RLG00000003889

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014495
Physical Location & Seq
Reverse (-)
54015847 .. 54020238
4392 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003889

Sequence Viewer

Length: 813 bp
ATGGTACCAACCCCAGTACCAACCATGACGGCCGTAGGAGTTGCTAGGCCTAAAGCACAAGGGCATGCAATGACCAAAATCCAGATCCATACTGAAGAGCAAGCTCAAAACTGTCAATTGAAGATGGTACCTAAGAATGCGGATAGCCGTGGAAGTATGATCACCTGCAGCATTAAGCCATTTGTTAACAAATGTATGGCGGGTGAAATTCTAGGACGTGACGACGTCGTTCGGCCAAAACTTGGCCCGACTCGACCCGAAAAGACCAAGGCCAACCCATCAAGCGCCGAACACAAAATGCCGCGGACGGTCAATGGTATAGAATTCGAGGAGCTGCAAATCAACATAGCCAAACGCCAGCAAATGTCACCTGATTTCAAAAAAATAAACCCTATGGGACAAGTTCCCGCTATTGCTGATGGGAGCTTTAATCTGTTTGAGAGTCATGCAATTGATGTCTATCTTGCTTGTGCGTTTCCTGGAGTAGCAGATCATTGGTATCCTGCTGATCTTTTCAGGAGAGCTAAAATCAACTCAGTGTTGGTTTGGCACCACTCTAACTTACGCCATGGTGCATCAACATATGTTCTAAATACTCTACTTGCACCTTTTCTTGGACTTCAATTGAATCCACAAGCAGCTGCTGAAGCTGAGAAACTTTTGTCCTCCTCTTTGTCAAAAATTGAGTCTATTTGGCTCAAAGGGAATGGAAAGTTCTTGTTGGTGGCTTTCAACCATCCATTGCAGATCTCAGCCTGGAGGGTTAGGATTGGGAAGGACATTGCTGCAAAGTACAATGAACACATCTACTGA

Protein Analysis

271

Amino Acids

29.91

Weight (kDa)

9.49

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 105 - 156 6.8e-10 Glutathione S-transferase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41210
fragaria_vesca FvH4_5g02100
malus_domestica MD06G1081000.v1.1
prunus_persica Prupe.5G098100_v2.0.a1
pyrus_communis pycom06g07800
rosa_chinensis RchiOBHm_Chr7g0198031 RchiOBHm_Chr7g0198101
rosa_laevigata RLG00000003889 RLG00000003891
rosa_multiflora Rmu_sc0000221.1_g000031
rosa_roxburghii Rroxscaffold_3G00258000
rosa_rugosa Rorug07G0045600
rosa_samantha Rh7AG168900 Rh7BG172000 Rh7CG178500 Rh7DG171300
rosa_wichuraiana Rw7G014770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 173
AatII GACGTC 1 cut(s) 228
Acc36I ACCTGC 1 cut(s) 173
Acc65I GGTACC 2 cut(s) 4, 127
AccB1I GGYRCC 3 cut(s) 4, 127, 549
AccB7I CCANNNNNTGG 1 cut(s) 242
AccII CGCG 1 cut(s) 304
AciI CCGC 5 cut(s) 140, 200, 302, 304, 408
AclWI GGATC 1 cut(s) 79
AcoI YGGCCR 2 cut(s) 30, 233
AcsI RAATTY 2 cut(s) 207, 323
AcuI CTGAAG 2 cut(s) 114, 666
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 4 cut(s) 6, 18, 129, 794
AfiI CCNNNNNNNGG 2 cut(s) 242, 614
AgsI TTSAA 5 cut(s) 121, 379, 623, 628, 733
AjiI CACGTC 1 cut(s) 218
AjnI CCWGG 2 cut(s) 478, 755
AluBI AGCT 6 cut(s) 104, 334, 426, 524, 641, 650
AluI AGCT 6 cut(s) 104, 334, 426, 524, 641, 650
AlwI GGATC 1 cut(s) 79
AlwNI CAGNNNCTG 1 cut(s) 644
AoxI GGCC 5 cut(s) 30, 47, 233, 244, 270
ApeKI GCWGC 5 cut(s) 168, 334, 638, 641, 785
ApoI RAATTY 2 cut(s) 207, 323
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
Asp718I GGTACC 2 cut(s) 4, 127
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 1 cut(s) 245
AsuHPI GGTGA 3 cut(s) 154, 215, 360
BaeI ACNNNNGTAYC 1 cut(s) 33
BanI GGYRCC 3 cut(s) 4, 127, 549
BbvI GCAGC 5 cut(s) 180, 321, 628, 650, 772
BccI CCATC 4 cut(s) 118, 286, 413, 744
BceAI ACGGC 3 cut(s) 17, 45, 132
BciT130I CCWGG 2 cut(s) 480, 757
BciVI GTATCC 1 cut(s) 510
BclI TGATCA 1 cut(s) 159
BfaI CTAG 2 cut(s) 45, 212
BfmI CTRYAG 1 cut(s) 166
BfoI RGCGCY 1 cut(s) 288
BfuAI ACCTGC 1 cut(s) 173
BfuI GTATCC 1 cut(s) 510
BglII AGATCT 1 cut(s) 747
BisI GCNGC 6 cut(s) 169, 302, 335, 639, 642, 786
BlsI GCNGC 6 cut(s) 170, 303, 336, 640, 643, 787
Bme1390I CCNGG 2 cut(s) 480, 757
BmgBI CACGTC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 3 cut(s) 6, 129, 551
BmrFI CCNGG 2 cut(s) 480, 757
BmrI ACTGGG 1 cut(s) 8
BmsI GCATC 1 cut(s) 584
BmuI ACTGGG 1 cut(s) 8
BpmI CTGGAG 2 cut(s) 501, 778
BsaBI GATNNNNATC 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 225
BsaJI CCNNGG 4 cut(s) 148, 267, 302, 568
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 614
Bse1I ACTGG 1 cut(s) 14
Bse3DI GCAATG 3 cut(s) 75, 740, 780
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 2 cut(s) 480, 757
BseDI CCNNGG 4 cut(s) 148, 267, 302, 568
BseGI GGATG 1 cut(s) 736
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 2 cut(s) 242, 614
BseMI GCAATG 3 cut(s) 75, 740, 780
BseMII CTCAG 3 cut(s) 549, 642, 765
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 2 cut(s) 344, 658
BseX3I CGGCCG 1 cut(s) 30
BseXI GCAGC 5 cut(s) 180, 321, 628, 650, 772
Bsh1236I CGCG 1 cut(s) 304
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 5 cut(s) 32, 49, 235, 246, 272
BshNI GGYRCC 3 cut(s) 4, 127, 549
BsiEI CGRYCG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 411
BslI CCNNNNNNNGG 2 cut(s) 242, 614
BsmFI GGGAC 1 cut(s) 411
BsmI GAATGC 1 cut(s) 142
BsnI GGCC 5 cut(s) 32, 49, 235, 246, 272
Bsp143I GATC 5 cut(s) 84, 159, 490, 508, 747
Bsp19I CCATGG 1 cut(s) 568
BspACI CCGC 5 cut(s) 140, 200, 302, 304, 408
BspANI GGCC 5 cut(s) 32, 49, 235, 246, 272
BspCNI CTCAG 3 cut(s) 548, 643, 764
BspFNI CGCG 1 cut(s) 304
BspLI GGNNCC 3 cut(s) 6, 129, 551
BspMAI CTGCAG 1 cut(s) 170
BspMI ACCTGC 1 cut(s) 173
BspPI GGATC 1 cut(s) 79
BspQI GCTCTTC 1 cut(s) 90
BspT107I GGYRCC 3 cut(s) 4, 127, 549
BsrDI GCAATG 3 cut(s) 75, 740, 780
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 4 cut(s) 148, 267, 302, 568
BssMI GATC 5 cut(s) 84, 159, 490, 508, 747
BssNI GRCGYC 1 cut(s) 225
BssT1I CCWWGG 2 cut(s) 267, 568
Bst2UI CCWGG 2 cut(s) 480, 757
Bst4CI ACNGT 2 cut(s) 113, 310
Bst6I CTCTTC 1 cut(s) 90
BstACI GRCGYC 1 cut(s) 225
BstC8I GCNNGC 3 cut(s) 66, 102, 359
BstDEI CTNAG 4 cut(s) 132, 535, 651, 751
BstDSI CCRYGG 3 cut(s) 148, 302, 568
BstF5I GGATG 1 cut(s) 736
BstFNI CGCG 1 cut(s) 304
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 5 cut(s) 87, 162, 493, 511, 750
BstMBI GATC 5 cut(s) 84, 159, 490, 508, 747
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 1 cut(s) 647
BstNI CCWGG 2 cut(s) 480, 757
BstNSI RCATGY 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 478, 755
BstSFI CTRYAG 1 cut(s) 166
BstUI CGCG 1 cut(s) 304
BstV1I GCAGC 5 cut(s) 180, 321, 628, 650, 772
BstX2I RGATCY 2 cut(s) 84, 747
BstYI RGATCY 2 cut(s) 84, 747
BstZI CGGCCG 1 cut(s) 30
BsuI GTATCC 1 cut(s) 510
BsuRI GGCC 5 cut(s) 32, 49, 235, 246, 272
BtgI CCRYGG 3 cut(s) 148, 302, 568
BtrI CACGTC 1 cut(s) 218
BtsCI GGATG 1 cut(s) 736
BtsIMutI CAGTG 1 cut(s) 543
BveI ACCTGC 1 cut(s) 173
Cac8I GCNNGC 3 cut(s) 66, 102, 359
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 245
Cfr42I CCGCGG 1 cut(s) 305
Csp6I GTAC 4 cut(s) 5, 17, 128, 793
CviAII CATG 4 cut(s) 25, 65, 446, 569
CviQI GTAC 4 cut(s) 5, 17, 128, 793
DdeI CTNAG 4 cut(s) 132, 535, 651, 751
DpnI GATC 5 cut(s) 86, 161, 492, 510, 749
DpnII GATC 5 cut(s) 84, 159, 490, 508, 747
EaeI YGGCCR 2 cut(s) 30, 233
EagI CGGCCG 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
EclXI CGGCCG 1 cut(s) 30
Eco130I CCWWGG 2 cut(s) 267, 568
Eco147I AGGCCT 1 cut(s) 49
Eco52I CGGCCG 1 cut(s) 30
Eco57I CTGAAG 2 cut(s) 114, 666
EcoRI GAATTC 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 478, 755
EcoT14I CCWWGG 2 cut(s) 267, 568
ErhI CCWWGG 2 cut(s) 267, 568
FaeI CATG 4 cut(s) 28, 68, 449, 572
FaqI GGGAC 1 cut(s) 411
FatI CATG 4 cut(s) 24, 64, 445, 568
FauI CCCGC 2 cut(s) 193, 415
FauNDI CATATG 1 cut(s) 583
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 6 cut(s) 169, 302, 335, 639, 642, 786
FokI GGATG 1 cut(s) 723
Fsp4HI GCNGC 6 cut(s) 169, 302, 335, 639, 642, 786
FspBI CTAG 2 cut(s) 45, 212
GlaI GCGC 1 cut(s) 286
GluI GCNGC 6 cut(s) 169, 302, 335, 639, 642, 786
GsuI CTGGAG 2 cut(s) 501, 778
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 5 cut(s) 32, 49, 235, 246, 272
HhaI GCGC 1 cut(s) 287
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 4 cut(s) 28, 68, 449, 572
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 4 cut(s) 250, 442, 628, 686
HpaI GTTAAC 1 cut(s) 187
HphI GGTGA 3 cut(s) 154, 215, 360
Hpy166II GTNNAC 1 cut(s) 187
Hpy188III TCNNGA 2 cut(s) 82, 517
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 2 cut(s) 227, 230
HpyAV CCTTC 1 cut(s) 769
HpyCH4III ACNGT 2 cut(s) 113, 310
HpyCH4IV ACGT 2 cut(s) 217, 225
HpyCH4V TGCA 8 cut(s) 68, 168, 337, 449, 575, 605, 745, 788
HpyF10VI GCNNNNNNNGC 1 cut(s) 647
HpyF3I CTNAG 4 cut(s) 132, 535, 651, 751
HpySE526I ACGT 2 cut(s) 217, 225
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 4 cut(s) 28, 68, 449, 572
HspAI GCGC 1 cut(s) 285
KpnI GGTACC 2 cut(s) 8, 131
Ksp22I TGATCA 1 cut(s) 159
KspAI GTTAAC 1 cut(s) 187
KspI CCGCGG 1 cut(s) 305
Kzo9I GATC 5 cut(s) 84, 159, 490, 508, 747
LguI GCTCTTC 1 cut(s) 90
LmnI GCTCC 2 cut(s) 331, 423
Lsp1109I GCAGC 5 cut(s) 180, 321, 628, 650, 772
LweI GCATC 1 cut(s) 584
MaeI CTAG 2 cut(s) 45, 212
MaeII ACGT 2 cut(s) 217, 225
MaeIII GTNAC 2 cut(s) 218, 366
MalI GATC 5 cut(s) 86, 161, 492, 510, 749
MboI GATC 5 cut(s) 84, 159, 490, 508, 747
MboII GAAGA 2 cut(s) 107, 133
MfeI CAATTG 3 cut(s) 116, 450, 623
MflI RGATCY 2 cut(s) 84, 747
MluCI AATT 6 cut(s) 116, 207, 323, 450, 623, 681
MlyI GAGTC 3 cut(s) 244, 451, 695
MnlI CCTC 4 cut(s) 322, 676, 679, 753
MseI TTAA 3 cut(s) 174, 186, 429
MspA1I CMGCKG 2 cut(s) 304, 641
MspR9I CCNGG 2 cut(s) 480, 757
MunI CAATTG 3 cut(s) 116, 450, 623
Mva1269I GAATGC 1 cut(s) 142
MvaI CCWGG 2 cut(s) 480, 757
MvnI CGCG 1 cut(s) 304
MwoI GCNNNNNNNGC 1 cut(s) 647
NcoI CCATGG 1 cut(s) 568
NdeI CATATG 1 cut(s) 583
NdeII GATC 5 cut(s) 84, 159, 490, 508, 747
NlaIII CATG 4 cut(s) 28, 68, 449, 572
NlaIV GGNNCC 3 cut(s) 6, 129, 551
NmuCI GTSAC 2 cut(s) 218, 366
NspI RCATGY 1 cut(s) 68
PaeI GCATGC 1 cut(s) 68
PaqCI CACCTGC 1 cut(s) 173
PceI AGGCCT 1 cut(s) 49
PciSI GCTCTTC 1 cut(s) 90
PctI GAATGC 1 cut(s) 142
PfeI GAWTC 1 cut(s) 628
PflFI GACNNNGTC 1 cut(s) 224
PflMI CCANNNNNTGG 1 cut(s) 242
PfoI TCCNGGA 1 cut(s) 478
PkrI GCNGC 6 cut(s) 170, 303, 336, 640, 643, 787
PleI GAGTC 3 cut(s) 244, 450, 694
PpsI GAGTC 3 cut(s) 244, 450, 694
Psp6I CCWGG 2 cut(s) 478, 755
PspGI CCWGG 2 cut(s) 478, 755
PspN4I GGNNCC 3 cut(s) 6, 129, 551
PspPI GGNCC 1 cut(s) 245
PstI CTGCAG 1 cut(s) 170
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 2 cut(s) 84, 747
PsyI GACNNNGTC 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 641
RsaI GTAC 4 cut(s) 6, 18, 129, 794
RsaNI GTAC 4 cut(s) 5, 17, 128, 793
SacII CCGCGG 1 cut(s) 305
SapI GCTCTTC 1 cut(s) 90
SaqAI TTAA 3 cut(s) 174, 186, 429
SatI GCNGC 6 cut(s) 169, 302, 335, 639, 642, 786
Sau3AI GATC 5 cut(s) 84, 159, 490, 508, 747
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 3 cut(s) 244, 451, 695
ScrFI CCNGG 2 cut(s) 480, 757
SfaNI GCATC 1 cut(s) 584
SfcI CTRYAG 1 cut(s) 166
Sfr303I CCGCGG 1 cut(s) 305
SgrBI CCGCGG 1 cut(s) 305
SphI GCATGC 1 cut(s) 68
Sse9I AATT 6 cut(s) 116, 207, 323, 450, 623, 681
SseBI AGGCCT 1 cut(s) 49
SsiI CCGC 5 cut(s) 140, 200, 302, 304, 408
SspMI CTAG 2 cut(s) 45, 212
StuI AGGCCT 1 cut(s) 49
StyD4I CCNGG 2 cut(s) 478, 755
StyI CCWWGG 2 cut(s) 267, 568
TaaI ACNGT 2 cut(s) 113, 310
TaiI ACGT 2 cut(s) 220, 228
TaqI TCGA 2 cut(s) 253, 327
TasI AATT 6 cut(s) 116, 207, 323, 450, 623, 681
TatI WGTACW 1 cut(s) 792
TauI GCSGC 1 cut(s) 304
TfiI GAWTC 1 cut(s) 628
Tru1I TTAA 3 cut(s) 174, 186, 429
Tru9I TTAA 3 cut(s) 174, 186, 429
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 2 cut(s) 218, 366
TseI GCWGC 5 cut(s) 168, 334, 638, 641, 785
Tsp45I GTSAC 2 cut(s) 218, 366
TspDTI ATGAA 1 cut(s) 813
TspRI CASTG 1 cut(s) 543
Tth111I GACNNNGTC 1 cut(s) 224
Van91I CCANNNNNTGG 1 cut(s) 242
XapI RAATTY 2 cut(s) 207, 323
XceI RCATGY 1 cut(s) 68
XspI CTAG 2 cut(s) 45, 212
ZraI GACGTC 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.