Rh7DG225300

SAD/SRA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0015631
Physical Location & Seq
Reverse (-)
22019882 .. 22038874
18993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG225300.1

Sequence Viewer

Length: 438 bp
ATGATAAGTTTCAGTACAGAATGGTGCTTACTATTGTCGGCTTACATCGCAGACTATGCATATTTTGGTAGGGAATCACTTGCAACCAGTGTTGTAGCATCTGGAAGATATGCTGATAATTTCCTTGATTTGAACTCCTTGACTTACACCGGTCATGGAGGAAGTGTGATGCATCATAGGCTCCCAGAAGATCAAGAGCTTAAACGTGAAAACCTTGCTATGAAGAATATGAGGGATTCAAAGAATAAAGCTGGTCAAGGAGGCCGTGGGGGTGGAAGTGGATATGGATATGGGCGAGGCCGTGGCGGTGGAGGGACCCATTATCTGCTAGGCCCTTTGAAGGGCTCAAATTTATTGATGTTGGCTGTGGAGGTGGAATTCTTTCTGAGAAACAACGATGCAGACATTCAAGATTATGACATGAAAGTCAGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
SAD_SRA

Protein Analysis

145

Amino Acids

15.96

Weight (kDa)

6.41

Isoelectric Point (pI)

31.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAD_SRA PF02182 25 - 82 6.3e-10 SAD/SRA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
AciI CCGC 1 cut(s) 306
AcsI RAATTY 2 cut(s) 349, 377
AfaI GTAC 1 cut(s) 16
AfiI CCNNNNNNNGG 2 cut(s) 340, 341
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 4 cut(s) 133, 240, 340, 410
AluBI AGCT 2 cut(s) 199, 251
AluI AGCT 2 cut(s) 199, 251
AoxI GGCC 3 cut(s) 262, 298, 331
ApoI RAATTY 2 cut(s) 349, 377
AsiGI ACCGGT 1 cut(s) 149
Asp700I GAANNNNTTC 1 cut(s) 381
AspS9I GGNCC 2 cut(s) 315, 332
AvaII GGWCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 347
BceAI ACGGC 2 cut(s) 249, 285
BfaI CTAG 1 cut(s) 329
Bme18I GGWCC 1 cut(s) 315
BmgT120I GGNCC 2 cut(s) 315, 332
BmiI GGNNCC 3 cut(s) 182, 316, 317
BmsI GCATC 4 cut(s) 107, 159, 181, 388
BsaJI CCNNGG 2 cut(s) 265, 301
BsaWI WCCGGW 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 341
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 87
BseDI CCNNGG 2 cut(s) 265, 301
BseLI CCNNNNNNNGG 2 cut(s) 340, 341
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 1 cut(s) 87
BshFI GGCC 3 cut(s) 264, 300, 333
BshTI ACCGGT 1 cut(s) 149
BsiSI CCGG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 340, 341
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 3 cut(s) 264, 300, 333
Bsp1286I GDGCHC 1 cut(s) 347
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 1 cut(s) 306
BspANI GGCC 3 cut(s) 264, 300, 333
BspCNI CTCAG 1 cut(s) 378
BspLI GGNNCC 3 cut(s) 182, 316, 317
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 1 cut(s) 87
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 2 cut(s) 265, 301
BssMI GATC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 56
BstDEI CTNAG 1 cut(s) 386
BstDSI CCRYGG 2 cut(s) 265, 301
BstKTI GATC 1 cut(s) 193
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 3 cut(s) 47, 56, 178
BsuRI GGCC 3 cut(s) 264, 300, 333
BtgI CCRYGG 2 cut(s) 265, 301
BtgZI GCGATG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 315, 332
Csp6I GTAC 1 cut(s) 15
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 2 cut(s) 155, 421
CviJI RGCY 9 cut(s) 41, 181, 199, 251, 264, 300, 333, 345, 365
CviKI_1 RGCY 9 cut(s) 41, 181, 199, 251, 264, 300, 333, 345, 365
CviQI GTAC 1 cut(s) 15
DdeI CTNAG 1 cut(s) 386
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
Eco24I GRGCYC 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 315
EcoO109I RGGNCCY 2 cut(s) 315, 332
EcoRI GAATTC 1 cut(s) 377
EcoT22I ATGCAT 2 cut(s) 61, 174
EcoT38I GRGCYC 1 cut(s) 347
FaeI CATG 2 cut(s) 158, 424
FaqI GGGAC 1 cut(s) 328
FatI CATG 2 cut(s) 154, 420
FriOI GRGCYC 1 cut(s) 347
FspBI CTAG 1 cut(s) 329
HaeIII GGCC 3 cut(s) 264, 300, 333
HapII CCGG 1 cut(s) 150
Hin1II CATG 2 cut(s) 158, 424
HinfI GANTC 2 cut(s) 74, 236
HpaII CCGG 1 cut(s) 150
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 3 cut(s) 102, 194, 410
HpyAV CCTTC 1 cut(s) 334
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 4 cut(s) 59, 83, 172, 401
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 56, 178
HpyF3I CTNAG 1 cut(s) 386
HpySE526I ACGT 1 cut(s) 205
Hsp92II CATG 2 cut(s) 158, 424
KflI GGGWCCC 1 cut(s) 315
Kzo9I GATC 1 cut(s) 190
LmnI GCTCC 1 cut(s) 186
LpnPI CCDG 5 cut(s) 87, 100, 163, 198, 237
LweI GCATC 4 cut(s) 107, 159, 181, 388
MaeI CTAG 1 cut(s) 329
MaeII ACGT 1 cut(s) 205
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MboII GAAGA 3 cut(s) 117, 200, 235
MhlI GDGCHC 1 cut(s) 347
MluCI AATT 3 cut(s) 118, 349, 377
MnlI CCTC 6 cut(s) 152, 225, 254, 290, 305, 364
Mph1103I ATGCAT 2 cut(s) 61, 174
MroXI GAANNNNTTC 1 cut(s) 381
MseI TTAA 1 cut(s) 201
MspI CCGG 1 cut(s) 150
MwoI GCNNNNNNNGC 3 cut(s) 47, 56, 178
NdeII GATC 1 cut(s) 190
NlaIII CATG 2 cut(s) 158, 424
NlaIV GGNNCC 3 cut(s) 182, 316, 317
NsiI ATGCAT 2 cut(s) 61, 174
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 2 cut(s) 74, 236
PinAI ACCGGT 1 cut(s) 149
PpuMI RGGWCCY 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 315
PspN4I GGNNCC 3 cut(s) 182, 316, 317
PspPI GGNCC 2 cut(s) 315, 332
PspPPI RGGWCCY 1 cut(s) 315
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
SaqAI TTAA 1 cut(s) 201
Sau3AI GATC 1 cut(s) 190
Sau96I GGNCC 2 cut(s) 315, 332
SduI GDGCHC 1 cut(s) 347
SetI ASST 5 cut(s) 201, 208, 216, 253, 375
SfaNI GCATC 4 cut(s) 107, 159, 181, 388
SinI GGWCC 1 cut(s) 315
Sse9I AATT 3 cut(s) 118, 349, 377
SsiI CCGC 1 cut(s) 306
SspMI CTAG 1 cut(s) 329
TaiI ACGT 1 cut(s) 208
TasI AATT 3 cut(s) 118, 349, 377
TatI WGTACW 1 cut(s) 14
TfiI GAWTC 2 cut(s) 74, 236
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 1 cut(s) 94
TspDTI ATGAA 2 cut(s) 236, 437
TspRI CASTG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 315
XapI RAATTY 2 cut(s) 349, 377
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 1 cut(s) 329
Zsp2I ATGCAT 2 cut(s) 61, 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.