Rmu_sc0005112.1_g000012

SAD/SRA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005112.1
OG0015631
Physical Location & Seq
Forward (+)
62034 .. 63855
1822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005112.1_g000012.1.cds

Sequence Viewer

Length: 690 bp
atgggtgacaaacctctattatcatcaaacattaccaagactaatgtgcaaaaaccaatagctgtgccgggagtcaagcctgcgtacgcttcatggtggaggggaaaagttgcctgcaagtctaaaccaaatggaggcatgagtccaataaaaaggcaacttgaaataaatcttcaaccagaaagatctcaaaatgactcagacaatattagaggacatactactcactttggtaatgttgttgctgctagccttcgtgatagtaatgaatctgttatccgaaagaaagtgaaagagacattggctcgattcaaagatatttgtattgagatagagagtaaaggggaatcacttgcaaccaatattgtagcatatgcaggatatgctgataatttccatgatttgaactccttgacttacaccggtcatggaggaaatcctggttttgacggcaaacgtgggcggtctttctgggcatctgtggtagtttctgtgcagggatggtgtgatccggtgactgcgaataatgggcgtctaggcgacggtgcggctagtggtggggctggttcatggcggggatcatggcatggctggttcgtggcgggactcgagggctgcgctgcacagaccactgcagcgtggtggattggtggtctgggtttgggtcaaccatgtcctgctgggccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 ko04211 map00310 map04211
Pfam Domains
Protein Families
SAD_SRA

Protein Analysis

229

Amino Acids

24.35

Weight (kDa)

9.26

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 463, 548, 574, 602
AclWI GGATC 2 cut(s) 503, 586
AcyI GRCGYC 1 cut(s) 532
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 134
AgeI ACCGGT 1 cut(s) 422
AgsI TTSAA 4 cut(s) 164, 176, 313, 406
AjnI CCWGG 1 cut(s) 439
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 1 cut(s) 62
AluI AGCT 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 290
AlwI GGATC 2 cut(s) 503, 586
Ama87I CYCGRG 1 cut(s) 608
AoxI GGCC 1 cut(s) 683
ApeKI GCWGC 4 cut(s) 245, 615, 620, 635
AsiGI ACCGGT 1 cut(s) 422
AspLEI GCGC 1 cut(s) 620
AspS9I GGNCC 1 cut(s) 683
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 2 cut(s) 17, 526
AsuNHI GCTAGC 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 608
BbvI GCAGC 4 cut(s) 232, 602, 607, 647
BccI CCATC 1 cut(s) 495
BceAI ACGGC 1 cut(s) 466
BciT130I CCWGG 1 cut(s) 441
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 290
BfaI CTAG 3 cut(s) 249, 536, 552
BfmI CTRYAG 1 cut(s) 633
BglII AGATCT 1 cut(s) 185
BisI GCNGC 5 cut(s) 246, 549, 616, 621, 636
BlsI GCNGC 5 cut(s) 247, 550, 617, 622, 637
Bme1390I CCNGG 2 cut(s) 69, 441
BmeT110I CYCGRG 1 cut(s) 608
BmgT120I GGNCC 1 cut(s) 683
BmrFI CCNGG 2 cut(s) 69, 441
BmsI GCATC 1 cut(s) 485
BmtI GCTAGC 1 cut(s) 252
BpuMI CCSGG 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 532
BsaWI WCCGGW 2 cut(s) 422, 511
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse118I RCCGGY 1 cut(s) 422
BseBI CCWGG 1 cut(s) 441
BseGI GGATG 1 cut(s) 506
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 1 cut(s) 213
BseXI GCAGC 4 cut(s) 232, 602, 607, 647
BseYI CCCAGC 1 cut(s) 680
BsgI GTGCAG 2 cut(s) 515, 606
BshFI GGCC 1 cut(s) 685
BshTI ACCGGT 1 cut(s) 422
BsiHKCI CYCGRG 1 cut(s) 608
BsiSI CCGG 3 cut(s) 68, 423, 512
BsiWI CGTACG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 618
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 290
BsmFI GGGAC 1 cut(s) 618
BsnI GGCC 1 cut(s) 685
BsoBI CYCGRG 1 cut(s) 608
Bsp143I GATC 3 cut(s) 185, 508, 578
BspACI CCGC 4 cut(s) 463, 548, 574, 602
BspANI GGCC 1 cut(s) 685
BspCNI CTCAG 1 cut(s) 212
BspMAI CTGCAG 1 cut(s) 637
BspOI GCTAGC 1 cut(s) 252
BspPI GGATC 2 cut(s) 503, 586
BsrFI RCCGGY 1 cut(s) 422
BssAI RCCGGY 1 cut(s) 422
BssMI GATC 3 cut(s) 185, 508, 578
BssNI GRCGYC 1 cut(s) 532
Bst2UI CCWGG 1 cut(s) 441
Bst4CI ACNGT 1 cut(s) 545
BstACI GRCGYC 1 cut(s) 532
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 81, 115, 250
BstDEI CTNAG 1 cut(s) 199
BstF5I GGATG 1 cut(s) 506
BstHHI GCGC 1 cut(s) 620
BstKTI GATC 3 cut(s) 188, 511, 581
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 3 cut(s) 185, 508, 578
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstNI CCWGG 1 cut(s) 441
BstSCI CCNGG 2 cut(s) 67, 439
BstSFI CTRYAG 1 cut(s) 633
BstV1I GCAGC 4 cut(s) 232, 602, 607, 647
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BsuRI GGCC 1 cut(s) 685
BtsCI GGATG 1 cut(s) 506
BtsI GCAGTG 1 cut(s) 630
BtsIMutI CAGTG 1 cut(s) 630
Cac8I GCNNGC 3 cut(s) 81, 115, 250
CfoI GCGC 1 cut(s) 620
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 683
CseI GACGC 1 cut(s) 521
Csp6I GTAC 1 cut(s) 85
CspAI ACCGGT 1 cut(s) 422
CviAII CATG 8 cut(s) 93, 139, 398, 428, 570, 582, 587, 672
CviJI RGCY 9 cut(s) 62, 79, 252, 305, 551, 563, 591, 615, 685
CviKI_1 RGCY 9 cut(s) 62, 79, 252, 305, 551, 563, 591, 615, 685
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 199
DpnI GATC 3 cut(s) 187, 510, 580
DpnII GATC 3 cut(s) 185, 508, 578
Eco88I CYCGRG 1 cut(s) 608
EcoRII CCWGG 1 cut(s) 439
FaeI CATG 8 cut(s) 96, 142, 401, 431, 573, 585, 590, 675
FaqI GGGAC 1 cut(s) 618
FatI CATG 8 cut(s) 92, 138, 397, 427, 569, 581, 586, 671
FauI CCCGC 2 cut(s) 567, 595
FauNDI CATATG 1 cut(s) 373
Fnu4HI GCNGC 5 cut(s) 246, 549, 616, 621, 636
FokI GGATG 1 cut(s) 513
Fsp4HI GCNGC 5 cut(s) 246, 549, 616, 621, 636
FspBI CTAG 3 cut(s) 249, 536, 552
GlaI GCGC 1 cut(s) 619
GluI GCNGC 5 cut(s) 246, 549, 616, 621, 636
GsaI CCCAGC 1 cut(s) 684
HaeIII GGCC 1 cut(s) 685
HapII CCGG 3 cut(s) 68, 423, 512
HgaI GACGC 1 cut(s) 521
HhaI GCGC 1 cut(s) 620
Hin1I GRCGYC 1 cut(s) 532
Hin1II CATG 8 cut(s) 96, 142, 401, 431, 573, 585, 590, 675
Hin6I GCGC 1 cut(s) 618
HinP1I GCGC 1 cut(s) 618
HincII GTYRAC 1 cut(s) 668
HindII GTYRAC 1 cut(s) 668
HinfI GANTC 7 cut(s) 72, 142, 197, 269, 309, 347, 606
HpaII CCGG 3 cut(s) 68, 423, 512
HphI GGTGA 2 cut(s) 17, 526
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 2 cut(s) 202, 281
Hpy188III TCNNGA 1 cut(s) 257
Hpy8I GTNNAC 1 cut(s) 668
Hpy99I CGWCG 1 cut(s) 545
HpyAV CCTTC 1 cut(s) 263
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4IV ACGT 1 cut(s) 457
HpyCH4V TGCA 7 cut(s) 49, 117, 356, 377, 496, 623, 635
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpyF3I CTNAG 1 cut(s) 199
HpySE526I ACGT 1 cut(s) 457
Hsp92I GRCGYC 1 cut(s) 532
Hsp92II CATG 8 cut(s) 96, 142, 401, 431, 573, 585, 590, 675
HspAI GCGC 1 cut(s) 618
Kzo9I GATC 3 cut(s) 185, 508, 578
Lsp1109I GCAGC 4 cut(s) 232, 602, 607, 647
LweI GCATC 1 cut(s) 485
MaeI CTAG 3 cut(s) 249, 536, 552
MaeII ACGT 1 cut(s) 457
MaeIII GTNAC 2 cut(s) 5, 514
MalI GATC 3 cut(s) 187, 510, 580
MboI GATC 3 cut(s) 185, 508, 578
MboII GAAGA 1 cut(s) 164
MflI RGATCY 1 cut(s) 185
MluCI AATT 1 cut(s) 391
MlyI GAGTC 4 cut(s) 81, 151, 191, 600
MnlI CCTC 6 cut(s) 24, 93, 128, 206, 425, 604
MspI CCGG 3 cut(s) 68, 423, 512
MspR9I CCNGG 2 cut(s) 69, 441
MvaI CCWGG 1 cut(s) 441
MwoI GCNNNNNNNGC 1 cut(s) 383
NciI CCSGG 1 cut(s) 69
NdeI CATATG 1 cut(s) 373
NdeII GATC 3 cut(s) 185, 508, 578
NheI GCTAGC 1 cut(s) 248
NlaIII CATG 8 cut(s) 96, 142, 401, 431, 573, 585, 590, 675
NmuCI GTSAC 2 cut(s) 5, 514
PaeR7I CTCGAG 1 cut(s) 608
PfeI GAWTC 3 cut(s) 269, 309, 347
Pfl23II CGTACG 1 cut(s) 84
PinAI ACCGGT 1 cut(s) 422
PkrI GCNGC 5 cut(s) 247, 550, 617, 622, 637
PleI GAGTC 4 cut(s) 80, 150, 191, 600
PpsI GAGTC 4 cut(s) 80, 150, 191, 600
Psp6I CCWGG 1 cut(s) 439
PspFI CCCAGC 1 cut(s) 680
PspGI CCWGG 1 cut(s) 439
PspLI CGTACG 1 cut(s) 84
PspPI GGNCC 1 cut(s) 683
PspXI VCTCGAGB 1 cut(s) 608
PstI CTGCAG 1 cut(s) 637
PsuI RGATCY 1 cut(s) 185
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SatI GCNGC 5 cut(s) 246, 549, 616, 621, 636
Sau3AI GATC 3 cut(s) 185, 508, 578
Sau96I GGNCC 1 cut(s) 683
SchI GAGTC 4 cut(s) 81, 151, 191, 600
ScrFI CCNGG 2 cut(s) 69, 441
SetI ASST 3 cut(s) 16, 64, 460
SfaNI GCATC 1 cut(s) 485
SfcI CTRYAG 1 cut(s) 633
Sfr274I CTCGAG 1 cut(s) 608
SlaI CTCGAG 1 cut(s) 608
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
Sse9I AATT 1 cut(s) 391
SsiI CCGC 4 cut(s) 463, 548, 574, 602
SspI AATATT 2 cut(s) 208, 364
SspMI CTAG 3 cut(s) 249, 536, 552
StyD4I CCNGG 2 cut(s) 67, 439
TaaI ACNGT 1 cut(s) 545
TaiI ACGT 1 cut(s) 460
TaqI TCGA 2 cut(s) 307, 609
TasI AATT 1 cut(s) 391
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 3 cut(s) 269, 309, 347
TscAI CASTG 1 cut(s) 637
TseFI GTSAC 2 cut(s) 5, 514
TseI GCWGC 4 cut(s) 245, 615, 620, 635
Tsp45I GTSAC 2 cut(s) 5, 514
TspDTI ATGAA 3 cut(s) 81, 282, 558
TspRI CASTG 1 cut(s) 637
XhoI CTCGAG 1 cut(s) 608
XspI CTAG 3 cut(s) 249, 536, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.