RchiOBHm_Chr7g0233781

Belongs to the peptidase M18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0017800
Physical Location & Seq
Reverse (-)
58310749 .. 58312148
1400 bp
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UTR
Exon/CDS
Intron
PRQ20955

Sequence Viewer

Length: 495 bp
ATGGCCTACAATAAACTTACATCCAAGTGCCGTCGGCAAATTCCCACTCCACCACACTCTCTCTCTTTTTTCCTCCATTTTCGAAGAGATCGTGATCGCGCTTTCCACCTTATCGACGAGGCGAAGAAGCGTCTGCAAAGAGCGGGGTACGAGCAGGTTTTGGAGAGAGAGGATTGGAAATTGGAGGCTGGTAAAAAGTACTACTTCACCAGGAACTACTCCACCATTGTGGCTTTTGCAATTGGTAAAAAATTTGTTGCCGGAAATGGATTCCATATAGTTGGTGCTCATACTGATAGTCCTTGTCTCAAATTGAAGCCTGTTTCCGAGGTAGCTAAGGGTAGGTACTTGGAAGTGGGAGTCCAAACATATGGAGATGGCTTGTGGCATACCTGGTTCGACCGTGACTTGACAATTGCAGGAAGGGTGATAGTAAGAGAAGAGAAAGATGGTGTTGTTTCTTACTCTGGCAATTCACCTGGACAGGGATGTTAG

Protein Analysis

164

Amino Acids

18.77

Weight (kDa)

9.56

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M18 PF02127 34 - 150 6e-44 Aminopeptidase I zinc metalloprotease (M18)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017800)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0425041 RchiOBHm_Chr7g0233781
rosa_laevigata RLG00000000804 RLG00000015399
rosa_roxburghii Rroxscaffold_2G00092620
rosa_samantha Rh4CG034400 Rh4DG259400 Rh5AG471200 Rh6AG020500 Rh6BG016300 Rh7CG458700
rosa_wichuraiana Rw4G022290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 145
AccBSI CCGCTC 1 cut(s) 143
AccII CGCG 1 cut(s) 99
AciI CCGC 1 cut(s) 143
AcsI RAATTY 2 cut(s) 39, 251
AfaI GTAC 3 cut(s) 149, 200, 347
AfiI CCNNNNNNNGG 1 cut(s) 485
AgsI TTSAA 1 cut(s) 316
AjnI CCWGG 3 cut(s) 209, 392, 478
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 1 cut(s) 335
AluI AGCT 1 cut(s) 335
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 311
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 2 cut(s) 39, 251
AspLEI GCGC 1 cut(s) 101
AsuHPI GGTGA 3 cut(s) 199, 439, 468
AsuII TTCGAA 1 cut(s) 82
Bbv12I GWGCWC 1 cut(s) 289
BccI CCATC 2 cut(s) 371, 443
BceAI ACGGC 1 cut(s) 15
BciT130I CCWGG 3 cut(s) 211, 394, 480
BcoDI GTCTC 1 cut(s) 311
BfuAI ACCTGC 1 cut(s) 145
BmcAI AGTACT 1 cut(s) 200
Bme1390I CCNGG 3 cut(s) 211, 394, 480
BmrFI CCNGG 3 cut(s) 211, 394, 480
Bpu10I CCTNAGC 1 cut(s) 336
Bpu14I TTCGAA 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 327
Bsc4I CCNNNNNNNGG 1 cut(s) 485
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 3 cut(s) 211, 394, 480
BseDI CCNNGG 1 cut(s) 327
BseGI GGATG 2 cut(s) 20, 494
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 1 cut(s) 485
Bsh1236I CGCG 1 cut(s) 99
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 485
BsmAI GTCTC 1 cut(s) 311
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 82
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 2 cut(s) 88, 94
BspACI CCGC 1 cut(s) 143
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 99
BspMI ACCTGC 1 cut(s) 145
BspT104I TTCGAA 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 327
BssMI GATC 2 cut(s) 88, 94
Bst2UI CCWGG 3 cut(s) 211, 394, 480
Bst4CI ACNGT 1 cut(s) 404
Bst6I CTCTTC 2 cut(s) 79, 435
BstBI TTCGAA 1 cut(s) 82
BstDEI CTNAG 1 cut(s) 336
BstF5I GGATG 2 cut(s) 20, 494
BstFNI CGCG 1 cut(s) 99
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 2 cut(s) 91, 97
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 2 cut(s) 88, 94
BstMCI CGRYCG 1 cut(s) 403
BstNI CCWGG 3 cut(s) 211, 394, 480
BstSCI CCNGG 3 cut(s) 209, 392, 478
BstUI CGCG 1 cut(s) 99
BstXI CCANNNNNNTGG 3 cut(s) 229, 281, 371
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 20, 494
BveI ACCTGC 1 cut(s) 145
CfoI GCGC 1 cut(s) 101
CseI GACGC 1 cut(s) 119
CsiI ACCWGGT 1 cut(s) 392
Csp6I GTAC 3 cut(s) 148, 199, 346
CviJI RGCY 6 cut(s) 5, 188, 233, 319, 335, 381
CviKI_1 RGCY 6 cut(s) 5, 188, 233, 319, 335, 381
CviQI GTAC 3 cut(s) 148, 199, 346
DdeI CTNAG 1 cut(s) 336
DpnI GATC 2 cut(s) 90, 96
DpnII GATC 2 cut(s) 88, 94
Eam1104I CTCTTC 2 cut(s) 79, 435
EarI CTCTTC 2 cut(s) 79, 435
EcoRII CCWGG 3 cut(s) 209, 392, 478
FaiI YATR 6 cut(s) 276, 278, 291, 370, 372, 390
FalI AAGNNNNNCTT 2 cut(s) 188, 220
FauI CCCGC 1 cut(s) 136
FauNDI CATATG 1 cut(s) 370
FokI GGATG 1 cut(s) 7
GlaI GCGC 1 cut(s) 100
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 261
HgaI GACGC 1 cut(s) 119
HhaI GCGC 1 cut(s) 101
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HinfI GANTC 2 cut(s) 270, 360
HpaII CCGG 1 cut(s) 261
HphI GGTGA 3 cut(s) 199, 439, 468
Hpy188I TCNGA 1 cut(s) 328
Hpy188III TCNNGA 1 cut(s) 92
Hpy99I CGWCG 2 cut(s) 36, 119
HpyAV CCTTC 1 cut(s) 417
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4V TGCA 3 cut(s) 136, 239, 419
HpyF3I CTNAG 1 cut(s) 336
HspAI GCGC 1 cut(s) 99
Kzo9I GATC 2 cut(s) 88, 94
MabI ACCWGGT 1 cut(s) 392
MaeIII GTNAC 1 cut(s) 404
MalI GATC 2 cut(s) 90, 96
MbiI CCGCTC 1 cut(s) 143
MboI GATC 2 cut(s) 88, 94
MboII GAAGA 3 cut(s) 96, 136, 452
MfeI CAATTG 2 cut(s) 240, 414
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 7 cut(s) 39, 179, 240, 251, 311, 414, 472
MlyI GAGTC 1 cut(s) 369
MnlI CCTC 5 cut(s) 83, 112, 163, 178, 322
MslI CAYNNNNRTG 2 cut(s) 25, 227
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 3 cut(s) 211, 394, 480
MunI CAATTG 2 cut(s) 240, 414
MvaI CCWGG 3 cut(s) 211, 394, 480
MvnI CGCG 1 cut(s) 99
NdeI CATATG 1 cut(s) 370
NdeII GATC 2 cut(s) 88, 94
NmuCI GTSAC 1 cut(s) 404
NspV TTCGAA 1 cut(s) 82
OliI CACNNNNGTG 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 88
PfeI GAWTC 1 cut(s) 270
PleI GAGTC 1 cut(s) 368
PpsI GAGTC 1 cut(s) 368
Psp6I CCWGG 3 cut(s) 209, 392, 478
PspGI CCWGG 3 cut(s) 209, 392, 478
RsaI GTAC 3 cut(s) 149, 200, 347
RsaNI GTAC 3 cut(s) 148, 199, 346
RseI CAYNNNNRTG 2 cut(s) 25, 227
Sau3AI GATC 2 cut(s) 88, 94
ScaI AGTACT 1 cut(s) 200
SchI GAGTC 1 cut(s) 369
ScrFI CCNGG 3 cut(s) 211, 394, 480
SduI GDGCHC 1 cut(s) 289
SetI ASST 7 cut(s) 111, 159, 333, 337, 347, 395, 481
SexAI ACCWGGT 1 cut(s) 392
SfuI TTCGAA 1 cut(s) 82
SmiMI CAYNNNNRTG 2 cut(s) 25, 227
Sse9I AATT 7 cut(s) 39, 179, 240, 251, 311, 414, 472
SsiI CCGC 1 cut(s) 143
StyD4I CCNGG 3 cut(s) 209, 392, 478
TaaI ACNGT 1 cut(s) 404
TaqI TCGA 3 cut(s) 82, 114, 399
TasI AATT 7 cut(s) 39, 179, 240, 251, 311, 414, 472
TatI WGTACW 1 cut(s) 198
TfiI GAWTC 1 cut(s) 270
TseFI GTSAC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 404
XapI RAATTY 2 cut(s) 39, 251
ZrmI AGTACT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.