RLG00000025328

Diacylglycerol kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016350
Physical Location & Seq
Reverse (-)
45033299 .. 45038087
4789 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025328

Sequence Viewer

Length: 570 bp
ATGGCACCTGGTTCCTCGAGCTACAGTGCATCTTCATCTGAAATTGTTGGTGGTGATTCTGTGGCTAACTGGATTTTGGGAGTTATCTGTGATCTAAAATTGCCGGAGTTACCCTCCATTGCTGCAATTCCGCTGCGAACGAGTTCTCCTATTATTGATAGCATCTCAAGTTCCTTTGGATGGTTGTACTTGAAATTGTCCCTGCTAGATGTACAATATGCAAAAAAAATGAAAACTGACAGCTGGCATATTCTCATTGGGATGAAAACCACTAAAAGTTCTACAGCTAATCAACAAATACCTCATTCTTTGCATCTATGTCTTCCAGTTCATGAAGCTCATGTCAAAATGCGATCAAAACATGCAAGCCTTCAAGGAAGTGGCCATCATAAGGCATCCACGTATCGGAGAATATGCATTTGGGTTCATTACTTCATCAGTTACCCTTCAGGATCCAAACTATTTATTCACTGGCTTCCTTCCATATTTGATGATTCAGCTAAATTAAAATTATGCTGGAGCTTAAAATTATCACAACCAAAGTTCGCAGCCAACCAAGCAAGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAGK_acc DAGK_cat NB-ARC

Protein Analysis

190

Amino Acids

20.92

Weight (kDa)

9.58

Isoelectric Point (pI)

61.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 1 cut(s) 131
AclWI GGATC 2 cut(s) 447, 460
AcoI YGGCCR 1 cut(s) 382
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 2 cut(s) 188, 213
AfiI CCNNNNNNNGG 3 cut(s) 180, 391, 405
AgsI TTSAA 2 cut(s) 193, 374
AjnI CCWGG 1 cut(s) 7
AjuI GAANNNNNNNTTGG 2 cut(s) 403, 435
AloI GAACNNNNNNTCC 2 cut(s) 130, 162
AluBI AGCT 6 cut(s) 21, 243, 287, 338, 500, 522
AluI AGCT 6 cut(s) 21, 243, 287, 338, 500, 522
AlwI GGATC 2 cut(s) 447, 460
Ama87I CYCGRG 1 cut(s) 16
AoxI GGCC 1 cut(s) 382
ApeKI GCWGC 3 cut(s) 122, 133, 548
Asp700I GAANNNNTTC 1 cut(s) 142
AsuHPI GGTGA 1 cut(s) 65
AvaI CYCGRG 1 cut(s) 16
BalI TGGCCA 1 cut(s) 384
BamHI GGATCC 1 cut(s) 452
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 314
BbvI GCAGC 3 cut(s) 109, 120, 560
BccI CCATC 2 cut(s) 174, 393
BciT130I CCWGG 1 cut(s) 9
BfaI CTAG 1 cut(s) 206
BfmI CTRYAG 2 cut(s) 22, 282
BisI GCNGC 3 cut(s) 123, 134, 549
BlsI GCNGC 3 cut(s) 124, 135, 550
Bme1390I CCNGG 1 cut(s) 9
BmeT110I CYCGRG 1 cut(s) 16
BmiI GGNNCC 3 cut(s) 6, 13, 454
BmrFI CCNGG 1 cut(s) 9
BmsI GCATC 4 cut(s) 38, 171, 322, 404
BpiI GAAGAC 1 cut(s) 314
BplI GAGNNNNNCTC 2 cut(s) 98, 130
BpmI CTGGAG 1 cut(s) 538
BpuEI CTTGAG 1 cut(s) 151
BsaAI YACGTR 1 cut(s) 402
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 391, 405
Bse1I ACTGG 3 cut(s) 74, 326, 476
Bse3DI GCAATG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 9
BseGI GGATG 3 cut(s) 185, 267, 395
BseLI CCNNNNNNNGG 3 cut(s) 180, 391, 405
BseMI GCAATG 1 cut(s) 117
BseNI ACTGG 3 cut(s) 74, 326, 476
BseXI GCAGC 3 cut(s) 109, 120, 560
BshFI GGCC 1 cut(s) 384
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 1 cut(s) 16
BsiSI CCGG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 3 cut(s) 180, 391, 405
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 384
BsoBI CYCGRG 1 cut(s) 16
Bsp1407I TGTACA 1 cut(s) 211
Bsp143I GATC 3 cut(s) 91, 353, 452
BspACI CCGC 1 cut(s) 131
BspANI GGCC 1 cut(s) 384
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 3 cut(s) 6, 13, 454
BspPI GGATC 2 cut(s) 447, 460
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 117
BsrGI TGTACA 1 cut(s) 211
BsrI ACTGG 3 cut(s) 74, 326, 476
BssMI GATC 3 cut(s) 91, 353, 452
Bst2UI CCWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 211
BstBAI YACGTR 1 cut(s) 402
BstC8I GCNNGC 3 cut(s) 245, 367, 562
BstF5I GGATG 3 cut(s) 185, 267, 395
BstKTI GATC 3 cut(s) 94, 356, 455
BstMBI GATC 3 cut(s) 91, 353, 452
BstMWI GCNNNNNNNGC 1 cut(s) 557
BstNI CCWGG 1 cut(s) 9
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 7
BstSFI CTRYAG 2 cut(s) 22, 282
BstV1I GCAGC 3 cut(s) 109, 120, 560
BstV2I GAAGAC 1 cut(s) 314
BstX2I RGATCY 1 cut(s) 452
BstYI RGATCY 1 cut(s) 452
BsuRI GGCC 1 cut(s) 384
BtsCI GGATG 3 cut(s) 185, 267, 395
BtsIMutI CAGTG 2 cut(s) 31, 469
Cac8I GCNNGC 3 cut(s) 245, 367, 562
CciI TCATGA 1 cut(s) 331
CsiI ACCWGGT 1 cut(s) 7
Csp6I GTAC 2 cut(s) 187, 212
CviAII CATG 3 cut(s) 332, 341, 362
CviQI GTAC 2 cut(s) 187, 212
DpnI GATC 3 cut(s) 93, 355, 454
DpnII GATC 3 cut(s) 91, 353, 452
EaeI YGGCCR 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 432
Eco88I CYCGRG 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 7
EcoT22I ATGCAT 1 cut(s) 419
FaeI CATG 3 cut(s) 335, 344, 365
FaqI GGGAC 1 cut(s) 184
FatI CATG 3 cut(s) 331, 340, 361
Fnu4HI GCNGC 3 cut(s) 123, 134, 549
FokI GGATG 3 cut(s) 192, 274, 382
Fsp4HI GCNGC 3 cut(s) 123, 134, 549
FspBI CTAG 1 cut(s) 206
GluI GCNGC 3 cut(s) 123, 134, 549
GsuI CTGGAG 1 cut(s) 538
HaeIII GGCC 1 cut(s) 384
HapII CCGG 1 cut(s) 104
Hin1II CATG 3 cut(s) 335, 344, 365
HinfI GANTC 2 cut(s) 56, 494
HpaII CCGG 1 cut(s) 104
HphI GGTGA 1 cut(s) 65
Hpy188I TCNGA 2 cut(s) 40, 408
Hpy188III TCNNGA 2 cut(s) 332, 450
HpyAV CCTTC 3 cut(s) 380, 456, 489
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 6 cut(s) 29, 125, 221, 313, 365, 417
HpyF10VI GCNNNNNNNGC 1 cut(s) 557
HpySE526I ACGT 1 cut(s) 401
Hsp92II CATG 3 cut(s) 335, 344, 365
Kzo9I GATC 3 cut(s) 91, 353, 452
LmnI GCTCC 1 cut(s) 519
LpnPI CCDG 9 cut(s) 21, 55, 117, 215, 229, 339, 435, 457, 502
Lsp1109I GCAGC 3 cut(s) 109, 120, 560
LweI GCATC 4 cut(s) 38, 171, 322, 404
MabI ACCWGGT 1 cut(s) 7
MaeI CTAG 1 cut(s) 206
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 2 cut(s) 108, 440
MalI GATC 3 cut(s) 93, 355, 454
MboI GATC 3 cut(s) 91, 353, 452
MboII GAAGA 2 cut(s) 24, 314
MflI RGATCY 1 cut(s) 452
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 7 cut(s) 42, 98, 126, 194, 503, 509, 527
MluNI TGGCCA 1 cut(s) 384
MnlI CCTC 3 cut(s) 25, 124, 312
Mox20I TGGCCA 1 cut(s) 384
Mph1103I ATGCAT 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 142
MscI TGGCCA 1 cut(s) 384
MseI TTAA 2 cut(s) 506, 524
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 384
MspA1I CMGCKG 2 cut(s) 133, 243
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 1 cut(s) 9
MvaI CCWGG 1 cut(s) 9
MwoI GCNNNNNNNGC 1 cut(s) 557
NdeII GATC 3 cut(s) 91, 353, 452
NlaIII CATG 3 cut(s) 335, 344, 365
NlaIV GGNNCC 3 cut(s) 6, 13, 454
NsiI ATGCAT 1 cut(s) 419
NspI RCATGY 1 cut(s) 365
PaeR7I CTCGAG 1 cut(s) 16
PagI TCATGA 1 cut(s) 331
PdmI GAANNNNTTC 1 cut(s) 142
PfeI GAWTC 2 cut(s) 56, 494
PkrI GCNGC 3 cut(s) 124, 135, 550
Ppu21I YACGTR 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 7
PspGI CCWGG 1 cut(s) 7
PspN4I GGNNCC 3 cut(s) 6, 13, 454
PspXI VCTCGAGB 1 cut(s) 16
PsuI RGATCY 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 243
RsaI GTAC 2 cut(s) 188, 213
RsaNI GTAC 2 cut(s) 187, 212
RseI CAYNNNNRTG 1 cut(s) 260
SaqAI TTAA 2 cut(s) 506, 524
SatI GCNGC 3 cut(s) 123, 134, 549
Sau3AI GATC 3 cut(s) 91, 353, 452
ScrFI CCNGG 1 cut(s) 9
SetI ASST 9 cut(s) 10, 23, 245, 289, 304, 340, 404, 502, 524
SexAI ACCWGGT 1 cut(s) 7
SfaNI GCATC 4 cut(s) 38, 171, 322, 404
SfcI CTRYAG 2 cut(s) 22, 282
Sfr274I CTCGAG 1 cut(s) 16
SlaI CTCGAG 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 2 cut(s) 16, 166
SmoI CTYRAG 2 cut(s) 16, 166
Sse9I AATT 7 cut(s) 42, 98, 126, 194, 503, 509, 527
SsiI CCGC 1 cut(s) 131
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 1 cut(s) 7
TaaI ACNGT 1 cut(s) 26
TaiI ACGT 1 cut(s) 404
TaqI TCGA 1 cut(s) 17
TasI AATT 7 cut(s) 42, 98, 126, 194, 503, 509, 527
TatI WGTACW 2 cut(s) 186, 211
TfiI GAWTC 2 cut(s) 56, 494
Tru1I TTAA 2 cut(s) 506, 524
Tru9I TTAA 2 cut(s) 506, 524
TscAI CASTG 2 cut(s) 31, 476
TseI GCWGC 3 cut(s) 122, 133, 548
TspDTI ATGAA 7 cut(s) 24, 245, 278, 320, 348, 416, 424
TspRI CASTG 2 cut(s) 31, 476
XceI RCATGY 1 cut(s) 365
XhoI CTCGAG 1 cut(s) 16
XmnI GAANNNNTTC 1 cut(s) 142
XspI CTAG 1 cut(s) 206
Zsp2I ATGCAT 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.