Rh5AG471200

Belongs to the peptidase M18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0017800
Physical Location & Seq
Reverse (-)
80790764 .. 80793313
2550 bp
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UTR
Exon/CDS
Intron
Rh5AG471200.1

Sequence Viewer

Length: 783 bp
ATGGACCTGACTTATAGGAGACTCAAGAAGAGGATCCCGCCAGGGATTCAACGATGGCTTCAACAATATTCTTTGATCTTTTCTGAGGATCTGACTCCAAGCAACATCAAGAACATAGTTTTGACAGAGATCCTCCAATCACTTTCTTTGACCTTCCCGAACTTTGCAGAACATCACCATAAATTCATTGATTTTCCGAGCTTTGCGCGCATTGGCACATTGTTCTACTGCCTTGCCAAGCTTGGGAAAATCAAAGATCGTGATCGCGCTTTCCACCTTATCGACGAGGCGAAGAAGCGTCTGCAAAGAGCGGGGTACGAGCAGGTTTTGGAGAGAGAGGATTGGAAATTGGAGGCTGGTAAAAAGTACTACTTCACCAGGAACTACTCCACCATTGTGGCTTTTGCAATTGGTAAAAAATTTGTTGCCGGAAATGGATTCCATATAGTTGGTGCTCATACTAATAGTCCTTGTCTCAAATTGAAGCCTGTTTCCAAGGTAGCTAAGGGTAGGTACTTGGAAGTGGGAGTCCAAACATATGGAGATGGCTTGTGGCATACCTGGTTCGACCGTGACTTGACAATTGCAGGAAGGGTGATCGTAAGAGAAGAGAAAGATGGTGTTGTTTCTTACTCACATAGACTTGTTCGGATTGAGGAGCCCATCATGCGGATTCCTACTGTGGCAATTCACCTGGACAGGGATGTTAGAGAGGCGTTTAAGGTGAATGCACAGAGTCACCTTCTTCCTGTCTTGGCAACAACAGTCAAGGTAATCTTGTAA

Protein Analysis

260

Amino Acids

30.03

Weight (kDa)

9.75

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M18 PF02127 89 - 255 2.8e-58 Aminopeptidase I zinc metalloprotease (M18)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017800)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0425041 RchiOBHm_Chr7g0233781
rosa_laevigata RLG00000000804 RLG00000015399
rosa_roxburghii Rroxscaffold_2G00092620
rosa_samantha Rh4CG034400 Rh4DG259400 Rh5AG471200 Rh6AG020500 Rh6BG016300 Rh7CG458700
rosa_wichuraiana Rw4G022290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccBSI CCGCTC 1 cut(s) 311
AccII CGCG 2 cut(s) 208, 267
AciI CCGC 3 cut(s) 38, 311, 670
AclWI GGATC 4 cut(s) 28, 41, 96, 124
AcsI RAATTY 2 cut(s) 182, 419
AfaI GTAC 3 cut(s) 317, 368, 515
AfiI CCNNNNNNNGG 3 cut(s) 243, 669, 700
AgsI TTSAA 3 cut(s) 50, 62, 484
AjnI CCWGG 4 cut(s) 40, 377, 560, 693
AleI CACNNNNGTG 1 cut(s) 395
AluBI AGCT 3 cut(s) 201, 241, 503
AluI AGCT 3 cut(s) 201, 241, 503
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 2 cut(s) 13, 479
AlwI GGATC 4 cut(s) 28, 41, 96, 124
ApoI RAATTY 2 cut(s) 182, 419
AspLEI GCGC 3 cut(s) 208, 210, 269
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 6 cut(s) 167, 367, 607, 683, 731, 736
AvaII GGWCC 1 cut(s) 4
BamHI GGATCC 1 cut(s) 33
BanII GRGCYC 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 457
BccI CCATC 4 cut(s) 48, 539, 611, 671
BciT130I CCWGG 4 cut(s) 42, 379, 562, 695
BcoDI GTCTC 2 cut(s) 13, 479
BfuAI ACCTGC 1 cut(s) 313
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 4 cut(s) 42, 379, 562, 695
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 35, 660
BmrFI CCNGG 4 cut(s) 42, 379, 562, 695
Bpu10I CCTNAGC 1 cut(s) 504
BpuEI CTTGAG 1 cut(s) 8
BsaBI GATNNNNATC 1 cut(s) 261
BsaJI CCNNGG 2 cut(s) 41, 495
Bsc4I CCNNNNNNNGG 3 cut(s) 243, 669, 700
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 4 cut(s) 42, 379, 562, 695
BseDI CCNNGG 2 cut(s) 41, 495
BseGI GGATG 1 cut(s) 709
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 3 cut(s) 243, 669, 700
BseMII CTCAG 1 cut(s) 75
BsePI GCGCGC 1 cut(s) 206
BseRI GAGGAG 1 cut(s) 671
Bsh1236I CGCG 2 cut(s) 208, 267
Bsh1285I CGRYCG 1 cut(s) 571
BsiEI CGRYCG 1 cut(s) 571
BsiHKAI GWGCWC 1 cut(s) 457
BsiSI CCGG 1 cut(s) 429
BslI CCNNNNNNNGG 3 cut(s) 243, 669, 700
BsmAI GTCTC 2 cut(s) 13, 479
BsmI GAATGC 1 cut(s) 733
Bsp1286I GDGCHC 2 cut(s) 457, 663
Bsp143I GATC 7 cut(s) 33, 75, 88, 129, 256, 262, 597
BspACI CCGC 3 cut(s) 38, 311, 670
BspCNI CTCAG 1 cut(s) 76
BspFNI CGCG 2 cut(s) 208, 267
BspLI GGNNCC 2 cut(s) 35, 660
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 4 cut(s) 28, 41, 96, 124
BsrBI CCGCTC 1 cut(s) 311
BssECI CCNNGG 2 cut(s) 41, 495
BssHII GCGCGC 1 cut(s) 206
BssMI GATC 7 cut(s) 33, 75, 88, 129, 256, 262, 597
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 4 cut(s) 42, 379, 562, 695
Bst4CI ACNGT 3 cut(s) 572, 682, 766
Bst6I CTCTTC 2 cut(s) 23, 603
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 2 cut(s) 84, 504
BstF5I GGATG 1 cut(s) 709
BstFNI CGCG 2 cut(s) 208, 267
BstHHI GCGC 3 cut(s) 208, 210, 269
BstKTI GATC 7 cut(s) 36, 78, 91, 132, 259, 265, 600
BstMAI GTCTC 2 cut(s) 13, 479
BstMBI GATC 7 cut(s) 33, 75, 88, 129, 256, 262, 597
BstMCI CGRYCG 1 cut(s) 571
BstMWI GCNNNNNNNGC 2 cut(s) 207, 667
BstNI CCWGG 4 cut(s) 42, 379, 562, 695
BstSCI CCNGG 4 cut(s) 40, 377, 560, 693
BstUI CGCG 2 cut(s) 208, 267
BstX2I RGATCY 3 cut(s) 33, 88, 129
BstXI CCANNNNNNTGG 3 cut(s) 397, 449, 539
BstYI RGATCY 3 cut(s) 33, 88, 129
BtsCI GGATG 1 cut(s) 709
BveI ACCTGC 1 cut(s) 313
Cac8I GCNNGC 1 cut(s) 208
CfoI GCGC 3 cut(s) 208, 210, 269
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 287
CsiI ACCWGGT 1 cut(s) 560
Csp6I GTAC 3 cut(s) 316, 367, 514
CviAII CATG 1 cut(s) 667
CviJI RGCY 9 cut(s) 58, 201, 241, 356, 401, 487, 503, 549, 661
CviKI_1 RGCY 9 cut(s) 58, 201, 241, 356, 401, 487, 503, 549, 661
CviQI GTAC 3 cut(s) 316, 367, 514
DdeI CTNAG 2 cut(s) 84, 504
DpnI GATC 7 cut(s) 35, 77, 90, 131, 258, 264, 599
DpnII GATC 7 cut(s) 33, 75, 88, 129, 256, 262, 597
Eam1104I CTCTTC 2 cut(s) 23, 603
EarI CTCTTC 2 cut(s) 23, 603
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 663
Eco47I GGWCC 1 cut(s) 4
EcoRII CCWGG 4 cut(s) 40, 377, 560, 693
EcoT14I CCWWGG 1 cut(s) 495
EcoT38I GRGCYC 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 1 cut(s) 670
FalI AAGNNNNNCTT 3 cut(s) 356, 388, 761
FatI CATG 1 cut(s) 666
FauI CCCGC 2 cut(s) 45, 304
FauNDI CATATG 1 cut(s) 538
FokI GGATG 1 cut(s) 716
FriOI GRGCYC 1 cut(s) 663
GlaI GCGC 3 cut(s) 207, 209, 268
HapII CCGG 1 cut(s) 429
HgaI GACGC 1 cut(s) 287
HhaI GCGC 3 cut(s) 208, 210, 269
Hin1II CATG 1 cut(s) 670
Hin6I GCGC 3 cut(s) 206, 208, 267
HinP1I GCGC 3 cut(s) 206, 208, 267
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 7 cut(s) 21, 46, 94, 438, 528, 673, 736
HpaII CCGG 1 cut(s) 429
HphI GGTGA 6 cut(s) 167, 367, 607, 683, 731, 736
Hpy188I TCNGA 4 cut(s) 85, 93, 198, 651
Hpy188III TCNNGA 4 cut(s) 25, 109, 157, 260
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 3 cut(s) 163, 585, 752
HpyCH4III ACNGT 3 cut(s) 572, 682, 766
HpyCH4V TGCA 5 cut(s) 167, 304, 407, 587, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 207, 667
HpyF3I CTNAG 2 cut(s) 84, 504
Hsp92II CATG 1 cut(s) 670
HspAI GCGC 3 cut(s) 206, 208, 267
Kzo9I GATC 7 cut(s) 33, 75, 88, 129, 256, 262, 597
LmnI GCTCC 1 cut(s) 658
MabI ACCWGGT 1 cut(s) 560
MaeIII GTNAC 2 cut(s) 572, 737
MalI GATC 7 cut(s) 35, 77, 90, 131, 258, 264, 599
MbiI CCGCTC 1 cut(s) 311
MboI GATC 7 cut(s) 33, 75, 88, 129, 256, 262, 597
MboII GAAGA 4 cut(s) 40, 304, 620, 737
MfeI CAATTG 2 cut(s) 408, 582
MflI RGATCY 3 cut(s) 33, 88, 129
MhlI GDGCHC 2 cut(s) 457, 663
MluCI AATT 7 cut(s) 182, 347, 408, 419, 479, 582, 687
MlyI GAGTC 4 cut(s) 15, 88, 537, 745
MnlI CCTC 8 cut(s) 24, 79, 143, 280, 331, 346, 649, 706
MseI TTAA 1 cut(s) 720
MslI CAYNNNNRTG 1 cut(s) 395
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 4 cut(s) 42, 379, 562, 695
MunI CAATTG 2 cut(s) 408, 582
Mva1269I GAATGC 1 cut(s) 733
MvaI CCWGG 4 cut(s) 42, 379, 562, 695
MvnI CGCG 2 cut(s) 208, 267
MwoI GCNNNNNNNGC 2 cut(s) 207, 667
NdeI CATATG 1 cut(s) 538
NdeII GATC 7 cut(s) 33, 75, 88, 129, 256, 262, 597
NlaIII CATG 1 cut(s) 670
NlaIV GGNNCC 2 cut(s) 35, 660
NmuCI GTSAC 2 cut(s) 572, 737
OliI CACNNNNGTG 1 cut(s) 395
PauI GCGCGC 1 cut(s) 206
PctI GAATGC 1 cut(s) 733
PfeI GAWTC 3 cut(s) 46, 438, 673
PleI GAGTC 4 cut(s) 15, 88, 536, 744
PpsI GAGTC 4 cut(s) 15, 88, 536, 744
Psp6I CCWGG 4 cut(s) 40, 377, 560, 693
PspGI CCWGG 4 cut(s) 40, 377, 560, 693
PspN4I GGNNCC 2 cut(s) 35, 660
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 3 cut(s) 33, 88, 129
PteI GCGCGC 1 cut(s) 206
RsaI GTAC 3 cut(s) 317, 368, 515
RsaNI GTAC 3 cut(s) 316, 367, 514
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 1 cut(s) 720
Sau3AI GATC 7 cut(s) 33, 75, 88, 129, 256, 262, 597
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 4 cut(s) 15, 88, 537, 745
ScrFI CCNGG 4 cut(s) 42, 379, 562, 695
SduI GDGCHC 2 cut(s) 457, 663
SexAI ACCWGGT 1 cut(s) 560
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
Sse9I AATT 7 cut(s) 182, 347, 408, 419, 479, 582, 687
SsiI CCGC 3 cut(s) 38, 311, 670
SspI AATATT 1 cut(s) 68
StyD4I CCNGG 4 cut(s) 40, 377, 560, 693
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 3 cut(s) 572, 682, 766
TaqI TCGA 2 cut(s) 282, 567
TasI AATT 7 cut(s) 182, 347, 408, 419, 479, 582, 687
TatI WGTACW 1 cut(s) 366
TfiI GAWTC 3 cut(s) 46, 438, 673
Tru1I TTAA 1 cut(s) 720
Tru9I TTAA 1 cut(s) 720
TseFI GTSAC 2 cut(s) 572, 737
Tsp45I GTSAC 2 cut(s) 572, 737
TspDTI ATGAA 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 2 cut(s) 182, 419
ZrmI AGTACT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.