Rh7BG172000

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0014495
Physical Location & Seq
Forward (+)
13397525 .. 13400328
2804 bp
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UTR
Exon/CDS
Intron
Rh7BG172000.1

Sequence Viewer

Length: 555 bp
ATGAAGCTCAAAGTATACGTGGATAGGATGTCACAACCTTCGCGTGCAATTTTGATCTTCTGCAAGGTCAATGGTATAGAATTCGAGGAGGTGCAAATCAACCTAGCCAAACGCCAGCAAGTGTCACCTGATTTCAAAATGACATATGTTCTAAATACCGTACTTGCACCTGTGTTTGGACTTCCATTGAATCCACAAGCAGCTGCTGAAGCTGAGAAACTTCTGTCCTCCTCTTTGTCAAAAATTGAGTCTATTTGGCTCAAAGGGAATGGAAAGTTCTTGTTGGGTGGCTTTCAACCATCCATAGCAGATCTCAGCCTGGTTTGTGAAATTATGCAACTTGAGCTTTTGGACGAGAACGACCGCAGTCAGATACTTGGCCCACACAAGAAAGTTCTAGAGTGGATTGAGAATACAAAAAATGCGACAGGACCTCACTTTGAAGAAGTACATCAAGTCCTCTACAGAGCCAAAACGAGATTTCAAGAGCAGCGGTCGATGGGAGGAAACAACACTAATTCGAGCAAAAGATTGGGTTTGCCTTCAAAGATGTGA

Protein Analysis

184

Amino Acids

20.73

Weight (kDa)

9.08

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_C_2 PF13410 65 - 137 8e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41210
fragaria_vesca FvH4_5g02100
malus_domestica MD06G1081000.v1.1
prunus_persica Prupe.5G098100_v2.0.a1
pyrus_communis pycom06g07800
rosa_chinensis RchiOBHm_Chr7g0198031 RchiOBHm_Chr7g0198101
rosa_laevigata RLG00000003889 RLG00000003891
rosa_multiflora Rmu_sc0000221.1_g000031
rosa_roxburghii Rroxscaffold_3G00258000
rosa_rugosa Rorug07G0045600
rosa_samantha Rh7AG168900 Rh7BG172000 Rh7CG178500 Rh7DG171300
rosa_wichuraiana Rw7G014770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 15
AccII CGCG 1 cut(s) 43
AciI CCGC 2 cut(s) 364, 493
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 228
AfaI GTAC 2 cut(s) 162, 450
AfiI CCNNNNNNNGG 1 cut(s) 176
AgsI TTSAA 6 cut(s) 136, 190, 296, 443, 485, 546
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 4 cut(s) 7, 203, 212, 346
AluI AGCT 4 cut(s) 7, 203, 212, 346
AlwNI CAGNNNCTG 1 cut(s) 206
AoxI GGCC 1 cut(s) 379
ApeKI GCWGC 3 cut(s) 200, 203, 490
ApoI RAATTY 1 cut(s) 80
ArsI GACNNNNNNTTYG 2 cut(s) 233, 265
AspS9I GGNCC 2 cut(s) 380, 431
AsuHPI GGTGA 1 cut(s) 117
AvaII GGWCC 1 cut(s) 431
BbvI GCAGC 3 cut(s) 190, 212, 502
BccI CCATC 2 cut(s) 307, 493
BciT130I CCWGG 1 cut(s) 320
BfaI CTAG 2 cut(s) 104, 398
BfmI CTRYAG 1 cut(s) 463
BglII AGATCT 1 cut(s) 310
BisI GCNGC 3 cut(s) 201, 204, 491
BlsI GCNGC 3 cut(s) 202, 205, 492
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 380, 431
BmrFI CCNGG 1 cut(s) 320
BoxI GACNNNNGTC 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 362
BsaAI YACGTR 1 cut(s) 19
Bsc4I CCNNNNNNNGG 1 cut(s) 176
BseBI CCWGG 1 cut(s) 320
BseGI GGATG 2 cut(s) 33, 299
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMII CTCAG 2 cut(s) 204, 328
BseRI GAGGAG 2 cut(s) 101, 220
BseXI GCAGC 3 cut(s) 190, 212, 502
Bsh1236I CGCG 1 cut(s) 43
Bsh1285I CGRYCG 2 cut(s) 364, 497
BshFI GGCC 1 cut(s) 381
BsiEI CGRYCG 2 cut(s) 364, 497
BslI CCNNNNNNNGG 1 cut(s) 176
BsnI GGCC 1 cut(s) 381
Bsp143I GATC 2 cut(s) 54, 310
BspACI CCGC 2 cut(s) 364, 493
BspANI GGCC 1 cut(s) 381
BspCNI CTCAG 2 cut(s) 205, 327
BspFNI CGCG 1 cut(s) 43
BssMI GATC 2 cut(s) 54, 310
BssNAI GTATAC 1 cut(s) 16
Bst1107I GTATAC 1 cut(s) 16
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 1 cut(s) 160
BstBAI YACGTR 1 cut(s) 19
BstC8I GCNNGC 2 cut(s) 45, 116
BstDEI CTNAG 2 cut(s) 213, 314
BstF5I GGATG 2 cut(s) 33, 299
BstFNI CGCG 1 cut(s) 43
BstKTI GATC 2 cut(s) 57, 313
BstMBI GATC 2 cut(s) 54, 310
BstMCI CGRYCG 2 cut(s) 364, 497
BstMWI GCNNNNNNNGC 2 cut(s) 209, 343
BstNI CCWGG 1 cut(s) 320
BstPAI GACNNNNGTC 1 cut(s) 366
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 463
BstUI CGCG 1 cut(s) 43
BstV1I GCAGC 3 cut(s) 190, 212, 502
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BstZ17I GTATAC 1 cut(s) 16
BsuRI GGCC 1 cut(s) 381
BtsCI GGATG 2 cut(s) 33, 299
Cac8I GCNNGC 2 cut(s) 45, 116
CaiI CAGNNNCTG 1 cut(s) 206
Cfr13I GGNCC 2 cut(s) 380, 431
Csp6I GTAC 2 cut(s) 161, 449
CviQI GTAC 2 cut(s) 161, 449
DdeI CTNAG 2 cut(s) 213, 314
DpnI GATC 2 cut(s) 56, 312
DpnII GATC 2 cut(s) 54, 310
Eco47I GGWCC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 431
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 318
FaiI YATR 6 cut(s) 16, 77, 145, 147, 305, 335
FauNDI CATATG 1 cut(s) 145
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 3 cut(s) 201, 204, 491
FokI GGATG 2 cut(s) 40, 286
Fsp4HI GCNGC 3 cut(s) 201, 204, 491
FspBI CTAG 2 cut(s) 104, 398
GluI GCNGC 3 cut(s) 201, 204, 491
HaeIII GGCC 1 cut(s) 381
HinfI GANTC 2 cut(s) 190, 248
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 1 cut(s) 372
Hpy188III TCNNGA 2 cut(s) 398, 485
Hpy8I GTNNAC 1 cut(s) 16
HpyAV CCTTC 2 cut(s) 48, 552
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 5 cut(s) 47, 63, 94, 167, 337
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 343
HpyF3I CTNAG 2 cut(s) 213, 314
HpySE526I ACGT 1 cut(s) 18
Kzo9I GATC 2 cut(s) 54, 310
LpnPI CCDG 6 cut(s) 128, 141, 183, 305, 332, 414
Lsp1109I GCAGC 3 cut(s) 190, 212, 502
MaeI CTAG 2 cut(s) 104, 398
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 2 cut(s) 30, 123
MalI GATC 2 cut(s) 56, 312
MboI GATC 2 cut(s) 54, 310
MboII GAAGA 2 cut(s) 49, 455
MflI RGATCY 1 cut(s) 310
MluCI AATT 5 cut(s) 48, 80, 243, 330, 517
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 7 cut(s) 79, 82, 238, 241, 444, 470, 497
MspA1I CMGCKG 2 cut(s) 203, 493
MspR9I CCNGG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 1 cut(s) 43
MwoI GCNNNNNNNGC 2 cut(s) 209, 343
NdeI CATATG 1 cut(s) 145
NdeII GATC 2 cut(s) 54, 310
NmuCI GTSAC 2 cut(s) 30, 123
PfeI GAWTC 1 cut(s) 190
PkrI GCNGC 3 cut(s) 202, 205, 492
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
Ppu21I YACGTR 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 431
PshAI GACNNNNGTC 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 431
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspPI GGNCC 2 cut(s) 380, 431
PspPPI RGGWCCY 1 cut(s) 431
PstNI CAGNNNCTG 1 cut(s) 206
PsuI RGATCY 1 cut(s) 310
PvuII CAGCTG 1 cut(s) 203
RsaI GTAC 2 cut(s) 162, 450
RsaNI GTAC 2 cut(s) 161, 449
SatI GCNGC 3 cut(s) 201, 204, 491
Sau3AI GATC 2 cut(s) 54, 310
Sau96I GGNCC 2 cut(s) 380, 431
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 320
SfcI CTRYAG 1 cut(s) 463
SinI GGWCC 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 5 cut(s) 48, 80, 243, 330, 517
SsiI CCGC 2 cut(s) 364, 493
SspMI CTAG 2 cut(s) 104, 398
StyD4I CCNGG 1 cut(s) 318
TaaI ACNGT 1 cut(s) 160
TaiI ACGT 1 cut(s) 21
TaqI TCGA 3 cut(s) 84, 497, 521
TasI AATT 5 cut(s) 48, 80, 243, 330, 517
TatI WGTACW 1 cut(s) 448
TfiI GAWTC 1 cut(s) 190
TseFI GTSAC 2 cut(s) 30, 123
TseI GCWGC 3 cut(s) 200, 203, 490
Tsp45I GTSAC 2 cut(s) 30, 123
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 431
XapI RAATTY 1 cut(s) 80
XbaI TCTAGA 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 2 cut(s) 104, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.