RLG00000003116

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016350
Physical Location & Seq
Reverse (-)
44543270 .. 44545923
2654 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003116

Sequence Viewer

Length: 621 bp
ATGGCAGAGAAGACCGACCAGGCTTATCCTATTGCTCCTGCAAATGGTTACACAAGAAGCGATGGAGAGTCTTTGATTGTTGGTGGTGATTCTGTGGCTAACTGGATTTTGGGAGTTATCTGTGATCTAAAATTGCCGGAGTTACCCTCCATTGCTCCAATTCCGCTGCGAACGAGTTCTCCTATTATTGATAGCATCTCAAGTTCCTTTGGATGGAGGAGCATTTCTCCTGATGATCCAGTGAAATTGTCCCTGCTAGATGTACAATATGCGAAAAAAATGAAAACTGACAGCTGGCATATTCTCATTGGGATGAAAACCACTAAAAGTTCTACAGCTAATCAACAAATACCTCATTCTTTGCATCTATGTCTTCCAGTTCATGAAGCTCATGTCAAAATGCGATCAAAACATGCAAGCCTTCAAGGAAGTGGCCATCATAAGGCATCCACGTACTGGAGAATATGCATTTGGGTTCATTACTTCATCAGTTACCCTTCAGGATCCAAACTATTTATTCACTGGCTTCCTTCCATATTTGATGATTCAGCTAAATTAAAATTATGCTGGAGCTTAAAATTATCACAACCAAAGTTCGCAGCCAACCAAGCAAGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

207

Amino Acids

22.89

Weight (kDa)

9.26

Isoelectric Point (pI)

57.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 456
AciI CCGC 1 cut(s) 164
AclWI GGATC 3 cut(s) 230, 498, 511
AcoI YGGCCR 1 cut(s) 433
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 2 cut(s) 264, 455
AfiI CCNNNNNNNGG 4 cut(s) 44, 213, 442, 456
AgsI TTSAA 1 cut(s) 425
AjnI CCWGG 1 cut(s) 18
AjuI GAANNNNNNNTTGG 2 cut(s) 454, 486
AloI GAACNNNNNNTCC 2 cut(s) 163, 195
AluBI AGCT 5 cut(s) 294, 338, 389, 551, 573
AluI AGCT 5 cut(s) 294, 338, 389, 551, 573
AlwI GGATC 3 cut(s) 230, 498, 511
AoxI GGCC 1 cut(s) 433
ApeKI GCWGC 2 cut(s) 166, 599
Asp700I GAANNNNTTC 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 98
BalI TGGCCA 1 cut(s) 435
BamHI GGATCC 1 cut(s) 503
BbsI GAAGAC 2 cut(s) 17, 365
BbvI GCAGC 2 cut(s) 153, 611
BccI CCATC 3 cut(s) 56, 207, 444
BciT130I CCWGG 1 cut(s) 20
BfaI CTAG 1 cut(s) 257
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 2 cut(s) 167, 600
BlsI GCNGC 2 cut(s) 168, 601
Bme1390I CCNGG 1 cut(s) 20
BmiI GGNNCC 1 cut(s) 505
BmrFI CCNGG 1 cut(s) 20
BmsI GCATC 3 cut(s) 204, 373, 455
BpiI GAAGAC 2 cut(s) 17, 365
BplI GAGNNNNNCTC 4 cut(s) 131, 163, 211, 243
BpmI CTGGAG 2 cut(s) 478, 589
BpuEI CTTGAG 1 cut(s) 184
BsaAI YACGTR 1 cut(s) 453
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 213, 442, 456
Bse1I ACTGG 5 cut(s) 107, 239, 377, 461, 527
Bse3DI GCAATG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 20
BseGI GGATG 3 cut(s) 218, 318, 446
BseLI CCNNNNNNNGG 4 cut(s) 44, 213, 442, 456
BseMI GCAATG 1 cut(s) 150
BseNI ACTGG 5 cut(s) 107, 239, 377, 461, 527
BseRI GAGGAG 1 cut(s) 232
BseXI GCAGC 2 cut(s) 153, 611
BshFI GGCC 1 cut(s) 435
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 235
BslI CCNNNNNNNGG 4 cut(s) 44, 213, 442, 456
BsmFI GGGAC 1 cut(s) 235
BsnI GGCC 1 cut(s) 435
Bsp1407I TGTACA 1 cut(s) 262
Bsp143I GATC 4 cut(s) 124, 235, 404, 503
BspACI CCGC 1 cut(s) 164
BspANI GGCC 1 cut(s) 435
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 1 cut(s) 505
BspPI GGATC 3 cut(s) 230, 498, 511
BsrDI GCAATG 1 cut(s) 150
BsrGI TGTACA 1 cut(s) 262
BsrI ACTGG 5 cut(s) 107, 239, 377, 461, 527
BssMI GATC 4 cut(s) 124, 235, 404, 503
Bst2UI CCWGG 1 cut(s) 20
BstAUI TGTACA 1 cut(s) 262
BstBAI YACGTR 1 cut(s) 453
BstC8I GCNNGC 3 cut(s) 296, 418, 613
BstF5I GGATG 3 cut(s) 218, 318, 446
BstKTI GATC 4 cut(s) 127, 238, 407, 506
BstMBI GATC 4 cut(s) 124, 235, 404, 503
BstMWI GCNNNNNNNGC 1 cut(s) 608
BstNI CCWGG 1 cut(s) 20
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 18
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 153, 611
BstV2I GAAGAC 2 cut(s) 17, 365
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
BsuRI GGCC 1 cut(s) 435
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 3 cut(s) 218, 318, 446
BtsIMutI CAGTG 2 cut(s) 246, 520
Cac8I GCNNGC 3 cut(s) 296, 418, 613
CciI TCATGA 1 cut(s) 382
Csp6I GTAC 2 cut(s) 263, 454
CviAII CATG 3 cut(s) 383, 392, 413
CviQI GTAC 2 cut(s) 263, 454
DpnI GATC 4 cut(s) 126, 237, 406, 505
DpnII GATC 4 cut(s) 124, 235, 404, 503
EaeI YGGCCR 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 18
EcoT22I ATGCAT 1 cut(s) 470
FaeI CATG 3 cut(s) 386, 395, 416
FaqI GGGAC 1 cut(s) 235
FatI CATG 3 cut(s) 382, 391, 412
Fnu4HI GCNGC 2 cut(s) 167, 600
FokI GGATG 3 cut(s) 225, 325, 433
Fsp4HI GCNGC 2 cut(s) 167, 600
FspBI CTAG 1 cut(s) 257
GluI GCNGC 2 cut(s) 167, 600
GsuI CTGGAG 2 cut(s) 478, 589
HaeIII GGCC 1 cut(s) 435
HapII CCGG 1 cut(s) 137
Hin1II CATG 3 cut(s) 386, 395, 416
HinfI GANTC 3 cut(s) 68, 89, 545
HpaII CCGG 1 cut(s) 137
HphI GGTGA 1 cut(s) 98
Hpy188III TCNNGA 3 cut(s) 230, 383, 501
HpyAV CCTTC 3 cut(s) 431, 507, 540
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 4 cut(s) 41, 364, 416, 468
HpyF10VI GCNNNNNNNGC 1 cut(s) 608
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 3 cut(s) 386, 395, 416
Kzo9I GATC 4 cut(s) 124, 235, 404, 503
LmnI GCTCC 4 cut(s) 40, 160, 219, 570
Lsp1109I GCAGC 2 cut(s) 153, 611
LweI GCATC 3 cut(s) 204, 373, 455
MaeI CTAG 1 cut(s) 257
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 3 cut(s) 47, 141, 491
MalI GATC 4 cut(s) 126, 237, 406, 505
MboI GATC 4 cut(s) 124, 235, 404, 503
MboII GAAGA 2 cut(s) 22, 365
MflI RGATCY 1 cut(s) 503
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 6 cut(s) 131, 159, 245, 554, 560, 578
MluNI TGGCCA 1 cut(s) 435
MlyI GAGTC 1 cut(s) 77
MnlI CCTC 3 cut(s) 157, 210, 363
Mox20I TGGCCA 1 cut(s) 435
Mph1103I ATGCAT 1 cut(s) 470
MroXI GAANNNNTTC 1 cut(s) 175
MscI TGGCCA 1 cut(s) 435
MseI TTAA 2 cut(s) 557, 575
MslI CAYNNNNRTG 1 cut(s) 311
Msp20I TGGCCA 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 166, 294
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 1 cut(s) 20
MvaI CCWGG 1 cut(s) 20
MwoI GCNNNNNNNGC 1 cut(s) 608
NdeII GATC 4 cut(s) 124, 235, 404, 503
NlaIII CATG 3 cut(s) 386, 395, 416
NlaIV GGNNCC 1 cut(s) 505
NsiI ATGCAT 1 cut(s) 470
NspI RCATGY 1 cut(s) 416
PagI TCATGA 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 175
PfeI GAWTC 2 cut(s) 89, 545
PflMI CCANNNNNTGG 1 cut(s) 456
PkrI GCNGC 2 cut(s) 168, 601
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Ppu21I YACGTR 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 505
PsuI RGATCY 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 294
RsaI GTAC 2 cut(s) 264, 455
RsaNI GTAC 2 cut(s) 263, 454
RseI CAYNNNNRTG 1 cut(s) 311
SaqAI TTAA 2 cut(s) 557, 575
SatI GCNGC 2 cut(s) 167, 600
Sau3AI GATC 4 cut(s) 124, 235, 404, 503
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 1 cut(s) 20
SetI ASST 7 cut(s) 296, 340, 355, 391, 455, 553, 575
SfaNI GCATC 3 cut(s) 204, 373, 455
SfcI CTRYAG 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 6 cut(s) 131, 159, 245, 554, 560, 578
SsiI CCGC 1 cut(s) 164
SspMI CTAG 1 cut(s) 257
StyD4I CCNGG 1 cut(s) 18
TaiI ACGT 1 cut(s) 455
TaqII GACCGA 1 cut(s) 29
TasI AATT 6 cut(s) 131, 159, 245, 554, 560, 578
TatI WGTACW 1 cut(s) 262
TfiI GAWTC 2 cut(s) 89, 545
Tru1I TTAA 2 cut(s) 557, 575
Tru9I TTAA 2 cut(s) 557, 575
TscAI CASTG 2 cut(s) 246, 527
TseI GCWGC 2 cut(s) 166, 599
TspDTI ATGAA 6 cut(s) 296, 329, 371, 399, 467, 475
TspRI CASTG 2 cut(s) 246, 527
Van91I CCANNNNNTGG 1 cut(s) 456
XceI RCATGY 1 cut(s) 416
XmnI GAANNNNTTC 1 cut(s) 175
XspI CTAG 1 cut(s) 257
Zsp2I ATGCAT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.