RLG00000003472

SAD/SRA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015631
Physical Location & Seq
Forward (+)
48852244 .. 48852812
569 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003472

Sequence Viewer

Length: 339 bp
ATGAGTGGCATGGGAAAAATTGATCCAGGAGGACTTGCTCCCATGCAAGGCAAACAAATCATGGGAGATGTCCCGGGTGTTGAAGTTGGTGTAAAGTTCCGTTATGAGTTGAGCTCGATCACTATTGCTGGCTTACATCGTCAGACAGAAGGTGGAATAGACTATGCATATTTTTGTAGGGAATCACTTACAACCAGTACTGTAGCATCTGGAGGATATGCTAACTATTTCCATGACTTGAACTCCTTGACTTACACCGGCCATGCATGGAGGAAGTGTGACGCATGCATCATAGGCTCCCAGAACATCAAGAGCGTAAACGTGGAAACCTTGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
SAD_SRA

Protein Analysis

113

Amino Acids

12.07

Weight (kDa)

6.25

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 21 - 91 3.5e-11 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 17
AcoI YGGCCR 1 cut(s) 259
AfaI GTAC 1 cut(s) 199
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 2 cut(s) 83, 241
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 1 cut(s) 114
AluI AGCT 1 cut(s) 114
Alw21I GWGCWC 1 cut(s) 116
AlwI GGATC 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 73
AoxI GGCC 1 cut(s) 259
AsuC2I CCSGG 2 cut(s) 74, 75
AvaI CYCGRG 1 cut(s) 73
BanII GRGCYC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 27
BcnI CCSGG 2 cut(s) 74, 75
BfmI CTRYAG 1 cut(s) 201
BmcAI AGTACT 1 cut(s) 199
Bme1390I CCNGG 3 cut(s) 27, 74, 75
BmeT110I CYCGRG 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 3 cut(s) 27, 74, 75
BmsI GCATC 2 cut(s) 215, 297
BplI GAGNNNNNCTC 2 cut(s) 98, 130
BpmI CTGGAG 1 cut(s) 231
BpuMI CCSGG 2 cut(s) 74, 75
BsaJI CCNNGG 1 cut(s) 73
BsaXI ACNNNNNCTCC 4 cut(s) 227, 257, 262, 292
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 195
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 73
BseLI CCNNNNNNNGG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 195
BshFI GGCC 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 116
BsiHKCI CYCGRG 1 cut(s) 73
BsiSI CCGG 2 cut(s) 74, 258
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 1 cut(s) 261
BsoBI CYCGRG 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 116
Bsp143I GATC 2 cut(s) 22, 117
BspANI GGCC 1 cut(s) 261
BspLI GGNNCC 1 cut(s) 298
BspPI GGATC 1 cut(s) 17
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 1 cut(s) 195
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 2 cut(s) 22, 117
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 1 cut(s) 202
BstC8I GCNNGC 2 cut(s) 130, 286
BstKTI GATC 2 cut(s) 25, 120
BstMBI GATC 2 cut(s) 22, 117
BstMWI GCNNNNNNNGC 1 cut(s) 294
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 288
BstSCI CCNGG 3 cut(s) 25, 72, 73
BstSFI CTRYAG 1 cut(s) 201
BsuRI GGCC 1 cut(s) 261
Cac8I GCNNGC 2 cut(s) 130, 286
Cfr10I RCCGGY 1 cut(s) 257
Cfr9I CCCGGG 1 cut(s) 73
CseI GACGC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 198
CviAII CATG 7 cut(s) 10, 43, 61, 233, 263, 267, 285
CviJI RGCY 4 cut(s) 114, 132, 261, 297
CviKI_1 RGCY 4 cut(s) 114, 132, 261, 297
CviQI GTAC 1 cut(s) 198
DpnI GATC 2 cut(s) 24, 119
DpnII GATC 2 cut(s) 22, 117
EaeI YGGCCR 1 cut(s) 259
Ecl136II GAGCTC 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 116
Eco53kI GAGCTC 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 25
EcoT22I ATGCAT 3 cut(s) 169, 268, 290
EcoT38I GRGCYC 1 cut(s) 116
FaeI CATG 7 cut(s) 13, 46, 64, 236, 266, 270, 288
FaqI GGGAC 1 cut(s) 56
FatI CATG 7 cut(s) 9, 42, 60, 232, 262, 266, 284
FriOI GRGCYC 1 cut(s) 116
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 1 cut(s) 261
HapII CCGG 2 cut(s) 74, 258
HgaI GACGC 1 cut(s) 290
Hin1II CATG 7 cut(s) 13, 46, 64, 236, 266, 270, 288
HinfI GANTC 1 cut(s) 182
HpaII CCGG 2 cut(s) 74, 258
Hpy166II GTNNAC 1 cut(s) 319
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 2 cut(s) 210, 310
Hpy8I GTNNAC 1 cut(s) 319
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 4 cut(s) 46, 167, 266, 288
HpyF10VI GCNNNNNNNGC 1 cut(s) 294
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 7 cut(s) 13, 46, 64, 236, 266, 270, 288
Kzo9I GATC 2 cut(s) 22, 117
LmnI GCTCC 2 cut(s) 43, 302
LpnPI CCDG 8 cut(s) 12, 39, 87, 114, 195, 208, 271, 314
LweI GCATC 2 cut(s) 215, 297
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 278
MalI GATC 2 cut(s) 24, 119
MboI GATC 2 cut(s) 22, 117
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 1 cut(s) 18
MnlI CCTC 3 cut(s) 23, 206, 264
Mph1103I ATGCAT 3 cut(s) 169, 268, 290
MspI CCGG 2 cut(s) 74, 258
MspR9I CCNGG 3 cut(s) 27, 74, 75
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 294
NciI CCSGG 2 cut(s) 74, 75
NdeII GATC 2 cut(s) 22, 117
NlaIII CATG 7 cut(s) 13, 46, 64, 236, 266, 270, 288
NlaIV GGNNCC 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 3 cut(s) 169, 268, 290
NspI RCATGY 1 cut(s) 288
PaeI GCATGC 1 cut(s) 288
PfeI GAWTC 1 cut(s) 182
PfoI TCCNGGA 1 cut(s) 25
Psp124BI GAGCTC 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 298
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
SacI GAGCTC 1 cut(s) 116
Sau3AI GATC 2 cut(s) 22, 117
ScaI AGTACT 1 cut(s) 199
ScrFI CCNGG 3 cut(s) 27, 74, 75
SduI GDGCHC 1 cut(s) 116
SetI ASST 4 cut(s) 116, 154, 324, 332
SfaNI GCATC 2 cut(s) 215, 297
SfcI CTRYAG 1 cut(s) 201
SmaI CCCGGG 1 cut(s) 75
SphI GCATGC 1 cut(s) 288
Sse9I AATT 1 cut(s) 18
SstI GAGCTC 1 cut(s) 116
StyD4I CCNGG 3 cut(s) 25, 72, 73
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 324
TaqI TCGA 1 cut(s) 116
TasI AATT 1 cut(s) 18
TatI WGTACW 1 cut(s) 197
TfiI GAWTC 1 cut(s) 182
TseFI GTSAC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 278
TspGWI ACGGA 1 cut(s) 89
TspMI CCCGGG 1 cut(s) 73
XceI RCATGY 1 cut(s) 288
XmaI CCCGGG 1 cut(s) 73
ZrmI AGTACT 1 cut(s) 199
Zsp2I ATGCAT 3 cut(s) 169, 268, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.