Rw4G022290

Belongs to the peptidase M18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017800
Physical Location & Seq
Reverse (-)
47590504 .. 47592022
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G022290.1

Sequence Viewer

Length: 435 bp
ATGTGTGGTTATGACGAGGCAAAGAAGCGTATGCAAAGAGCGGGGTATGAGCAGGTTTTGGAGAGAGAGGATTGGAAATTGGAGGCTGGTAAAAAGTACTACTTCACCAGGAACTACTCCACCATTGTGGCTTTTGCTATCGGTAAAAAATTTGTTGCCGAAAATGGATTCCATATAGTTGGTGCTCATACTGATAGTCCTTGCCTTAAATTGAAGCCTGTGTCCAAGGTAGCTAAGGGTGGGTACTTGGAAGTGGGAGTCCAAACATATGGAGGTGGCTTGTGGCATACCTGGTTCGACTGTGACTTGACAATTGCAGGAAGGGTGATAGTAAGACAAGAGAAAGATGGTGTTGTTTCTTATTCACATAGACTTGTCCGGATTGAGGATCCCATCATGCGGATTCCTACTCTGACAATTCACCTGGACAGGTAA

Protein Analysis

144

Amino Acids

16.44

Weight (kDa)

9.11

Isoelectric Point (pI)

26.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M18 PF02127 6 - 144 6.1e-52 Aminopeptidase I zinc metalloprotease (M18)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017800)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0425041 RchiOBHm_Chr7g0233781
rosa_laevigata RLG00000000804 RLG00000015399
rosa_roxburghii Rroxscaffold_2G00092620
rosa_samantha Rh4CG034400 Rh4DG259400 Rh5AG471200 Rh6AG020500 Rh6BG016300 Rh7CG458700
rosa_wichuraiana Rw4G022290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 43
AccBSI CCGCTC 1 cut(s) 41
AccIII TCCGGA 1 cut(s) 378
AciI CCGC 2 cut(s) 41, 400
AclWI GGATC 2 cut(s) 383, 396
AcsI RAATTY 1 cut(s) 149
AfaI GTAC 2 cut(s) 98, 245
AfiI CCNNNNNNNGG 2 cut(s) 385, 399
AgsI TTSAA 1 cut(s) 214
AjnI CCWGG 3 cut(s) 107, 290, 423
AleI CACNNNNGTG 1 cut(s) 125
AluBI AGCT 1 cut(s) 233
AluI AGCT 1 cut(s) 233
Alw21I GWGCWC 1 cut(s) 187
AlwI GGATC 2 cut(s) 383, 396
Aor13HI TCCGGA 1 cut(s) 378
ApoI RAATTY 1 cut(s) 149
AsuHPI GGTGA 3 cut(s) 97, 337, 413
BamHI GGATCC 1 cut(s) 388
Bbv12I GWGCWC 1 cut(s) 187
BccI CCATC 2 cut(s) 341, 401
BciT130I CCWGG 3 cut(s) 109, 292, 425
BfuAI ACCTGC 1 cut(s) 43
BmcAI AGTACT 1 cut(s) 98
Bme1390I CCNGG 3 cut(s) 109, 292, 425
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 3 cut(s) 109, 292, 425
Bpu10I CCTNAGC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 225
BsaWI WCCGGW 1 cut(s) 378
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 399
BseAI TCCGGA 1 cut(s) 378
BseBI CCWGG 3 cut(s) 109, 292, 425
BseDI CCNNGG 1 cut(s) 225
BseLI CCNNNNNNNGG 2 cut(s) 385, 399
BsiHKAI GWGCWC 1 cut(s) 187
BsiSI CCGG 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 385, 399
Bsp1286I GDGCHC 1 cut(s) 187
Bsp13I TCCGGA 1 cut(s) 378
Bsp143I GATC 1 cut(s) 388
BspACI CCGC 2 cut(s) 41, 400
BspEI TCCGGA 1 cut(s) 378
BspLI GGNNCC 1 cut(s) 390
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 2 cut(s) 383, 396
BsrBI CCGCTC 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 225
BssMI GATC 1 cut(s) 388
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 3 cut(s) 109, 292, 425
Bst4CI ACNGT 1 cut(s) 302
BstDEI CTNAG 1 cut(s) 234
BstKTI GATC 1 cut(s) 391
BstMBI GATC 1 cut(s) 388
BstNI CCWGG 3 cut(s) 109, 292, 425
BstSCI CCNGG 3 cut(s) 107, 290, 423
BstX2I RGATCY 1 cut(s) 388
BstXI CCANNNNNNTGG 3 cut(s) 127, 179, 269
BstYI RGATCY 1 cut(s) 388
BveI ACCTGC 1 cut(s) 43
CsiI ACCWGGT 1 cut(s) 290
Csp6I GTAC 2 cut(s) 97, 244
CviAII CATG 1 cut(s) 397
CviJI RGCY 5 cut(s) 86, 131, 217, 233, 279
CviKI_1 RGCY 5 cut(s) 86, 131, 217, 233, 279
CviQI GTAC 2 cut(s) 97, 244
DdeI CTNAG 1 cut(s) 234
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
Eco130I CCWWGG 1 cut(s) 225
EcoRII CCWGG 3 cut(s) 107, 290, 423
EcoT14I CCWWGG 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 1 cut(s) 400
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FatI CATG 1 cut(s) 396
FauI CCCGC 1 cut(s) 34
FauNDI CATATG 1 cut(s) 268
HapII CCGG 1 cut(s) 379
Hin1II CATG 1 cut(s) 400
HinfI GANTC 3 cut(s) 168, 258, 403
HpaII CCGG 1 cut(s) 379
HphI GGTGA 3 cut(s) 97, 337, 413
Hpy188I TCNGA 1 cut(s) 414
Hpy188III TCNNGA 1 cut(s) 379
HpyAV CCTTC 1 cut(s) 315
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4V TGCA 2 cut(s) 34, 317
HpyF3I CTNAG 1 cut(s) 234
Hsp92II CATG 1 cut(s) 400
Kpn2I TCCGGA 1 cut(s) 378
Kzo9I GATC 1 cut(s) 388
MabI ACCWGGT 1 cut(s) 290
MaeIII GTNAC 1 cut(s) 302
MalI GATC 1 cut(s) 390
MbiI CCGCTC 1 cut(s) 41
MboI GATC 1 cut(s) 388
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 187
MluCI AATT 5 cut(s) 77, 149, 209, 312, 417
MlyI GAGTC 1 cut(s) 267
MnlI CCTC 5 cut(s) 10, 61, 76, 266, 379
MroI TCCGGA 1 cut(s) 378
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 1 cut(s) 125
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 3 cut(s) 109, 292, 425
MunI CAATTG 1 cut(s) 312
MvaI CCWGG 3 cut(s) 109, 292, 425
NdeI CATATG 1 cut(s) 268
NdeII GATC 1 cut(s) 388
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 302
OliI CACNNNNGTG 1 cut(s) 125
PfeI GAWTC 2 cut(s) 168, 403
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
Psp6I CCWGG 3 cut(s) 107, 290, 423
PspGI CCWGG 3 cut(s) 107, 290, 423
PspN4I GGNNCC 1 cut(s) 390
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 2 cut(s) 98, 245
RsaNI GTAC 2 cut(s) 97, 244
RseI CAYNNNNRTG 1 cut(s) 125
SaqAI TTAA 1 cut(s) 207
Sau3AI GATC 1 cut(s) 388
ScaI AGTACT 1 cut(s) 98
SchI GAGTC 1 cut(s) 267
ScrFI CCNGG 3 cut(s) 109, 292, 425
SduI GDGCHC 1 cut(s) 187
SetI ASST 7 cut(s) 57, 231, 235, 277, 293, 426, 434
SexAI ACCWGGT 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 125
Sse9I AATT 5 cut(s) 77, 149, 209, 312, 417
SsiI CCGC 2 cut(s) 41, 400
StyD4I CCNGG 3 cut(s) 107, 290, 423
StyI CCWWGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 302
TaqI TCGA 1 cut(s) 297
TasI AATT 5 cut(s) 77, 149, 209, 312, 417
TatI WGTACW 1 cut(s) 96
TfiI GAWTC 2 cut(s) 168, 403
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TseFI GTSAC 1 cut(s) 302
Tsp45I GTSAC 1 cut(s) 302
XapI RAATTY 1 cut(s) 149
ZrmI AGTACT 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.