Rh5BG321700

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016350
Physical Location & Seq
Forward (+)
43116806 .. 43124175
7370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG321700.1

Sequence Viewer

Length: 1653 bp
ATGGAGAGCTTGATTCTGGTACCAGTGCATCTCTTGCCCAACAGGATCTCTCGGTTAACCTTATTAGTTTCTTTGTTGTTCTTAGCAATTCCATTAGAAGCAGCCAACACCTACCCAAAAAAATGTGGAAATCTCAACCTCACATTGCCCTTTGAATTTGGCAGTCCCGTAAAAGGAAGTGGATCTTCTATAGGTGATTACTGGTTTGACATAAGACATAGCAGCACTTCCTATAGAACACCAACATCCTTCATAGGCGAAACTAGTCTTGAGGTACAGGTACAGGTTTCTTCATTTAGCAAGGTACGCGTAAATAACTCCATTGTTTACGCTTATAGTAATTATAGCTGCTACACTGAAGCTGGAGCGAAGATGTTGCACGCAAGCTATCTCCATGCTGCTGCAATCAATTTGACAGATGGCGCCCAGATTATTAGAACCTCCAACACTATTACTGCAAACAAGTTCACATTGATAGGTTATGAGTCATTTCATTTTGTAGGTTATGACCCATTAATTTGCAGCATCAACAGTACAATGATTAATCAGGAAAGAAGAGCTTGTTCTGGTATTAACTGTGGCCATTCTTTCATTCGAAGAGGCATCAAGTCCTTTCTGAAAGAGGCAGAAAGTGTAGGAAACATTACTTTGTTACCTAAAGGTTGGTTCATGAGAACGAGTAGCAAGCCGCTCAGAACTGGGGTTCCTGTGTCTATGGTAGAACACATCAGCTATGCGAGTCGTCGCACTCTACGGCTACCAGGCAGCTCCAGGTTGAAATTATCAGCTCAACACTCTCTATGGTATTCCAATTCAAAAAAGGGATTCCTTGTATCAAAGGTTCGCGAAGTTGACACACATAAAAATCTGGAAAACCACATGAAAAAGGACAGAGGTTTCATTGCACACATCAAAGTCTTTTGGGAAAATTTCACTCTAGGTCTTGGCTTTTTTTGCTTGTTTCTTGTGTTTGGGATCACTGGGATATATTCCAGTGTGAAGAAACAAAAGCCAAGGCTTGTTGGGAGGTTAGCGAAGCAGTCTGCTAAGCTGCATCCTTCTCTGCTGTCTGGTGGTGGTCTTTTAAAAACTGCTCCAATCTGTGAAATTGTTGGTGGTGATTCTGTGGCTAACTGGATTTTGGGAGTTATCTGTGATCTAAAATTGCCGGAGTTACCCTCCATTGCTCCAATTCCGCTGCAAACGAGTTCTCCTATTATTGATAGCATCTCAAGTTCCTTTGGATGGAGGAGCATTTCTCCTGATGATCCAGTGAAATTGTCCCTGCTAGATGTACAATATGCGAAAAAAATGAAAACTGACAGCTGGCATATTCTCATTGGGATGAAAACCACTAAAAGTTCTACAGCTAATCAACAAATACCTCATTCTTTGCATCTATGTCTTCCAGTTCATGAAGCTCATGTCAAAATGCGATCAAAACATGCAAGCCTTCAAGGAAGTGGCCATCATAAGGCATCCACGTATTGGAGAATATGCATTTGGGTTCATTACTTCATCAGTTACCCTTCAGGATCCAAACTATTTATTCACTGGCTTCCTTCCATGTTTGATGATTCAGCTAAATTAAAATTATGCTGGAGCTTAAAATTATCACAACCAAAGTTCGCAGCCAACCAAGCAAGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

550

Amino Acids

61.19

Weight (kDa)

9.76

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 19
AccB1I GGYRCC 2 cut(s) 19, 422
AccB7I CCANNNNNTGG 1 cut(s) 1488
AccBSI CCGCTC 1 cut(s) 691
AccII CGCG 2 cut(s) 309, 846
AciI CCGC 2 cut(s) 689, 1196
AclWI GGATC 6 cut(s) 53, 190, 983, 1262, 1530, 1543
AcoI YGGCCR 2 cut(s) 580, 1465
AcsI RAATTY 2 cut(s) 155, 928
AcuI CTGAAG 2 cut(s) 378, 1515
AcyI GRCGYC 1 cut(s) 423
AfaI GTAC 6 cut(s) 21, 276, 282, 306, 535, 1296
AfiI CCNNNNNNNGG 4 cut(s) 173, 1245, 1474, 1488
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 4 cut(s) 155, 778, 816, 1457
AhlI ACTAGT 1 cut(s) 263
AjnI CCWGG 2 cut(s) 760, 770
AjuI GAANNNNNNNTTGG 2 cut(s) 1486, 1518
AloI GAACNNNNNNTCC 2 cut(s) 688, 720
AlwI GGATC 6 cut(s) 53, 190, 983, 1262, 1530, 1543
AoxI GGCC 2 cut(s) 580, 1465
ApoI RAATTY 2 cut(s) 155, 928
AseI ATTAAT 2 cut(s) 515, 543
Asp718I GGTACC 1 cut(s) 19
AspLEI GCGC 1 cut(s) 425
AsuHPI GGTGA 2 cut(s) 206, 1130
AsuII TTCGAA 1 cut(s) 595
BalI TGGCCA 2 cut(s) 582, 1467
BamHI GGATCC 1 cut(s) 1535
BanI GGYRCC 2 cut(s) 19, 422
BbsI GAAGAC 1 cut(s) 1397
BccI CCATC 3 cut(s) 413, 1239, 1476
BceAI ACGGC 1 cut(s) 770
BcgI CGANNNNNNTGC 2 cut(s) 358, 392
BciT130I CCWGG 2 cut(s) 762, 772
BcuI ACTAGT 1 cut(s) 263
BfaI CTAG 3 cut(s) 264, 938, 1289
BfmI CTRYAG 3 cut(s) 189, 232, 1365
BfoI RGCGCY 1 cut(s) 426
BlpI GCTNAGC 1 cut(s) 1047
Bme1390I CCNGG 2 cut(s) 762, 772
BmiI GGNNCC 4 cut(s) 21, 424, 705, 1537
BmrFI CCNGG 2 cut(s) 762, 772
BmrI ACTGGG 2 cut(s) 708, 990
BmsI GCATC 7 cut(s) 37, 534, 612, 1063, 1236, 1405, 1487
BmuI ACTGGG 2 cut(s) 708, 990
BpiI GAAGAC 1 cut(s) 1397
BplI GAGNNNNNCTC 4 cut(s) 1163, 1195, 1243, 1275
BpmI CTGGAG 3 cut(s) 384, 754, 1621
Bpu1102I GCTNAGC 1 cut(s) 1047
Bpu14I TTCGAA 1 cut(s) 595
BpuEI CTTGAG 2 cut(s) 290, 1216
BsaAI YACGTR 1 cut(s) 1485
BsaHI GRCGYC 1 cut(s) 423
BsaJI CCNNGG 1 cut(s) 1013
BsaXI ACNNNNNCTCC 2 cut(s) 1195, 1225
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 1245, 1474, 1488
Bse1I ACTGG 9 cut(s) 23, 206, 703, 985, 993, 1139, 1271, 1409, 1559
Bse3DI GCAATG 3 cut(s) 143, 900, 1182
BseBI CCWGG 2 cut(s) 762, 772
BseDI CCNNGG 1 cut(s) 1013
BseGI GGATG 5 cut(s) 245, 1054, 1250, 1350, 1478
BseLI CCNNNNNNNGG 4 cut(s) 173, 1245, 1474, 1488
BseMI GCAATG 3 cut(s) 143, 900, 1182
BseMII CTCAG 1 cut(s) 706
BseNI ACTGG 9 cut(s) 23, 206, 703, 985, 993, 1139, 1271, 1409, 1559
BseRI GAGGAG 1 cut(s) 1264
Bsh1236I CGCG 2 cut(s) 309, 846
BshFI GGCC 2 cut(s) 582, 1467
BshNI GGYRCC 2 cut(s) 19, 422
BsiSI CCGG 1 cut(s) 1169
BslFI GGGAC 2 cut(s) 150, 1267
BslI CCNNNNNNNGG 4 cut(s) 173, 1245, 1474, 1488
BsmFI GGGAC 2 cut(s) 150, 1267
BsnI GGCC 2 cut(s) 582, 1467
Bsp119I TTCGAA 1 cut(s) 595
Bsp1407I TGTACA 1 cut(s) 1294
Bsp143I GATC 7 cut(s) 45, 182, 975, 1156, 1267, 1436, 1535
Bsp1720I GCTNAGC 1 cut(s) 1047
Bsp68I TCGCGA 1 cut(s) 846
BspACI CCGC 2 cut(s) 689, 1196
BspANI GGCC 2 cut(s) 582, 1467
BspCNI CTCAG 1 cut(s) 705
BspFNI CGCG 2 cut(s) 309, 846
BspHI TCATGA 2 cut(s) 669, 1414
BspLI GGNNCC 4 cut(s) 21, 424, 705, 1537
BspPI GGATC 6 cut(s) 53, 190, 983, 1262, 1530, 1543
BspQI GCTCTTC 1 cut(s) 550
BspT104I TTCGAA 1 cut(s) 595
BspT107I GGYRCC 2 cut(s) 19, 422
BsrBI CCGCTC 1 cut(s) 691
BsrDI GCAATG 3 cut(s) 143, 900, 1182
BsrGI TGTACA 1 cut(s) 1294
BsrI ACTGG 9 cut(s) 23, 206, 703, 985, 993, 1139, 1271, 1409, 1559
BssECI CCNNGG 1 cut(s) 1013
BssMI GATC 7 cut(s) 45, 182, 975, 1156, 1267, 1436, 1535
BssNI GRCGYC 1 cut(s) 423
BssT1I CCWWGG 1 cut(s) 1013
Bst2UI CCWGG 2 cut(s) 762, 772
Bst4CI ACNGT 2 cut(s) 533, 578
Bst6I CTCTTC 2 cut(s) 550, 592
BstACI GRCGYC 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 34
BstAUI TGTACA 1 cut(s) 1294
BstBAI YACGTR 1 cut(s) 1485
BstBI TTCGAA 1 cut(s) 595
BstC8I GCNNGC 6 cut(s) 381, 385, 686, 1328, 1450, 1645
BstDEI CTNAG 3 cut(s) 82, 692, 1047
BstF5I GGATG 5 cut(s) 245, 1054, 1250, 1350, 1478
BstFNI CGCG 2 cut(s) 309, 846
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 7 cut(s) 48, 185, 978, 1159, 1270, 1439, 1538
BstMBI GATC 7 cut(s) 45, 182, 975, 1156, 1267, 1436, 1535
BstMWI GCNNNNNNNGC 4 cut(s) 34, 306, 954, 1640
BstNI CCWGG 2 cut(s) 762, 772
BstNSI RCATGY 1 cut(s) 1448
BstSCI CCNGG 2 cut(s) 760, 770
BstSFI CTRYAG 3 cut(s) 189, 232, 1365
BstUI CGCG 2 cut(s) 309, 846
BstV2I GAAGAC 1 cut(s) 1397
BstX2I RGATCY 3 cut(s) 45, 182, 1535
BstYI RGATCY 3 cut(s) 45, 182, 1535
BsuRI GGCC 2 cut(s) 582, 1467
BtsCI GGATG 5 cut(s) 245, 1054, 1250, 1350, 1478
BtsIMutI CAGTG 6 cut(s) 30, 354, 978, 1000, 1278, 1552
BtuMI TCGCGA 1 cut(s) 846
Cac8I GCNNGC 6 cut(s) 381, 385, 686, 1328, 1450, 1645
CciI TCATGA 2 cut(s) 669, 1414
CfoI GCGC 1 cut(s) 425
Csp6I GTAC 6 cut(s) 20, 275, 281, 305, 534, 1295
CviAII CATG 7 cut(s) 395, 670, 880, 1415, 1424, 1445, 1567
CviQI GTAC 6 cut(s) 20, 275, 281, 305, 534, 1295
DdeI CTNAG 3 cut(s) 82, 692, 1047
DinI GGCGCC 1 cut(s) 424
DpnI GATC 7 cut(s) 47, 184, 977, 1158, 1269, 1438, 1537
DpnII GATC 7 cut(s) 45, 182, 975, 1156, 1267, 1436, 1535
DraI TTTAAA 1 cut(s) 1086
EaeI YGGCCR 2 cut(s) 580, 1465
Eam1104I CTCTTC 2 cut(s) 550, 592
EarI CTCTTC 2 cut(s) 550, 592
Eco130I CCWWGG 1 cut(s) 1013
Eco57I CTGAAG 2 cut(s) 378, 1515
EcoRII CCWGG 2 cut(s) 760, 770
EcoT14I CCWWGG 1 cut(s) 1013
EcoT22I ATGCAT 1 cut(s) 1502
EgeI GGCGCC 1 cut(s) 424
EheI GGCGCC 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 1013
FaeI CATG 7 cut(s) 398, 673, 883, 1418, 1427, 1448, 1570
FalI AAGNNNNNCTT 4 cut(s) 169, 201, 544, 576
FaqI GGGAC 2 cut(s) 150, 1267
FatI CATG 7 cut(s) 394, 669, 879, 1414, 1423, 1444, 1566
FokI GGATG 5 cut(s) 232, 1041, 1257, 1357, 1465
FspBI CTAG 3 cut(s) 264, 938, 1289
GlaI GCGC 1 cut(s) 424
GsuI CTGGAG 3 cut(s) 384, 754, 1621
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 2 cut(s) 582, 1467
HapII CCGG 1 cut(s) 1169
HhaI GCGC 1 cut(s) 425
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 7 cut(s) 398, 673, 883, 1418, 1427, 1448, 1570
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HincII GTYRAC 2 cut(s) 57, 853
HindII GTYRAC 2 cut(s) 57, 853
HinfI GANTC 6 cut(s) 13, 485, 739, 825, 1121, 1577
HpaI GTTAAC 1 cut(s) 57
HpaII CCGG 1 cut(s) 1169
HphI GGTGA 2 cut(s) 206, 1130
Hpy166II GTNNAC 4 cut(s) 57, 328, 468, 853
Hpy188I TCNGA 2 cut(s) 618, 695
Hpy188III TCNNGA 8 cut(s) 269, 548, 670, 845, 869, 1262, 1415, 1533
Hpy8I GTNNAC 4 cut(s) 57, 328, 468, 853
Hpy99I CGWCG 1 cut(s) 747
HpyAV CCTTC 5 cut(s) 259, 1068, 1463, 1539, 1572
HpyCH4III ACNGT 2 cut(s) 533, 578
HpyCH4IV ACGT 1 cut(s) 1484
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 306, 954, 1640
HpyF3I CTNAG 3 cut(s) 82, 692, 1047
HpySE526I ACGT 1 cut(s) 1484
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 7 cut(s) 398, 673, 883, 1418, 1427, 1448, 1570
HspAI GCGC 1 cut(s) 423
KasI GGCGCC 1 cut(s) 422
KpnI GGTACC 1 cut(s) 23
KspAI GTTAAC 1 cut(s) 57
Kzo9I GATC 7 cut(s) 45, 182, 975, 1156, 1267, 1436, 1535
LguI GCTCTTC 1 cut(s) 550
LmnI GCTCC 6 cut(s) 365, 773, 1099, 1192, 1251, 1602
LweI GCATC 7 cut(s) 37, 534, 612, 1063, 1236, 1405, 1487
MaeI CTAG 3 cut(s) 264, 938, 1289
MaeII ACGT 1 cut(s) 1484
MaeIII GTNAC 3 cut(s) 651, 1173, 1523
MalI GATC 7 cut(s) 47, 184, 977, 1158, 1269, 1438, 1537
MbiI CCGCTC 1 cut(s) 691
MboI GATC 7 cut(s) 45, 182, 975, 1156, 1267, 1436, 1535
MboII GAAGA 7 cut(s) 177, 282, 382, 567, 609, 1012, 1397
MflI RGATCY 3 cut(s) 45, 182, 1535
MlsI TGGCCA 2 cut(s) 582, 1467
MluI ACGCGT 1 cut(s) 307
MluNI TGGCCA 2 cut(s) 582, 1467
Mly113I GGCGCC 1 cut(s) 423
MlyI GAGTC 2 cut(s) 494, 748
MmeI TCCRAC 1 cut(s) 468
Mox20I TGGCCA 2 cut(s) 582, 1467
Mph1103I ATGCAT 1 cut(s) 1502
MscI TGGCCA 2 cut(s) 582, 1467
MseI TTAA 7 cut(s) 56, 515, 543, 573, 1085, 1589, 1607
MslI CAYNNNNRTG 1 cut(s) 1343
Msp20I TGGCCA 2 cut(s) 582, 1467
MspA1I CMGCKG 2 cut(s) 1198, 1326
MspI CCGG 1 cut(s) 1169
MspR9I CCNGG 2 cut(s) 762, 772
MvaI CCWGG 2 cut(s) 762, 772
MvnI CGCG 2 cut(s) 309, 846
MwoI GCNNNNNNNGC 4 cut(s) 34, 306, 954, 1640
NarI GGCGCC 1 cut(s) 423
NdeII GATC 7 cut(s) 45, 182, 975, 1156, 1267, 1436, 1535
NlaIII CATG 7 cut(s) 398, 673, 883, 1418, 1427, 1448, 1570
NlaIV GGNNCC 4 cut(s) 21, 424, 705, 1537
NruI TCGCGA 1 cut(s) 846
NsiI ATGCAT 1 cut(s) 1502
NspI RCATGY 1 cut(s) 1448
NspV TTCGAA 1 cut(s) 595
PagI TCATGA 2 cut(s) 669, 1414
PciSI GCTCTTC 1 cut(s) 550
PfeI GAWTC 4 cut(s) 13, 825, 1121, 1577
PflMI CCANNNNNTGG 1 cut(s) 1488
PleI GAGTC 2 cut(s) 493, 747
PluTI GGCGCC 1 cut(s) 426
PpsI GAGTC 2 cut(s) 493, 747
Ppu21I YACGTR 1 cut(s) 1485
PshBI ATTAAT 2 cut(s) 515, 543
Psp6I CCWGG 2 cut(s) 760, 770
PspGI CCWGG 2 cut(s) 760, 770
PspN4I GGNNCC 4 cut(s) 21, 424, 705, 1537
PsrI GAACNNNNNNTAC 2 cut(s) 825, 857
PsuI RGATCY 3 cut(s) 45, 182, 1535
PvuII CAGCTG 1 cut(s) 1326
RruI TCGCGA 1 cut(s) 846
RsaI GTAC 6 cut(s) 21, 276, 282, 306, 535, 1296
RsaNI GTAC 6 cut(s) 20, 275, 281, 305, 534, 1295
RseI CAYNNNNRTG 1 cut(s) 1343
SapI GCTCTTC 1 cut(s) 550
SaqAI TTAA 7 cut(s) 56, 515, 543, 573, 1085, 1589, 1607
Sau3AI GATC 7 cut(s) 45, 182, 975, 1156, 1267, 1436, 1535
SchI GAGTC 2 cut(s) 494, 748
ScrFI CCNGG 2 cut(s) 762, 772
SfaNI GCATC 7 cut(s) 37, 534, 612, 1063, 1236, 1405, 1487
SfcI CTRYAG 3 cut(s) 189, 232, 1365
SfoI GGCGCC 1 cut(s) 424
SfuI TTCGAA 1 cut(s) 595
SmiMI CAYNNNNRTG 1 cut(s) 1343
SmlI CTYRAG 2 cut(s) 269, 1231
SmoI CTYRAG 2 cut(s) 269, 1231
SpeI ACTAGT 1 cut(s) 263
SsiI CCGC 2 cut(s) 689, 1196
SspDI GGCGCC 1 cut(s) 422
SspMI CTAG 3 cut(s) 264, 938, 1289
StyD4I CCNGG 2 cut(s) 760, 770
StyI CCWWGG 1 cut(s) 1013
TaaI ACNGT 2 cut(s) 533, 578
TaiI ACGT 1 cut(s) 1487
TaqI TCGA 1 cut(s) 595
TatI WGTACW 2 cut(s) 533, 1294
TauI GCSGC 1 cut(s) 691
TfiI GAWTC 4 cut(s) 13, 825, 1121, 1577
Tru1I TTAA 7 cut(s) 56, 515, 543, 573, 1085, 1589, 1607
Tru9I TTAA 7 cut(s) 56, 515, 543, 573, 1085, 1589, 1607
TscAI CASTG 6 cut(s) 30, 361, 985, 1000, 1278, 1559
TspRI CASTG 6 cut(s) 30, 361, 985, 1000, 1278, 1559
Van91I CCANNNNNTGG 1 cut(s) 1488
VspI ATTAAT 2 cut(s) 515, 543
XapI RAATTY 2 cut(s) 155, 928
XceI RCATGY 1 cut(s) 1448
XspI CTAG 3 cut(s) 264, 938, 1289
Zsp2I ATGCAT 1 cut(s) 1502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.