Rw7G014770

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014495
Physical Location & Seq
Forward (+)
14459985 .. 14462439
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G014770.1

Sequence Viewer

Length: 750 bp
ATGAAGCTCAAAGTATACGTGGATAGGATGTCACAACCTTCGCGTGCAATTTTGATCTTCTGCAAGGTCAATGGAATAGAATTCGAGGAGGTGCAAATCAACCTAGCCAAACAGCAGCAAAAGTCACCCGATTTCAAAAAAATAAACCCTATGGGACAAGTTCCCGCTATTGCTGATGGGAGGTTTAATCTGTTTGAAAGCCATGCAATTCTTGTCTATCTTGCTTGTGCGTTTCCTGGAGTAGCAGATCACTGGTATCCTGCTGATCTTTTCAGGAGAGCTAAAATCAACTCGGTGCTGGATTGGCACCATTTTAACTTACGCCGTGGTGCAATGACATATGTTCTAAATACCGTACTTGCACCTGTGTTTGGACTTCCATTGAATCCACAAGCAGCTGCCGAAGCTGAGAAACTTCTGTCCTCCTCTTTGTCAAAAATTGAGTCTATTTGGCTCAAAGGGAATGGAAAGTTCTTGTTGGGTGGCTTTCAACCATCCATAGCAGATCTCAGCCTGGTTTGTGAAATTATGCAACTTGAGCTTTTGGACGAGAACGACCGCAGTCGGATACTTGGCCCGCACAAGAAAGTTCTAGAGTGGATTGAGAATACAAAAAATGCGACAAGACCTCACTTTGAAGAAGTACATCAAGTCCTCTACAGAGCCAAAACGAGATTTCAAGAGCAGCGGTCGATGGGAGCAAACAGCACTAATTCGAGCAAAAGATTGGGTTTGCCTTCAAAGATGTGA

Protein Analysis

249

Amino Acids

28.23

Weight (kDa)

9.42

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 1 - 75 1.7e-15 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 7 - 80 8.5e-09 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 14 - 75 4e-10 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 130 - 202 8.5e-06 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41210
fragaria_vesca FvH4_5g02100
malus_domestica MD06G1081000.v1.1
prunus_persica Prupe.5G098100_v2.0.a1
pyrus_communis pycom06g07800
rosa_chinensis RchiOBHm_Chr7g0198031 RchiOBHm_Chr7g0198101
rosa_laevigata RLG00000003889 RLG00000003891
rosa_multiflora Rmu_sc0000221.1_g000031
rosa_roxburghii Rroxscaffold_3G00258000
rosa_rugosa Rorug07G0045600
rosa_samantha Rh7AG168900 Rh7BG172000 Rh7CG178500 Rh7DG171300
rosa_wichuraiana Rw7G014770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 306
AccI GTMKAC 1 cut(s) 15
AccII CGCG 1 cut(s) 43
AciI CCGC 4 cut(s) 165, 559, 578, 688
AcsI RAATTY 1 cut(s) 80
AfaI GTAC 2 cut(s) 357, 645
AfiI CCNNNNNNNGG 1 cut(s) 371
AgsI TTSAA 7 cut(s) 136, 197, 385, 491, 638, 680, 741
AjnI CCWGG 2 cut(s) 235, 513
AluBI AGCT 5 cut(s) 7, 281, 398, 407, 541
AluI AGCT 5 cut(s) 7, 281, 398, 407, 541
AoxI GGCC 1 cut(s) 574
ApeKI GCWGC 4 cut(s) 115, 395, 398, 685
ApoI RAATTY 1 cut(s) 80
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 1 cut(s) 117
BanI GGYRCC 1 cut(s) 306
BbvI GCAGC 4 cut(s) 127, 385, 407, 697
BccI CCATC 3 cut(s) 170, 502, 688
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 2 cut(s) 237, 515
BciVI GTATCC 2 cut(s) 267, 561
BfaI CTAG 2 cut(s) 104, 593
BfmI CTRYAG 1 cut(s) 658
BfuI GTATCC 2 cut(s) 267, 561
BglII AGATCT 1 cut(s) 505
BisI GCNGC 4 cut(s) 116, 396, 399, 686
BlsI GCNGC 4 cut(s) 117, 397, 400, 687
Bme1390I CCNGG 2 cut(s) 237, 515
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 2 cut(s) 237, 515
BoxI GACNNNNGTC 1 cut(s) 561
BpmI CTGGAG 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 557
BsaAI YACGTR 1 cut(s) 19
BsaJI CCNNGG 1 cut(s) 325
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 2 cut(s) 237, 515
BseDI CCNNGG 1 cut(s) 325
BseGI GGATG 2 cut(s) 33, 494
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 2 cut(s) 399, 523
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 2 cut(s) 101, 415
BseXI GCAGC 4 cut(s) 127, 385, 407, 697
Bsh1236I CGCG 1 cut(s) 43
Bsh1285I CGRYCG 2 cut(s) 559, 692
BshFI GGCC 1 cut(s) 576
BshNI GGYRCC 1 cut(s) 306
BsiEI CGRYCG 2 cut(s) 559, 692
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 1 cut(s) 576
Bsp143I GATC 4 cut(s) 54, 247, 265, 505
BspACI CCGC 4 cut(s) 165, 559, 578, 688
BspANI GGCC 1 cut(s) 576
BspCNI CTCAG 2 cut(s) 400, 522
BspFNI CGCG 1 cut(s) 43
BspLI GGNNCC 1 cut(s) 308
BspT107I GGYRCC 1 cut(s) 306
BsrDI GCAATG 1 cut(s) 339
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 325
BssMI GATC 4 cut(s) 54, 247, 265, 505
BssNAI GTATAC 1 cut(s) 16
Bst1107I GTATAC 1 cut(s) 16
Bst2UI CCWGG 2 cut(s) 237, 515
Bst4CI ACNGT 1 cut(s) 355
BstBAI YACGTR 1 cut(s) 19
BstC8I GCNNGC 2 cut(s) 45, 578
BstDEI CTNAG 2 cut(s) 408, 509
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 2 cut(s) 33, 494
BstFNI CGCG 1 cut(s) 43
BstKTI GATC 4 cut(s) 57, 250, 268, 508
BstMBI GATC 4 cut(s) 54, 247, 265, 505
BstMCI CGRYCG 2 cut(s) 559, 692
BstMWI GCNNNNNNNGC 3 cut(s) 304, 404, 538
BstNI CCWGG 2 cut(s) 237, 515
BstPAI GACNNNNGTC 1 cut(s) 561
BstSCI CCNGG 2 cut(s) 235, 513
BstSFI CTRYAG 1 cut(s) 658
BstUI CGCG 1 cut(s) 43
BstV1I GCAGC 4 cut(s) 127, 385, 407, 697
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
BstZ17I GTATAC 1 cut(s) 16
BsuI GTATCC 2 cut(s) 267, 561
BsuRI GGCC 1 cut(s) 576
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 2 cut(s) 33, 494
BtsIMutI CAGTG 1 cut(s) 250
Cac8I GCNNGC 2 cut(s) 45, 578
Cfr13I GGNCC 1 cut(s) 575
Csp6I GTAC 2 cut(s) 356, 644
CviAII CATG 1 cut(s) 203
CviQI GTAC 2 cut(s) 356, 644
DdeI CTNAG 2 cut(s) 408, 509
DpnI GATC 4 cut(s) 56, 249, 267, 507
DpnII GATC 4 cut(s) 54, 247, 265, 505
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 2 cut(s) 235, 513
FaeI CATG 1 cut(s) 206
FaiI YATR 7 cut(s) 16, 152, 204, 340, 342, 500, 530
FaqI GGGAC 1 cut(s) 168
FatI CATG 1 cut(s) 202
FauI CCCGC 2 cut(s) 172, 585
FauNDI CATATG 1 cut(s) 340
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 4 cut(s) 116, 396, 399, 686
FokI GGATG 2 cut(s) 40, 481
Fsp4HI GCNGC 4 cut(s) 116, 396, 399, 686
FspBI CTAG 2 cut(s) 104, 593
GluI GCNGC 4 cut(s) 116, 396, 399, 686
GsuI CTGGAG 1 cut(s) 258
HaeIII GGCC 1 cut(s) 576
Hin1II CATG 1 cut(s) 206
HinfI GANTC 2 cut(s) 385, 443
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 1 cut(s) 567
Hpy188III TCNNGA 3 cut(s) 274, 593, 680
Hpy8I GTNNAC 1 cut(s) 16
HpyAV CCTTC 2 cut(s) 48, 747
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 7 cut(s) 47, 63, 94, 206, 332, 362, 532
HpyF10VI GCNNNNNNNGC 3 cut(s) 304, 404, 538
HpyF3I CTNAG 2 cut(s) 408, 509
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 1 cut(s) 206
Kzo9I GATC 4 cut(s) 54, 247, 265, 505
LmnI GCTCC 1 cut(s) 698
LpnPI CCDG 9 cut(s) 222, 238, 249, 259, 273, 284, 378, 500, 527
Lsp1109I GCAGC 4 cut(s) 127, 385, 407, 697
MaeI CTAG 2 cut(s) 104, 593
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 2 cut(s) 30, 123
MalI GATC 4 cut(s) 56, 249, 267, 507
MboI GATC 4 cut(s) 54, 247, 265, 505
MboII GAAGA 2 cut(s) 49, 650
MflI RGATCY 1 cut(s) 505
MluCI AATT 6 cut(s) 48, 80, 207, 438, 525, 712
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 545
MnlI CCTC 7 cut(s) 79, 82, 174, 433, 436, 639, 665
MseI TTAA 2 cut(s) 186, 315
MspA1I CMGCKG 2 cut(s) 398, 688
MspR9I CCNGG 2 cut(s) 237, 515
MvaI CCWGG 2 cut(s) 237, 515
MvnI CGCG 1 cut(s) 43
MwoI GCNNNNNNNGC 3 cut(s) 304, 404, 538
NdeI CATATG 1 cut(s) 340
NdeII GATC 4 cut(s) 54, 247, 265, 505
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 1 cut(s) 308
NmuCI GTSAC 2 cut(s) 30, 123
PfeI GAWTC 1 cut(s) 385
PfoI TCCNGGA 1 cut(s) 235
PkrI GCNGC 4 cut(s) 117, 397, 400, 687
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
Ppu21I YACGTR 1 cut(s) 19
PshAI GACNNNNGTC 1 cut(s) 561
Psp6I CCWGG 2 cut(s) 235, 513
PspGI CCWGG 2 cut(s) 235, 513
PspN4I GGNNCC 1 cut(s) 308
PspPI GGNCC 1 cut(s) 575
PsuI RGATCY 1 cut(s) 505
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 2 cut(s) 357, 645
RsaNI GTAC 2 cut(s) 356, 644
SaqAI TTAA 2 cut(s) 186, 315
SatI GCNGC 4 cut(s) 116, 396, 399, 686
Sau3AI GATC 4 cut(s) 54, 247, 265, 505
Sau96I GGNCC 1 cut(s) 575
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 237, 515
SfcI CTRYAG 1 cut(s) 658
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 6 cut(s) 48, 80, 207, 438, 525, 712
SsiI CCGC 4 cut(s) 165, 559, 578, 688
SspMI CTAG 2 cut(s) 104, 593
StyD4I CCNGG 2 cut(s) 235, 513
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 1 cut(s) 21
TaqI TCGA 3 cut(s) 84, 692, 716
TasI AATT 6 cut(s) 48, 80, 207, 438, 525, 712
TatI WGTACW 1 cut(s) 643
TfiI GAWTC 1 cut(s) 385
Tru1I TTAA 2 cut(s) 186, 315
Tru9I TTAA 2 cut(s) 186, 315
TscAI CASTG 1 cut(s) 257
TseFI GTSAC 2 cut(s) 30, 123
TseI GCWGC 4 cut(s) 115, 395, 398, 685
Tsp45I GTSAC 2 cut(s) 30, 123
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 257
XapI RAATTY 1 cut(s) 80
XbaI TCTAGA 1 cut(s) 592
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 2 cut(s) 104, 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.