RLG00000003891

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014495
Physical Location & Seq
Reverse (-)
54031951 .. 54034382
2432 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003891

Sequence Viewer

Length: 756 bp
ATGAAGCTCAAAGTATACGTGGATAGGATGTCACAACCTTCGCGTGCAATTTTGATCTTCTGCAAGGTCAATGGTATAGAATTCGAGGAGGTGCAAATCAACATAGCCAAACGCCAGCAAATGTCACCTGATTTCAAAAAAATAAACCCAATGGGACAAGTTCCCGCTATTGCTGATGGGAGGTTTAATCTGTTTGAAAGCCATGCAATTCTTGTCTATCTTGCTTGTGCGTTTCCTGGAGTAGCAGATCACTGGTATCCTGCTGATCTTTTCAGGAGAGCTAAAATCAACTCAGTGTTGGATTGGCACCACTCTAACTTACGCCGTGGTGCAATGACATATGTTCTAAATACCGTACTTGCACCTGTGTTTGGACTTCGATTGAATCCGCAAGCTGCTGCTGAAGCTGAGAAACTTCTGTCCTCCTCTTTGTCAAAAATTGAGTCTATTTGGCTCAAAGGGAATGGAAAGTTCTTGTTGGGTGGCTTTCAACCATCCATAGCAGATCTCAGCCTGGTTTGTGAAATTATGCAACTTGAGCTTTTGGACGAGAACGACCGCAGCCGGATACTTGGCCCGCACAAGAAAGTTCAAGAGTGGATTGAGAATACAAAAAATGCGACGAGGCCTCACTTTGAAGAAGTACATCAAGTCCTCTACAGAGCCAAAATGAGATTTCAAGAGCAGCGGTCGCAGTCGATGGGAGGAAACAACACTAATTCGAGCAAAAGATTGGGTTTGCCTTCAAAGATGTGA

Protein Analysis

252

Amino Acids

28.54

Weight (kDa)

9.54

Isoelectric Point (pI)

54.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 1 - 75 3.1e-14 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 7 - 80 4.2e-08 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 14 - 75 2.7e-09 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 130 - 202 6.1e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41210
fragaria_vesca FvH4_5g02100
malus_domestica MD06G1081000.v1.1
prunus_persica Prupe.5G098100_v2.0.a1
pyrus_communis pycom06g07800
rosa_chinensis RchiOBHm_Chr7g0198031 RchiOBHm_Chr7g0198101
rosa_laevigata RLG00000003889 RLG00000003891
rosa_multiflora Rmu_sc0000221.1_g000031
rosa_roxburghii Rroxscaffold_3G00258000
rosa_rugosa Rorug07G0045600
rosa_samantha Rh7AG168900 Rh7BG172000 Rh7CG178500 Rh7DG171300
rosa_wichuraiana Rw7G014770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 306
AccI GTMKAC 1 cut(s) 15
AccII CGCG 1 cut(s) 43
AciI CCGC 5 cut(s) 165, 389, 559, 578, 688
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 423
AfaI GTAC 2 cut(s) 357, 645
AfiI CCNNNNNNNGG 1 cut(s) 371
AgsI TTSAA 8 cut(s) 136, 197, 385, 491, 593, 638, 680, 747
AjnI CCWGG 2 cut(s) 235, 513
AluBI AGCT 5 cut(s) 7, 281, 395, 407, 541
AluI AGCT 5 cut(s) 7, 281, 395, 407, 541
AoxI GGCC 2 cut(s) 574, 626
ApeKI GCWGC 4 cut(s) 395, 398, 561, 685
ApoI RAATTY 1 cut(s) 80
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 1 cut(s) 117
BanI GGYRCC 1 cut(s) 306
BbvI GCAGC 4 cut(s) 382, 385, 573, 697
BccI CCATC 3 cut(s) 170, 502, 694
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 2 cut(s) 237, 515
BciVI GTATCC 2 cut(s) 267, 561
BfmI CTRYAG 1 cut(s) 658
BfuI GTATCC 2 cut(s) 267, 561
BglII AGATCT 1 cut(s) 505
BisI GCNGC 4 cut(s) 396, 399, 562, 686
BlsI GCNGC 4 cut(s) 397, 400, 563, 687
Bme1390I CCNGG 2 cut(s) 237, 515
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 2 cut(s) 237, 515
BpmI CTGGAG 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 557
BsaAI YACGTR 1 cut(s) 19
BsaJI CCNNGG 1 cut(s) 325
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 2 cut(s) 237, 515
BseDI CCNNGG 1 cut(s) 325
BseGI GGATG 2 cut(s) 33, 494
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 3 cut(s) 306, 399, 523
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 2 cut(s) 101, 415
BseXI GCAGC 4 cut(s) 382, 385, 573, 697
Bsh1236I CGCG 1 cut(s) 43
Bsh1285I CGRYCG 2 cut(s) 559, 692
BshFI GGCC 2 cut(s) 576, 628
BshNI GGYRCC 1 cut(s) 306
BsiEI CGRYCG 2 cut(s) 559, 692
BsiSI CCGG 1 cut(s) 565
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 2 cut(s) 576, 628
Bsp143I GATC 4 cut(s) 54, 247, 265, 505
BspACI CCGC 5 cut(s) 165, 389, 559, 578, 688
BspANI GGCC 2 cut(s) 576, 628
BspCNI CTCAG 3 cut(s) 305, 400, 522
BspFNI CGCG 1 cut(s) 43
BspLI GGNNCC 1 cut(s) 308
BspT107I GGYRCC 1 cut(s) 306
BsrDI GCAATG 1 cut(s) 339
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 325
BssMI GATC 4 cut(s) 54, 247, 265, 505
BssNAI GTATAC 1 cut(s) 16
Bst1107I GTATAC 1 cut(s) 16
Bst2UI CCWGG 2 cut(s) 237, 515
Bst4CI ACNGT 1 cut(s) 355
BstBAI YACGTR 1 cut(s) 19
BstC8I GCNNGC 4 cut(s) 45, 116, 393, 578
BstDEI CTNAG 3 cut(s) 292, 408, 509
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 2 cut(s) 33, 494
BstFNI CGCG 1 cut(s) 43
BstKTI GATC 4 cut(s) 57, 250, 268, 508
BstMBI GATC 4 cut(s) 54, 247, 265, 505
BstMCI CGRYCG 2 cut(s) 559, 692
BstMWI GCNNNNNNNGC 3 cut(s) 404, 538, 691
BstNI CCWGG 2 cut(s) 237, 515
BstSCI CCNGG 2 cut(s) 235, 513
BstSFI CTRYAG 1 cut(s) 658
BstUI CGCG 1 cut(s) 43
BstV1I GCAGC 4 cut(s) 382, 385, 573, 697
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
BstZ17I GTATAC 1 cut(s) 16
BsuI GTATCC 2 cut(s) 267, 561
BsuRI GGCC 2 cut(s) 576, 628
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 2 cut(s) 33, 494
BtsIMutI CAGTG 2 cut(s) 250, 300
Cac8I GCNNGC 4 cut(s) 45, 116, 393, 578
Cfr13I GGNCC 1 cut(s) 575
Csp6I GTAC 2 cut(s) 356, 644
CviAII CATG 1 cut(s) 203
CviQI GTAC 2 cut(s) 356, 644
DdeI CTNAG 3 cut(s) 292, 408, 509
DpnI GATC 4 cut(s) 56, 249, 267, 507
DpnII GATC 4 cut(s) 54, 247, 265, 505
Eco147I AGGCCT 1 cut(s) 628
Eco57I CTGAAG 1 cut(s) 423
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 2 cut(s) 235, 513
FaeI CATG 1 cut(s) 206
FaiI YATR 8 cut(s) 16, 77, 104, 204, 340, 342, 500, 530
FaqI GGGAC 1 cut(s) 168
FatI CATG 1 cut(s) 202
FauI CCCGC 2 cut(s) 172, 585
FauNDI CATATG 1 cut(s) 340
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 4 cut(s) 396, 399, 562, 686
FokI GGATG 2 cut(s) 40, 481
Fsp4HI GCNGC 4 cut(s) 396, 399, 562, 686
GluI GCNGC 4 cut(s) 396, 399, 562, 686
GsuI CTGGAG 1 cut(s) 258
HaeIII GGCC 2 cut(s) 576, 628
HapII CCGG 1 cut(s) 565
Hin1II CATG 1 cut(s) 206
HinfI GANTC 2 cut(s) 385, 443
HpaII CCGG 1 cut(s) 565
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 16
Hpy188III TCNNGA 3 cut(s) 274, 593, 680
Hpy8I GTNNAC 1 cut(s) 16
Hpy99I CGWCG 1 cut(s) 625
HpyAV CCTTC 2 cut(s) 48, 753
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 7 cut(s) 47, 63, 94, 206, 332, 362, 532
HpyF10VI GCNNNNNNNGC 3 cut(s) 404, 538, 691
HpyF3I CTNAG 3 cut(s) 292, 408, 509
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 1 cut(s) 206
Kzo9I GATC 4 cut(s) 54, 247, 265, 505
Lsp1109I GCAGC 4 cut(s) 382, 385, 573, 697
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 2 cut(s) 30, 123
MalI GATC 4 cut(s) 56, 249, 267, 507
MboI GATC 4 cut(s) 54, 247, 265, 505
MboII GAAGA 2 cut(s) 49, 650
MflI RGATCY 1 cut(s) 505
MluCI AATT 6 cut(s) 48, 80, 207, 438, 525, 718
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 9 cut(s) 79, 82, 174, 433, 436, 618, 639, 665, 698
MseI TTAA 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 688
MspI CCGG 1 cut(s) 565
MspR9I CCNGG 2 cut(s) 237, 515
MvaI CCWGG 2 cut(s) 237, 515
MvnI CGCG 1 cut(s) 43
MwoI GCNNNNNNNGC 3 cut(s) 404, 538, 691
NdeI CATATG 1 cut(s) 340
NdeII GATC 4 cut(s) 54, 247, 265, 505
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 2 cut(s) 30, 123
PceI AGGCCT 1 cut(s) 628
PfeI GAWTC 1 cut(s) 385
PfoI TCCNGGA 1 cut(s) 235
PkrI GCNGC 4 cut(s) 397, 400, 563, 687
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
Ppu21I YACGTR 1 cut(s) 19
Psp6I CCWGG 2 cut(s) 235, 513
PspGI CCWGG 2 cut(s) 235, 513
PspN4I GGNNCC 1 cut(s) 308
PspPI GGNCC 1 cut(s) 575
PsuI RGATCY 1 cut(s) 505
RsaI GTAC 2 cut(s) 357, 645
RsaNI GTAC 2 cut(s) 356, 644
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 4 cut(s) 396, 399, 562, 686
Sau3AI GATC 4 cut(s) 54, 247, 265, 505
Sau96I GGNCC 1 cut(s) 575
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 237, 515
SfcI CTRYAG 1 cut(s) 658
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 6 cut(s) 48, 80, 207, 438, 525, 718
SseBI AGGCCT 1 cut(s) 628
SsiI CCGC 5 cut(s) 165, 389, 559, 578, 688
StuI AGGCCT 1 cut(s) 628
StyD4I CCNGG 2 cut(s) 235, 513
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 1 cut(s) 21
TaqI TCGA 4 cut(s) 84, 379, 698, 722
TasI AATT 6 cut(s) 48, 80, 207, 438, 525, 718
TatI WGTACW 1 cut(s) 643
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TscAI CASTG 2 cut(s) 257, 300
TseFI GTSAC 2 cut(s) 30, 123
TseI GCWGC 4 cut(s) 395, 398, 561, 685
Tsp45I GTSAC 2 cut(s) 30, 123
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 257, 300
XapI RAATTY 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.