RLG00000001480
MADS Family

Agamous-like MADS-box protein AGL12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012166
Physical Location & Seq
Reverse (-)
16174203 .. 16176598
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001480

Sequence Viewer

Length: 636 bp
ATGGCTCGTGGAAAGGTTCAGATGAAGAAGATCGAGAACCCGGTGCACCGACAGGTTACCTTCTGCAAGCGCCGAGCAGGGCTTCTGAAGAAGGCCAAGGAGCTTTCTGTGCTGTGTGATGCAGATATTGGAGTTCTTATTTACTCCTCCCATGGAAAGCTCTTTGAACTAGCCACCAAAGGAACCATGCAAGGGCTTATTGAAAGGTACATGAAAATGAAGCCTGCTCGAGTACCTCAGGCTGAAACAGCCATGGAAACCCAGACTCTGGATGCAAAAAAGGAGATTAACTTGCTGAAACAAGAGATTGAAATACTACAAAAAGGTCTCAGGTATATGTTTGGAGGAGGTGCTGGGACAATGTCTTTGGATGAACTACAAGTGCTTGAAAAACATCTTGAACTTTGGATTTACCACGTGCGCTCGGCAAAGATGGACATTTTGCTCCAAGAGATTGAGATGTTGAGGAATTCAGAAGGAATACTGACAGCTGCAAATAAGTATCTTCAAGACAAGATTGAAGAGAACAGTGGAGTCACTAACCTAATACCAATGGCCTCTGCTAGCCCATATCCACTAACCATACCAAATGATGACATAATTTCAATCCTGGGAAGCAACTTTGGCTTGAATTAG

Protein Analysis

212

Amino Acids

23.68

Weight (kDa)

8.74

Isoelectric Point (pI)

60.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 56 4.3e-23 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 88 - 174 2.2e-21 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012166)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71692
fragaria_vesca FvH4_5g32850 FvH4_5g32850
malus_domestica MD08G1216000.v1.1 MD15G1400400.v1.1
prunus_persica Prupe.1G548000_v2.0.a1 Prupe.1G548000_v2.0.a1
pyrus_communis pycom15g35900
rosa_chinensis RchiOBHm_Chr7g0230721
rosa_laevigata RLG00000001480
rosa_multiflora Rmu_sc0007883.1_g000004
rosa_roxburghii Rroxscaffold_3G00229810
rosa_rugosa Rorug07G0262100 Rorug07G0262200 Rorug07G0262300
rosa_samantha Rh7AG414000 Rh7BG391000 Rh7CG432900
rosa_wichuraiana Rw7G034370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 268
AcsI RAATTY 1 cut(s) 469
AcuI CTGAAG 1 cut(s) 107
AcvI CACGTG 1 cut(s) 418
AfaI GTAC 2 cut(s) 209, 234
AfiI CCNNNNNNNGG 2 cut(s) 192, 268
AgsI TTSAA 9 cut(s) 167, 203, 311, 389, 401, 509, 521, 606, 631
AjnI CCWGG 1 cut(s) 609
AluBI AGCT 3 cut(s) 103, 160, 491
AluI AGCT 3 cut(s) 103, 160, 491
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 1 cut(s) 332
Alw44I GTGCAC 1 cut(s) 44
AlwNI CAGNNNCTG 1 cut(s) 268
Ama87I CYCGRG 1 cut(s) 228
AoxI GGCC 2 cut(s) 93, 555
ApaLI GTGCAC 1 cut(s) 44
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 1 cut(s) 469
ArsI GACNNNNNNTTYG 2 cut(s) 349, 381
AspLEI GCGC 2 cut(s) 72, 423
AsuC2I CCSGG 1 cut(s) 41
AsuNHI GCTAGC 1 cut(s) 563
AvaI CYCGRG 1 cut(s) 228
AxyI CCTNAGG 1 cut(s) 237
BaeGI GKGCMC 1 cut(s) 48
BauI CACGAG 1 cut(s) 6
BbrPI CACGTG 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 1 cut(s) 478
BccI CCATC 1 cut(s) 427
BciT130I CCWGG 1 cut(s) 611
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 2 cut(s) 170, 564
BfoI RGCGCY 1 cut(s) 73
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
Bme1390I CCNGG 2 cut(s) 41, 611
BmeT110I CYCGRG 1 cut(s) 228
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 2 cut(s) 41, 611
BmsI GCATC 2 cut(s) 109, 262
BmtI GCTAGC 1 cut(s) 567
BpuMI CCSGG 1 cut(s) 41
BsaAI YACGTR 1 cut(s) 418
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 4 cut(s) 96, 151, 252, 610
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 268
Bse21I CCTNAGG 1 cut(s) 237
BseBI CCWGG 1 cut(s) 611
BseDI CCNNGG 4 cut(s) 96, 151, 252, 610
BseGI GGATG 2 cut(s) 277, 376
BseLI CCNNNNNNNGG 2 cut(s) 192, 268
BseMII CTCAG 2 cut(s) 251, 343
BseRI GAGGAG 2 cut(s) 136, 360
BseSI GKGCMC 1 cut(s) 48
BseXI GCAGC 1 cut(s) 478
BseYI CCCAGC 1 cut(s) 353
BshFI GGCC 2 cut(s) 95, 557
BsiHKAI GWGCWC 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 228
BsiSI CCGG 1 cut(s) 41
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 2 cut(s) 192, 268
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 2 cut(s) 95, 557
Bso31I GGTCTC 1 cut(s) 332
BsoBI CYCGRG 1 cut(s) 228
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 1 cut(s) 30
Bsp19I CCATGG 2 cut(s) 151, 252
BspANI GGCC 2 cut(s) 95, 557
BspCNI CTCAG 2 cut(s) 250, 342
BspLI GGNNCC 1 cut(s) 184
BspOI GCTAGC 1 cut(s) 567
BspTNI GGTCTC 1 cut(s) 332
BssECI CCNNGG 4 cut(s) 96, 151, 252, 610
BssMI GATC 1 cut(s) 30
BssSI CACGAG 1 cut(s) 6
BssT1I CCWWGG 3 cut(s) 96, 151, 252
Bst2BI CACGAG 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 611
Bst4CI ACNGT 1 cut(s) 530
Bst6I CTCTTC 1 cut(s) 516
BstBAI YACGTR 1 cut(s) 418
BstC8I GCNNGC 3 cut(s) 68, 225, 565
BstDEI CTNAG 2 cut(s) 237, 329
BstDSI CCRYGG 2 cut(s) 151, 252
BstEII GGTNACC 1 cut(s) 55
BstF5I GGATG 2 cut(s) 277, 376
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 2 cut(s) 72, 423
BstKTI GATC 1 cut(s) 33
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 3 cut(s) 109, 248, 624
BstNI CCWGG 1 cut(s) 611
BstPI GGTNACC 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 39, 609
BstSLI GKGCMC 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 478
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 2 cut(s) 95, 557
BtgI CCRYGG 2 cut(s) 151, 252
BtsCI GGATG 2 cut(s) 277, 376
BtsIMutI CAGTG 1 cut(s) 535
Cac8I GCNNGC 3 cut(s) 68, 225, 565
CaiI CAGNNNCTG 1 cut(s) 268
CfoI GCGC 2 cut(s) 72, 423
Csp6I GTAC 2 cut(s) 208, 233
CviAII CATG 4 cut(s) 152, 187, 211, 253
CviQI GTAC 2 cut(s) 208, 233
DdeI CTNAG 2 cut(s) 237, 329
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Eco130I CCWWGG 3 cut(s) 96, 151, 252
Eco31I GGTCTC 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 107
Eco72I CACGTG 1 cut(s) 418
Eco81I CCTNAGG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 228
Eco91I GGTNACC 1 cut(s) 55
EcoO65I GGTNACC 1 cut(s) 55
EcoRI GAATTC 1 cut(s) 469
EcoRII CCWGG 1 cut(s) 609
EcoT14I CCWWGG 3 cut(s) 96, 151, 252
ErhI CCWWGG 3 cut(s) 96, 151, 252
FaeI CATG 4 cut(s) 155, 190, 214, 256
FaiI YATR 9 cut(s) 153, 188, 212, 254, 336, 338, 571, 584, 599
FaqI GGGAC 1 cut(s) 370
FatI CATG 4 cut(s) 151, 186, 210, 252
Fnu4HI GCNGC 1 cut(s) 492
FokI GGATG 2 cut(s) 284, 383
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 2 cut(s) 170, 564
GlaI GCGC 2 cut(s) 71, 422
GluI GCNGC 1 cut(s) 492
GsaI CCCAGC 1 cut(s) 357
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 2 cut(s) 95, 557
HapII CCGG 1 cut(s) 41
HhaI GCGC 2 cut(s) 72, 423
Hin1II CATG 4 cut(s) 155, 190, 214, 256
Hin6I GCGC 2 cut(s) 70, 421
HinP1I GCGC 2 cut(s) 70, 421
HinfI GANTC 2 cut(s) 265, 534
HpaII CCGG 1 cut(s) 41
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 3 cut(s) 21, 87, 475
Hpy188III TCNNGA 4 cut(s) 34, 269, 398, 509
Hpy8I GTNNAC 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 70, 85, 470
HpyCH4III ACNGT 1 cut(s) 530
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 6 cut(s) 46, 66, 122, 190, 275, 494
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 248, 624
HpyF3I CTNAG 2 cut(s) 237, 329
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 4 cut(s) 155, 190, 214, 256
HspAI GCGC 2 cut(s) 70, 421
Kzo9I GATC 1 cut(s) 30
LmnI GCTCC 2 cut(s) 100, 450
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 2 cut(s) 109, 262
MaeI CTAG 2 cut(s) 170, 564
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 2 cut(s) 55, 535
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 5 cut(s) 37, 40, 100, 497, 533
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 3 cut(s) 469, 600, 631
MlyI GAGTC 2 cut(s) 259, 543
MnlI CCTC 6 cut(s) 157, 246, 338, 341, 459, 568
MseI TTAA 1 cut(s) 288
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 2 cut(s) 41, 611
MvaI CCWGG 1 cut(s) 611
MwoI GCNNNNNNNGC 3 cut(s) 109, 248, 624
NciI CCSGG 1 cut(s) 41
NcoI CCATGG 2 cut(s) 151, 252
NdeII GATC 1 cut(s) 30
NheI GCTAGC 1 cut(s) 563
NlaIII CATG 4 cut(s) 155, 190, 214, 256
NlaIV GGNNCC 1 cut(s) 184
NmeAIII GCCGAG 2 cut(s) 98, 404
NmuCI GTSAC 1 cut(s) 535
PaeR7I CTCGAG 1 cut(s) 228
PflFI GACNNNGTC 1 cut(s) 361
PflMI CCANNNNNTGG 1 cut(s) 268
PkrI GCNGC 1 cut(s) 493
PleI GAGTC 2 cut(s) 259, 542
PmaCI CACGTG 1 cut(s) 418
PmlI CACGTG 1 cut(s) 418
PpsI GAGTC 2 cut(s) 259, 542
Ppu21I YACGTR 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 609
PspCI CACGTG 1 cut(s) 418
PspEI GGTNACC 1 cut(s) 55
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 1 cut(s) 609
PspN4I GGNNCC 1 cut(s) 184
PspXI VCTCGAGB 1 cut(s) 228
PstNI CAGNNNCTG 1 cut(s) 268
PsyI GACNNNGTC 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 2 cut(s) 209, 234
RsaNI GTAC 2 cut(s) 208, 233
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 1 cut(s) 30
SchI GAGTC 2 cut(s) 259, 543
ScrFI CCNGG 2 cut(s) 41, 611
SduI GDGCHC 1 cut(s) 48
SfaNI GCATC 2 cut(s) 109, 262
Sfr274I CTCGAG 1 cut(s) 228
SlaI CTCGAG 1 cut(s) 228
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
Sse9I AATT 3 cut(s) 469, 600, 631
SspMI CTAG 2 cut(s) 170, 564
StyD4I CCNGG 2 cut(s) 39, 609
StyI CCWWGG 3 cut(s) 96, 151, 252
TaaI ACNGT 1 cut(s) 530
TaiI ACGT 1 cut(s) 420
TaqI TCGA 2 cut(s) 33, 229
TasI AATT 3 cut(s) 469, 600, 631
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TscAI CASTG 1 cut(s) 535
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 1 cut(s) 535
TspDTI ATGAA 4 cut(s) 38, 227, 233, 387
TspRI CASTG 1 cut(s) 535
Tth111I GACNNNGTC 1 cut(s) 361
Van91I CCANNNNNTGG 1 cut(s) 268
VneI GTGCAC 1 cut(s) 44
XapI RAATTY 1 cut(s) 469
XhoI CTCGAG 1 cut(s) 228
XspI CTAG 2 cut(s) 170, 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.