Rh7BG391000
MADS Family

Agamous-like MADS-box protein AGL12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0012166
Physical Location & Seq
Forward (+)
42972923 .. 42974885
1963 bp
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UTR
Exon/CDS
Intron
Rh7BG391000.1

Sequence Viewer

Length: 450 bp
ATGCAAGGGCTTATTGAAAGGTACATGAAAATGAAGCCTGCTCGAGTACCTCAGGCTGAAACAGCCATGGAAACCCAGACTCTGGATGCAAAAAAGGAGATTAACTTGCTGAAACAAGAGATTGAAATACTACAAAAAGGTCTCAGGTATATGTTTGGAGGAGGTGCTGGGACAATGTCTTTGGATGAACTACAAGTGCTTGAAAAACATCTTGAACTTTGGATTTACCACGTGCGCTCGGCAAAGATGGACATTTTGCTCCAAGAGATTCAGCTGTTGAGGAATTCAGAAGGAATACTGACAGCTGCAAATAAGTATCTTCAAGACAAGATTGAAGAGAACAGTGGAATCACTAACCTTATACCAATGGCCTCTGATAGCCCATATCCACTAACCATACCAAATGATGACATAATTTCAATCCTAGGAAGCAACTTTGGCTTGAATTAG

Protein Analysis

149

Amino Acids

16.82

Weight (kDa)

5.06

Isoelectric Point (pI)

71.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 25 - 112 5.9e-21 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012166)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71692
fragaria_vesca FvH4_5g32850 FvH4_5g32850
malus_domestica MD08G1216000.v1.1 MD15G1400400.v1.1
prunus_persica Prupe.1G548000_v2.0.a1 Prupe.1G548000_v2.0.a1
pyrus_communis pycom15g35900
rosa_chinensis RchiOBHm_Chr7g0230721
rosa_laevigata RLG00000001480
rosa_multiflora Rmu_sc0007883.1_g000004
rosa_roxburghii Rroxscaffold_3G00229810
rosa_rugosa Rorug07G0262100 Rorug07G0262200 Rorug07G0262300
rosa_samantha Rh7AG414000 Rh7BG391000 Rh7CG432900
rosa_wichuraiana Rw7G034370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 82
AcsI RAATTY 1 cut(s) 283
AcvI CACGTG 1 cut(s) 232
AfaI GTAC 2 cut(s) 23, 48
AfiI CCNNNNNNNGG 1 cut(s) 82
AgsI TTSAA 8 cut(s) 17, 125, 203, 215, 323, 335, 420, 445
AluBI AGCT 2 cut(s) 274, 305
AluI AGCT 2 cut(s) 274, 305
Alw26I GTCTC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 82
Ama87I CYCGRG 1 cut(s) 42
AoxI GGCC 1 cut(s) 369
ApeKI GCWGC 1 cut(s) 305
ApoI RAATTY 1 cut(s) 283
ArsI GACNNNNNNTTYG 2 cut(s) 163, 195
AspA2I CCTAGG 1 cut(s) 424
AspLEI GCGC 1 cut(s) 237
AvaI CYCGRG 1 cut(s) 42
AvrII CCTAGG 1 cut(s) 424
AxyI CCTNAGG 1 cut(s) 51
BbrPI CACGTG 1 cut(s) 232
BbvI GCAGC 1 cut(s) 292
BccI CCATC 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 1 cut(s) 425
BisI GCNGC 1 cut(s) 306
BlnI CCTAGG 1 cut(s) 424
BlsI GCNGC 1 cut(s) 307
BmeT110I CYCGRG 1 cut(s) 42
BmsI GCATC 1 cut(s) 76
BsaAI YACGTR 1 cut(s) 232
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 66, 424
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse21I CCTNAGG 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 66, 424
BseGI GGATG 2 cut(s) 91, 190
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMII CTCAG 2 cut(s) 65, 157
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 1 cut(s) 292
BseYI CCCAGC 1 cut(s) 167
BshFI GGCC 1 cut(s) 371
BsiHKCI CYCGRG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 1 cut(s) 146
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 371
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 42
Bsp19I CCATGG 1 cut(s) 66
BspANI GGCC 1 cut(s) 371
BspCNI CTCAG 2 cut(s) 64, 156
BspTNI GGTCTC 1 cut(s) 146
BssECI CCNNGG 2 cut(s) 66, 424
BssT1I CCWWGG 2 cut(s) 66, 424
Bst4CI ACNGT 1 cut(s) 344
Bst6I CTCTTC 1 cut(s) 330
BstBAI YACGTR 1 cut(s) 232
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 2 cut(s) 51, 143
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 2 cut(s) 91, 190
BstHHI GCGC 1 cut(s) 237
BstMAI GTCTC 1 cut(s) 146
BstMWI GCNNNNNNNGC 2 cut(s) 62, 438
BstV1I GCAGC 1 cut(s) 292
Bsu36I CCTNAGG 1 cut(s) 51
BsuRI GGCC 1 cut(s) 371
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 2 cut(s) 91, 190
BtsIMutI CAGTG 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 39
CaiI CAGNNNCTG 1 cut(s) 82
CfoI GCGC 1 cut(s) 237
Csp6I GTAC 2 cut(s) 22, 47
CviAII CATG 2 cut(s) 25, 67
CviJI RGCY 9 cut(s) 10, 37, 56, 65, 274, 305, 371, 381, 441
CviKI_1 RGCY 9 cut(s) 10, 37, 56, 65, 274, 305, 371, 381, 441
CviQI GTAC 2 cut(s) 22, 47
DdeI CTNAG 2 cut(s) 51, 143
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco130I CCWWGG 2 cut(s) 66, 424
Eco31I GGTCTC 1 cut(s) 146
Eco72I CACGTG 1 cut(s) 232
Eco81I CCTNAGG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 42
EcoRI GAATTC 1 cut(s) 283
EcoT14I CCWWGG 2 cut(s) 66, 424
ErhI CCWWGG 2 cut(s) 66, 424
FaeI CATG 2 cut(s) 28, 70
FaiI YATR 8 cut(s) 26, 68, 150, 152, 362, 385, 398, 413
FaqI GGGAC 1 cut(s) 184
FatI CATG 2 cut(s) 24, 66
Fnu4HI GCNGC 1 cut(s) 306
FokI GGATG 2 cut(s) 98, 197
Fsp4HI GCNGC 1 cut(s) 306
FspBI CTAG 1 cut(s) 425
GlaI GCGC 1 cut(s) 236
GluI GCNGC 1 cut(s) 306
GsaI CCCAGC 1 cut(s) 171
HaeIII GGCC 1 cut(s) 371
HhaI GCGC 1 cut(s) 237
Hin1II CATG 2 cut(s) 28, 70
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HinfI GANTC 3 cut(s) 79, 268, 348
Hpy188I TCNGA 2 cut(s) 289, 376
Hpy188III TCNNGA 3 cut(s) 83, 212, 323
HpyAV CCTTC 1 cut(s) 284
HpyCH4III ACNGT 1 cut(s) 344
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 4, 89, 308
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 438
HpyF3I CTNAG 2 cut(s) 51, 143
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 2 cut(s) 28, 70
HspAI GCGC 1 cut(s) 235
LmnI GCTCC 1 cut(s) 264
LpnPI CCDG 6 cut(s) 38, 51, 68, 89, 130, 153
Lsp1109I GCAGC 1 cut(s) 292
LweI GCATC 1 cut(s) 76
MaeI CTAG 1 cut(s) 425
MaeII ACGT 1 cut(s) 231
MboII GAAGA 2 cut(s) 311, 347
MluCI AATT 3 cut(s) 283, 414, 445
MlyI GAGTC 1 cut(s) 73
MnlI CCTC 5 cut(s) 60, 152, 155, 273, 382
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 1 cut(s) 29
MspA1I CMGCKG 2 cut(s) 274, 305
MwoI GCNNNNNNNGC 2 cut(s) 62, 438
NcoI CCATGG 1 cut(s) 66
NlaIII CATG 2 cut(s) 28, 70
NmeAIII GCCGAG 1 cut(s) 218
PaeR7I CTCGAG 1 cut(s) 42
PfeI GAWTC 2 cut(s) 268, 348
PflFI GACNNNGTC 1 cut(s) 175
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 1 cut(s) 307
PleI GAGTC 1 cut(s) 73
PmaCI CACGTG 1 cut(s) 232
PmlI CACGTG 1 cut(s) 232
PpsI GAGTC 1 cut(s) 73
Ppu21I YACGTR 1 cut(s) 232
PspCI CACGTG 1 cut(s) 232
PspFI CCCAGC 1 cut(s) 167
PspXI VCTCGAGB 1 cut(s) 42
PstNI CAGNNNCTG 1 cut(s) 82
PsyI GACNNNGTC 1 cut(s) 175
PvuII CAGCTG 2 cut(s) 274, 305
RsaI GTAC 2 cut(s) 23, 48
RsaNI GTAC 2 cut(s) 22, 47
RseI CAYNNNNRTG 1 cut(s) 29
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 1 cut(s) 306
SchI GAGTC 1 cut(s) 73
SetI ASST 9 cut(s) 23, 52, 142, 149, 166, 234, 276, 307, 360
SfaNI GCATC 1 cut(s) 76
Sfr274I CTCGAG 1 cut(s) 42
SlaI CTCGAG 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 42
SmoI CTYRAG 1 cut(s) 42
Sse9I AATT 3 cut(s) 283, 414, 445
SspMI CTAG 1 cut(s) 425
StyI CCWWGG 2 cut(s) 66, 424
TaaI ACNGT 1 cut(s) 344
TaiI ACGT 1 cut(s) 234
TaqI TCGA 1 cut(s) 43
TasI AATT 3 cut(s) 283, 414, 445
TfiI GAWTC 2 cut(s) 268, 348
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 349
TseI GCWGC 1 cut(s) 305
TspDTI ATGAA 3 cut(s) 41, 47, 201
TspRI CASTG 1 cut(s) 349
Tth111I GACNNNGTC 1 cut(s) 175
Van91I CCANNNNNTGG 1 cut(s) 82
XapI RAATTY 1 cut(s) 283
XhoI CTCGAG 1 cut(s) 42
XmaJI CCTAGG 1 cut(s) 424
XspI CTAG 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.