1
pyrus_communis pycom06g07800
rosa_chinensis RchiOBHm_Chr7g0198031 RchiOBHm_Chr7g0198101
rosa_laevigata RLG00000003889 RLG00000003891
rosa_multiflora Rmu_sc0000221.1_g000031
rosa_roxburghii Rroxscaffold_3G00258000
rosa_rugosa Rorug07G0045600
rosa_samantha Rh7AG168900 Rh7BG172000 Rh7CG178500 Rh7DG171300
rosa_wichuraiana Rw7G014770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 384
AccI GTMKAC 1 cut(s) 93
AccII CGCG 1 cut(s) 121
AciI CCGC 3 cut(s) 637, 656, 766
AcsI RAATTY 2 cut(s) 158, 548
AfaI GTAC 2 cut(s) 435, 723
AfiI CCNNNNNNNGG 1 cut(s) 449
AgsI TTSAA 6 cut(s) 214, 275, 463, 569, 716, 758
AjnI CCWGG 2 cut(s) 313, 591
AluBI AGCT 7 cut(s) 45, 56, 85, 359, 476, 485, 619
AluI AGCT 7 cut(s) 45, 56, 85, 359, 476, 485, 619
AoxI GGCC 1 cut(s) 652
ApeKI GCWGC 4 cut(s) 193, 473, 476, 763
ApoI RAATTY 2 cut(s) 158, 548
ArsI GACNNNNNNTTYG 2 cut(s) 506, 538
AspS9I GGNCC 1 cut(s) 653
AsuHPI GGTGA 1 cut(s) 195
BanI GGYRCC 1 cut(s) 384
BbvI GCAGC 4 cut(s) 205, 463, 485, 775
BccI CCATC 3 cut(s) 248, 580, 766
BceAI ACGGC 1 cut(s) 387
BciT130I CCWGG 2 cut(s) 315, 593
BciVI GTATCC 2 cut(s) 345, 639
BfaI CTAG 2 cut(s) 182, 671
BfmI CTRYAG 1 cut(s) 736
BfuI GTATCC 2 cut(s) 345, 639
BglII AGATCT 1 cut(s) 583
BisI GCNGC 4 cut(s) 194, 474, 477, 764
BlsI GCNGC 4 cut(s) 195, 475, 478, 765
Bme1390I CCNGG 2 cut(s) 315, 593
BmgT120I GGNCC 1 cut(s) 653
BmiI GGNNCC 1 cut(s) 386
BmrFI CCNGG 2 cut(s) 315, 593
BoxI GACNNNNGTC 1 cut(s) 639
BpmI CTGGAG 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 635
BsaAI YACGTR 1 cut(s) 97
BsaJI CCNNGG 1 cut(s) 403
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse1I ACTGG 1 cut(s) 335
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 2 cut(s) 315, 593
BseDI CCNNGG 1 cut(s) 403
BseGI GGATG 2 cut(s) 111, 572
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 3 cut(s) 384, 477, 601
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 2 cut(s) 179, 493
BseXI GCAGC 4 cut(s) 205, 463, 485, 775
Bsh1236I CGCG 1 cut(s) 121
Bsh1285I CGRYCG 2 cut(s) 637, 770
BshFI GGCC 1 cut(s) 654
BshNI GGYRCC 1 cut(s) 384
BsiEI CGRYCG 2 cut(s) 637, 770
BslFI GGGAC 1 cut(s) 246
BslI CCNNNNNNNGG 1 cut(s) 449
BsmFI GGGAC 1 cut(s) 246
BsnI GGCC 1 cut(s) 654
Bsp143I GATC 4 cut(s) 132, 325, 343, 583
BspACI CCGC 3 cut(s) 637, 656, 766
BspANI GGCC 1 cut(s) 654
BspCNI CTCAG 3 cut(s) 383, 478, 600
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 384
BsrDI GCAATG 1 cut(s) 417
BsrI ACTGG 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 4 cut(s) 132, 325, 343, 583
BssNAI GTATAC 1 cut(s) 94
Bst1107I GTATAC 1 cut(s) 94
Bst2UI CCWGG 2 cut(s) 315, 593
Bst4CI ACNGT 1 cut(s) 433
Bst6I CTCTTC 1 cut(s) 66
BstBAI YACGTR 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 123, 656
BstDEI CTNAG 3 cut(s) 370, 486, 587
BstDSI CCRYGG 1 cut(s) 403
BstF5I GGATG 2 cut(s) 111, 572
BstFNI CGCG 1 cut(s) 121
BstKTI GATC 4 cut(s) 135, 328, 346, 586
BstMBI GATC 4 cut(s) 132, 325, 343, 583
BstMCI CGRYCG 2 cut(s) 637, 770
BstMWI GCNNNNNNNGC 2 cut(s) 482, 616
BstNI CCWGG 2 cut(s) 315, 593
BstPAI GACNNNNGTC 1 cut(s) 639
BstSCI CCNGG 2 cut(s) 313, 591
BstSFI CTRYAG 1 cut(s) 736
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 4 cut(s) 205, 463, 485, 775
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BstZ17I GTATAC 1 cut(s) 94
BsuI GTATCC 2 cut(s) 345, 639
BsuRI GGCC 1 cut(s) 654
BtgI CCRYGG 1 cut(s) 403
BtsCI GGATG 2 cut(s) 111, 572
BtsIMutI CAGTG 2 cut(s) 328, 378
Cac8I GCNNGC 2 cut(s) 123, 656
Cfr13I GGNCC 1 cut(s) 653
Csp6I GTAC 2 cut(s) 434, 722
CviAII CATG 1 cut(s) 281
CviQI GTAC 2 cut(s) 434, 722
DdeI CTNAG 3 cut(s) 370, 486, 587
DpnI GATC 4 cut(s) 134, 327, 345, 585
DpnII GATC 4 cut(s) 132, 325, 343, 583
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 313, 591
FaeI CATG 1 cut(s) 284
FaiI YATR 8 cut(s) 94, 155, 230, 282, 418, 420, 578, 608
FaqI GGGAC 1 cut(s) 246
FatI CATG 1 cut(s) 280
FauI CCCGC 1 cut(s) 663
FauNDI CATATG 1 cut(s) 418
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 4 cut(s) 194, 474, 477, 764
FokI GGATG 2 cut(s) 118, 559
Fsp4HI GCNGC 4 cut(s) 194, 474, 477, 764
FspBI CTAG 2 cut(s) 182, 671
GluI GCNGC 4 cut(s) 194, 474, 477, 764
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 1 cut(s) 654
Hin1II CATG 1 cut(s) 284
HindIII AAGCTT 1 cut(s) 43
HinfI GANTC 2 cut(s) 463, 521
HphI GGTGA 1 cut(s) 195
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 2 cut(s) 41, 645
Hpy188III TCNNGA 3 cut(s) 352, 671, 758
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 1 cut(s) 126
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 8 cut(s) 30, 125, 141, 172, 284, 410, 440, 610
HpyF10VI GCNNNNNNNGC 2 cut(s) 482, 616
HpyF3I CTNAG 3 cut(s) 370, 486, 587
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 1 cut(s) 284
Kzo9I GATC 4 cut(s) 132, 325, 343, 583
LpnPI CCDG 8 cut(s) 300, 316, 327, 337, 351, 456, 578, 605
Lsp1109I GCAGC 4 cut(s) 205, 463, 485, 775
MaeI CTAG 2 cut(s) 182, 671
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 108, 201
MalI GATC 4 cut(s) 134, 327, 345, 585
MboI GATC 4 cut(s) 132, 325, 343, 583
MboII GAAGA 5 cut(s) 77, 80, 83, 127, 728
MflI RGATCY 1 cut(s) 583
MluCI AATT 7 cut(s) 126, 158, 285, 516, 548, 603, 790
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 2 cut(s) 357, 623
MnlI CCTC 9 cut(s) 157, 160, 252, 511, 514, 717, 743, 770, 826
MseI TTAA 2 cut(s) 264, 393
MspA1I CMGCKG 2 cut(s) 476, 766
MspR9I CCNGG 2 cut(s) 315, 593
MvaI CCWGG 2 cut(s) 315, 593
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 2 cut(s) 482, 616
NdeI CATATG 1 cut(s) 418
NdeII GATC 4 cut(s) 132, 325, 343, 583
NlaIII CATG 1 cut(s) 284
NlaIV GGNNCC 1 cut(s) 386
NmuCI GTSAC 2 cut(s) 108, 201
PfeI GAWTC 1 cut(s) 463
PfoI TCCNGGA 1 cut(s) 313
PkrI GCNGC 4 cut(s) 195, 475, 478, 765
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 97
PshAI GACNNNNGTC 1 cut(s) 639
Psp6I CCWGG 2 cut(s) 313, 591
PspGI CCWGG 2 cut(s) 313, 591
PspN4I GGNNCC 1 cut(s) 386
PspPI GGNCC 1 cut(s) 653
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 2 cut(s) 435, 723
RsaNI GTAC 2 cut(s) 434, 722
SaqAI TTAA 2 cut(s) 264, 393
SatI GCNGC 4 cut(s) 194, 474, 477, 764
Sau3AI GATC 4 cut(s) 132, 325, 343, 583
Sau96I GGNCC 1 cut(s) 653
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 2 cut(s) 315, 593
SfcI CTRYAG 1 cut(s) 736
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
Sse9I AATT 7 cut(s) 126, 158, 285, 516, 548, 603, 790
SsiI CCGC 3 cut(s) 637, 656, 766
SspMI CTAG 2 cut(s) 182, 671
StyD4I CCNGG 2 cut(s) 313, 591
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 1 cut(s) 99
TaqI TCGA 3 cut(s) 162, 457, 770
TasI AATT 7 cut(s) 126, 158, 285, 516, 548, 603, 790
TatI WGTACW 2 cut(s) 433, 721
TfiI GAWTC 1 cut(s) 463
Tru1I TTAA 2 cut(s) 264, 393
Tru9I TTAA 2 cut(s) 264, 393
TscAI CASTG 2 cut(s) 335, 378
TseFI GTSAC 2 cut(s) 108, 201
TseI GCWGC 4 cut(s) 193, 473, 476, 763
Tsp45I GTSAC 2 cut(s) 108, 201
TspDTI ATGAA 2 cut(s) 17, 95
TspRI CASTG 2 cut(s) 335, 378
XapI RAATTY 2 cut(s) 158, 548
XbaI TCTAGA 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 93
XspI CTAG 2 cut(s) 182, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.