Rh4DG259400

Belongs to the peptidase M18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017800
Physical Location & Seq
Reverse (-)
47643855 .. 47647109
3255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG259400.1

Sequence Viewer

Length: 519 bp
ATGTGTGGTTATGACGAGGCAAAGAAGCGTATGCAAAGAGCGGGGTATGAGCAGGTTTTGGAGAGAGAGGATTGGAAATTGGAGGCTGGTAAAAAGTACTACTTCACCAGGAACTACTCCACCATTGTGGCTTTTGCTATCGGTAAAAAATTTGTTGACGAAAATGGATTCCATATAGTTGGTGCTCATACTGATAGTCCTTGCCTTAAATTGAAGCCTGTGTCTAAGGTAGCTAAGGGTGGGTACTTGGAAGTGGGAGTCCAAACATATGGAGGTGGCTTGTGGCATACCTGGTTCGACTGTGACTTGACAATTGCAGGAAGGGTGATAGTAAGACAAGAGAAAGATGGTGTTGTTTCTTATTCACATAGACTTGTCCGGATTGAGGATCCCATCATGCGGATTCCTACTCTGACAATTCACCTGGACAGGGATGTTAGAGAAGCGTTTAAGGTGAATGCACAGAGTCACCTTCTTCCTGTCTTGGCAACCGCAGTCAAGAGGGGAGGGCTATGTTGA

Protein Analysis

172

Amino Acids

19.46

Weight (kDa)

9.14

Isoelectric Point (pI)

28.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M18 PF02127 6 - 166 1.1e-57 Aminopeptidase I zinc metalloprotease (M18)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017800)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0425041 RchiOBHm_Chr7g0233781
rosa_laevigata RLG00000000804 RLG00000015399
rosa_roxburghii Rroxscaffold_2G00092620
rosa_samantha Rh4CG034400 Rh4DG259400 Rh5AG471200 Rh6AG020500 Rh6BG016300 Rh7CG458700
rosa_wichuraiana Rw4G022290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 43
AccBSI CCGCTC 1 cut(s) 41
AccIII TCCGGA 1 cut(s) 378
AciI CCGC 3 cut(s) 41, 400, 492
AclWI GGATC 2 cut(s) 383, 396
AcsI RAATTY 1 cut(s) 149
AfaI GTAC 2 cut(s) 98, 245
AfiI CCNNNNNNNGG 3 cut(s) 385, 399, 430
AgsI TTSAA 1 cut(s) 214
AjnI CCWGG 3 cut(s) 107, 290, 423
AleI CACNNNNGTG 1 cut(s) 125
AluBI AGCT 1 cut(s) 233
AluI AGCT 1 cut(s) 233
Alw21I GWGCWC 1 cut(s) 187
AlwI GGATC 2 cut(s) 383, 396
Aor13HI TCCGGA 1 cut(s) 378
ApoI RAATTY 1 cut(s) 149
AsuHPI GGTGA 5 cut(s) 97, 337, 413, 461, 466
BamHI GGATCC 1 cut(s) 388
Bbv12I GWGCWC 1 cut(s) 187
BccI CCATC 2 cut(s) 341, 401
BciT130I CCWGG 3 cut(s) 109, 292, 425
BfuAI ACCTGC 1 cut(s) 43
BmcAI AGTACT 1 cut(s) 98
Bme1390I CCNGG 3 cut(s) 109, 292, 425
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 3 cut(s) 109, 292, 425
Bpu10I CCTNAGC 1 cut(s) 234
BsaWI WCCGGW 1 cut(s) 378
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 399, 430
BseAI TCCGGA 1 cut(s) 378
BseBI CCWGG 3 cut(s) 109, 292, 425
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 3 cut(s) 385, 399, 430
BsiHKAI GWGCWC 1 cut(s) 187
BsiSI CCGG 1 cut(s) 379
BslI CCNNNNNNNGG 3 cut(s) 385, 399, 430
BsmI GAATGC 1 cut(s) 463
Bsp1286I GDGCHC 1 cut(s) 187
Bsp13I TCCGGA 1 cut(s) 378
Bsp143I GATC 1 cut(s) 388
BspACI CCGC 3 cut(s) 41, 400, 492
BspEI TCCGGA 1 cut(s) 378
BspLI GGNNCC 1 cut(s) 390
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 2 cut(s) 383, 396
BsrBI CCGCTC 1 cut(s) 41
BssMI GATC 1 cut(s) 388
Bst2UI CCWGG 3 cut(s) 109, 292, 425
Bst4CI ACNGT 1 cut(s) 302
BstDEI CTNAG 2 cut(s) 225, 234
BstF5I GGATG 1 cut(s) 439
BstKTI GATC 1 cut(s) 391
BstMBI GATC 1 cut(s) 388
BstNI CCWGG 3 cut(s) 109, 292, 425
BstSCI CCNGG 3 cut(s) 107, 290, 423
BstX2I RGATCY 1 cut(s) 388
BstXI CCANNNNNNTGG 3 cut(s) 127, 179, 269
BstYI RGATCY 1 cut(s) 388
BtsCI GGATG 1 cut(s) 439
BveI ACCTGC 1 cut(s) 43
CsiI ACCWGGT 1 cut(s) 290
Csp6I GTAC 2 cut(s) 97, 244
CviAII CATG 1 cut(s) 397
CviJI RGCY 6 cut(s) 86, 131, 217, 233, 279, 511
CviKI_1 RGCY 6 cut(s) 86, 131, 217, 233, 279, 511
CviQI GTAC 2 cut(s) 97, 244
DdeI CTNAG 2 cut(s) 225, 234
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
EcoRII CCWGG 3 cut(s) 107, 290, 423
FaeI CATG 1 cut(s) 400
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FatI CATG 1 cut(s) 396
FauI CCCGC 1 cut(s) 34
FauNDI CATATG 1 cut(s) 268
FokI GGATG 1 cut(s) 446
HapII CCGG 1 cut(s) 379
Hin1II CATG 1 cut(s) 400
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HinfI GANTC 4 cut(s) 168, 258, 403, 466
HpaII CCGG 1 cut(s) 379
HphI GGTGA 5 cut(s) 97, 337, 413, 461, 466
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 1 cut(s) 414
Hpy188III TCNNGA 2 cut(s) 379, 499
Hpy8I GTNNAC 1 cut(s) 157
HpyAV CCTTC 2 cut(s) 315, 482
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4V TGCA 3 cut(s) 34, 317, 461
HpyF3I CTNAG 2 cut(s) 225, 234
Hsp92II CATG 1 cut(s) 400
Kpn2I TCCGGA 1 cut(s) 378
Kzo9I GATC 1 cut(s) 388
MabI ACCWGGT 1 cut(s) 290
MaeIII GTNAC 2 cut(s) 302, 467
MalI GATC 1 cut(s) 390
MbiI CCGCTC 1 cut(s) 41
MboI GATC 1 cut(s) 388
MboII GAAGA 1 cut(s) 467
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 187
MluCI AATT 5 cut(s) 77, 149, 209, 312, 417
MlyI GAGTC 2 cut(s) 267, 475
MnlI CCTC 7 cut(s) 10, 61, 76, 266, 379, 495, 500
MroI TCCGGA 1 cut(s) 378
MseI TTAA 2 cut(s) 207, 450
MslI CAYNNNNRTG 1 cut(s) 125
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 3 cut(s) 109, 292, 425
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 463
MvaI CCWGG 3 cut(s) 109, 292, 425
NdeI CATATG 1 cut(s) 268
NdeII GATC 1 cut(s) 388
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 2 cut(s) 302, 467
OliI CACNNNNGTG 1 cut(s) 125
PctI GAATGC 1 cut(s) 463
PfeI GAWTC 2 cut(s) 168, 403
PleI GAGTC 2 cut(s) 266, 474
PpsI GAGTC 2 cut(s) 266, 474
Psp6I CCWGG 3 cut(s) 107, 290, 423
PspGI CCWGG 3 cut(s) 107, 290, 423
PspN4I GGNNCC 1 cut(s) 390
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 2 cut(s) 98, 245
RsaNI GTAC 2 cut(s) 97, 244
RseI CAYNNNNRTG 1 cut(s) 125
SaqAI TTAA 2 cut(s) 207, 450
Sau3AI GATC 1 cut(s) 388
ScaI AGTACT 1 cut(s) 98
SchI GAGTC 2 cut(s) 267, 475
ScrFI CCNGG 3 cut(s) 109, 292, 425
SduI GDGCHC 1 cut(s) 187
SetI ASST 8 cut(s) 57, 231, 235, 277, 293, 426, 456, 474
SexAI ACCWGGT 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 125
Sse9I AATT 5 cut(s) 77, 149, 209, 312, 417
SsiI CCGC 3 cut(s) 41, 400, 492
StyD4I CCNGG 3 cut(s) 107, 290, 423
TaaI ACNGT 1 cut(s) 302
TaqI TCGA 1 cut(s) 297
TasI AATT 5 cut(s) 77, 149, 209, 312, 417
TatI WGTACW 1 cut(s) 96
TfiI GAWTC 2 cut(s) 168, 403
Tru1I TTAA 2 cut(s) 207, 450
Tru9I TTAA 2 cut(s) 207, 450
TseFI GTSAC 2 cut(s) 302, 467
Tsp45I GTSAC 2 cut(s) 302, 467
XapI RAATTY 1 cut(s) 149
ZrmI AGTACT 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.